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    The comparison of the applicability of low-frequency laser, β-tricalcium phosphate, platelet-rich fibrin, and bone marrow in the treatment of tibial fractures in rabbits

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    PURPOSE: To compare and evaluate the radiologic, clinical, and histopathologic results of the treatment methods applied in diaphyseal tibial fractures. METHODS: A complete tibial fracture was created in the tibial diaphysis in each rabbit. Experimentally generated fracture fragments were fixed by intramedullary pinning. In the control group (group I), the bone fracture area was left to heal without any treatment technique. Group II received low-energy laser therapy once daily to the surgical side for 30 days; group III, autologous bone marrow aspirated from the left proximal tibia; group IV, a combination of bone marrow obtained by aspiration and synthetic β-tricalcium phosphate (β-TCP); and in group V, a platelet-rich fibrin (PRF) membrane obtained from the central auricular artery was applied to the fracture side. RESULTS: In the X-ray analysis, it was determined that group IV had the fastest score increase, while group I had the lowest scores. In group IV, no lameness that persisted until the end of the study was observed in any rabbit. When the histopathological scores of the different groups were examined, it was seen that the lowest score belonged to group I, and the highest score was in group IV. CONCLUSION: The highest rate of new bone formation and bone regeneration was achieved when the combination of aspirated bone marrow and β-TCP granules was applied. The experimental group with PRF membrane application exhibited the least osteogenic characteristics among all experimental groups

    16S RRNA-BASED IDENTIFICATION OF ENDOLICHENIC BACTERIA FROM RHIZOPLACA MELANOPHTHALMA (DC.) LEUCKERT & POELT LICHEN COLLECTED FROM MOUNT ERCIYES

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    Bu çalışmada, Türkiye’nin yüksek rakımlı ekosistemlerinden biri olan Erciyes Dağı’ndan toplananRhizoplaca melanophthalma (DC.) Leuckert &amp; Poelt liken türü içerisindeki endolikenik bakterilerinmoleküler tanımlanması ve filogenetik ilişkilerinin ortaya konulması amaçlanmıştır. Likenlerinekstrem çevre koşullarına karşı yüksek dayanıklılıkları, bünyelerinde barındırdıkları endolikenikmikroorganizmaların biyoteknolojik açıdan değerli olabileceğini düşündürmektedir. Bu kapsamdayüzey sterilizasyonu uygulanan liken örneklerinden toplam 3 bakteri izolatı elde edilmiştir. İzolatların16S rRNA gen bölgeleri dizilenmiş, elde edilen sekanslar BioEdit programı kullanılarak hizalanmış vefilogenetik analizler MEGA 7 yazılımı ile gerçekleştirilmiştir. Analizler sonucunda izolatların Bacilluscereus, Bacillus subtilis ve Enterococcus faecalis türlerine ait olduğu belirlenmiştir.Bu çalışma, Rhizoplaca melanophthalma kaynaklı endolikenik bakterilerden elde edilen ilk molekülerverilerden birini sunması bakımından önem taşımaktadır. Elde edilen sonuçlar, liken kökenlimikroorganizmaların tür düzeyinde çeşitlilik gösterebildiğini ortaya koymakta ve bumikroorganizmaların ilerleyen çalışmalarda biyolojik aktivite testleriyle değerlendirilerek potansiyelbiyoteknolojik uygulamalara kazandırılabileceğini düşündürmektedir.</p

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