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Optimizing in vitro shoot propagation of Indonesian true seeds of shallot (TSS) using MS and DKW media with BAP: English
Get PDFUsing shallot botanical seeds or true seed of shallot (TSS) as planting material can produce plants free from bulb-borne disease and be more technically and economically efficient. However, its application in the field still has limitations. The objective of this study was to evaluate the in vitro propagation media containing a combination of MS and DKW media with the addition of BAP on the growth parameters and multiplication frequency as well as plantlets condition of three cultivars of TSS to initiation support the supply of quality seeds in the field. The seeds were germinated in vitro using an MS medium, and then aseptic TSS seedlings were arranged using a completely randomized design with two factors and twelve replications. The first factor was media composition. MS (Murashige and Skoog) and DKW (Driver Kuniyaki Walnut) media were used for the in vitro shoot propagation media in combination with BAP (Benzyl Amino Purine) at the level of concentrations: 0, 1, 2 and 4 mgL-1. The second factor was TSS cultivars: Bima Brebes, Trisula and Tuktuk. The result showed that Bima Brebes and Trisula cultivars significantly differed in multiplication frequency from the Tuktuk cultivar. DKW medium with 4 mgL-1 BAP produced the highest multiplication frequency but not significantly different with 4 mgL-1 BAP in MS and 2 mgL-1 BAP in DKW media. Adding 1-2 mgL-1 BAP in the DKW medium could induce new shoots on the Bima Brebes cultivar with better performance, though with lower multiplication frequency. Using shallot botanical seeds or true seed of shallot (TSS) as planting material can produce plants free from bulb-borne disease and be more technically and economically efficient. However, its application in the field still has limitations. The objective of this study was to evaluate the in vitro propagation media containing a combination of MS and DKW media with the addition of BAP on the growth parameters and multiplication frequency as well as plantlets condition of three cultivars of TSS to initiation support the supply of quality seeds in the field. The seeds were germinated in vitro using an MS medium, and then aseptic TSS seedlings were arranged using a completely randomized design with two factors and twelve replications. The first factor was media composition. MS (Murashige and Skoog) and DKW (Driver Kuniyaki Walnut) media were used for the in vitro shoot propagation media in combination with BAP (Benzyl Amino Purine) at the level of concentrations: 0, 1, 2 and 4 mgL-1. The second factor was TSS cultivars: Bima Brebes, Trisula and Tuktuk. The result showed that Bima Brebes and Trisula cultivars significantly differed in multiplication frequency from the Tuktuk cultivar. DKW medium with 4 mgL-1 BAP produced the highest multiplication frequency but not significantly different with 4 mgL-1 BAP in MS and 2 mgL-1 BAP in DKW media. Adding 1-2 mgL-1 BAP in the DKW medium could induce new shoots on the Bima Brebes cultivar with better performance, though with lower multiplication frequency.
Experimental plan for radish culture: Experimental plan
No full textOne of the main stages of agricultural testing is experimental planning. This study aimed to propose an experimental plan, with estimates of plot size, sample size and number of repetitions in the radish crop. The uniformity sowing tests were carried out in three plots of 6.5 m in length, arranged in four rows with spacing of 0.20 m between them and 0.10 m between plants, totaling 64 plants per row. The cultivars used were Redondo Vermelho Gigante and Gigante Siculo. Each plant was considered a basic experimental unit, and the number of leaves, shoot length, root circumference, plant height, root mass and shoot mass were evaluated. For each line, the plot size was estimated by the method of maximum curvature of the coefficient of variation. Sample size estimation followed Cochran\u27s methodology. To estimate the number of repetitions, the least significant difference from the Tukey test was used. It is recommended to use the largest plot size (13 plants) and sample size with twelve plants in the plot. For a minimum difference of 50% to be considered by Tukey\u27s test, between six and twelve repetitions are necessary.One of the main stages of agricultural testing is experimental planning. This study aimed to propose an experimental plan, with estimates of plot size, sample size and number of repetitions in the radish crop. The uniformity sowing tests were carried out in three plots of 6.5 m in length, arranged in four rows with spacing of 0.20 m between them and 0.10 m between plants, totaling 64 plants per row. The cultivars used were Redondo Vermelho Gigante and Gigante Siculo. Each plant was considered a basic experimental unit, and the number of leaves, shoot length, root circumference, plant height, root mass and shoot mass were evaluated. For each line, the plot size was estimated by the method of maximum curvature of the coefficient of variation. Sample size estimation followed Cochran\u27s methodology. To estimate the number of repetitions, the least significant difference from the Tukey test was used. It is recommended to use the largest plot size (13 plants) and sample size with twelve plants in the plot. For a minimum difference of 50% to be considered by Tukey\u27s test, between six and twelve repetitions are necessary
