Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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Distinct mechanisms of dissemination of NDM-1 metallo- β-lactamase in Acinetobacter spp. in Argentina
No full textA four-year surveillance of carbapenem-resistant Acinetobacter spp. in Argentina identified 40 strains carrying blaNDM-1 Genome sequencing revealed that most were A. baumannii, while seven represented other Acinetobacter spp. The A. baumannii genomes were closely related, suggesting recent spread. blaNDM-1 was located in the chromosome of A. baumannii strains and on a plasmid in non-baumannii strains. A resistance gene island carrying blaPER-7 and other resistance determinants was found on a plasmid in some A. baumannii strains
Reunión con expertos. El Gobierno traza estrategias para la detección, aislamiento, control y atención ante un eventual caso de coronavirus
No full textLuego de analizar la situación con el presidente Alberto Fernández, el ministro de Salud de la Nación, Ginés González García, presidió este martes una reunión con expertos de sociedades científicas para estudiar las últimas novedades sobre el nuevo coronavirus detectado en Wuhan, China, que mantiene en distintos niveles de alerta a la comunidad internacional.
En este marco, junto a los equipos técnicos de la cartera sanitaria, del ANLIS-Malbrán y de la Organización Panamericana de la Salud (OPS), se consensuó la estrategia para la detección precoz, diagnóstico, aislamiento y atención de un posible caso
Reunión con expertos. El Gobierno traza estrategias para la detección, aislamiento, control y atención ante un eventual caso de coronavirus
No full textLuego de analizar la situación con el presidente Alberto Fernández, el ministro de Salud de la Nación, Ginés González García, presidió este martes una reunión con expertos de sociedades científicas para estudiar las últimas novedades sobre el nuevo coronavirus detectado en Wuhan, China, que mantiene en distintos niveles de alerta a la comunidad internacional.
En este marco, junto a los equipos técnicos de la cartera sanitaria, del ANLIS-Malbrán y de la Organización Panamericana de la Salud (OPS), se consensuó la estrategia para la detección precoz, diagnóstico, aislamiento y atención de un posible caso
OMS declara epidemia mundial. Alocución de apertura del Director General de la OMS en la rueda de prensa sobre la COVID-19
No full textAlerta: Incremento y reemergencia de aislamientos de neisseria gonorrhoeae con baja y alta resistencia a la azitromicina en Argentina
No full textEl Programa Nacional de Vigilancia de la Sensibilidad a los Antimicrobianos en Neisseria gonorrhoeae (PROVSAG), dentro del marco de la Red Nacional de Infecciones de Transmisión Sexual, realiza esta vigilancia desde el año 1980 para monitorear la aparición y distribución de aislamientos resistentes en el país y contribuir a las pautas nacionales de tratamiento de la gonorrea.
Como Centro Nacional de Referencia en Enfermedades de Transmisión Sexual y Laboratorio de Referencia para la Gonorrea, es nuestro deber alertar a las autoridades sanitarias y a la comunidad científica del país sobre la emergencia de resistencias que puedan afectar el normal manejo de la gonorrea. Por otra parte, el estudio molecular de los aislamientos involucrados nos permite la identificación de los clones circulantes y la eventual detección de brotes epidémicos.
Este estudio mostró el aumento de aislamientos con baja y alta resistencia a la azitromicina diseminados en seis regiones del país (Córdoba, Buenos Aires, CABA, La Pampa, Río Negro y Neuquén) hasta el año 2019. Además, evidenció la presencia de clones circulantes predominantes (G1 y G2) en estas regiones geográficas.
Estos hallazgos muestran a la provincia de Córdoba como la principal zona geográfica de circulación y expansión de estos aislamientos a nivel nacional. Además, los porcentajes de resistencia a la azitromicina observados en las provincias de Córdoba y Rio Negro (10,8% y 5,4%, respectivamente), llevan a considerar la utilización de azitromicina para el tratamiento de la gonorrea, debido a que supera el valor límite del 5% de resistencia propuesto por la OMS para tratamiento empírico.
