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Dittrichia graveolens (Asteraceae), rapidly spreading along European highways, recorded in Lithuania /
No full textFor a long time, railways were the main pathway for the accidental introduction of alien plant species, but now highways and other roads are becoming increasingly important in this process. Considering that Dittrichia graveolens is spreading rapidly along highways in Central Europe and neighbouring Poland, a targeted search was conducted in Lithuania in 2023. Dittrichia graveolens was recorded at two sites along the Kalvarija–Kaunas highway (Kazlų Rūda district, in the environs of Pentupiai, and Kaunas city, in the environs of Jonučiai). At both sites, the species occupied relatively compact stands consisting of several hundred individuals, suggesting that the stands were formed from locally matured seeds. The mean number of flowers per capitulum in Lithuania in 2023 was 20.5 ± 4.9, and the realised fecundity was 89.7% of the potential fecundity. It is presumed that the seeds of Dittrichia graveolens were accidentally introduced into Lithuania by trucks, probably attached to tyres. Currently, this species is classified as a casual alien, but its naturalisation is expected. The study revealed that the first record of Dittrichia graveolens in Poland was not in 2013, as previously reported, but more than 20 years earlier, in 1990, near Kraków, at the slag deposit site of the Nowa Huta Steelworks
Assessment of heavy metal contamination in dust in Vilnius schools: source identification, pollution levels, and potential health risks for children /
No full textThe main objective of this study is to thoroughly evaluate the diversity and sources of heavy metals in the school environment. Specifically, this study examines the presence of heavy metals in the dust found and collected from 24 schools in Vilnius. Employing hierarchical cluster analysis, principal component analysis, and positive matrix factorization, we identified combustion-related activities as primary contributors to elevated metal concentrations, notably zinc, scandium, and copper, with PM2.5/PM10 ratios indicating a combustion source. They reveal significant differences in the levels of elements such as arsenic (4.55–69.96 mg/kg), copper (51.28–395.37 mg/kg), zinc, and lead, which are affected by both local environmental factors and human activities. Elevated pollution levels were found in certain school environments, indicating environmental degradation. Pollution assessment and specific element pairings’ strong positive correlations suggested shared origins or deposition processes. While this study primarily assesses non-carcinogenic risks to children based on a health risk assessment model, it acknowledges the well-documented carcinogenic potential of substances such as lead and arsenic. The research emphasizes the immediate necessity for efficient pollution management in educational environments, as indicated by the elevated hazard index for substances such as lead and arsenic, which present non-carcinogenic risks to children. This research offers important insights into the composition and origins of dust pollution in schools. It also promotes the need for broader geographic sampling and prolonged data collection to improve our understanding of pollution sources, alongside advocating for actionable strategies such as environmental management and policy reforms to effectively reduce exposure risks in educational settings. Furthermore, it aims to develop specific strategies to safeguard the health of students in Vilnius and similar urban areas
The genus Branchiobdella Odier, 1823 (Annelida: Branchiobdellida) in Lithuania, with an overview and an identification key to the species in the Baltic countries /
No full textBranchiobdellidans dwelling on Astacus astacus (Linnaeus, 1758) from 13 localities in Lithuania were collected in 2018 and 2020. Four Branchiobdella species were identified: B. astaci Odier, 1823, B. pentadonta Whitman, 1882, B. kozarovi Sucbhev, 1978 and B. bulgariensis Subchev & Rimcheska, 2021. Specimens of B. astaci were found only in two localities, lakes Ramis and Krakavas. The most widely distributed species is B. pentadonta being found on crayfish at all localities investigated, with the exception of Lake Kaukinė. The latter locality is the only one where crayfish were infested by B. kozarovi. B. bulgariensis was only found at one locality, and possibly at a second, and A. astacus is a new host for B. bulgariensis. B. kozarovi and B. bulgariensis are new to the Lithuanian fauna list. An overview of the genus Branchiobdella in the Baltic countries and a key to its species are also presented
Exploring the effects of graphene-based nanoparticles on early salmonids cardiorespiratory responses, swimming and nesting behavior /
