Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Not a member yet
3661 research outputs found
Sort by
Claudia Perandones: “El Malbrán colabora con proyectos de terapias para coronavirus, y en breve habrá resultados interesantes”
No full textInforme de actividades COVID-19
No full textSe detallan las actividades realizadas desde el Instituto Nacional de Producción de Biológicos (INPB) en relación a la epidemia del COVID-19
Científicos argentinos crearon un suero terapéutico anti Covid que logró neutralizar el virus
No full textEs el primer potencial medicamento innovador para el tratamiento de la infección por SARS-CoV-2 totalmente desarrollado en Argentina. Dado el resultado obtenido, se avanzará a la fase de ensayos clínicos en pacientes
Recomendaciones para el manejo de materiales de pacientes en los laboratorios clínicos en tiempos de SARS-COV-2
No full textLa expansión del SARS CoV-2 ha producido un cambio de paradigma en el manejo de las muestras clínicas. Los conocimientos sobre su potencial patógeno y los riesgos de su transmisión son actualmente muy dinámicos.
El objetivo de este documento es proporcionar lineamientos para que los laboratorios puedan discutir y elaborar sus propios algoritmos de trabajo considerando los riesgos que se plantean en consonancia con cada Institución.
Esta orientación provisional se basa en los conocimientos actuales sobre el virus y las recomendaciones de organismos nacionales e internacionales para el manejo de muestras clínicas en general y en particular, para aquellas muestras de pacientes con síntomas de COVID-19.
Estas recomendaciones no pretenden reemplazar las pautas de bioseguridad existentes en cada laboratorio sino (a) reforzar la seguridad del personal de salud que se encuentra desempeñando las actividades de diagnóstico clínico y/o microbiológico, (b) concientizar a los trabajadores de los laboratorios en la implementación de medidas de bioprotección, que tal vez no hayan sido consideradas o abordadas anteriormente y (c) afianzar el entrenamiento del personal del laboratorio en buenas prácticas que permitan mantener una cultura de seguridad laboral
Comparison of DNA extraction methods and real-time PCR assays for the molecular diagnostics of chronic Chagas disease
No full textFil: Ramírez, Juan C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología DR: Mario Fatala Chaben; Argentina.Fil: Silgado, Aroa. Universitat Autònoma de Barcelona, PROSICS. Hospital Universitari Vall d'Hebron. Departament de Microbiologia; Barcelona, España.Fil: Moure, Zaira. Universitat Autònoma de Barcelona, PROSICS. Hospital Universitari Vall d'Hebron. Departament de Microbiologia; España.Fil: de Oliveira, Maykon T. Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Unidade de Cardiologia. Departamento de Clínica Médica; Brasil.Fil: Serre-Delcor, Núria. Universitata Autònoma de Barcelona. Tropical Medicine Unit Vall d´Hebron-Drassanes; Barcelona, España.Fil: Salvador, Fernando. Universitat Autònoma de Barcelona. Department of Infectious Diseases, Vall d'Hebron University Hospital; Barcelona, España.Fil: Oliveira, Inés. Universitata Autònoma de Barcelona. Tropical Medicine Unit Vall d´Hebron-Drassanes; Barcelona, España.Fil: Molina, Israel. Universitat Autònoma de Barcelona. Department of Infectious Diseases, Vall d'Hebron University Hospital; Barcelona, España.Fil: Pumarola, Tomas. Universitat Autònoma de Barcelona, PROSICS. Hospital Universitari Vall d'Hebron. Departament de Microbiologia; España.Fil: Sulleiro, Elena. Universitat Autònoma de Barcelona, PROSICS. Hospital Universitari Vall d'Hebron. Departament de Microbiologia; España.Aim: This work aimed to compare the sensitivity of four protocols for the detection of Trypanosoma cruzi DNA in 98 blood samples from chronic Chagas disease patients. Materials & methods: Two DNA extraction (automated and manual) methods and two T. cruzi satellite DNA qPCRs (with a recent design and the usually used set of primers) were analyzed. Results: Both DNA extraction methods and qPCR assays tested in this work gave comparable qualitative results, although the lowest Ct values were obtained when samples were analyzed using the new set of primers for T. cruzi satellite DNA. Conclusion: Our results encourage the implementation of automated DNA extraction systems and the new T. cruzi qPCR for the molecular diagnostics and treatment response monitoring of chronic Chagas disease patients
Speckle tracking echocardiography in the indeterminate form of Chagas disease
No full textFil: Cianciulli, Tomás Francisco. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Albarracín, Gerardo Ariel. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Napoli Llobera, Mariano. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Prado, Nilda Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Saccheri, María Cristina. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Hernández Vásquez, Yolanda María. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Méndez, Ricardo José. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Beck, Martín Alejandro. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Baez, Karina Giselle. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Fil: Balletti, Lorena Romina. Hospital of the Government of the City of Buenos Aires "Dr. Cosme Argerich". Division of Cardiology. Echocardiography Laboratory; Argentina.Background: Chagas disease is one of the most common diseases in Latin-America, and cardiac involvement is a significant cause of death. Assessment of myocardial strain may detect early myocardial damage.
