Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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First detection of Cryptosporidium DNA in blood and cerebrospinal fluid of HIV-infected patients
Get PDFFil: Velásquez, Jorge Néstor. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.Fil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Vittar, Natalia. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.Fil: Nigro, Mónica Gabriela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Figueiras, Olga. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.Fil: Astudillo, Osvaldo Germán. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Ricart, Javier. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.Fil: Della Paolera, Daniela. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.Fil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Human cryptosporidiosis is an intestinal infection caused by different species belonging to the genus Cryptosporidium in both immunocompetent and immunocompromised individuals. The life cycle of Cryptosporidium sp. when affecting the digestive system is well known but the infection of other organs is less studied. Molecular methods are necessary for species and subtypes identification. The goal of this work is to propose a new approach that contributes to the diagnosis of the extra-intestinal dissemination process of Cryptosporidium infection. Cryptosporidium sp. was detected in stool and biopsy samples of two HIV-infected patients. DNA was extracted from feces, biopsy specimens, blood, and cerebrospinal fluid (CSF). All samples were analyzed by nested PCR-RFLP of the 18S rDNA, real-time PCR, and gp60 subtyping. Cryptosporidium DNA was detected in stool and tissue samples and it was also present in blood and CSF samples. Both cases were characterized as Cryptosporidium hominis subtype IeA11G3T3. This is the first report that demonstrates the presence of Cryptosporidium DNA in blood and CSF of HIV-infected patients
Eficacia de la amplificación de la polimerasa con recombinasa para diagnosticar la infección por Trypanosoma cruzi en perros con alteraciones cardíacas en un área endémica de México.
No full textChagas disease is a lingering Public Health problem in Latin America with ∼5.7 million people infected with Trypanosoma cruzi. Transmission is still taking place in most countries of the Americas, including the United States. Dogs are frequently infected with T. cruzi and its high infection prevalence is associated with increased risk of Chagas disease in humans. The city of Mérida in the Yucatan peninsula is endemic for Chagas disease and canines are frequently infected with T. cruzi. The objective of this study was to evaluate the performance of a qualitative point of care (POC) molecular test (RPA-LF, recombinase polymerase amplification-lateral flow) developed in our laboratory for identifying infected dogs. We used retrospective samples of dogs that came for consultation because of cardiac alterations and proved to be infected with T. cruzi as determined by enzyme-linked immunosorbent assay (ELISA), Western blot, and quantitative PCR (qPCR). The analytical sensitivity indicated that RPA-LF amplified T. cruzi DNA in samples containing almost equal to one to two parasites per reaction. Serial twofold dilutions of T. cruzi epimastigotes showed that the test had 95% (19/20) repeatability at concentrations of two parasites per reaction. The test showed no cross reactivity with human DNA or other protozoan parasites (Trypanosoma rangeli, Leishmania spp., and Plasmodium spp.). RPA-LF had the capacity to amplify all discrete typing units (DTUs I-VI) of T. cruzi that circulate in domestic or extradomestic environments. The RPA-LF had 93.2% (95% confidence interval 87.2-98.1) sensitivity and excellent agreement with qPCR used as gold standard (Cohen's Kappa test = 0.963). ELISA was positive in 96.6% (85/88) of dogs, which together with the molecular tests confirmed the frequent contact with infected triatomine bugs in the city of Mérida. These preliminary results on the diagnostic efficacy of the RPA-LF deserve further large-scale field testing of this POC test for T. cruzi infection in endemic areas
Prevalence and Features of Asthma in Young Adults in Urban Areas of Argentina
No full textFil: Arias, Sergio J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Neffen, Hugo. Centro de Alergia, Inmunología y Enfermedades Respiratorias, Santa Fe; Argentina.Fil: Bossio, Juan Carlos. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Calabrese, Carina A. Ministerio de Salud de la Nación Argentina. Dirección de Promoción de la Salud y Control de Enfermedades No Transmisibles. Programa Nacional de Prevención y Control de Enfermedades Respiratorias Crónicas; Argentina.Fil: Videla, Alejandro J. Ministerio de Salud de la Nación Argentina. Dirección de Promoción de la Salud y Control de Enfermedades No Transmisibles. Programa Nacional de Prevención y Control de Enfermedades Respiratorias Crónicas; ArgentinaFil: Armando, Gustavo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Antó, Joseph M. Instituto de Salud Global de Barcelona (ISGlobal), Centre for Research in Environmental Epidemiology (CREAL), Barcelona; España.Objetivo
Estimar la carga del asma en la población adulta joven en zonas urbanas de Argentina.
