Gazi University Dspace
Not a member yet
26981 research outputs found
Sort by
Application of vascular endothelial growth factor at different phases of intestinal ischemia/reperfusion: What are its effects on oxidative stress, inflammation and telomerase activity?
No full textBackground. Intestinal ischemic reperfusion injury (IRI) represents a
great challenge in clinical practice, with high morbidity and mortality.
Vascular endothelial growth factor (VEGF), as a signal protein,
contributes to vasculogenesis and angiogenesis.
Objectives. To evaluate the local effectiveness of VEGF following
intestinal IRI and its relation with application time.
Material and methods. Thirty Wistar albino rats were allocated to 5
groups and underwent laparotomy. The superior mesenteric arteries (SMA)
were dissected in 4 groups, while the control group (Gr C) underwent a
resection of small and large intestines. The VEGF group (Gr V) received
VEGF following SMA dissection, with no further intervention, and the
remaining 3 groups were subjected to ischemia for 90 min through
occlusion of SMA and reperfusion for 4 h. Ischemic reperfusion group (Gr
I/R) received no additional medication, while the remaining 2 groups
received VEGF just before ischemia (Gr V+I/R) and during reperfusion (Gr
I/R+V).
Results. Both applications of VEGF caused decreases in plasma levels of
interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha),
intestinal malondialdehyde (MDA), oxidized glutathione, protein carbonyl
levels, and increases in intestinal total glutathione and superoxide
dismutase (SOD) levels. Telomerase activity, which disappeared for Gr
I/R, was found to be elevated following both treatment groups.
Similarly, the histopathological scores were found better for both
treatment groups, but Gr V-I/R represented best outcomes.
Conclusions. The findings of our study revealed that VEGF, applied
either before ischemia or during reperfusion, is effective on local
damage following intestinal IRI. By interpreting the biochemical
analysis and histopathological findings, we conclude either treatment
option to be considered according to the reason of intestinal IRI
Mobil Telefon Kullanıcılarının Mobil Bağımlılık Durumu ve 5G Teknolojisi Kabullenme Niyeti Modellerinin Geliştirilmesi
No full textA medium frequency transformer design for spot welding machine using sizing equation and finite element analysis
No full textKnowledge and View of Mothers Whose Babies in Newborn Intensive Care Units About Breast Milk Banking in Turkey
No full textReal-life comparison of the viewing angle and the image quality of two commonly used viewing systems for vitreoretinal surgery
No full textComparıson Of In Vıtro Genotoxıc Effects Of Needle-Lıke And Spherıc Tıtanıum Dıoxıde (Tio2) Nanopartıcles (Nps) In Human Perıferal Blood Lymphocyte
No full textBu çalışmada, 100 nm’nin altındaki iğnemsi (İ) Titanyum Dioksit Nanopartikülleri (TDNP-İ) ile
küresel (K) Titanyum Dioksit Nanopartiküllerinin (TDNP-K) insan periferal lenfositlerindeki
genotoksik etkileri kromozom anormallikleri (KA), kardeş kromatit değişimi (KKD) ve
mikronukleus (MN) testleri ile incelenmiştir. Lenfositler, her iki nanopartikülün 125, 250, 500 ve
1.000 μg/mL’lik konsantrasyonlarına, 24 ve 48 saat maruz bırakılmıştır. 24 saatlik uygulamada,
TDNP-İ’nin tüm konsantrasyonları KA frekansı ve KA/Hücre sayısını negatif kontrole kıyasla
artırmıştır. Ancak bu artışlar, sadece 250 ve 1.000 μg/mL’lik konsantrasyonlarda anlamlı
düzeydedir. 48 saatlik uygulamada, hem anormal hücre frekansında ve hem de KA/Hücre sayısında
artış ve hafif azalışlar olmakla birlikte, sadece 1.000 μg/mL’lik konsantrasyondaki artışlar hem
negatif ve hem de çözücü kontrole kıyasla anlamlıdır. TDNP-K’nın da 24 saatlik uygulamasında,
hem KA frekansında ve hem de KA/Hücre sayısında artışlar tespit edilmiştir. Ancak bu artış, KA
frekansında sadece 1.000 μg/mL’de (çözücü kontrole kıyasla), KA/Hücre sayısında ise sadece 500
μg/mL’de hem negatif ve hem de çözücü kontrole kıyasla anlamlıdır. 48 saatlik uygulamada ise,
hem KA frekansında ve hem de KA/Hücre sayısında artış ve hafif azalışlar olmakla birlikte, 250,
500 ve 1.000 μg/mL’de, KA/Hücre sayısının çözücü kontrole kıyasla anlamlı düzeyde yüksek
olduğu tespit edilmiştir. TDNP-İ’nin 24 saatlik uygulamasında, 250 ve 500 μg/mL’de, KKD
sayısında çözücü kontrole kıyasla anlamlı düşüş gözlenirken, 125 ve 1.000 μg/mL’de anlamlı artış
tespit edilmiştir. 48 saatlik uygulamada, KKD sayısı, çözücü kontrole kıyasla, 125 μg/mL’de
anlamlı azalış gösterirken, diğer tüm konsantrasyonlarda anlamlı artış göstermiştir. TDNP-K’nın
hem 24 ve hem de 48 saatlik uygulamasında, 500 ve 1.000 μg/mL’lik konsantrasyonlarda KKD
sayısında çözücü kontrole kıyasla anlamlı artış tespit edilmiştir. MN testinde, TDNP-İ 500 ve 1.000
μg/mL’de, TDNP-K ise sadece 1.000 μg/mL’de, MN frekansını çözücü kontrole kıyasla anlamlı
düzeyde artırmıştır. Mitotik İndeks (MI)’in, TDNP-İ’in 24 saatlik uygulamasında 1.000 μg/mL’de
çözücü kontrole kıyasla anlamlı düşüş gösterdiği, diğer konsantrasyonlarda ise artış gösterdiği, bu
artışların sadece 250 μg/mL’de negatif kontrole kıyasla anlamlı olduğu belirlenmiştir. 48 saatlik