Pretreatment and drying of Peruvian Criollo mango peel and effect content of bioactive compounds and antioxidant activity
Get PDFRevaluation of Criollo mango peels for use in the food industry would generate an environmentally sustainable impact on the circular economy. This research seeks evaluation temperature (T) and time (t) of mango peel pre-cooking (PMP) and effect of drying on the concentration functional compounds (CFC). For this, a 22-type experimental design with a central point was implemented to evaluate the effects temperature (80, 89, and 98 °C) and time (5, 12.5, and 20 min) on the PMP the Criollo variety. The analyses performed included quantification total polyphenols (TP), total flavonoids (TF), antioxidant capacity (AC), and vitamin C (VC). This study, the effect conventional drying at three temperatures (45, 55, and 65 °C) was evaluated over 10 hours. The main findings study indicated that, after optimization to maximize CFC, the best values were obtained PMP of temperature and time, with 80 °C and 5 minutes, respectively. During the drying process, it was observed that, under the temperatures examined, there was no significant change in most of the samples. This study serves as a starting point for reevaluating Criollo mango skins through implementation circular economy and their application in products such as cookies made with mango peel.Revaluation of Criollo mango peels for use in the food industry would generate an environmentally sustainable impact on the circular economy. This research seeks evaluation temperature (T) and time (t) of mango peel pre-cooking (PMP) and effect of drying on the concentration functional compounds (CFC). For this, a 22-type experimental design with a central point was implemented to evaluate the effects temperature (80, 89, and 98 °C) and time (5, 12.5, and 20 min) on the PMP the Criollo variety. The analyses performed included quantification total polyphenols (TP), total flavonoids (TF), antioxidant capacity (AC), and vitamin C (VC). This study, the effect conventional drying at three temperatures (45, 55, and 65 °C) was evaluated over 10 hours. The main findings study indicated that, after optimization to maximize CFC, the best values were obtained PMP of temperature and time, with 80 °C and 5 minutes, respectively. During the drying process, it was observed that, under the temperatures examined, there was no significant change in most of the samples. This study serves as a starting point for reevaluating Criollo mango skins through implementation circular economy and their application in products such as cookies made with mango peel
Physiological performance and productivity of Tahiti lime under irrigation and rootstocks in the Amazon
Get PDFThe aim of this research was to study the physiological and productive performance of five rootstocks cultivated in the lemon ‘Tahiti CNPMF–02’ under irrigated and non-irrigated conditions in the Amazon in the sixth year of dense planting. In a completely randomized design, in a 2x5 factorial scheme (irrigation x rootstock) evaluated in the dry and rainy season, applied to the rootstocks citrumelo \u27Swingle\u27 (CTSW), lemon tree \u27Cravo Santa Cruz\u27 (LCSC), citrandarim \u27San Diego\u27(CTDI), and 2 hybrids crossing tangerine \u27Sunki Tropical\u27 and Poncirus trifoliata ‘Flying Dragon’ called TSKCxTRFD-003 (003), TSKCxTRFD–006 (006), being evaluated water relations, gas exchange, chlorophyll fluorescence, among other physiological characteristics and also the production components were. Rootstock 006 has a more efficient physiological metabolism, with and without irrigation in the face of water deficit, being more productive in the Amazon. Rootstock 003, although with good metabolic performance and adequate plant architecture, even under water deficit conditions, did not demonstrate changes in productivity. The CTDI rootstock, even when irrigated, did not demonstrate gains in productivity in the dry season, even with efficient physiological mechanisms of drought tolerance. The LCSC rootstock has less tolerance to water deficit, and even under irrigation in the dry season, it does not show improvements in its performance. However, this rootstock has high productivity in the rainy season regardless of irrigation. The CTSW rootstock must be used in restricted plantings only in management that can be irrigated, associated with annual crown adjustment pruning, avoiding densification without densification and mandatory annual crown adjustment pruning.The aim of