Diseño
Se realizó una encuesta telefónica a nivel nacional en sujetos de 20 a 44 años en áreas urbanas de Argentina. Se utilizó el cuestionario de la European Community Respiratory Health Survey. El asma se definió como ataque en el último año o uso de medicamentos para el asma.
Resultados
Mil quinientos veintiún sujetos fueron encuestados (62,4% mujeres, edad media 33 años). Noventa y uno fueron clasificados como asmáticos (5,9%, IC 95% 4,7-7,1). La prevalencia ajustada por edad, sexo y nivel educativo fue de 6,4% (IC 95% 5,1-7,7). El porcentaje de sibilancia fue de un 13,9% (IC 95% 15,6-12,2) y el de diagnóstico médico de asma, de un 9,5% (IC 95% 8,0-11,0). Entre los diagnósticos (n = 154), el 71,3% realizó una espirometría y el 51,9% de los tratados (n = 77) utilizaba medicamentos diariamente y un 46,8% como rescate. De los que reportaron algún ataque en el último año (n = 60), un 55% tuvieron una visita a Urgencias y un 23,3% fueron hospitalizados. El asma se asoció con rinitis (OR 11,1; IC 95% 6,2-19,9) e historia familiar (OR 3,6; IC 95% 2,3-5,5).
Conclusión
La prevalencia de asma en adultos jóvenes en Argentina es similar a la de Europa. Aunque los ataques y las admisiones fueron comunes, el uso regular de los medicamentos fue informado por la mitad de los tratados. Los resultados pueden ser útiles para otros países de América Latina.
(EN) Objective
To investigate the burden of asthma in a young adult population in urban areas of Argentina.
Design
A nationwide telephone survey in subjects aged 20-44 years was performed in urban areas in Argentina. The European Community Respiratory Health Survey questionnaire was used. Asthma was defined as an exacerbation in the last year or use of asthma medications.
Results
In total, 1,521 subjects responded (62.4% females, mean age 33 years), of whom 91 were classified as asthmatics (5.9%, 95% CI 4.7-7.1). Prevalence adjusted for age, sex and education level was 6.4% (95% CI 5.1-7.7). Wheezing was reported by 13.9% (95% CI 15.6-12.2) and a diagnosis of asthma by 9.5% (95% CI 8.0-11.0). Among individuals with a diagnosis of asthma (n = 154), 71.3% had undergone spirometry. Among those treated (n = 77), 51.9% used medications daily and 46.8% as a rescue measure. Of those reporting an exacerbation in the last year (n = 60), 55% had attended an emergency department and 23% were admitted. Asthma was associated with rhinitis (OR 11.1, 95% CI 6.2-19.9) and family history (OR 3.6, 95% CI 2.3-5.5).
Conclusion
Asthma prevalence in young adults in Argentina is similar to Europe. Although attacks and admissions were common, regular use of medications was reported by half of those treated. These results may be useful for other Latin American countries
Trypanosoma cruzi serinecarboxipeptidase is a sulfated glycoprotein and a minor antigen in human Chagas disease infection
No full textFil: Soprano, Luciana L ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Parente, Juliana E. Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Departamento de Química Orgánica-Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires; Argentina.Fil: Landoni, Malena. Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Departamento de Química Orgánica-Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires; Argentina.Fil: Couto, Alicia S. Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Departamento de Química Orgánica-Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires; Argentina.Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.In this work, the presence of sulfated N-glycans was studied in a high-mannose-type glycoprotein of Trypanosoma cruzi with serinecarboxipeptidase (TcSCP) activity. The immune cross-reactivity between purified SCP and Cruzipain (Cz) was evidenced using rabbit sera specific for both glycoproteins. Taking advantage that SCP co-purifies with Cz from Concanavalin-A affinity columns, the Cz-SCP mixture was desulfated, ascribing the cross-reactivity to the presence of sulfate groups in both molecules. Therefore, knowing that Cz is a sulfated glycoprotein, with antigenic sulfated epitopes (sulfotopes), SCP was excised from SDS-PAGE and the N-glycosydic chains were analyzed by UV-MALDI-TOF-MS, confirming the presence of short-sulfated high-mannose-type oligosaccharidic chains. Besides, the presence of sulfotopes was analyzed in lysates of the different parasite stages demonstrating that a band with apparent molecular weight similar to SCP was highly recognized in trypomastigotes. In addition, SCP was confronted with sera of infected people with different degrees of cardiac dysfunction. Although most sera recognized it in different groups, no statistical association was found between sera antibodies specific for SCP and the severity of the disease. In summary, our findings demonstrate (1) the presence of sulfate groups in the N-glycosidic short chains of native TcSCP, (2) the existence of immune cross-reactivity between Cz and SCP, purified from epimastigotes, (3) the presence of common sulfotopes between both parasite glycoproteins, and (4) the enhanced presence of sulfotopes in trypomastigotes, probably involved in parasite-host relationship and/or infection. Interestingly, we show for the first time that SCP is a minor antigen recognized by most of chronic Chagas disease patient's sera
