Repositorio Institucional INIA (Inst. Nacional de Innovacion Agraria)
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Resolución Ministerial N° 0243-2024-MIDAGRI
Get PDF7 páginasReconocer la Zona de Agrobiodiversidad denominada “Circa”, ubicada en el distrito de Circa, provincia de Abancay, departamento de Apurímac, con una extensión de 53,454.4540 has. Zona con alta diversidad de raíces y tuberosas andinas, maíz cereales, leguminosas, granos andinos y plantas medicinales
Complete chloroplast genome of a Peruvian landrace of Cucurbita moschata, loche, and its comparative analysis with other relative species
Get PDFBackground Loche (Cucurbita moschata) is a pre-Columbian squash that is cultivated exclusively in the northern coast of Peru, Lambayeque. This crop is notable for the presence of warts in its skin and for its vegetative reproduction as it commonly lacks of seeds in fruits. Currently, loche may be considered a new product for international markets, recognizing the gastronomy of Lambayeque in the world and dynamizing the Peruvian agro-export area. However, genomic information about this squash is very limited.This research was funded by the project "Creación del servicio de agricultura de precisión en los Departamentos de Lambayeque, Huancavelica, Ucayali y San Martín 4 Departamentos" of the Ministry of Agrarian Development and Irrigation (MIDAGRI) of the Peruvian Government with grant number CUI 2449640
Reporte de Repositorio Institucional del 01 al 31 de Marzo 2024
No full textDurante el mes de marzo se incorporaron 20 publicaciones técnico científicas, en el Repositorio Institucional del INIA, contando a la fecha con un total de 2337 publicaciones, divididas en comunidades y colecciones. El objetivo de este reporte es mantener actualizados los datos sobre las publicaciones técnico-científicas que vienen siendo incorporadas por el área a cargo de la administración del Repositorio Institucional del INIA
Effect of pruning height and organic fertilization on the morphological and productive characteristics of Moringa oleifera Lam. in the Peruvian dry tropics
Get PDFThe objective of the study was to evaluate the effect of pruning height (PH) and organic fertilization dose (FD) on the morphology and productive characteristics of Moringa oleifera Lam. We germinated seeds collected from 10-year-old shrubs, and 2-month-old seedlings were transplanted in the final field. We used a two-factor design of PH (PH1:0.4, PH2:0.8, and PH3:1.2m from the ground) and FD (FD0:0, FD1:500, FD2:750, and FD3:1,000 g of decomposing goat manure). We carried out an initial pruning 4 months after transplanting and the harvests every 45 days. After three consecutive harvests, PH3 improved N° branches (12.53 ± 3.09) and dry matter (21.98 ± 1.30%), but PH1 showed greater stem lengths (1.65 ± 0.24 m) (p 0.05). The PH × FD interaction can improve the plant diameter (p < 0.01) and dry matter (p < 0.05) with PH2 (56.79 ± 3.71 mm) and PH3 (23.20 ± 1.04%) from FD1. We found an increasing trend in N° branches, plant diameter (p < 0.01), and the leaf–stem ratio. However, in the third harvest, the biomass production trend was downward for a short period for an adequate replacement of nutrients from the incorporated organic fertilizer. It is recommended to prune M. oleifera at 1.2m from the ground to stimulate greater biomass and maintain the leaf–stem ratio throughout the evaluated harvests and apply more than 500 g of goat manure after each harvest to restore the nutrients extracted from the soil.This research was funded by Project “Mejoramiento de la disponibilidad y acceso del material genético mediante el uso de técnicas de biotecnología reproductiva en ganado bovino tropical en las regiones de San Martín, Loreto y Ucayali - PROMEG Tropical” with CUI N. 2338934
Using UAV images and phenotypic traits to predict potato morphology and yield in Peru
