Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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Analysis on risk factors associated with birth defects in newborns in the area of the Matanza-Riachuelo river basin
No full textFil: Bidondo, María Paz. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica. Red Nacional de Anomalías Congénitas; Argentina.Fil: Groisman, Boris. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica. Red Nacional de Anomalías Congénitas; Argentina.Fil: Gili, Juan. Centro de Educación Médica e Investigaciones Clínicas. Laboratorio de Epidemiología Genética; Argentina.Fil: Liascovich, Rosa. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica. Red Nacional de Anomalías Congénitas; Argentina.Fil: Martin, María Cecilia. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica. Red Nacional de Anomalías Congénitas; Argentina.Fil: Tocci, A. M. Hospital General de Agudos "Dr. Cosme Argerich"; Buenos Aires, Argentina.Fil: Flores, Romina. Hospital Interzonal General de Agudos "Evita"; Buenos Aires, Argentina.Fil: Pus Barraza, Andrea. Hospital Zonal General de Agudos "Dr. Diego Paroissien"; Buenos Aires, Argentina.Fil: Barbero, Pablo. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica. Red Nacional de Anomalías Congénitas; Argentina.Introducción: en la cuenca Matanza-Riachuelo viven aproximadamente 4.885.000 habitantes y es uno de los sitios más contaminados de Argentina. Este estudio evaluó factores de riesgo asociados a anomalías congénitas (AC) y bajo peso al nacimiento. Métodos: se realizó un estudio caso-control en tres hospitales de la cuenca pertenecientes la Red Nacional de Anomalías Congénitas (RENAC) durante un año. Se evaluó asociación de bajo peso y AC, con variables socioeconómicas; reproductivas; distancia de la vivienda durante la gestación a industrias y a cursos de agua. Resultados: la prevalencia de anomalías congénitas en recién nacidos fue de 1,93% (IC 95%: 1,64 - 2,25). Los factores de riesgo asociados a AC fueron enfermedades maternas agudas y consumo de medicamentos. Los casos presentaron peso y edad gestacional significativamente más bajos y mayor edad materna. No se encontró asociación entre bajo peso o prevalencia de AC con distancia de la vivienda durante el embarazo a industrias ni a los cursos de agua. Conclusión principal: este estudio es la primera investigación en el área de las AC con datos georreferenciados en nuestro país y ha mostrado la factibilidad de efectuar este tipo de investigaciones para evaluar factores de riesgo en la RENAC. Futuras investigaciones orientadas a evaluar AC específicas deberían realizarse a fin de corroborar los resultados del presente estudio
Genetic Imbalances in Argentinean Patients with Congenital Conotruncal Heart Defects
Get PDFFil: Delea, Marisol. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Espeche, Lucía D. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Bruque, Carlos D. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Bidondo, María Paz. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Massara, Lucía S. Hospital El Cruce Dr. Néstor Kirchner; Argentina.Fil: Oliveri, Jaen. Hospital El Cruce Dr. Néstor Kirchner; Argentina.Fil: Brun, Paloma. Hospital El Cruce Dr. Néstor Kirchner; Argentina.Fil: Cosentino, Viviana R. Hospital Gandulfo. Departamento de Neonatología; Argentina.Fil: Martinoli, Celeste. Hospital Sor María Ludovica. Servicio de Genética; Argentina.Fil: Tolaba, Norma. Hospital Dr. Arturo Oñativia; Argentina.Fil: Picon, Claudina. Hospital Pediátrico Dr. Avelino Castelán; Argentina.Fil: Ponce Zaldua, María Eugenia. Hospital Provincial Neuquén Dr. Eduardo Castro Rendón. Servicio de Genética; Argentina.Fil: Ávila, Silvia. Hospital Provincial Neuquén Dr. Eduardo Castro Rendón. Servicio de Genética; Argentina.Fil: Gutnisky, Viviana. Laboratorio Central de Redes y Programas de Corrientes; Argentina.Fil: Pérez, Myriam. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Furforo, Lilian. Hospital Materno Infantil Dr. Ramón Sardá; Argentina.Fil: Buzzalino, Noemí D. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Liascovich, Rosa. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Groisman, Boris. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Rittler, Mónica. Hospital Materno Infantil Dr. Ramón Sardá; Argentina.Fil: Rozental, Sandra. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Barbero, Pablo. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Fil: Dain, Liliana. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.Congenital conotruncal heart defects (CCHD) are a subset of serious congenital heart defects (CHD) of the cardiac outflow tracts or great arteries. Its frequency is estimated in 1/1000 live births, accounting for approximately 10⁻30% of all CHD cases. Chromosomal abnormalities and copy number variants (CNVs) contribute to the disease risk in patients with syndromic and/or non-syndromic forms. Although largely studied in several populations, their frequencies are barely reported for Latin American countries. The aim of this study was to analyze chromosomal abnormalities, 22q11 deletions, and other genomic imbalances in a group of Argentinean patients with CCHD of unknown etiology. A cohort of 219 patients with isolated CCHD or associated with other major anomalies were referred from different provinces of Argentina. Cytogenetic studies, Multiplex-Ligation-Probe-Amplification (MLPA) and fluorescent in situ hybridization (FISH) analysis were performed. No cytogenetic abnormalities were found. 22q11 deletion was found in 23.5% of the patients from our cohort, 66% only had CHD with no other major anomalies. None of the patients with transposition of the great vessels (TGV) carried the 22q11 deletion. Other 4 clinically relevant CNVs were also observed: a distal low copy repeat (LCR)D-E 22q11 duplication, and 17p13.3, 4q35 and TBX1 deletions. In summary, 25.8% of CCHD patients presented imbalances associated with the disease
Bactericidal and Cytotoxic Activities of Polyphenol Extracts from Solanum tuberosum spp. tuberosum and spp. andigena Cultivars on Escherichia coli and Human Neuroblastoma SH-SY5Y Cells In Vitro
No full textFil: Silveyra, María Ximena. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina.Fil: Lanteri, María Luciana. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina.Fil: Damiano, Rocío Belén. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología; Argentina.Fil: Andreu, Adriana Balbina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina.Potatoes (Solanum tuberosum L.) are a good source of dietary antioxidant polyphenols. This study investigated the potential antioxidant, bactericidal, and cytotoxic activities in vitro of the phenolic compounds present in tubers of one S. tuberosum spp. tuberosum (Summerside), and three S. tuberosum spp. andigena (landraces Moradita, Waicha, and Santa María) cultivars. Both the content of phenolic acids, chlorogenic acids (CGAs) being the most abundant, and the antioxidant activity were higher in extracts from skin than from flesh. Extracts from Moradita flesh and Summerside skin showed bactericidal activity against Escherichia coli ATCC 25922 but failed to inhibit pathogenic E. coli O157. Both extracts lack pigmentation but do contain 5-CGA, caffeic, and ferulic acids. Positive control with gentamicin and commercial 5-CGA resulted in a complete inhibition of bacterial growth. In addition, all potato extracts and commercial 5-CGA diminished dose-dependently human neuroblastoma SH-SY5Y cell viability. Skin extracts were more potent than flesh extracts. Among flesh extracts, Moradita was the most potent. Together, our results suggest that Moradita flesh could provide a desirable source of important health-promoting substances. Findings indicate that the biological activity of potato extracts is a combination of various bioactive compounds and contribute to the revalorization of potato as a functional food
Distribution of influenza virus types by age using case-based global surveillance data from twenty-nine countries, 1999-2014
Get PDFInfluenza disease burden varies by age and this has important public health implications. We compared the proportional distribution of different influenza virus types within age strata using surveillance data from twenty-nine countries during 1999-2014 (N=358,796 influenza cases)
Primeiro relato da presença de Leptospira interrogans em roedores sigmodontíneos Scapteromys aquaticus
No full textLeptospirosis is a globally distributed zoonosis that can be transmitted through direct or indirect contact with the urine or tissues of infected animals. In Argentina, leptospirosis is endemic in the province of Santa Fe and epidemic outbreaks occur during floods. However, very little is known about the role that wild rodents play in the spread of the disease in Argentina. The objective of this study was to identify the host species of pathogenic Leptospira among rodents in a riverine settlement in the province of Santa Fe.We conducted a trapping session in October 2015. Kidneys of the captured animals were analyzed by real-time PCR for the LipL32 gene of pathogenic Leptospira. Animals that were positive were subjected to microscopic agglutination test (MAT) and molecular typing by amplification of the 16S rRNA gene and two multilocus sequence typing (MLST) schemes.A total of 37 rodents of the species Akodon azarae, Cavia aperea, Oligoryzomys flavescens, Rattus rattus, and Scapteromys aquaticus were captured. Real-time PCR found one male Scapteromys aquaticus that was positive. The serum of this individual and of the rest of the S. aquaticus captured (n = 18) were analyzed by MAT and were non-reactive for the 10 serovars tested. Amplification of the 16S rRNA gene identified the infective species as Leptospira interrogans, while amplification could not be obtained for the two MLST schemes.The findings of this study contribute new information concerning the presence of pathogenic Leptospira in wild rodents, which is relevant in this region because the species is widely distributed in swampy and flood-prone environments of South America