A Combined Approach of Infrared Spectroscopy and Multivariate Analysis for the Simultaneous Determination of Sugars and Fructans in Strawberry Juices During Storage
No full textFil: Casani, Lucia. Universidad Nacional de Mar del Plata. Facultad de ingeniería. Grupo de Investigación de Ingeniería de Alimentos; Argentina.Fil: Santos, Mauricio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Gerbino, Esteban. Consejo Nacional de Investigaciones Argentinas (CONICET); Argentina.Fil: Moreira, María del Rosario. Universidad Nacional de Mar del Plata. Facultad de ingeniería. Grupo de Investigación de Ingeniería de Alimentos; Argentina.Fil: Gómez-Zavaglia, Andrea. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA, CCT-CONICET); Buenos Aires, Argentina.In this work, a Fourier transform mid-infrared spectroscopy (FTIR)-based method was developed for simultaneously quantifying simple sugars and exogenously added fructooligosaccharides (FOS) in strawberry juices preserved for up to 14 d using nonthermal techniques (geraniol and vanillin+ultrasound). The main spectral differences were observed in the 1200 to 900 cm-1 region. The presence of FOS was identified by the typical bands at 1134, 1034, and 935 cm-1 . During storage, a significant decrease of sucrose was concomitant to an increase of glucose and fructose in juices stored without any previous preservation treatment, as determined by high-performance liquid chromatography (HPLC). A principal component analysis was performed on the FTIR spectra corresponding to the different treatments. The groups observed explained more than 94% of the variance and were related to changes in the carbohydrate composition during storage. Then, different partial least square models (PLS) were defined to determine the concentrations of glucose, sucrose, fructose, and those of exogenously added FOS with degrees of polymerization within 3 and 5. The carbohydrates' concentrations determined by HPLC were used as reference method. The models were validated with independent sets of data. The mean of predicted values fitted nicely those obtained by HPLC (correlation and R2 > 0.97), thus supporting the use of the PLS models to monitor the quality of strawberry juices in unknown samples. In conclusion, FTIR spectroscopy appears as an adequate analytical tool to quick assess whether juice formulations meet specifications in terms of authenticity, contamination and/or deterioration
Natural Snake Venom Inhibitors and their Pharmaceutical Uses: Challenges and Possibilities
No full textFil: Saavedra, Soledad Lorena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Ávila, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Giudicessi, Silvana Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Albericio, Fernando. University of Barcelona. Department of Organic Chemistry; España.Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Martinez-Ceron, Maria Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Nowadays, treatment with specific antivenins is considered the only cure for snakebites accidents. However, access to antivenom obstructs the successful implementation of the World Health Organization international guidelines. In the last few years, natural organic compounds, peptides, and proteins with the ability to inhibit snake toxins and obtained from different sources such as plant extracts and animal blood have been proposed as antivenoms. In this work, we will focus on the inhibitors of the main venom toxins, phospholipases A2 and metalloproteinases, and their application as novel antivenoms
Autochthonous case of spotted fever caused by Rickettsia parkeri in Ensenada, Buenos Aires
