Sistema de Gestión del Conocimiento ANLIS MALBRÁN
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Effective population size differences in Calomys musculinus, the host of Junín Virus: their relationship with the epidemiological history of Argentine Hemorrhagic fever
No full textFil: Chiappero, Marina B. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Diversidad y Ecología Animal; Argentina.Fil: Piacenza, María Florencia. Universidad Nacional de Río Cuarto. Departamento de Ciencias Naturales; Argentina.Fil: Provensal, María Cecilia. Universidad Nacional de Río Cuarto. Departamento de Ciencias Naturales; Argentina.Fil: Calderón, Gladys E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.Fil: Gardenal, Cristina N. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Diversidad y Ecología Animal; Argentina.Fil: Polop, Jaime J. Universidad Nacional de Río Cuarto. Departamento de Ciencias Naturales; Argentina.Argentine hemorrhagic fever (AHF) is a serious endemic disease in Argentina, produced by Junín virus, whose host is the Sigmodontinae rodent Calomys musculinus. Within the endemic area, human incidence and proportion of infected rodents remains high for 5-10 years after the first appearance of the disease (epidemic [E] zone) and then gradually declines to sporadic cases (historic [H] zone). We tested the hypothesis that host populations within the E zone are large and well connected by gene flow, facilitating the transmission and maintenance of the virus, whereas those in the H and nonendemic (NE) zones are small and isolated, with the opposite effect. We estimated parameters affected by levels of gene flow and population size in 14 populations of C. musculinus: population effective size (Ne), genetic variability, and mean relatedness. Our hypothesis was not supported: the lowest levels of variability and of Ne and the highest genetic relatedness among individuals were found in the H zone. Populations from the NE zone displayed opposite results, whereas those in the E zone showed intermediate values. If we consider that populations are first NE, then E, and finally H, a correlative decrease in Ne was observed. Chronically infected females have a low reproductive success. We propose that this would lower Ne because each cohort would originate from a fraction of females of the previous generation, and affect other factors such as proportion of individuals that develop acute infection, probability of viral transmission, and evolution of virulence, which would explain, at least partly, the changing incidence of AHF
Natural Snake Venom Inhibitors and their Pharmaceutical Uses: Challenges and Possibilities
No full textFil: Saavedra, Soledad Lorena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Ávila, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.Fil: Giudicessi, Silvana Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Albericio, Fernando. University of Barcelona. Department of Organic Chemistry; España.Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Fil: Martinez-Ceron, Maria Camila. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología; Argentina.Nowadays, treatment with specific antivenins is considered the only cure for snakebites accidents. However, access to antivenom obstructs the successful implementation of the World Health Organization international guidelines. In the last few years, natural organic compounds, peptides, and proteins with the ability to inhibit snake toxins and obtained from different sources such as plant extracts and animal blood have been proposed as antivenoms. In this work, we will focus on the inhibitors of the main venom toxins, phospholipases A2 and metalloproteinases, and their application as novel antivenoms
Successful management with fosfomycin + ceftazidime of an infection caused by multiple highly-related subtypes of multidrug-resistant and extensively drug-resistant KPC-producing Serratia marcescens