No full textPrecision agriculture aims to improve crop management using advanced analytical tools.In this context, the objective of this study is to develop an innovative predictive model to estimate the yield and morphological quality, such as the circularity and length–width ratio of potato tubers, based on phenotypic characteristics of plants and data captured through spectral cameras equipped on UAVs. For this purpose, the experiment was carried out at the Santa Ana Experimental Station in the central Peruvian Andes, where advanced potato clones were planted in December 2023 under three levels of fertilization. Random Forest, XGBoost, and Support Vector Machine models were used to predict yield and quality parameters, such as circularity and the length–width ratio. The results showed that Random Forest and XGBoost achieved high accuracy in yield prediction (R2 > 0.74). In contrast, the prediction of morphological quality was less accurate, with Random Forest standing out as the most reliable model (R2 = 0.55 for circularity). Spectral data significantly improved the predictive capacity compared to agronomic data alone. We conclude that integrating spectral índices and multitemporal data into predictive models improved the accuracy in estimating yield and certain morphological traits, offering key opportunities to optimize agricultural management
Genetic diversity and structure of creole cattle (Bos taurus) from southern Peruvian Highlands
No full textThe creole cattle was originated after the introduction of cattle into America 5 centuries ago. Currently, the production traits of Peruvian creole cattle is scarcely known. An important characteristic of the creole cattle is its adaptability to different extreme environments. However, to date, molecular studies in Peruvian creole cattle are still scarce.
Currently, due to the advances in molecular genetics, a new generation of molecular markers has been developed for the genetic characterization of livestock. Single nucleotide polymorphisms (SNP) have become a very popular tool for the genetic study of livestock populations. Genome-wide SNP chips were developed for multi-breed genetic studies in cattle
Draft genome and SSR data mining of a Peruvian landrace of Capsicum chinense, the arnaucho chili pepper
No full textThe Arnaucho chili pepper (ACP) is a traditional vegetable used in Peru because of its gastronomic properties. Due to its importance in the Peruvian diet and economy, this species is a resource that can be a candidate to plant breeding programs. In this study, the complete genome nucleotide sequence of this chili pepper was generated using the Illumina Hiseq 2500 sequencing technology. We sequenced the whole genome of the ACP using a paired-end 150 strategy, obtaining 330.46 GB of sequencing data. The genome size of the ACP was 2.98 Gb with a contig N50 of 237 Mb and 95.39% complete BUSCOs. Also, we identified 71.96% of repetitive DNA of the genome assembly, of which retroelements occupy 37.95% of the total genome. We downloaded genomes of the Solanoideae subfamily and conducted a comparative analysis of simple sequence repeats (SSRs) with our draft genome, and we identified lower number of SSRs in the ACP genome compared to other pepper species. This first ACP genome is expected to contribute to a better understanding of its genetics to adapt to the arid conditions of the Peruvian coastal ecosystem and evolution
Sustainability of Kikuyu Grass (Pennisetum clandestinum) for the Development of Highland Livestock Farming in the Northern Macro-Region of Peru
No full textDisclaimer/Publisher’s Note: The statements, opinions, and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions, or products referred to in the content.The productive sustainability of Pennisetum clandestinum in the Peruvian highlands was evaluated through productivity, growth and chemical composition. The effect of the nitrogen (N2) dose applied to the soil, the use of organic matter and the frequency of cutting or phenological age on the productive yields, chemical composition, plant height and growth rate of Kikuyu were determined. Experimentation was carried out under a randomised block design in divided plots. A multivariate analysis of variance (MANOVA) was used to determine the associated differences in each study factor. It was determined that applying 120 kg of N2 increases the annual protein production to 3 454.53 kg ha-¹. yr-¹, and a crude protein (CP) level of 23.54%, the distance of the live fences influences the biomass production to 19 176.23 kg DM ha-¹ at an interval of 8.5 to 11.5 meters from the base of the cypress tree (Cupressus lusitanica). Organic matter favours the biomass yield of Kikuyu. Although there is no difference in dry matter production between 30 and 60 days of cutting, CP production is higher at 30 (p<0.05). The highest DM production per day is obtained at 45 days, generating a higher biomass accumulation of 21 186.9 kg DM ha-¹. yr-¹. The consideration of Pennisetum clandestinum for dairy cattle is viable, taking into account that the possibility of implementing a plant improvement programme on this species is open, aimed at increasing the composition of the diet in high-production cows due to its high yield and good chemical composition in highland conditions.This work was financed with resources from Project CUI 2432072: 'Mejoramiento de la disponibilidad de material genético de ganado bovino con alto valor a nivel nacional. 