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Monitoring the Seasonal Presence of the Blue Shiner, Cyprinella caerulea, in Little River Canyon National Preserve using Environmental DNA
The study of environmental DNA (eDNA) has provided researchers with a more accessible and sensitive way to identify the presence of specific species compared to traditional monitoring methods. eDNA enables species detection by analyzing environmental samples such as water or soil, which contain genetic material shed by organisms in a given area. Research indicates that eDNA techniques have become a valuable method for monitoring threatened and invasive species, proving particularly reliable for detecting aquatic species compared to traditional techniques. Quantitative PCR (qPCR) has been used in numerous eDNA studies, as it provides for greater accuracy than conventional PCR. Cyprinella caerulea, commonly known as the blue shiner, is a federally-listed (threatened) fish species that was once abundant in many watersheds across the southern United States. In this study, we surveyed six sites in Little River, specifically within Little River Canyon National Preserve in Alabama. Previous studies on C. caerulea indicated their presence within this watershed. Any amplification of C. caerulea DNA was analyzed using computer software and displayed on real-time graphs, with results compared based on the corresponding season of collection and any variation at sites. Our results illustrate the effectiveness of eDNA detection for monitoring threatened species, suggesting that all future studies of C. caerulea and other endangered species could benefit from this same process. Although this study shows that history can serve as a starting point for developing monitoring surveys over time, future studies should evaluate detection probability across various interactions, focusing on seasonality and organismal behavior over extended periods of collection times
Quoted Message Poster 6
Eight of these posters (dimensions 24x35 inches) feature quotes from renowned designers that support the research and objectives of the proposed methodology.https://digitalcommons.jsu.edu/etds_mfa_images/1116/thumbnail.jp
Disclaimer
On the right side of the gallery, a poster (dimensions 24x35 inches) with a disclaimer explains the reasoning and potential applications of the methodology.https://digitalcommons.jsu.edu/etds_mfa_images/1119/thumbnail.jp
JSU ROTC, 2024 Alumni Banquet 23
The 29th annual ROTC Homecoming Banquet at Jacksonville State University was held September 20, 2024 in the Recreation and Fitness Center. Shown is speaker Col. (Ret) Jason Benefield.https://digitalcommons.jsu.edu/rotc_photos/11082/thumbnail.jp
JSU ROTC, 2024 Alumni Banquet 29
The 29th annual ROTC Homecoming Banquet at Jacksonville State University was held September 20, 2024 in the Recreation and Fitness Center. Shown is a table of door prizes.https://digitalcommons.jsu.edu/rotc_photos/11088/thumbnail.jp
JSU ROTC, 2024 Alumni Banquet 32
The 29th annual ROTC Homecoming Banquet at Jacksonville State University was held September 20, 2024 in the Recreation and Fitness Center. Shown are guests.https://digitalcommons.jsu.edu/rotc_photos/11091/thumbnail.jp
JSU ROTC, 2024 Alumni Banquet 39
The 29th annual ROTC Homecoming Banquet at Jacksonville State University was held September 20, 2024 in the Recreation and Fitness Center. Shown guests fill plates with food in the buffet line. Identified are Col. Manual F. Ramirez and President and Mrs. Don Killingsworth.https://digitalcommons.jsu.edu/rotc_photos/11098/thumbnail.jp
JSU ROTC, 2024 Alumni Banquet 41
The 29th annual ROTC Homecoming Banquet at Jacksonville State University was held September 20, 2024 in the Recreation and Fitness Center. Collin Benoit received the Elite Lettermen\u27s Club ROTC leadership scholarship in memory of Bill Stone. LTC William F. Huggins stands with him.https://digitalcommons.jsu.edu/rotc_photos/11100/thumbnail.jp
JSU ROTC, 2024 Alumni Banquet 50
The 29th annual ROTC Homecoming Banquet at Jacksonville State University was held September 20, 2024 in the Recreation and Fitness Center. Shown Col. Michael Dalesandro makes a presentation to Matthew Henson.https://digitalcommons.jsu.edu/rotc_photos/11109/thumbnail.jp