Se resalta la importancia del fortalecimiento de la vigilancia de la resistencia a los antimicrobianos en N. gonorrhoeae y la continuación de estudios de genotipificación y epidemiología molecular para evaluar la evolución y/o emergencia de clones circulantes
First insights into the biochemical and toxicological characterization of venom from the Banded Cat-eyed Snake Leptodeira annulata pulchriceps
No full textFil: Sánchez, Matías N. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Medicina Tropical (INMeT); Puerto Iguazú, Argentina.Fil: Gonzalez, Karen. Universidad Nacional del Nordeste (UNNE). Facultad de Ciencias Veterinarias (FCV); Corrientes, Argentina.Fil: Sciani, Juliana M. Universidade São Francisco. Laboratório Multidisciplinar de Pesquisa; San Pablo, Brasil.Fil: Gritti, Micaela Andrea. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Medicina Tropical (INMeT); Puerto Iguazú, Argentina.Fil: Maruñak, Silvana L. Universidad Nacional del Nordeste (UNNE). Facultad de Ciencias Veterinarias (FCV); Corrientes, Argentina.Fil: Tavares, Flávio L. Universidade Federal da Integração Latino-Americana (UNILA); Foz do Iguaçu, Brasil.Fil: Teibler, Gladys P. Universidad Nacional del Nordeste (UNNE). Facultad de Ciencias Veterinarias (FCV); Corrientes, Argentina.Fil: Peichoto, María Elisa. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Medicina Tropical (INMeT); Puerto Iguazú, Argentina.With the aim to widen the current knowledge of toxinological implications of bites from rear-fanged snakes and biological roles of their venoms, this study focuses on the biochemical composition and toxic effects of the venom of Leptodeira annulata pulchriceps from Argentina. We analyzed the protein composition by electrophoresis and mass spectrometry, and enzymatic properties by quantitative assays on different substrates. Additionally, we evaluated local and systemic toxicity in mice, and tested its cross-reactivity with elapid and viperid antivenoms used in Argentina. This venom showed features reminiscent of venoms from snakes of Bothrops genus, containing components ranging from ~17 to 75 kDa, which are mainly tissue-damaging toxins such as proteinases. Although showing low lethality to mice (LD50 = 20 μg/g body weight), prominent hemorrhage developed locally in mice intramuscularly and intradermally injected with the venom, and the minimum hemorrhagic dose was found to be 12.7 μg/mouse. This study is the first comprehensive investigation of the venom of L. a. pulchriceps, and sheds new light on differences between this and those of the other two subspecies of L. annulata. Additionally, the study provides new insights into the venom components of "colubrid" snakes, advocating for considering bites from this rich diversity of snakes as a public health problem that needs to be addressed worldwide
Rapid Diagnostic Tests for Trypanosoma cruzi Infection: Field Evaluation of Two Registered Kits in a Region of Endemicity and a Region of Nonendemicity in Argentina
No full textFil: Lopez-Albizu, Constanza. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Danesi, Emmaría. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación de Endemo-Epidemias; ArgentinaFil: Piorno, Pablo. Asociación para Desarrollo Sanitario Regional (ADESAR); Argentina.Fil: Fernandez, Mariana. Asociación para Desarrollo Sanitario Regional (ADESAR); Argentina.Fil: García Campos, Francisco. Ministerio de Salud Pública de Salta; Argentina.Fil: Scollo, Karenina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Crudo, Favio. Asociación para Desarrollo Sanitario Regional (ADESAR); Argentina.Infection by Trypanosoma cruzi (Chagas disease [ChD]) affects around 7 million people in the Americas, most of whom are unaware of their status due to lack of clinical manifestations and poor access to diagnosis. Rapid diagnostic tests (RDTs) are widely used for screening for different infections (HIV, hepatitis B, and syphilis), and their application for ChD would facilitate access to diagnosis, especially in remote areas where health services have scarce resources. We conducted a prospective intervention study in 2018 to evaluate in the field two in vitro RDTs for ChD, authorized by the National Administration of Medicaments, Aliments, and Medical Technologies of Argentina (ANMAT), in areas of endemicity and nonendemicity in Argentina. We recruited 607 volunteers older than 18 years in Salta province and the city of Buenos Aires. The RDTs Ab Standard Diagnostics SD Bioline (SD) and Check Chagas Wiener Lab (WL) were performed in situ with whole-blood samples, and confirmatory serology was done at a reference center. The rate of infection with T. cruzi was 17.8% (108/607). The SD test showed 97.2% sensitivity (95% confidence interval [CI], 93.5 to 100) and 91.7% specificity (95% CI, 96.2 to 99.2%), and the WL test showed 93.4% sensitivity (95% CI, 88.2 to 98.6%) and 99.1% specificity (95% CI, 91.9 to 100%). The sensitivity and specificity for the two RDTs tested were higher than previously reported. These results encourage the use of the tested RDTs in Salta province and for further field studies for the implementation of these RDTs in other epidemiological scenarios. This will be very important to improve access to diagnosis of Chagas and its clinical management as a neglected disease, especially in remote areas with health access barriers
Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area
Full text linkFil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Sawicki, Mirna. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Microbiología y Parasitología Médica; Argentina.Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud; Argentina.Fil: Velásquez, Jorge Néstor. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina.Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals.
Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp.
Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases.
Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections
Distribution of PCV13 and PPSV23 Streptococcus pneumoniae serotypes in Argentinean adults with invasive disease, 2013-2017
No full textFil: Zintgraff, Jonathan. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Fossati, M. S. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Sorhouet Pereira, Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Veliz, Omar. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Regueira, Mabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Moscoloni, Maria Alicia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Lara, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Napoli, Daniela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Streptococcus pneumoniae is a major cause of severe invasive disease associated with high mortality and morbidity worldwide. To identify the serotypes most commonly associated with infection in adults in Argentina, 791 pneumococcal isolates from 56 hospitals belonging to 16 provinces and Buenos Aires city were serotyped. The isolates were submitted as part of a National Surveillance Program for invasive pneumococcal disease in adults, which started in 2013. Serotypes 3, 8, 12F, 7F and 1 were the most prevalent among adult patients. During the study period there was no significant difference in serotype distribution between the age groups studied (18-64 and ≥65 years old), except for serotype 1, 3 and 23A. Most prevalent serotypes in pneumonia were serotype 7F, 1, 12F, 8, and 3. When the clinical diagnosis was meningitis, serotype 3 and 12F were the most prevalent, whereas when the diagnosis was sepsis/bacteremia the most prevalent was serotype 8. In this work, for the 18-64-year-old group, PPSV23 and PCV13 serotypes accounted for 74.56% and 44.54% respectively of the cases in the studied period. On the other hand, for the ≥65-year-old group, these serotypes represented 72.30% and 41.42% respectively. The aim of this work was to establish the knowledge bases of the serotypes that cause invasive pneumococcal diseases in the adult population in Argentina and to be able to detect changes in their distribution over time in order to explore the potential serotype coverage of the vaccines in current use