this research was to study the physiological and productive performance of five rootstocks cultivated in the lemon ‘Tahiti CNPMF–02’ under irrigated and non-irrigated conditions in the Amazon in the sixth year of dense planting. In a completely randomized design, in a 2x5 factorial scheme (irrigation x rootstock) evaluated in the dry and rainy season, applied to the rootstocks citrumelo \u27Swingle\u27 (CTSW), lemon tree \u27Cravo Santa Cruz\u27 (LCSC), citrandarim \u27San Diego\u27(CTDI), and 2 hybrids crossing tangerine \u27Sunki Tropical\u27 and Poncirus trifoliata ‘Flying Dragon’ called TSKCxTRFD-003 (003), TSKCxTRFD–006 (006), being evaluated water relations, gas exchange, chlorophyll fluorescence, among other physiological characteristics and also the production components were. Rootstock 006 has a more efficient physiological metabolism, with and without irrigation in the face of water deficit, being more productive in the Amazon. Rootstock 003, although with good metabolic performance and adequate plant architecture, even under water deficit conditions, did not demonstrate changes in productivity. The CTDI rootstock, even when irrigated, did not demonstrate gains in productivity in the dry season, even with efficient physiological mechanisms of drought tolerance. The LCSC rootstock has less tolerance to water deficit, and even under irrigation in the dry season, it does not show improvements in its performance. However, this rootstock has high productivity in the rainy season regardless of irrigation. The CTSW rootstock must be used in restricted plantings only in management that can be irrigated, associated with annual crown adjustment pruning, avoiding densification without densification and mandatory annual crown adjustment pruning
Genetic inheritance of quantitative characters in ornamental peppers
Get PDFThis study was carried out aimed to genetic inheritance analysis of 14 characteristics in ornamental peppers. Eight ornamental Capsicum annuum lines were crossed to produce a diallel set without reciprocals. The 28 F1 hybrids and their parents were arranged in a completely randomized design, with three replicates and two plants per plot. Data were subjected to variance analysis and subsequent diallel analysis performed according to Hayman\u27s method. The t statistic was used to test the adequacy of the additive-dominance model. Canopy diameter, plant height, first bifurcation height, stem diameter, days to fruiting, leaf length, leaf width, fruit mass, fruit dry matter content, fruit diameter and yield manifested epistasis. For days to flowering and number of fruits per plant additive effects were more important, in this way selection in segregating generations will be quite efficient. These two traits exhibited partial dominance and fruit lenght exhibited overdominance it is being recommended to explore heterosis. The expected limits for the reduction of the average number of days to flowering is 89 days, for the increase in the number of fruits it is 96 fruits and for the reduction of the fruit length it is 6 milimeters. UFSJ 1 has the highest concentration of dominant genes to reduce days to flowering and fruit length averages and UFSJ 8 has the highest concentration of recessive genes to increase the number of fruit, so these parents are indicated for selection for continued breeding program.This study was carried out aimed to genetic inheritance analysis of 14 characteristics in ornamental peppers. Eight ornamental Capsicum annuum lines were crossed to produce a diallel set without reciprocals. The 28 F1 hybrids and their parents were arranged in a completely randomized design, with three replicates and two plants per plot. Data were subjected to variance analysis and subsequent diallel analysis performed according to Hayman\u27s method. The t statistic was used to test the adequacy of the additive-dominance model. Canopy diameter, plant height, first bifurcation height, stem diameter, days to fruiting, leaf length, leaf width, fruit mass, fruit dry matter content, fruit diameter and yield manifested epistasis. For days to flowering and number of fruits per plant additive effects were more important, in this way selection in segregating generations will be quite efficient. These two traits exhibited partial dominance and fruit lenght exhibited overdominance it is being recommended to explore heterosis. The expected limits for the reduction of the average number of days to flowering is 89 days, for the increase in the number of fruits it is 96 fruits and for the reduction of the fruit length it is 6 milimeters. UFSJ 1 has the highest concentration of dominant genes to reduce days to flowering and fruit length averages and UFSJ 8 has the highest concentration of recessive genes to increase the number of fruit, so these parents are indicated for selection for continued breeding program
Vegetative growth and fruit quality of \u27Tahiti\u27 acid lime grafted onto different rootstocks