Get PDFFil: Rodriguez, Cecilia. Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas (CERELA-CONICET), San Miguel de Tucumán, Tucumán; Argentina.Fil: Brengi, Silvina P. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Enterobacterias; Argentina.Fil: Cáceres, Mariel Agustina. Laboratorio de Bacteriología del Hospital 'Ángel C. Padilla', San Miguel de Tucumán, Tucumán; Argentina.Fil: Mochi, Silvana. Laboratorio de Bacteriología del Hospital 'Ángel C. Padilla', San Miguel de Tucumán, Tucumán; Argentina.Fil: Viñas, María R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Enterobacterias; Argentina.Fil: Rizza, Constanza Antich. Laboratorio de Microbiología del Hospital del Niño Jesús, San Miguel de Tucumán, Tucumán; Argentina.Fil: Merletti, G. Laboratorio de Microbiología del Hospital del Niño Jesús, San Miguel de Tucumán, Tucumán; Argentina.Fil: Bru, Elena. Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas (CERELA-CONICET), San Miguel de Tucumán, Tucumán; Argentina.Fil: Assa, José Daniel. Laboratorio de Microbiología del Hospital del Niño Jesús, San Miguel de Tucumán, Tucumán; Argentina.Fil: Raya, Raúl Ricardo. Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas (CERELA-CONICET), San Miguel de Tucumán, Tucumán; Argentina.Fil: Centrón, Daniela. Instituto de Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires (IMPaM, UBA-CONICET), Buenos Aires, Argentina
Trichinella spiralis in a South American sea lion (Otaria flavescens) from Patagonia, Argentina
No full textFil: Pasqualetti, Mariana. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias, CABA; Argentina.Fil: Fariña, Fernando. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias, CABA; Argentina.Fil: Krivokapich, Silvio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Gatti, Graciana Mabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Daneri, G A. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de Ciencias Naturales, "Bernardino Rivadavia"- CONICET, CABA; Argentina.Fil: Varela, E. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de Ciencias Naturales, "Bernardino Rivadavia"- CONICET, CABA; Argentina.Fil: Lucero, S. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de Ciencias Naturales, "Bernardino Rivadavia"- CONICET, CABA; Argentina.Fil: Ercole, Mariano. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias, CABA; Argentina.Fil: Bessi, C. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias, CABA; Argentina.Fil: Winter, M. Universidad Nacional de Río Negro-Sede Atlántica, Viedma, Río Negro; Argentina.Fil: Ribicich, Mabel. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias, CABA; Argentina.Trichinella spp. from a sylvatic cycle has been found in several animal species such as pumas (Puma concolor), armadillos (Chaetophractus villosus), rats (Rattus norvegicus), and wild boars (Sus scrofa) in Argentina. Moreover, Trichinella infection has been detected in a wide range of marine mammals around the world, including polar bears (Ursus maritimus) and walruses (Odobenus rosmarus). Until the present time, Trichinella spp. infection has not been detected in marine mammals of South America. Samples from four South American sea lions (Otaria flavescens) found dead in Rio Negro, Argentina, were analyzed by artificial digestion, and in the case of one animal, Trichinella larvae were identified at the species level by nested multiplex PCR as Trichinella spiralis. This is the first report of a Trichinella species infecting marine mammals from South America
Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
No full textFil: Imaz, María S. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.Fil: Allassia, Sonia. Dirección de Bioquímica de la Municipalidad de Rosario. Rosario; Argentina.Fil: Aranibar, Mónica. Hospital San Roque. Jujuy; Argentina.Fil: Gunia, Alba M. Laboratorio Central de Salud Pública. Chaco; Argentina.Fil: Poggi, Susana. Hospital F.J. Muñiz. CABA; Argentina.Fil: Togneri, Ana M. Hospital Interzonal General de Agudos «Evita». Lanús, Buenos Aires; Argentina.Fil: Wolff, Lidia. Hospital Rawson. Córdoba; Argentina.To assess a LED-fluorescence microscopy (LED-FM) capacitation program for the training of laboratory technicians without previous experience in FM
Quality assurance for antimicrobial susceptibility testing of Neisseria gonorrhoeae in Latin American and Caribbean countries, 2013-2015
No full textFil: Sawatzky, Pam. Streptococcus and STI Unit, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba; Canada.Fil: Martin, Irene. Streptococcus and STI Unit, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba; Canada.Fil: Galarza, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Carvallo, Marıa Elena Trigoso. Programa de ITS/VIH/SIDA, Laboratorio del Centro Departamental de Vigilancia, Informacion y Referencia, La Paz; Bolivia.Fil: Araya Rodriguez, Pamela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Cruz, Olga Marina Sanabria. Grupo de Microbiología, Instituto National de Salud, Bogota; Colombia.Fil: Hernandez, Alina Llop. Laboratorio Nacional de Referencia de Microbiología, Instituto de Medicina Tropical 'Pedro Kouri' (IPK), La Habana; Cuba.Fil: Martinez, Mario Fabian. Seccion Antimicrobianos, Laboratorio Central De Salud Pública, Asunción; Paraguay.Fil: Borthagaray, Graciela. Laboratorio de Microbiologia, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo; Uruguay.Fil: Payares, Daisy. Departamento de Bacteriologia, Instituto Nacional de Higine 'Rafael Rangel', Caracas; Venezuela.Fil: Moreno, José E. Laboratorio Central de Referencia en Salud Publica, Instituto Conmemeorahvo Gorgas de Estudios de la Salud, Panamá; Panamá.Fil: Chiappe, Marina. Facultad de Salud Pública y Administración, Universidad Peruana Cayetano Heredia, Lima; Peru.Fil: Corredor, Aura Helena. Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan; Canada.Fil: Thakur, Sidharath Dev. Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan; Canada.Fil: Dillon, Jo-Anne R. Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan; Canada.A Neisseria gonorrhoeae antimicrobial susceptibility quality control comparison programme was re-established in Latin America and the Caribbean to ensure antimicrobial susceptibility data produced from the region are comparable nationally and internationally
PCV13 vaccination impact: A multicenter study of pneumonia in 10 pediatric hospitals in Argentina
Get PDFFil: Gentile, Angela. Hospital de Niños Dr. Ricardo Gutiérrez. Epidemiología, Buenos Aires; Argentina.Fil: Bakir, Julia. Hospital de Niños Dr. Ricardo Gutiérrez. Epidemiología, Buenos Aires; Argentina.Fil: Firpo, Verónica. Hospital de Niños Dr. Ricardo Gutiérrez. Epidemiología, Buenos Aires; Argentina.Fil: Casanueva, Enrique V. Hospital del Niño de San Justo. Infectología, Buenos Aires; Argentina.Fil: Ensinck, Gabriela. Hospital de Niños Víctor J. Vilela. Infectología, Rosario, Santa Fe; Argentina.Fil: Lopez Papucci, Santiago. Hospital de Niños Víctor J. Vilela. Infectología, Rosario, Santa Fe; Argentina.Fil: Lución, María F. Hospital de Niños Dr. Ricardo Gutiérrez. Epidemiología, Buenos Aires; Argentina.Fil: Abate, Hector. Hospital de niños Dr.Humberto Notti. Infectología, Mendoza, Argentina.Fil: Cancellara, Aldo. Hospital General de Niños Pedro de Elizalde. Infectología, Buenos Aires; Argentina.Fil: Molina, Fabiana. Hospital de Niños Dr. Orlando Alassia. Clínica, Santa Fe; Argentina.Fil: Gajo Gane, Andrea. Hospital Pediátrico Juan Pablo II. Infectología, Corrientes; Argentina.Fil: Caruso, Alfredo M. Hospital Materno Infantil Dr. Héctor Quintana. Infectología, Jujuy; Argentina.Fil: Santillán Iturres, Alejandro. Hospital de Niños Eva Perón. Infectología, Catamarca; Argentina.Fil: Fossati, Sofía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.In 2012, PCV13 was introduced into the National Immunization Program in Argentina, 2+1 schedule for children <2 years. Coverage rates for 1st and 3rd doses were 69% and 41.0% in 2012, 98% and 86% in 2013; 99% and 89% in 2014, respectively. The aims of this study were to evaluate impact of PCV13 on Consolidated Pneumonia (CP) and Pneumococcal Pneumonia (PP) burden, and to describe epidemiological-clinical pattern of PP during the three-year period following vaccine introduction
Virulence profile of Escherichia coli O157 strains isolated from surface water in cattle breeding areas
No full textFil: Tanaro, J D. Cátedra de Microbiología, Facultad De Bromatología, Universidad Nacional de Entre Ríos, Gualeguaychú; Argentina.Fil: Pianciola, Luis. Laboratorio Central, Subsecretaría de Salud de Neuquén, Neuquén; Argentina.Fil: D'Astek, Beatriz A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Piaggio, Mercedes C. Cátedra de Microbiología, Facultad De Bromatología, Universidad Nacional de Entre Ríos, Gualeguaychú; Argentina.Fil: Mazzeo, Melina. Laboratorio Central, Subsecretaría de Salud de Neuquén, Neuquén; Argentina.Fil: Zolezzi, Gisela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spill around drinking troughs, and small water courses and lagoons, formed by runoff). Nineteen (50·0%) strains harboured stx2a /stx2c genes, 18 (47·4%) stx2c and one stx1a /stx2c . All strains harboured eae, ehxA, rfbO157 and fliCH7 genes, and the putative virulence determinants ECSP_0242, ECSP_2687 and ECSP_3620. All isolates tested as Lineage I/II by lineage-specific polymorphism assay-6. Nineteen (50%) belonged to the high virulent clade 8. The q21 allele was found in all strains and q933 /q21 alleles in 17 (44·7%). By XbaI-pulsed-field gel electrophoresis, 29 strains were grouped into seven clusters. Four clusters grouped isolates from distant places separated by 150-250 km. This may be related to vectors, like birds, involved in their spread. Otherwise, three clusters contained isolates recovered at same places with intervals of 1-9 months. This could be explained by the high environmental persistence of STEC O157:H7. These strains recovered from surface water showed similar genotypes to those found in the bovine reservoir and in human diseases, and could be linked to the high incidence of haemolytic uremic syndrome in Argentina