No full textFil: Villalba, Pedro. Hospital Escuela de Agudos "Dr. Ramón Madariaga". Servicio de Infectología; Misiones, Argentina.Fil: Nava, Santiago. Estación Experimental Agropecuaria Rafaela. Instituto Nacional de Tecnología Agropecuaria. Laboratorio de Parasitología e Inmunología; Santa Fe, Argentina.Fil: Brignone, Julia. ANLIS. Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas. Departamento Diagnóstico Laboratorial y Referencial; Argentina.Fil: Sen, Carina. ANLIS. Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas. Departamento Diagnóstico Laboratorial y Referencial; Argentina.Fil: Esposto, Amadeo. Hospital Interzonal General de Agudos General San Martin. Servicio de Infectologia; Buenos Aires, Argentina.Fil: Angeletti, Virginia. Hospital Interzonal General de Agudos General San Martin. Servicio de Infectologia; Buenos Aires, Argentina.Se comunica un caso autóctono de fiebre manchada por Rickettsia parkeri en un adulto residente en Ensenada, Provincia de Buenos Aires ocurrido en el verano de 2016. El cuadro, secundario a una mordedura de garrapata en la pierna izquierda, se presentó como un síndrome febril agudo con deterioro del estado general, cefalea, mialgias, artralgias y exantema maculopapular. El sitio de la mordedura presentaba una úlcera con escara necrótica. El diagnóstico se confirmó por conversión serológica IgG anti-antígenos del género Rikettsia. La secuencia de un fragmento del gen gltA amplificado a partir de la lesión de piel presentó 100% identidad nucleotídica con las secuencias de cepas de R. parkeri aisladas en Argentina y en varios países de América. El paciente evolucionó favorablemente al tratamiento con doxiciclina
Toxocariasis in Carnivora from Argentinean Patagonia: Species molecular identification, hosts, and geographical distribution
No full textFil: Vega, R M. Laboratorio de Parasitología, INIBIOMA (CONICET-UNCo), Quintral 1250, 8400, Bariloche, Río Negro; Argentina.Fil: Gonzalez Prous, C L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Krivokapich, Silvio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Gatti, Graciana Mabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Brugni, N L. Laboratorio de Parasitología, INIBIOMA (CONICET-UNCo), Quintral 1250, 8400, Bariloche, Río Negro; Argentina.Fil: Semenas, L. Laboratorio de Parasitología, INIBIOMA (CONICET-UNCo), Quintral 1250, 8400, Bariloche, Río Negro; Argentina.Twenty four specimens of seven species belonging to the families Felidae, Mustelidae, and Canidae were obtained in Lanín and Nahuel Huapi National Parks from March 1996 to April 2016. Specimens were processed by necropsy in order to contribute to the knowledge of toxocariasis in wild carnivores of Argentinean Patagonia. The only Puma concolor and the seven Leopardus geoffroyi were positive for Toxocara cati. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the ITS-1 region from larval and adult DNA was carried out to confirm parasite species identification. This is the first molecular determination of T. cati from wild felids in Argentina and the study also fill gaps about the spatial distribution and hosts for Toxocara cati
Successful management with fosfomycin + ceftazidime of an infection caused by multiple highly-related subtypes of multidrug-resistant and extensively drug-resistant KPC-producing Serratia marcescens
Get PDFFil: Rodriguez, Cecilia. Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas (CERELA-CONICET), San Miguel de Tucumán, Tucumán; Argentina.Fil: Brengi, Silvina P. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Enterobacterias; Argentina.Fil: Cáceres, Mariel Agustina. Laboratorio de Bacteriología del Hospital 'Ángel C. Padilla', San Miguel de Tucumán, Tucumán; Argentina.Fil: Mochi, Silvana. Laboratorio de Bacteriología del Hospital 'Ángel C. Padilla', San Miguel de Tucumán, Tucumán; Argentina.Fil: Viñas, María R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Enterobacterias; Argentina.Fil: Rizza, Constanza Antich. Laboratorio de Microbiología del Hospital del Niño Jesús, San Miguel de Tucumán, Tucumán; Argentina.Fil: Merletti, G. Laboratorio de Microbiología del Hospital del Niño Jesús, San Miguel de Tucumán, Tucumán; Argentina.Fil: Bru, Elena. Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas (CERELA-CONICET), San Miguel de Tucumán, Tucumán; Argentina.Fil: Assa, José Daniel. Laboratorio de Microbiología del Hospital del Niño Jesús, San Miguel de Tucumán, Tucumán; Argentina.Fil: Raya, Raúl Ricardo. Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas (CERELA-CONICET), San Miguel de Tucumán, Tucumán; Argentina.Fil: Centrón, Daniela. Instituto de Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires (IMPaM, UBA-CONICET), Buenos Aires, Argentina
Posaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of cyp51A Mutations on Estimation of Epidemiological Cutoff Values