Anti-inflammatory agents reduce microglial response, demyelinating process and neuronal toxin uptake in a model of encephalopathy produced by Shiga Toxin 2
No full textFil: Pinto, Alipio. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica "Houssay". Laboratorio de Neurofisiopatología; Argentina.Fil: Berdasco, Clara. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica "Houssay". Laboratorio de Neurofisiopatología; Argentina.Fil: Arenas-Mosquera, David. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica "Houssay". Laboratorio de Neurofisiopatología; Argentina.Fil: Cangelosi, Adriana. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina.Fil: Geoghegan, Patricia A. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos (CNCCB); Argentina.Fil: Nuñez, Myriam C. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Matemáticas; Argentina.Fil: Goldstein, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica "Houssay". Laboratorio de Neurofisiopatología; Argentina.Infections by Enterohemorrhagic Escherichia coli may cause in addition to hemolytic uremic syndrome neurological disorders which may lead to fatal outcomes in patients. The brain striatum is usually affected during this outcome. The aim of this study was to determine in this area the role of the microglia in pro-inflammatory events that may occur during Shiga toxin 2 intoxication and consequently to this, whether oligodendrocytes were being affected. In the present paper we demonstrated that anti-inflammatory treatments reduced deleterious effects in brain striatal cells exposed to Shiga toxin 2 and LPS. While dexamethasone treatment decreased microglial activation and recovered myelin integrity in the mice striatum, etanercept treatment decreased neuronal uptake of Stx2 in rat striatal neurons, improving the affected area from toxin-derived injury. In conclusion, microglial activation is related to pro-inflammatory events that may deteriorate the brain function during intoxication with Stx2 and LPS. Consequently, the role of anti-inflammatory agents in the treatment of EHEC-derived encephalopathy should be studied in clinical trials
Prevalence of Pertussis Antibodies in Maternal Blood, Cord Serum, and Infants From Mothers With and Those Without Tdap Booster Vaccination During Pregnancy in Argentina
Get PDFFil: Fallo, Aurelia A. Department of Pediatric Infectious Diseases, School of Medicine, Hospital de Niños "Ricardo Gutiérrez," University of Buenos Aires; Argentina.Fil: Neyro, Silvina E. Department of Pediatric Infectious Diseases, School of Medicine, Hospital de Niños "Ricardo Gutiérrez," University of Buenos Aires; Argentina.Fil: Manonelles, Gabriela V. Department of Pediatric Infectious Diseases, School of Medicine, Hospital de Niños "Ricardo Gutiérrez," University of Buenos Aires; Argentina.Fil: Lara, Claudia. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS); Argentina.Fil: Hozbor, Daniela. Laboratorio VacSal, Instituto de Biotecnología y Biología Molecular (IBBM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT-CONICET; Argentina.Fil: Zintgraff, Jonathan. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS); Argentina.Fil: Mazzeo, Silvina. Departments of Obstetrics and Gynecology, Hospital D. F. Santojanni, Buenos Aires; Argentina.Fil: Davison, Héctor E. Departments of Obstetrics and Gynecology, Hospital D. F. Santojanni, Buenos Aires; Argentina.Fil: González, Susana. Pediatrics, Hospital D. F. Santojanni, Buenos Aires; Argentina.Fil: Zapulla, Estella. Pediatrics, Hospital D. F. Santojanni, Buenos Aires; ArgentinaFil: Canle, Oscar. Blood Center, Hospital de Niños "Ricardo Gutiérrez," Buenos Aires; ArgentinaFil: Huespe, Miguel. Departments of Obstetrics and Gynecology, Hospital D. F. Santojanni, Buenos Aires; Argentina.Fil: Galas, Marcelo. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS); Argentina.Fil: López, Eduardo L. Department of Pediatric Infectious Diseases, School of Medicine, Hospital de Niños "Ricardo Gutiérrez," University of Buenos Aires; Argentina.Morbidity and mortality rates for pertussis in infants are high because disease often occurs before the onset of routine immunization or in those who do not complete a primary immunization series. Pertussis immunization is recommended during pregnancy to achieve antibody levels sufficient to protect young infants. To our knowledge, no previous reports of maternal pertussis immunization results in Latin America exist in the literature