No full textGraphene-based nanomaterials are exceptionally attractive for a wide range of applications, raising the likelihood of the release of graphene-containing nanoparticles into aquatic environments. The growing use of these carbon nanomaterials in different industries highlights the crucial need to investigate their environmental impact and evaluate potential risks to living organisms. The current investigation evaluated the nanotoxicity of graphene (nanoflakes) and graphene oxide (GO) nanoparticles on the cardiorespiratory responses (heart rate, gill ventilation frequency), as well as the swimming and nesting behavioral parameters of early stage larvae and juvenile salmonids. Both short-term (96 h) and long-term (23 days) exposure experiments were conducted using two common species: brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss). The findings demonstrated notable alterations in fish nesting behavior, swimming performance, and cardiorespiratory functions, indicating the potential toxicity of nanoparticles. This impact was observed at both physiological and whole-organismal levels in salmonids at early stages. Future investigations should explore different types of nanocarbons and their potential enduring effects on fish population structure, considering not only individual survival but also broader aspects of development, including feeding, reproductive, and other social dynamics
Comparative phytoprofiling of Achillea millefolium morphotypes: Assessing antioxidant activity, phenolic and triterpenic compounds variation across different plant parts /
No full textAchillea millefolium L., commonly known as yarrow, is a versatile and widely distributed plant species with a rich history of ethnopharmacological significance. This study aimed to evaluate the comparative differences of A. millefolium inflorescence morphotypes. The phytochemical profile of white and pink inflorescence morphotypes was characterised by a complex of thirty-four phenolic and triterpene compounds. The species has distinct morphotypes of white and pink inflorescence. Phenolic and triterpenic profiles were determined, and individual compounds were quantified in inflorescence, leaf, and stem samples of two morphotypes tested. The antioxidant activity of plant extracts was evaluated by free radical scavenging (ABTS) and ferric-reducing antioxidant power (FRAP) assays. Caffeoylquinic acids predominated in all parts of the plant tested. Chlorogenic acid and 3,5-dicaffeoylquinic acid were the principal compounds in the phenolic profile. Betulin, betulinic acid, and α-amyrin were the prevailing triterpenic components in the triterpenic profiles of Achillea millefolium morphotypes. The predominant flavonoids in inflorescences were flavones, while in leaves, flavonols were the organ-specific compounds. The quantitative differences were observed between plant parts of morphotypes. Leaves consistently displayed the highest amounts of identified compounds and have been testified as the main source of antioxidant activity. Overall, white inflorescences accumulated a higher total amount of compounds compared to pink ones. The observed differences between morphotypes derived from the same population reflect the differences in specialised metabolites and their chemotypes. This study addresses gaps in knowledge, particularly in phenolic and triterpenic profiling of coloured inflorescence morphotypes, enhancing our understanding of chemotypes and morphotypes within the species
Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites /
No full textAvian haemosporidian parasites are useful model organisms to study the ecology and evolution of parasite-host interactions due to their global distribution and extensive biodiversity. Detection of these parasites has evolved from microscopic examination to PCR-based methods, with the mitochondrial cytochrome b gene serving as barcoding region. However, standard PCR protocols used for screening and identification purposes have limitations in detecting mixed infections and generating phylogenetically informative data due to short amplicon lengths. To address these issues, we developed a novel genus-specific nested PCR protocol targeting avian haemosporidian parasites. The protocol underwent rigorous testing utilizing a large dataset comprising blood samples from Malagasy birds of three distinct Passeriformes families. Furthermore, validation was done by examining smaller datasets in two other laboratories employing divergent master mixes and different bird species. Comparative analyses were conducted between the outcomes of the novel PCR protocol and those obtained through the widely used standard nested PCR method. The novel protocol enables specific identification of Plasmodium, Haemoproteus (Parahaemoproteus), and Leucocytozoon parasites. The analyses demonstrated comparable sensitivity to the standard nested PCR with notable improvements in detecting mixed infections. In addition, phylogenetic resolution is improved by amplification of longer fragments, leading to a better understanding of the haemosporidian biodiversity and evolution. Overall, the novel protocol represents a valuable addition to avian haemosporidian detection methodologies, facilitating comprehensive studies on parasite ecology, epidemiology, and evolution