Get PDFFil: Espinel-Ingroff, A. VCU Medical Center, Richmond, Virginia; Estados Unidos.Fil: Turnidge, J. University of Adelaide; Australia.Fil: Alastruey-Izquierdo, A. Instituto de Salud Carlos III. National Centre for Microbiology. Mycology Reference Laboratory; España.Fil: Dannaoui, E. Université Paris-Descartes. Faculté de Médecine. APHP. Service de Microbiologie. Unité de Parasitologie-Mycologie. Hôpital Européen Georges Pompidou; Francia.Fil: Garcia-Effron, Guillermo. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET). Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Micología y Diagnóstico Molecular; Argentina.Fil: Guinea, J. Instituto de Investigación Sanitaria Gregorio Marañón. Hospital General Universitario Gregorio Marañon. Servicio de Microbiología Clínica y Enfermedades Infecciosas-VIH; España.Fil: Kidd, S. National Mycology Reference Centre. Microbiology & Infectious Diseases. SA Pathology; Australia.Fil: Pelaez, T. Hospital Universitario Central de Asturias. Servicio de Microbiología; España.Fil: Sanguinetti, M. Università Cattolica del Sacro Cuore. Institute of Microbiology, Roma; Italia.Fil: Meletiadis, J. National and Kapodistrian University of Athens. Medical School. Attikon Hospital. Clinical Microbiology Laboratory; Grecia.Fil: Botterel, F. CHU Henri Mondor. DHU VIC. Parasitologie-Mycologie. Bactériologie-Hygiène. Département de Virologie Créteil. Unité de Parasitologie-Mycologie; Francia.Fil: Bustamante, B. Universidad Peruana Cayetano Heredia. Instituto de Medicina Tropical Alexander von Humboldt; Perú.Fil: Chen, Y-C. National Taiwan University Hospital. College of Medicine. Department of Internal Medicine; Taiwan.Fil: Chakrabarti, A. Postgraduate Institute of Medical Education & Research. Department of Medical Microbiology; India.Fil: Chowdhary, A. University of Delhi. Vallabhbhai Patel Chest Institute. Department of Medical Mycology; India.Fil: Chryssanthou, E. Universitetlaboratoriet. Klinisk Mikrobiologi. Universitetssjukhuset; Suecia.Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Gonzalez, G. M. Universidad Autónoma de Nuevo León, Monterrey, Nuevo León; México.Fil: Guarro, J. Universitat Rovira i Virgili. Mycology Unit Medical School; España.Fil: Johnson, E. M. Public Health England. Mycology Reference Laboratory, Bristol; Inglaterra.Fil: Kus, J. V. Public Health Ontario; Canadá.Fil: Lass-Flörl, C. Medical University of Innsbruck. Division of Hygiene and Medical Microbiology. National Mycology Reference Centre; Austria.Fil: Linares-Sicilia, M. J. H. G. U. Reina Sofía. Universidad de Córdoba; España.Fil: Martín-Mazuelos, E. Hospital de Valme. Unidad de Gestión Clínica de Enfermedades Infecciosas y Microbiología, Sevilla; España.Fil: Negri, C. E. Universidade Federal de São Paulo. Laboratório Especial de Micologia, São Paulo, Brasil.Fil: Pfaller, M. A. University of Iowa College of Medicine, Iowa City, Iowa; Estados Unidos.Fil: Tortorano, A. M. Università degli Studi di Milano. Department of Biomedical Sciences for Health; Italia.Estimating epidemiological cutoff endpoints (ECVs/ECOFFS) may be hindered by the overlap of MICs for mutant and nonmutant strains (strains harboring or not harboring mutations, respectively). Posaconazole MIC distributions for the Aspergillus fumigatus species complex were collected from 26 laboratories (in Australia, Canada, Europe, India, South and North America, and Taiwan) and published studies. Distributions that fulfilled CLSI criteria were pooled and ECVs were estimated. The sensitivity of three ECV analytical techniques (the ECOFFinder, normalized resistance interpretation [NRI], derivatization methods) to the inclusion of MICs for mutants was examined for three susceptibility testing methods (the CLSI, EUCAST, and Etest methods). The totals of posaconazole MICs for nonmutant isolates (isolates with no known cyp51A mutations) and mutant A. fumigatus isolates were as follows: by the CLSI method, 2,223 and 274, respectively; by the EUCAST method, 556 and 52, respectively; and by Etest, 1,365 and 29, respectively. MICs for 381 isolates with unknown mutational status were also evaluated with the Sensititre YeastOne system (SYO). We observed an overlap in posaconazole MICs among nonmutants and cyp51A mutants. At the commonly chosen percentage of the modeled wild-type population (97.5%), almost all ECVs remained the same when the MICs for nonmutant and mutant distributions were merged: ECOFFinder ECVs, 0.5 μg/ml for the CLSI method and 0.25 μg/ml for the EUCAST method and Etest; NRI ECVs, 0.5 μg/ml for all three methods. However, the ECOFFinder ECV for 95% of the nonmutant population by the CLSI method was 0.25 μg/ml. The tentative ECOFFinder ECV with SYO was 0.06 μg/ml (data from 3/8 laboratories). Derivatization ECVs with or without mutant inclusion were either 0.25 μg/ml (CLSI, EUCAST, Etest) or 0.06 μg/ml (SYO). It appears that ECV analytical techniques may not be vulnerable to overlap between presumptive wild-type isolates and cyp51A mutants when up to 11.6% of the estimated wild-type population includes mutants