Distinct monocyte subset phenotypes in patients with different clinical forms of chronic Chagas disease and seronegative dilated cardiomyopathy
No full textFil: Pérez-Mazliah, Damián E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Castro Eiro, Melisa D. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Álvarez, María Gabriela. Hospital Interzonal General de Agudos Eva Perón; Argentina.Fil: Lococo, Bruno. Hospital Interzonal General de Agudos Eva Perón; Argentina.Fil: Bertocchi, Graciela. Hospital Interzonal General de Agudos Eva Perón; Argentina.Fil: César, Gonzalo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Natale, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Albareda, María C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Viotti, Rodolfo. Hospital Interzonal General de Agudos Eva Perón; Argentina.Fil: Laucella, Susana A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Chronic infection with Trypanosoma cruzi leads to a constant stimulation of the host immune system. Monocytes, which are recruited in response to inflammatory signals, are divided into classical CD14hiCD16-, non-classical CD14loCD16+ and intermediate CD14hiCD16+ subsets. In this study, we evaluated the frequencies of monocyte subsets in the different clinical stages of chronic Chagas disease in comparison with the monocyte profile of seronegative heart failure subjects and seronegative healthy controls. The effect of the anti-parasite drug therapy benznidazole on monocyte subsets was also explored
A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage
No full textFil: Casani, Lucia. Universidad Nacional de Mar del Plata. Facultad de ingeniería. Grupo de Investigación de Ingeniería de Alimentos; Argentina.Fil: Santos, Mauricio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Gerbino, Esteban. Consejo Nacional de Investigaciones Argentinas (CONICET); Argentina.Fil: Moreira, María del Rosario. Universidad Nacional de Mar del Plata. Facultad de ingeniería. Grupo de Investigación de Ingeniería de Alimentos; Argentina.Fil: Gómez-Zavaglia, Andrea. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA, CCT-CONICET); Buenos Aires, Argentina.In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound). The main spectral differences were observed in the 1200 to 900 cm-1 region. The presence of FOS was identified by the typical bands at 1134, 1034, and 935 cm-1 . During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC). A principal component analysis was performed on the FTIR spectra corresponding to the different treatments. The groups observed explained more than 94% of the variance and were related to changes in the carbohydrate composition during storage. Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5. The carbohydrates' concentrations determined by HPLC were used as reference method. The models were validated with independent sets of data. The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples. In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration
A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
No full textFil: de Roodt, Adolfo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Fernández, Julián. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; San José, Costa Rica.Fil: Solano, Daniela. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; San José, Costa Rica.Fil: Lomonte, Bruno. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; San José, Costa Rica.Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of Misiones has received little attention. While exploring the chromatographic venom profile of Argentinean B. cotiara, a major protein peak was found which, according to a previous study, is not present in the venom of Brazilian origin. The corresponding protein was isolated by RP-HPLC, and characterized by electrophoresis, mass spectrometry, phospholipase A2 (PLA2) assay, and myotoxic activities. Representing nearly 15% of B. cotiara venom from Misiones, this protein was identified as a Lys49 PLA2 homologue. In accordance with the characteristics of this toxin family, the protein induced myotoxicity in mice and was devoid of PLA2 activity. Since previous work reported that no PLA2 or PLA2-homologues occur in B. cotiara venom of Brazilian origin, the presence of an abundant Lys49 PLA2 homologue in the venom from Misiones highlights a striking phenotypic variation in toxin expression within two populations of a single snake species inhabiting different geographic areas. The considerable proportion of B. cotiara Lys49 PLA2 homologue myotoxin in the venom alerts that skeletal muscle necrosis might be a potentially relevant consequence of eventual envenomings by this species in Misiones