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The regression of endometriosis with glycosylated flavonoids isolated from Melilotus officinalis (L.) Pall. in an endometriosis rat model
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Beliefs and preferences regarding biological treatments for severe asthma
No full textBackground: Severe asthma is a serious condition with a significant
burden on patients' morbidity, mortality, and quality of life. Some
biological therapies targeting the IgE and interleukin-5 (IL5) mediated
pathways are now available. Due to the lack of direct comparison
studies, the choice of which medication to use varies. We aimed to
explore the beliefs and practices in the use of biological therapies in
severe asthma, hypothesizing that differences will occur depending on
the prescribers' specialty and experience.
Methods: We conducted an online survey composed of 35 questions in
English. The survey was circulated via the INterasma Scientific Network
(INESNET) platform as well as through social media. Responses from
allergists and pulmonologists, both those with experience of prescribing
omalizumab with (OMA/IL5) and without (OMA) experience with anti-IL5
drugs, were compared.
Results: Two hundred eighty-five (285) valid questionnaires from 37
countries were analyzed. Seventy-on percent (71\%) of respondents
prescribed biologics instead of oral glucocorticoids and believed that
their side effects are inferior to those of Prednisone 5 mg daily.
Agreement with ATS/ERS guidelines for identifying severe asthma patients
was less than 50\%. Specifically, significant differences were found
comparing responses between allergists and pulmonologists (Chi-square
test, p < 0.05) and between OMA/IL5 and OMA groups (p < 0.05).
Conclusions: Uncertainties and inconsistencies regarding the use of
biological medications have been shown. The accuracy of prescribers to
correctly identify asthma severity, according to guidelines criteria, is
quite poor. Although a substantial majority of prescribers believe that
biological drugs are safer than low dose long-term treatment with oral
steroids, and that they must be used instead of oral steroids, every
effort should be made to further increase awareness. Efficacy as disease
modifiers, biomarkers for selecting responsive patients, timing for
outcomes evaluation, and checks need to be addressed by further
research. Practices and beliefs regarding the use of asthma biologics
differ between the prescriber's specialty and experience; however, the
latter seems more significant in determining beliefs and behavior.
Tailored educational measures are needed to ensure research results are
better integrated in daily practice
Iron homeostasis is altered in response to hypoxia and hypothermic preconditioning in brain glial cells
No full textBackground/aim: Altered iron metabolism is one of the pathophysiological
mechanisms occurring during hypoxic injuries in the central nervous
system. Proper homeostasis of cellular iron is regulated by iron import,
storage, and export proteins that prevent excess iron overload or iron
starvation in cells. Therapeutic hypothermia is an approved treatment
for hypoxic ischemia in newborns, but the underlying molecular mechanism
is still unknown. We studied the effects of hypoxia, preceded with
preconditioning, on the iron homeostasis of glial cells, known as a
major actor in the inflammatory process during perinatal brain injury.
Materials and methods: Primary microglia and astrocytes in culture were
exposed to 12 h of hypoxia with or without mild hypothermic
preconditioning. The mRNA expression was assessed using qPCR. Iron
accumulation was visualized via modified Perl's histochemistry. Cytokine
levels in cell cultures were measured using ELISA.
Results: Hypothermic preconditioning enhanced microglial viability,
which previously was decreased in both cell types due to hypoxia.
Hypoxia increased iron accumulation in the mixed glial cells and in
ferritin expression in both microglia and astrocytes. Hypotermic
preconditioning decreased the elevated ferritin-light chain expression
significantly in microglia. Iron importer proteins, DMT1 and TfR1, both
increased their mRNA expression after hypoxia, and hypothermic
preconditioning continued to support the elevation of DMT1 in both glial
cell types. Ferroportin expression increased as a survival factor of the
filial cell following hypoxia. Hypothermic preconditioning supported
this increase in both cell types and was especially significant in
astrocytes. IL-10 levels were prominently increased in cell culture
after hypothermic preconditioning.
Conclusion: The data suggest that hypothermic preconditioning affects
cellular iron homeostasis by regulating the storage and transfer
proteins of iron. Regulation of the cellular iron traffic may prevent
glial cells from experiencing the detrimental effects of hypoxia-related
inflammation
Acute Complications and Pregnancy Outcomes with Continuous Subcutaneous Insulin Infusion Therapy in Pregnant Women with Type 1 Diabetes Mellitus
No full textÖğretmenlerin Bilim Merkezlerinde Öğrenmeye Yönelik Mesleki Gelişim İhtiyaçlarının Araştırılması
No full textValidity of six month l-thyroxine dose for differentiation of transient or permanent congenital hypothyroidism
No full textThe evaluation of the oxidative stress index of reproductive tissues and serum thiol/disulphide homeostasis during estrous cycle in bitches [Köpeklerde östrus siklusu boyunca reprodüktif dokularda oksidatif stres indeksi ve serum tiyol/disülfit homestazının değerlendirilmesi]
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