New Afrotropical Opisthoheza siniaevi and Sonithometa maurice (Lepidoptera, Lasiocampidae, Lasiocampinae, Gastropachini) /
No full textOne new species of the genus Opisthoheza Zolotuhin & Prozorov, 2010, Opisthoheza siniaevi sp. n. from the Republic of Congo, is described together with closely related Sonithometa maurice gen & sp. n. from Guinea. The new species are compared with Opisthoheza heza Zolotuhin & Prozorov, 2010 and Nirbiana obscura (Hering, 1941). Male adults and genitalia are illustrated
Cryptic species complex shows population-dependent, rather than lineage-dependent tolerance to a neonicotinoid /
No full textCryptic species are rarely considered in ecotoxicology, resulting in misleading outcomes when using a single morphospecies that encompasses multiple cryptic species. This oversight contributes to the lack of reproducibility in ecotoxicological experiments and promotes unreliable extrapolations. The important question of ecological differentiation and the sensitivity of cryptic species is rarely tackled, leaving a substantial knowledge gap regarding the vulnerability of individual cryptic species within species complexes. In times of agricultural intensification and the frequent use of pesticides, there is an urgent need for a better understanding of the vulnerability of species complexes and possible differences in adaptive processes. We used the cryptic species complex of the aquatic amphipod Gammarus roeselii, which comprises at least 13 genetic mtDNA lineages and spans from small-scale endemic lineages in Greece to a large-scale widely distributed lineage in central Europe. We exposed eleven populations belonging to four lineages to the neonicotinoid thiacloprid in an acute toxicity assay. We recorded various environmental variables in each habitat to assess the potential pre-exposure of the populations to contaminants. Our results showed that the populations differed up to 4-fold in their tolerances. The lineage identity had a rather minor influence, suggesting that the cryptic species complex G. roeselii does not differ significantly in tolerance to the neonicotinoid thiacloprid. However, the observed population differentiation implies that recent pre-exposure to thiacloprid (or similar substances) or general habitat contamination has triggered adaptive processes. Though, the extent to which these mechanisms are equally triggered in all lineages needs to be addressed in the future. Our study provides two key findings: Firstly, it shows that observed phylogenetic differences within the G. roeselii species complex did not reveal differences in thiacloprid tolerance. Second, it confirms that differentiation occurs at the population level, highlighting that susceptibility to toxicants is population-dependent. The population-specific differences were within the range of accepted intraspecific variability from a regulatory standpoint. From an evolutionary-ecological perspective, it remains intriguing to observe how persistent stresses will continue to influence tolerance and whether different populations are on distinct pathways of adaptation. Given that the potential selection process has only lasted a relatively short number of generations, it is crucial to monitor these populations in the future, as even brief exposure periods significantly impact evolutionary responses
Inter- and intra-annual carbon isotope fluctuations in Pinus sylvestris L. tree rings whole wood and cellulose in north-eastern Lithuania /
No full textIn temperate regions trees typically exhibit growth sensitivity to climatic conditions during the growth season. Annual tree ring growth increments correlate with a variety of environmental factors. As an index of water use efficiency, δ13C is a preferred proxy to allow accurate interpretation of environmental factors critical for the tree growth, including changes in climate patterns, variation in the ambient temperature and precipitation. We assumed that isotopic differences within individual tree rings are likely to produce seasonal isotope fluctuations in the chronology that might be interpreted as response to environmental impacts. To verify the assumption, we measured δ13C in annual tree rings of Pinus Sylvestris L. split into 13 intra-annual segments each and checked δ13C correlations with temperature, precipitation, the number of sunshine hours and relative air humidity. For the investigation, we chose a site in north-eastern Lithuania, Zarasai, located in boreal latitude and remote from industrial pollution sources. The methodology of the research was based on high coherence of δ13C chronologies measured in the whole wood and α-cellulose extracted by means of two different methods. The experiment produced strong δ13C correlations with hydrometeorological parameters, especially in the earlywood formed in June