Repositorio Institucional INIA (Inst. Nacional de Innovacion Agraria)
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    Phenotypic characterization of wild Myrciaria dubia (Kunth) McVaugh ex situ germplasm bank for breeding, conservation, and sustainable development in the Peruvian Amazon

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    Introduction: Myrciaria dubia “camu-camu” is an economically important Amazonian fruit shrub known for its exceptionally high vitamin C content. Despite its commercial value, comprehensive phenotypic characterization of available genetic resources remains limited, hindering breeding programs and conservation strategies. This study aimed to characterize the phenotypic diversity of wild accessions maintained in a 36-year-old ex situ germplasm bank, one of the most comprehensive M. dubia collections globally, to provide baseline data for genetic improvement and conservation. Methods: We evaluated 43 wild accessions systematically collected from eight major hydrographic basins in the Loreto region using a stratified sampling approach to capture maximum ecological diversity and maintained in an ex situ germplasm bank established in 1988 at the National Institute of Agrarian Innovation in Loreto, Peru. Twenty-three quantitative and six qualitative morphological descriptors were assessed using standardized protocols specifically developed for M. dubia, emphasizing commercially important descriptors including fruit weight, pulp content, and seed characteristics. Statistical analyses encompassed univariate variability assessment, bivariate correlations, and multivariate classification through hierarchical clustering and principal component analysis. Results: Phenotypic characterization revealed moderate overall variability with coefficient of variation averaging 17.4%, with reproductive descriptors showing greater variation than vegetative traits. Fruit and seed descriptors exhibited the highest variability exceeding 20%, while qualitative descriptors showed limited diversity with Shannon Index of 0.823. Multivariate analysis identified four distinct phenetic groups with no significant correlation to geographic origin (Mantel test, p=0.4034). Principal component analysis revealed fruit-related descriptors as primary drivers of phenotypic differentiation, explaining 57.1% of observed variability. Three accessions from phenetic group 3 (PER1000416, PER1000423, and PER1000411) demonstrated superior trait combinations: fruit weight exceeding 13 g, pulp content above 75%, and reduced seed count below 2.5 seeds per fruit. Conclusion: The moderate phenotypic variability observed reflects the natural distribution patterns and limited domestication history of the species. This comprehensive characterization provides essential baseline data and a foundation for targeted breeding programs, conservation strategies, and sustainable production systems supporting development while preserving the genetic diversity in the Peruvian AmazonThe author(s) declare financial support was received for the research, and/or publication of this article. This research was supported by the National Council for Science, Technology, and Technological Innovation (CONCYTEC) through the PROCIENCIA program under the Basic Research Projects funding initiative “E041-2024-03” (Contract Number PE501088786-2024-PROCIENCIA). We also acknowledge the Universidad Nacional de la Amazońıa Peruana (UNAP) for institutional support through the approval of research projects under Resolució n Rectoral N° 0449-2024-UNAP. Additional upport was provided by the “Conservation and Sustainable Use of Genetic Resources of the National Germplasm Bank of INIA Maintained Under Ex Situ Conditions” project (Budget Program 0121)

    Synergistic effects of microbial bioinoculants on root development of purple corn (Zea mays L.) varieties

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    El maíz morado (Zea mays L.), originario de los Andes peruanos, destaca por su alto contenido de antocianinas, lo que le otorga un valor nutracéutico creciente en mercados globales. Sin embargo, su cultivo en zonas altoandinas suele depender del uso intensivo de fertilizantes sintéticos, comprometiendo la sostenibilidad del suelo. Este estudio evaluó como la bioinoculación individual y combinada con Azospirillum brasilense y Glomus iranicum var. tenuihypharum influyen en caracteres morfofisiológicos radicales en cinco variedades contrastantes de maíz morado, cultivadas en la región de Ayacucho (2735 m s.n.m.). Se aplicó un diseño de bloques completos al azar con cuatro tratamientos de bioinoculación. Se midieron peso seco, longitud, volumen, diámetro y número de ramificaciones radiculares. La coinoculación microbiana generó diferencias altamente significativas (p < 0.0001) en todos los parámetros evaluados respecto al control. En particular, duplicó el volumen radical (28,0 ± 0,41 cm³), triplicó el peso seco (180,29 ± 8,51 g) y mejoró notablemente la longitud (31,81 ± 0,48 cm), el diámetro (128,36 ± 2,3 mm) y el número de ramificaciones (27,28 ± 0,66), superando tanto a las inoculaciones individuales como al control (Tukey, p < 0.05). Los resultados confirman que la coinoculación rizobacteria-micorriza mejora significativamente la arquitectura del sistema radical, potenciando la absorción de nutrientes. Así, la coinoculación representa una estrategia agroecológica eficaz para optimizar el rendimiento del maíz morado y reducir la dependencia de insumos sintéticos en contextos altoandinos