Bacteriemia por Mycoplasma hominis: un agente etiológico subestimado
No full textFil: Posse, Tamara. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Fil: Prieto, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Especial; Argentina.Fil: Cipolla, Lucia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Especial; Argentina.Fil: Kaufman, Sara. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Bacteriología Clínica; Argentina.Resumen
Mycoplasma hominis es una bacteria de cultivo exigente y forma parte de la microbiota comensal de la zona urogenital en adultos. Puede ocasionar infecciones del tracto genitourinario, en particular en mujeres, e infecciones sistémicas en neonatos. Además, puede causar infecciones extragenitales graves, en especial en pacientes inmunocomprometidos. Describimos un caso de bacteriemia por M. hominis en una paciente inmunocompetente, luego de un legrado uterino por aborto incompleto. M. hominis está subestimado como agente etiológico de infecciones extragenitales debido a su difícil diagnóstico, ya que al carecer de pared celular no se visualiza por la coloración de Gram, requiere una incubación prolongada en atmósfera de anaerobiosis para su desarrollo y los métodos convencionales de detección pueden fallar. Este es el primer reporte de señal positiva en hemocultivo automatizado (BD BACTEC) con aislamiento de M. hominis.
Abstract
Mycoplasma hominis is a fastidious bacterium, which usually colonizes the lower urogenital tract and may cause systemic infections in neonates and genital infections in adults. It can also be the cause of serious extra-genital infections, mainly in immunosuppressed or predisposed subjects. Case Presentation: We describe a case of bacteremia caused by M. hominis in a previously healthy woman after uterine curettage due to incomplete abortion. M. hominis could be an underestimated cause of bacteremia in immunocompetent patients. Mycoplasma organisms have fastidious growth requirements, are often difficult to culture on a cell-free medium and have no cell wall. The conventional method for detection may fail. This is the first report of M. hominis isolation from a positive automated blood culture (BD BACTEC, USA)
Co-occurrence and seasonal and environmental distributions of the sandflies Lutzomyia longipalpis and Nyssomyia whitmani in the city of Puerto Iguazú, northeastern Argentina
Get PDFFil: Santini, María Soledad. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación en Endemo Epidemias; Argentina.Fil: Fernandez, Maria S. Red de Investigación de la Leishmaniasis en Argentina; Argentina.Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.Fil: Salomón, Oscar Daniel. Ministerio de Salud de la Nación. Instituto Nacional de Medicina Tropical; Argentina.The aim of this work was to study the distribution of Phlebotominae (Diptera: Psycodidade) abundance in time and space in an area in northeastern Argentina with vector transmission of visceral and tegumentary leishmaniasis. For this, 51 households were selected using a 'worst scenario' criterion where one light trap was set during two consecutive nights in peridomiciles in the transitions between the four seasons, and the environment was surveyed simultaneously. The relationships of phlebotomine assemblage structure and the most abundant species with seasonality and environmental variables were evaluated using a canonical correspondence analysis and generalized linear mixed models, respectively. A total of 5110 individuals were captured. Lutzomyia longipalpis (Lutz & Neiva, 1912) and Nyssomyia whitmani (Antunes & Coutinho, 1939) were the most abundant species captured in all samplings (98.3% of the total capture). The period of highest abundance of Lu. longipalpis was early autumn, and it was distributed in the most urbanized areas. Nyssomyia whitmani occupied mainly the less urbanized areas, showing peaks of abundance in early spring and summer. Other species were captured in low numbers and showed seasonal-spatial variations similar to those of Ny. whitmani. We confirmed Leishmania spp. vector persistence throughout the year in spatial patches of high abundance even during the less favorable season