Get PDFThe production of \u27Tahiti\u27 acid lime in Brazil is currently limited to the northeastern and southeastern regions and research is required to identify compatible rootstocks in order to potentiate other producing areas and to meet the needs of the consumer and industrial markets. The aim of this study was to evaluate the physical characteristics during vegetative growth and the physicochemical attributes and quality of fruit produced by \u27Tahiti CNPMF-02\u27 acid lime grafted onto the twelve rootstocks ‘Cravo LCRSC’ and ‘LCR-03’ lime, citrandarins ‘CTRI’ and ‘CTRSD’, citrumelo ‘CTSW’, ‘Pear HTR-069’ orange, ‘Volkamer LVK×LCR- 038’ lemon, and ‘Sunki TSKC×CTSW-028\u27, ‘TSKC×CTSW-041\u27, ‘TSKC×(LCR×TR)-059\u27, ‘TSKC×TRFD-003\u27 and ‘TSKT’ tangerine. The randomized experimental design included 12 treatments with four repetitions and five plants per plot. The characteristics of the grafted plants were evaluated during vegetative growth at 3.5, 4.0 and 4.5 years of age, while fruit quality was assessed at maturity. All tested rootstocks were compatible with the scion \u27Tahiti CNPMF 02\u27 acid lime cultivated under the climate and soil conditions in the north of Mato Grosso state. The rootstocks ‘LCR-003\u27, ‘CTRI’, ‘CTRSD’, ‘CTSW’ and ‘TSKC×CTSW-028\u27 induced the greatest vertical growth, trunk diameter, crown volume and vigor in the grafted plants. The physicochemical characteristics and quality of the fruit produced by grafted plants of \u27Tahiti CNPMF 02\u27 acid lime met market expectations, except for the parameter total titratable acidity. The fruits produced by \u27Tahiti CNPMF 02\u27 acid lime grafted onto ‘CTRSD’, ‘TSKC×(LCR×TR)-059’, ‘LCR-003’, ‘TSKC×CTSW-041’, ‘TSKC×TRFD-003’, ‘LVK×LCR-038’ and ‘CTRI’ were the largest in terms of length and mass.The production of \u27Tahiti\u27 acid lime in Brazil is currently limited to the northeastern and southeastern regions and research is required to identify compatible rootstocks in order to potentiate other producing areas and to meet the needs of the consumer and industrial markets. The aim of this study was to evaluate the physical characteristics during vegetative growth and the physicochemical attributes and quality of fruit produced by \u27Tahiti CNPMF-02\u27 acid lime grafted onto the twelve rootstocks ‘Cravo LCRSC’ and ‘LCR-03’ lime, citrandarins ‘CTRI’ and ‘CTRSD’, citrumelo ‘CTSW’, ‘Pear HTR-069’ orange, ‘Volkamer LVK×LCR- 038’ lemon, and ‘Sunki TSKC×CTSW-028\u27, ‘TSKC×CTSW-041\u27, ‘TSKC×(LCR×TR)-059\u27, ‘TSKC×TRFD-003\u27 and ‘TSKT’ tangerine. The randomized experimental design included 12 treatments with four repetitions and five plants per plot. The characteristics of the grafted plants were evaluated during vegetative growth at 3.5, 4.0 and 4.5 years of age, while fruit quality was assessed at maturity. All tested rootstocks were compatible with the scion \u27Tahiti CNPMF 02\u27 acid lime cultivated under the climate and soil conditions in the north of Mato Grosso state. The rootstocks ‘LCR-003\u27, ‘CTRI’, ‘CTRSD’, ‘CTSW’ and ‘TSKC×CTSW-028\u27 induced the greatest vertical growth, trunk diameter, crown volume and vigor in the grafted plants. The physicochemical characteristics and quality of the fruit produced by grafted plants of \u27Tahiti CNPMF 02\u27 acid lime met market expectations, except for the parameter total titratable acidity. The fruits produced by \u27Tahiti CNPMF 02\u27 acid lime grafted onto ‘CTRSD’, ‘TSKC×(LCR×TR)-059’, ‘LCR-003’, ‘TSKC×CTSW-041’, ‘TSKC×TRFD-003’, ‘LVK×LCR-038’ and ‘CTRI’ were the largest in terms of length and mass
Foliar application of sulfur on postharvest traits of onion hybrids
Get PDFOnion is one of the most widely consumed seasoning vegetables in the world. Beyond productivity, onion quality is of great importance, influenced by factors such as appearance, aroma, flavor, and chemical composition. This study aimed to evaluate the effect of applying elemental sulfur at different growth stages on the postharvest quality attributes of onion bulbs. Two experiments were conducted at different times using a factorial design in randomized blocks. Four hybrids were tested: Diamantina®, Optima® F1, Serena® F1, and Gamay® F1 (purple). The second factor was the timing of foliar elemental sulfur application: at the development stages of 4–6 leaves, 6–8 leaves, 8–10 leaves, and 10–12 leaves, along with a control group that received solid elemental sulfur applied to the soil. The evaluated attributes included pungency (µmol.g-1), soluble solids (°Brix), total titratable acidity (% pyruvic acid), pH, leaf sulfur content, and bulb sulfur content. Among the traits measured, the pyruvic acid content was influenced by the timing of sulfur application, with the Serena® F1 hybrid at the 10–12 leaf stage showing significant differences from the other hybrids. Regarding pungency, the Diamantina®, Serena® F1, and Gamay® F1 hybrids were classified as having medium pungency when sulfur was applied foliarly, while the Optima® F1 hybrid was classified as low/sweet pungency. Sulfur content in the bulbs and leaves