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The evaluation of the oxidative stress index of reproductive tissues and serum thiol/disulphide homeostasis during estrous cycle in bitches
The aim of this study was firstly to determine the relationship between
estrous cycle and oxidative stress in reproductive tissues in bitches.
This research was performed in twenty-nine healthy bitches from
different breeds and of varied ages (the range was 2-5 years) that were
brought to the clinic for routine ovariohysterectomy. The stages of
estrous cycle were detected using by vaginal cytology, blood
progesterone level and histological findings. Ovarian, oviduct and
uterine tissues were taken into Eppendorf tubes for oxidative stress
index and stored at -80 degrees C until analyses. Also, another part of
these tissues were fixed in 10\% formalin solution. There were no
significant differences (P > 0.05) among total antioxidant status (TAS),
total oxidant status (TOS) and oxidative stress index (OSI) values in
the reproductive tissues in concerning estrous cycle stages. However,
there were remarkable correlations between oxidative stress parameters
and reproductive tissues in different estrus stages in bitches. In
conclusion, the physiological values of TAS and TOS concentration, and
OSI in the ovarian, oviduct and uterine tissues during estrous cycle
were firstly defined in this article. The serum thiol/disulphide
homeostasis was also determined in the estrous cycle of bitches. We have
found that there are remarkable variations of oxidative stress balance
in the ovary, oviduct and uterus concurrently, during estrous cycle in
the bitches. Besides, significant correlations between oxidative stress
parameters and estrous cycle stages in the reproductive tissues were
observed in the present study