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Prevalence, morphological and molecular characterization of Leucocytozoon macleani (Apicomplexa: Haemosporida) from chickens in Thailand /
No full textLeucocytozoon species are common in countries with warm climates but are an often neglected blood parasite in poultry. Although Leucocytozoon macleani is less virulent than Leucocytozoon caulleryi, it can still negatively impact production performance. In Thailand, the available reports indicate a high prevalence of Leucocytozoon spp., but detailed morphological characteristics of the parasites remain insufficiently known. In this study, Giemsa-stained blood smears and extracted genomic (g) DNA were obtained from 60 domestic chickens (Gallus gallus domesticus). Blood smears were examined for the presence of Leucocytozoon species and their morphological characteristics were examined. A total of 60 gDNA samples were used for nested-PCR amplification of the cytochrome b gene of Leucocytozoon species, followed by sequencing and phylogenetic analysis. The microscopic and molecular examinations revealed prevalence of leucocytozoonosis in chickens of 85% and 90%, respectively. Sequence analysis indicated that several infected chickens harboured multiple Leucocytozoon lineages. Leucocytozoon macleani was morphologically identified in nine samples and could be linked to the lineages GALLUS17, GALLUS34, and the new lineages GALLUS63. The found gametocytes of L. macleani morphologically resembled those reported previously, but exhibited some distinct characteristics. Phylogenetically, the lineages of L. macleani isolated in this study grouped separately from some other L. macleani lineages deposited in GenBank. In conclusion, the prevalence of Leucocytozoon infection in chickens from Northeastern Thailand was high, with frequent co-infections by multiple lineages. Leucocytozoon macleani may exhibit cryptic specification. This study is the first report of L. macleani lineages described using MalAvi database nomenclature, alongside their morphological characteristics
Impact of phytoplankton biomass on the growth and development of agricultural plants /
No full textThe agricultural sector plays one of the pivotal roles in fulfilling the objectives set forth by the EU Green Deal. However, the extensive use of synthetic fertilizers has contributed to nutrient over-enrichment in aquatic ecosystems, promoting eutrophication due to excess nitrogen inputs from fertilizers. This phenomenon is a key driver of rapid and excessive algal blooms in rivers, lakes, and seas. In this study, three globally cultivated crop species—oilseed rape (Brassica napus L.), common wheat (Triticum aestivum L.), and pea (Pisum sativum L.)—were selected for experimental analysis, including the assessment of biochemical parameters such as proline content, lipid peroxidation levels, hydrogen peroxide production, total phenol content, and antioxidant activity, which were evaluated to determine the potential of phytoplankton biomass as a substitute for synthetic fertilizers. Various quantities of lyophilized phytoplankton biomass, collected from the Curonian Lagoon, a biologically productive and ecologically sensitive brackish water body in the southeastern Baltic region, were incorporated into the growth substrates of the studied plants. The findings indicate that utilizing excess phytoplankton biomass can serve not only as a plant growth biostimulant but also as a sustainable alternative to synthetic fertilizers, thereby contributing to improved water quality and more environmentally responsible agricultural practices
First investigation of Haemosporidian species and record of novel genetic lineages in Eurasian griffon vultures (Gyps fulvus) in Greece /
No full textEurasian griffon vultures (Gyps fulvus) are endangered scavenger birds. Haemosporidian parasites infect the blood cells and organs of many avian species globally, using blood-sucking insect vectors, and they negatively affect health of birds and subsequently wildlife biodiversity. Fifty-nine vultures were admitted to the Greek wildlife rehabilitation center “ANIMA” and included in this study. Blood samples were collected, and the microscopy of stained blood smears was performed. Moreover, DNA was extracted, samples were screened for Leucocytozoon, Haemoproteus, and Plasmodium spp. following nested PCR protocols, and positive samples were sequenced. The detected haemosporidians are reported for the first time in Eurasian griffon vultures globally. The overall prevalence was 11.9% (Leucocytozoon spp. 5.1%, Haemoproteus spp. 5.1%, Plasmodium spp. 3.4%); this further corroborates the statement that the vultures’ immunity protects them from blood parasites. Notably, new genetic lineages of Leucocytozoon (GYPFUL02), Haemoproteus (GYPFUL01), and Plasmodium (GYPFUL03) species were recorded for the first time. Furthermore, it was the first molecular isolation of Haemoproteus in Old World vultures and Haemoproteus brachiatus was isolated for the first time from a vulture species. Moreover, this demonstrates the first record of Parahaemoproteus genetic lineages in vultures. Results highlight the genetic diversity of haemosporidians in griffon vultures and the need for additional studies
Divergent trends in insect disturbance across Europe's temperate and boreal forests /
No full textOngoing shifts in climate and land use have altered interactions between trees and insect herbivores, changing biotic disturbance regimes. However, as these changes are complex and vary across host species, insect taxa, and feeding guilds, they remain poorly understood. We compiled annual records of forest insect disturbance from 15 countries in temperate and boreal Europe, spanning the period from 2000 to 2022. The dataset comprises 1361 time series characterizing the dynamics of 50 herbivorous insects. We used this dataset to test whether insect disturbance has systematically changed during the 23-year period across host trees and feeding guilds, whether it varies along latitudinal and climatic gradients, and whether synchrony exists among species in the same guild or among species sharing the same host. Since 2000, borer disturbance was predominantly concentrated on gymnosperms, while defoliators impacted gymnosperms and angiosperms more evenly. While 85.8% of gymnosperm disturbance was inflicted by a single species, Ips typographus, the majority of disturbances to angiosperms were caused by six different species. Borer impact on gymnosperms has increased in the 21st century, while defoliator impact has decreased across both clades. In contrast to diverging temporal trends, disturbance was consistently greater in warmer and drier conditions across feeding guilds and host types. We identified significant synchrony in insect disturbance within host types and feeding guilds but not between these groups, suggesting shared drivers within guilds and host types. Increasing insect disturbance to gymnosperms may catalyze adaptive transformations in Europe's forests, promoting a shift from historical conifer-dominated management to broadleaved trees, which are less affected by insect herbivores. Our findings reveal a diversity of trends in insect herbivory, underscoring the need to strengthen monitoring and research in order to better understand underlying mechanisms and identify emerging threats that may not be apparent in currently available data
