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Chondrostoma smyrnae, a new nase from the Tahtali reservoir drainage in the Aegean Sea basin (Teleostei, Leuciscidae)
Full text linkChondrostoma smyrnae, a new species, from the Tahtali reservoir drainage is distinguished by having a slightly arched lower jaw with a well-developed keratinised edge, a deep and cylindric body, a complete lateral line with 47-52+1 total scales, 8-9 scale rows between the lateral line and the dorsal-fin origin, 4 scale rows between the lateral line and the pelvic fin-origin, and 19-23 gill rakers on the first gill arch. Moreover, molecular analyses using full cyt b (1141 bp) and partial coI (652 bp) sequences of the mitochondrial genome from specimens of the new species, C. smyrnae and specimens belonging to other Chondrostoma species from central and western Anatolia demonstrated that the C. smyrnae is easily differentiated by their high pairwise genetic distances of cyt b and coI data set (>2.20 and 1.03%, respectively) and by their position in the phylogenetic trees obtained through Maximum Likelihood (ML) methodology
Decoding 17-beta-hydroxysteroid dehydrogenase 13: a multifaceted perspective on its role in hepatic steatosis and associated disorders
Full text linkChronic liver disease (CLD) represents a significant global health burden, with hepatic steatosis-associated disorders-such as metabolic dysfunction-associated steatohepatitis (MASH), alcoholic liver disease, and hepatitis C virus infection-being major contributors. Recent genome-wide association studies have identified the rs72613567:TA variant in the 17-beta-hydroxysteroid dehydrogenase 13 ( HSD17813 ) gene as a protective factor against the development and progression of these conditions. In this review, we summarized the current evidence surrounding the HSD17813 rs72613567 variant, aiming to elucidate its impact on CLD risk and outcomes, and to explore the potential mechanisms behind its hepatoprotective effects. The rs72613567:TA variant induces a splice donor site mutation, resulting in a truncated, nonfunctional HSD17B13 protein. Numerous studies have demonstrated that this loss-of-function mutation confers protection against the development of cirrhosis and hepatocellular carcinoma (HCC) in patients with MASH, alcoholic liver disease, and hepatitis C virus infection. Moreover, the rs72613567:TA variant has been associated with reduced liver enzyme levels and improved survival in HCC patients. Integrating this variant into genetic risk scores has shown promise in predicting the progression of fatty liver disease to cirrhosis and HCC. Furthermore, inhibiting HSD17813 expression through RNA interference and small molecule inhibitors has emerged as a potential therapeutic strategy for MASH. However, the precise molecular mechanisms underlying the hepatoprotective effects of the HSD17813 rs72613567 variant remain to be fully elucidated. Future research should focus on clarifying the structure-function relationship of HSD17813 and its role in liver pathophysiology to facilitate the development of targeted therapies for CLD associated with hepatic steatosis
Codon usage bias of the polyphenol oxidase genes in camellia sinensis: a comprehensive analysis
No full textTea, a widely consumed beverage globally, is a vital agricultural product for many countries. Polyphenol oxidases (PPOs), copper-containing enzymes found in plants, fungi, and animals, are essential for physiological metabolism and enzymatic browning in tea plants (Camellia sinensis). Codon usage bias (CUB), a key evolutionary characteristic, offers valuable insights into species evolution and gene function. However, the codon usage patterns of Camellia sinensis polyphenol oxidase (CsPPO) genes remain undocumented. In this study, we conducted, for the first time, a comprehensive analysis of CUB in 24 CsPPO genes, comparing their CUB profiles with those of other Camellia species (Camellia lanceoleosa, Camellia nitidissima, Camellia ptilophylla) and non-Camellia species (Actinidia chinensis, Cornus florida, Rhododendron vialii) to elucidate potential evolutionary relationships and functional constraints influencing CUB. Nucleotide composition analysis revealed an AT-rich bias, with a preference for G/C-ending codons at the third position. Codon usage indices indicated low expression levels and weak CUB. RSCU and RFSC analyses revealed that the preferred and high-frequency codons were mostly G/C-ending. Codon usage frequency analysis suggested Zea mays as a suitable host for CsPPO gene expression. ENC-GC3s, PR2, and neutrality plots showed natural selection had a stronger impact than mutation on CUB. Additionally, measure independent of length and composition (MILC) values confirmed low PPO gene expression levels, and correlation analyses demonstrated that both nucleotide composition and gene expression affect CUB. Overall, codon usage in CsPPO genes is mainly shaped by natural selection, with weak bias and low expression potential, providing useful insights for future genetic engineering and heterologous expression