uygulamada, MI’in 500 ve 1.000 μg/mL’de hem negatif ve hem de çözücü kontrole kıyasla anlamlı
düşüş gösterdiği belirlenmiştir. TDNP-K uygulamasında, 24 saatte 1.000 μg/mL’de (hem negatif ve
hem de çözücü kontrole kıyasla), 48 saatte 250, 500 ve 1.000 μg/mL’de negatif kontrole kıyasla
anlamlı düzeyde düştüğü belirlenmiştir. Bu iki nanopartikül Nükleer Bölünme İndeksi (NBI) ve
Replikasyon İndeksinde (RI), kontrollere kıyasla anlamlı bir fark oluşturmamıştır. Bu sonuçlar, her
iki formdaki nanopartikülün zayıf sitotoksik, genotoksik ve klastojenik olduğunu göstermektedirIn this study, genotoxic effects of needle-like Titanium Dioxide Nanoparticles (TiO2 NP-N) and
spheric Titanum Dioxide Nanoparticles (TiO2 NP-S) below 100 nm were examined in human
peripheral lymphocyte by using Chromosome Aberration (CA), Sister Chromatid Exchange (SCE)
and Micronucleus (MN) assays. Lymphocytes were exposed to 125, 250, 500 and 1,000 μg/mL
concentrations of both nanoparticles for 24 h and/or 48h. At 24h treatment, all the concentrations of
TiO2 NP-N increased the frequency of aberrant cells and CA/cell number compared to negative
control. However, this increase was significant at the 250 and 1,000 μg/mL concentrations.
Although there was an increase and a slight decrease in the frequency of aberrations and in CA/Cell
at 48 h treatment, the values observed following 1,000 μg/mL showed statistically significant
increase compared to negative and solvent controls. TiO2 NP-S, at 24 h treatment, increased the
frequency of CAs and CA/Cell compared to negative control. However increase in the frequency of
CAs was statistically significant at 1,000 μg/mL (compared to solvent control) and, was statistically
significant at 500 μg/mL in CAs/Cell compared to both negative and solvent controls. At 48 h
treatment, while both increasing and decreasing were observed in the frequency of aberrations and
CAs/Cell, only CA/Cell statistically increased at 250, 500 and 1,000 μg/mL compared to solvent
control. While TiO2 NP-N, at 24 h exposure, significantly reduced SCE/Cell at 250 and 500 μg/mL
compared to solvent control, it significantly increased SCE/Cell at 125 and 1,000 μg/mL. At 48 h
exposure, while SCE/Cell significantly reduced at 125 μg/mL compared to negative control, all the
other concentrations (250, 500, and 1,000 μg/mL) significantly increased the same parameter. TiO2
NP-S significantly increased the number of SCEs at 500 and 1,000 μg/mL compared to solvent
control. TiO2 NP-N at 500 ve 1,000 μg/mL significantly increased the frequency of MN compared
to solvent control. In Mitotic Index, at 24 h exposure time, while significant reduction was
observed at 1,000 μg/mL following TiO2 NP-N exposure compared to solvent control, increasing
was evident at all the other concentrations, among them only 250 μg/mLwas significant compared
to negative control. At 48 h exposure, MI significantly reduced at 500 and 1,000 μg/mL compared
to both negative and solvent controls. TiO2 NP-S treatment decreased MI at 1,000 μg/mL for 24 h
(compared to both negative and solvent controls) and, at 250, 500, and 1,000 μg/mL for 48 h
compared to negative control. Neither of these nanoparticles affects nuclear division index and
replication index compared to controls. These results show that both forms of nanoparticles induce
low cytotoxic, genotoxic and clastogenic effect
Determination of Myocardial Scar Tissue in Coronary Slow Flow Phenomenon and The Relationship Between Amount of Scar Tissue and Nt-ProBNP
No full textBackground: Pathophysiology and prognosis are not clearly determined in
patients with the coronary slow flow phenomenon (CSFP). These patients
present with various clinical conditions ranging from being asymptomatic
to being admitted with sudden cardiac death.
Objectives: We aimed at assessing the findings of late gadolinium
enhancement (LGE) in cardiac magnetic resonance imaging (CMR) as an
indicator of myocardial fibrosis. We also aimed at determining the
relationship between the presence of myocardial fibrosis and NT-proBNP
levels in patients with CSFP in the left anterior descending coronary
artery (LAD).
Methods: A total of 35 patients were enrolled within an age range of
31-75. The study patients (n=19) had normal epicardial coronary arteries
at angiography, but they presented with CSFP in the LAD. The control
group patients (n=16) had normal epicardial coronary arteries and TIMI
scores at normal levels in angiography. In both groups, the patients
were examined with CMR for the presence of myocardial fibrosis. In
addition, plasma NT-proBNP levels were measured. A p-value < 0.05 was
considered significant.
Results: The rate of myocardial fibrosis was significantly higher in CMR
in the patients with CSFP (p=0.018). A variable amount of myocardial
scar tissue was detected at the left ventricular apex in 7 patients and
at the inferior and inferolateral regions in 3 patients. There was no
difference in the level of NT-proBNP in patients with CSFP. However, the
NT-proBNP levels were higher in patients with CSFP, who had scar tissue
in CMR (p=0.022).
Conclusions: In conclusion, LGE in CMR showed that ischemic myocardial
scarring may exist in patients with CSFP. These results indicate that
CSFP may not always be innocent