of the onion cultivars was not significantly affected by foliar sulfur application.Onion is one of the most widely consumed seasoning vegetables in the world. Beyond productivity, onion quality is of great importance, influenced by factors such as appearance, aroma, flavor, and chemical composition. This study aimed to evaluate the effect of applying elemental sulfur at different growth stages on the postharvest quality attributes of onion bulbs. Two experiments were conducted at different times using a factorial design in randomized blocks. Four hybrids were tested: Diamantina®, Optima® F1, Serena® F1, and Gamay® F1 (purple). The second factor was the timing of foliar elemental sulfur application: at the development stages of 4–6 leaves, 6–8 leaves, 8–10 leaves, and 10–12 leaves, along with a control group that received solid elemental sulfur applied to the soil. The evaluated attributes included pungency (µmol.g-1), soluble solids (°Brix), total titratable acidity (% pyruvic acid), pH, leaf sulfur content, and bulb sulfur content. Among the traits measured, the pyruvic acid content was influenced by the timing of sulfur application, with the Serena® F1 hybrid at the 10–12 leaf stage showing significant differences from the other hybrids. Regarding pungency, the Diamantina®, Serena® F1, and Gamay® F1 hybrids were classified as having medium pungency when sulfur was applied foliarly, while the Optima® F1 hybrid was classified as low/sweet pungency. Sulfur content in the bulbs and leaves of the onion cultivars was not significantly affected by foliar sulfur application
Evaluation of two Bacillus sp. strains as an alternative to chemical fungicides for controlling Botrytis cinerea in roses destined for preservation
Get PDFThis study evaluated the efficacy of Bacillus subtilis and Bacillus amyloliquefaciens as biocontrol agents against Botrytis cinerea in rose crops destined for preservation in Cayambe, Ecuador. A randomized complete block design was used with nine treatments: six biological (three concentrations of each species) and three chemical treatments. The incidence and severity of the pathogen were evaluated weekly, along with economic indicators such as the cost-benefit ratio (CBR) and net benefit (NB). The biological treatment B1D3 (B. subtilis 1×10¹² CFU/ml) demonstrated the highest effectiveness, reducing incidence to 21.7% and severity to 7.61%, significantly outperforming the chemical treatments Q1, Q2, and Q3, whose values ranged between 24.2%-30.8% for incidence and 9.25%-10.80% for severity. Economically, B1D3 also stood out, achieving a CBR 10% higher and an NB approximately 40% greater than the closest chemical treatment (Q2). In contrast, lower-concentration biological treatments, such as B2D1 (B. amyloliquefaciens 1×10⁶ CFU/ml), were less effective, with incidences of 40.4% and severities of 21.6%. These results position B1D3 as a sustainable and economically viable alternative to traditional chemical fungicides, reinforcing its utility for integrated management of B. cinerea. This study supports the use of Bacillus as an effective and cost-efficient strategy that enhances the sustainability of floriculture while reducing the environmental impact associated with synthetic chemical use.This study evaluated the efficacy of Bacillus subtilis and Bacillus amyloliquefaciens as biocontrol agents against Botrytis cinerea in rose crops destined for preservation in Cayambe, Ecuador. A randomized complete block design was used with nine treatments: six biological (three concentrations of each species) and three chemical treatments. The incidence and severity of the pathogen were evaluated weekly, along with economic indicators such as the cost-benefit ratio (CBR) and net benefit (NB). The biological treatment B1D3 (B. subtilis 1×10¹² CFU/ml) demonstrated the highest effectiveness, reducing incidence to 21.7% and severity to 7.61%, significantly outperforming the chemical treatments Q1, Q2, and Q3, whose values ranged between 24.2%-30.8% for incidence and 9.25%-10.80% for severity. Economically, B1D3 also stood out, achieving a CBR 10% higher and an NB approximately 40% greater than the closest chemical treatment (Q2). In contrast, lower-concentration biological treatments, such as B2D1 (B. amyloliquefaciens 1×10⁶ CFU/ml), were less effective, with incidences of 40.4% and severities of 21.6%. These results position B1D3 as a sustainable and economically viable alternative to traditional chemical fungicides, reinforcing its utility for integrated management of B. cinerea. This study supports the use of Bacillus as an effective and cost-efficient strategy that enhances the sustainability of floriculture while reducing the environmental impact associated with synthetic chemical use
Seedling performance-based methodologies for assessing melon seed physiological quality