Fluid-mediated alteration of titanite in the Kabeliai granites, SE Lithuania and comparison with Bulgarian granitoid systems /
No full textKnowledge and use of bee products in Lithuania /
No full textBackground/Objective: In recent years, there has been a growing public interest in natural products, including those derived from bees. While most scientific research on bee products has focused on their pharmacological properties, insufficient attention has been given to consumer knowledge, consumption habits and attitudes. The aim of this study was to estimate the popularity of use of different bee products and assess consumer knowledge about them in Lithuania. Methods: An online survey was carried out of the general adult population of Lithuania with 421 respondents included. Results: The study revealed that honey, beeswax and royal jelly were the best-known bee products, while bee venom was the least known one. Knowledge levels varied by age and occupation of respondents—older people and those working in pharmacy, healthcare, cosmetology, agriculture, beekeeping and food production showed better perception. Honey was most often used for treating colds (78.9%), prevention (78.1%) and reducing fever (65.3%). Dietary use of honey depended on demographic factors and was generally low—28.3% consumed it only a few times per year or less. For cosmetics, propolis was the most used product (34.2%). Conclusions: Older individuals and professionals in pharmacy, healthcare, cosmetology, agriculture, beekeeping and food production, demonstrated better knowledge of bee products. Although honey was rarely consumed as part of the diet, older people tended to use it more often than younger individuals. Men were more likely to use honey for treating digestive and circulatory issues and as an ingredient in food and beverages, whereas women were more inclined to use it for respiratory illnesses and cosmetic treatments
The ecological drivers of the mosaic structure of bryophyte and vascular plant cover in the rich fens of Lithuania /
No full textThe composition and structure of vegetation have been recognised as the main determinants of habitat quality, which influences biodiversity. The presented research focuses on the mosaic structure of Lithuanian rich fens and their relationship to ecological conditions. This study was conducted across 98 study plots amongst 15 fens distributed throughout Lithuania. This research included the cover and abundance of vascular plants and bryophytes, water parameters (conductivity, pH, and concentrations of Ca2+, Fe3+, K+, Mg2+, NH4+, NO3−, and PO43−), topography type, and the cover of hummocks. Vegetation studies resulted in the distinction of two clusters containing ten bryophyte groups and two clusters containing eleven vascular plants groups. The main diagnostic species for bryophyte clusters were Scorpidium cossonii and Calliergonella cuspidata, and those for the vascular plant clusters were Carex lepidocarpa and Carex rostrata. The mosaic distribution of vegetation observed in both the bryophyte and vascular plant layers is primarily shaped by local hydrological regimes, microtopographical variation, and the amount of iron present. The habitats of bryophyte groups, as compared to those of vascular plants, were determined by narrower ecological conditions. This study emphasised the specificity of Lithuanian fens, which are located at the junction of the boreal and continental biogeographical regions
Description of Dyspessa gayane sp. n. collected in southern Armenia with comparison to other relatives from the D. tristis species group (Lepidoptera, Cossidae, Cossinae) /
No full textA new species, Dyspessa gayane sp. n., recently collected in southern Armenia, is described in the present article. A detailed comparison of the new species with other relatives from the Dyspessa tristis A. Bang-Haas, 1912 species group is provided
Hypotrabala lyovushkini sp. n. – an overlooked gem (Lepidoptera, Lasiocampidae, Lasiocampinae, Selenepherini) /
No full textNew Hypotrabala Holland, 1893 species, Hypotrabala lyovushkini sp. n., is described from Kenya. The new species is compared with two sister species: Hypotrabala extenuata Takano & László, 2024 from Guinea and Liberia, and Hypotrabala exquisita Takano & László, 2024 from Congo. Additionally, the former species is recorded for Sierra Leone and Ivory Coast, the former – for the Democratic Republic of the Congo. Adult males, their genitalia, and distribution maps are illustrated
First records of beetle Fauna (Insecta: Coleoptera) from Late Glacial sediments of Lithuania: Novel environmental reconstruction /
No full textThis study presents the first subfossil beetle (Coleoptera) records from Lithuania, from Late Glacial organic deposits. Bulk sediment samples were collected from the Pamerkiai and Zervynos Outcrops in SE Lithuania, and from the Ventė Outcrop at the eastern coast of the Curonian Lagoon, W Lithuania. Radiocarbon dating determined that the studied sediments accumulated between ~15,000–11,300 cal BP. The beetle assemblages (29–177 individuals per sample) consist of many cold-adapted species that are common from Late Glacial deposits in the British Isles, Southern Sweden, and continental Europe. True arctic species are absent from the assemblages, and it is likely that the Lithuanian beetle fauna was most similar to nearby southern regions (e.g., Poland) during the Late Glacial. Besides a variety of aquatic species and typical wetland species, many beetle species living in open environments and on sandy soils were identified. In almost all the samples, taxa associated with pine trees, willows, and birches were found, confirming previous reconstructions of a sparsely forested landscape during the climatic periods GI-1e–GI-1a (Bølling-Allerød). The species assemblages from the youngest samples, associated with GS-1 (Younger Dryas), indicate the disappearance of large aquatic macrophytes and decreasing temperatures in Southern Lithuania, but a persistence of trees in the region