7 departamentos' of the Ministry of Agrarian Development and Irrigation - Peru. To the Faculty of Livestock Science Engineering of the National University of Cajamarca Peru authorities for providing the facilities to execute the research. To Cristian Portal Mendo for the Laboratory analysis
Development and application of microsatellite markers for genetic diversity assessment and construction of a core collection of Myrciaria dubia (Kunth) Mcvaugh germplasm from the peruvian Amazon
No full textThe Amazonian shrub Myrciaria dubia (camu-camu) produces vitamin C-rich fruits of growing commercial interest. However, sustainable utilization requires assessment and protection of the genetic diversity of the available germplasm. This study aimed to develop and apply microsatellite markers to assess genetic diversity and construct a core collection of M. dubia germplasm from the Peruvian Amazon. Sixteen polymorphic microsatellite loci were developed using an enrichment approach. The evaluation of 336 genotypes from 43 accessions of the germplasm bank, originating from eight river basins, was conducted using these newly developed markers. Genetic diversity parameters, including observed and expected heterozygosity, were calculated. Analysis of molecular variance (AMOVA) was performed to assess the distribution of genetic variation within and among accessions and river basins. Bayesian clustering analysis was employed to infer population structure. A core collection was constructed to maximize allelic richness. High genetic diversity was observed, with heterozygosity values ranging from 0.468 to 0.644 (observed) and 0.684 to 0.817 (expected) at the river basin level. AMOVA indicated significant genetic variation within (73–86%) compared to among (14–27%) accessions and river basins. Bayesian clustering detected ten genetic clusters, with several degrees of admixture among river basins, except for the genetically homogeneous Putumayo River basin. A core collection comprising 84 plant genotypes (25% of the full collection) was established, representing 90.82% of the overall allelic diversity. These results have important implications for M. dubia conservation strategies and breeding programs, in demonstrating a need for genetic connectivity between populations but preserving unique genetic resources in isolated basins. These results validate the expected levels of diversity and population subdivision in a crop and stress the need to secure genetically diverse germplasms, underscoring the importance of thorough genetic characterization for ex situ germplasm management
Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas
No full textLow motility and low sperm concentration are characteristics of alpaca semen. Thus, the intracytoplasmic sperm injection (ICSI) technique represents an alternative to improve the reproductive capacity of the male. However, the effect of post-ICSI activation in alpaca is not yet known. The aim of the present study was to compare the effect of chemical activators on alpaca embryo development after ICSI. Alpaca ovaries were collected from a local slaughterhouse and transported to the laboratory. Category I, II and III oocytes were matured for 30 h at 38.5 °C. After ICSI, injected oocytes were randomly divided and activated as follows: i) 5 μM ionomycin for 5 min, ii) 7% ethanol for 4 min, iii) 5 μM ionomycin for 5 min, window period 3 h plus 7% ethanol for 4 min, iv) 5 μM ionomycin for 5 min, window period 3 h, a second ionomycin treatment for 5 min, followed by 1.9 mM 6-DMAP for 3 h, v) 10 mM SrCl2 for 3 h. Culture was carried out for 5 days in SOFaa at 38.5 °C. The cleavage rate was the lowest in the SrCl2 group, morula development was the lowest in the SrCl2 and without activation groups, and blastocyst stage was not different between groups (P<0.05). The rates with SrCl2 were lower in total embryos produced, whereas in transferable embryos they were lower with 2Io/6-DMAP and with SrCl2 (P<0.05). In conclusion, alpaca oocyte activation is more efficient with ionomycin and ethanol to produce transferable embryos