Get PDFWhile numerous methods exist for assessing seed physiological quality, seedling performance-based approaches often lack species-specific adaptations. This study evaluated the efficacy of various seedling performance methodologies for assessing physiological quality in yellow Valenciano-type melon (Cucumis melo L.) seed lots. Four seed lots underwent comprehensive testing, including: water content, germination, first count, electrical conductivity, root dry mass, hypocotyl/primary root length, greenhouse emergence, and multiple germination/emergence velocity indices (original and corrected forms). Strong correlations emerged between velocity indices and laboratory tests, though these relationships disappeared under field conditions. Our findings demonstrate that seedling-based assessments effectively distinguish melon seed vigor in controlled laboratory environments, but lose predictive validity in field applications due to environmental interactions. These results highlight the need for species-specific vigor protocols and caution against extrapolating laboratory results to field performance predictions.While numerous methods exist for assessing seed physiological quality, seedling performance-based approaches often lack species-specific adaptations. This study evaluated the efficacy of various seedling performance methodologies for assessing physiological quality in yellow Valenciano-type melon (Cucumis melo L.) seed lots. Four seed lots underwent comprehensive testing, including: water content, germination, first count, electrical conductivity, root dry mass, hypocotyl/primary root length, greenhouse emergence, and multiple germination/emergence velocity indices (original and corrected forms). Strong correlations emerged between velocity indices and laboratory tests, though these relationships disappeared under field conditions. Our findings demonstrate that seedling-based assessments effectively distinguish melon seed vigor in controlled laboratory environments, but lose predictive validity in field applications due to environmental interactions. These results highlight the need for species-specific vigor protocols and caution against extrapolating laboratory results to field performance predictions
Weed interference on organic carrot yield under different sowing methods
Get PDFWeed cause serious problems to vegetable cultivation, justifying the importance of studies on the interference of these plants in agricultural crops and management techniques in the context of organic production. From this perspective, this study aimed to evaluate the interference of weed on the yield of carrot grown under different sowing methods. The study was conducted at the Seridó Ecological Station in Rio Branco, Acre, Brazil. Control and coexistence experiments with weeds were set up in a split-plot design (5 x 2), with the plot corresponding to the coexistence or control periods: 15, 20, 25, 30, and 35 days after sowing, and the subplots corresponding to the sowing methods: direct seeding and water conditioning. Cultivation followed the recommendations for carrot and for the organic system. The harvest and evaluations were performed after 80 days by evaluating the biometric variables, the carrot yield, and the weed mass. The statistical analysis consisted of the test of assumptions followed by analysis of variance. Non-linear regression was used for the yield parameters by determining the interference periods. In contrast, linear regression was used for the other variables. Carrot coexistence with weeds reduced the conventional commercial yield by 75.73% and the organic commercial yield by 57.07%. Organic carrot cultivation should occur free of weed from 21 to 28 days. Water conditioning increased the organic carrot yield.Weed cause serious problems to vegetable cultivation, justifying the importance of studies on the interference of these plants in agricultural crops and management techniques in the context of organic production. From this perspective, this study aimed to evaluate the interference of weed on the yield of carrot grown under different sowing methods. The study was conducted at the Seridó Ecological Station in Rio Branco, Acre, Brazil. Control and coexistence experiments with weeds were set up in a split-plot design (5 x 2), with the plot corresponding to the coexistence or control periods: 15, 20, 25, 30, and 35 days after sowing, and the subplots corresponding to the sowing methods: direct seeding and water conditioning. Cultivation followed the recommendations for carrot and for the organic system. The harvest and evaluations were performed after 80 days by evaluating the biometric variables, the carrot yield, and the weed mass. The statistical analysis consisted of the test of assumptions followed by analysis of variance. Non-linear regression was used for the yield parameters by determining the interference periods. In contrast, linear regression was used for the other variables. Carrot coexistence with weeds reduced the conventional commercial yield by 75.73% and the organic commercial yield by 57.07%. Organic carrot cultivation should occur free of weed from 21 to 28 days. Water conditioning increased the organic carrot yield