Objectives: To determine differences in longitudinal strain using speckle tracking to assess regional and global left ventricular function in patients with the indeterminate form of Chagas disease, in comparison with a control group.
Methods: This is a retrospective matched case-control study, conducted in a single center. We evaluated 45 adult patients with Chagas disease, diagnosed with 2 serological methods, without evidence of cardiac involvement, who were compared with 45 healthy control subjects, who were sex- and age-matched. All patients underwent Doppler echocardiography and longitudinal strain with speckle tracking.
Results: Median age was 59 years, and 60% were female. Echocardiographic parameters were similar in patients with Chagas and control subjects. In patients with Chagas, global strain differed significantly from that of control subjects (-17 vs -20.3, P < .001). Segmental strain showed 7 abnormal segments in patients with Chagas (P < .05).
Conclusions: In patients with the indeterminate form of Chagas disease, global and segmental longitudinal peak systolic strain is reduced compared with healthy subjects, thus suggesting that it could be a sensitive technique to detect early myocardial damage. These findings could provide useful information regarding the pathophysiology of cardiac involvement and understand whether they might have prognostic usefulness or help develop strategies to modify the course and prognosis of patients with Chagas disease. A longitudinal prospective study would be necessary to validate our findings
Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012-2018
Full text linkFil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Rapoport, Melina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Albornoz, Ezequiel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Celaya, Federico. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: de Mendieta, Juan. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: de Belder, Denise. National Council on Scientific and Technical Research (CONICET); Argentina.Fil: Lucero, Celeste. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Gomez, Sonia. National Council on Scientific and Technical Research (CONICET); Argentina .Fil: Danze, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.To describe the resistance profile and the genetic characteristics of Escherichia coli isolates that harbor the mobilizable colistin resistance gene mcr-1 in Argentina
Lutzomyia longipalpis, Gone with the Wind and Other Variables
No full textFil: Salomon, O. D. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Medicina Tropical; Puerto Iguazú, Argentina.Lutzomyia longipalpis (Lutz & Neiva) is the main vector of Leishmania infantum (Nicolle) in America, associated in turn with the current spread and urbanization of American visceral leishmaniasis (AVL). The vector distribution in AVL foci shows a spatial-temporal clustering despite the different epidemiological contexts. The factors associated with the macroscale distribution of Lu. longipalpis as a landscape stratification are discussed in the framework of the process of their adaptation to anthropized environments. On the other hand, the fact that Lu. longipalpis is clustered in only a few hot spots or critical sites suggests that microscale approaches that describe the trap surrounding environment and the availability of refuges and food sources are better at explaining the uneven distribution of this vector, and should contribute, together with macroscale variables, to design operational control strategies. With regard to temporal distribution and climatic or vegetation data obtained by remote sensing as variables to explain and forecast the abundance of Lu. longipalpis, it is necessary to take into account the time lags in relation to the life cycle of the vector, the difference between the level of daily activity and actual abundance, and the differences in critical variables and thresholds according to the region or season. In conclusion, this review shows that it is feasible to characterize the distribution of Lu. longipalpis at focus level and within it to identify the main critical sites, proposing a sequential cost-effectivity strategy for urban AVL surveillance and control
Lutzomyia longipalpis, Gone with the Wind and Other Variables
No full textFil: Salomon, O. D. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Medicina Tropical; Puerto Iguazú, Argentina.Lutzomyia longipalpis (Lutz & Neiva) is the main vector of Leishmania infantum (Nicolle) in America, associated in turn with the current spread and urbanization of American visceral leishmaniasis (AVL). The vector distribution in AVL foci shows a spatial-temporal clustering despite the different epidemiological contexts. The factors associated with the macroscale distribution of Lu. longipalpis as a landscape stratification are discussed in the framework of the process of their adaptation to anthropized environments. On the other hand, the fact that Lu. longipalpis is clustered in only a few hot spots or critical sites suggests that microscale approaches that describe the trap surrounding environment and the availability of refuges and food sources are better at explaining the uneven distribution of this vector, and should contribute, together with macroscale variables, to design operational control strategies. With regard to temporal distribution and climatic or vegetation data obtained by remote sensing as variables to explain and forecast the abundance of Lu. longipalpis, it is necessary to take into account the time lags in relation to the life cycle of the vector, the difference between the level of daily activity and actual abundance, and the differences in critical variables and thresholds according to the region or season. In conclusion, this review shows that it is feasible to characterize the distribution of Lu. longipalpis at focus level and within it to identify the main critical sites, proposing a sequential cost-effectivity strategy for urban AVL surveillance and control