    Reporte Repositorio Institucional del 01 al 30 Abril del 2025

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    Durante el mes de Abril se incorporaron 28 publicaciones técnico científicas, en el Repositorio Institucional del INIA, contando a la fecha con un total de 2584 publicaciones, divididas en comunidades y colecciones. El objetivo de este reporte es mantener actualizados los datos sobre las publicaciones técnico-científicas que vienen siendo incorporadas por el área a cargo de la administración del Repositorio Institucional del INIA

    Phenology, growth and yield of nine common bean (Phaseolus vulgaris l.) lines under agroclimatic conditions of chincha, Peru

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    El frijol común (Phaseolus vulgaris L.) es una de las leguminosas más cultivadas a nivel mundial por su alto valor nutricional y papel en la seguridad alimentaria. En el Perú, su producción en el valle de Chincha es vital para pequeños y medianos agricultores, siendo una fuente clave de ingresos. Sin embargo, su productividad depende de factores agroclimáticos y genéticos. Este estudio evaluó el desempeño de nueve líneas de frijol en el valle de Chincha para identificar materiales con mayor potencial productivo. Se empleó un Diseño en Bloques Completos al Azar (DBCA) con 14 variables evaluadas, aplicándose análisis estadísticos como ANOVA, coeficiente de correlación de Pearson, Análisis de Componentes Principales (ACP) y agrupamiento jerárquico. El ACP explicó el 40 % de la variabilidad total en las dos primeras dimensiones. La primera (23,3 %) estuvo asociada a componentes de rendimiento, mientras que la segunda (16,7 %) se relacionó con la fenología, peso de 100 granos y longitud de vaina. El peso de 100 granos presentó correlación positiva con el largo de vaina (r=0,80) y negativa con los días a madurez de cosecha (r=-0,56). Can D8120 y Arbolito (clúster A) fueron las líneas más productivas (1402,75 y 1511,00 kg·ha⁻¹), con peso intermedio de 100 granos (49,30-51,70 g). Larán Mejorado (clúster B) destacó por su mayor tamaño de grano y rendimiento intermedio. Estos hallazgos son clave para la selección de líneas con alto potencial productivo y su incorporación en programas de fitomejoramiento

    Effects of Glomus iranicum inoculation on growth and nutrient uptake in potatoes associated with broad beans under greenhouse conditions

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    The rising global demand for food, including potatoes, necessitates increased crop production. To achieve higher yields, farmers frequently depend on regular applications of nitrogen and phosphate fertilizers. As people seek more environmentally friendly alternatives, biofertilizers are gaining popularity as a potential replacement for synthetic fertilizers. This study aimed to determine how Glomus iranicum affects the growth of potatoes (Solanum tuberosum L.) and the nutritional value of potato tubers when grown alongside broad beans (Vicia faba L.). An experiment was conducted using potatoes tested at five dosage levels of G. iranicum, ranging from 0 to 4 g, to see its impact on the plants and soil. Inoculation with G. iranicum produced variable results in associated potato and bean crops, with significant effects on some variables. In particular, inoculation with 3 g of G. iranicum produced an increase in plant height (24%), leaf dry weight (90%), and tuber dry weight (57%) of potatoes. Similarly, 4 g of G. iranicum produced an increase in the foliar fresh weight (115%), root length (124%), root fresh weight (159%), and root dry weight (243%) of broad beans compared to no inoculation. These findings suggest that G. iranicum could be a helpful biological tool in Andean crops to improve the productivity of potatoes associated with broad beans. This could potentially reduce the need for chemical fertilizers in these crops.In collaboration with LABSAF teams, who provided the essential infrastructure and equipment, soil laboratory analysis was conducted. We extend our sincere gratitude to Elder Ofelia Pinillos Monge, head of the Plant Protection and Biocontrol Laboratory of the Santa Ana Agriculture Center, for the invaluable use of their facilities in preparing microbial inoculants

    Effectiveness of protected areas in containing the loss of Peruvian Amazonian forests

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    Peruvian Amazonian Forests (PAF), vital for biodiversity, climate, and human well-being, lost 2.92M ha during 2001-2022, mainly due to anthropogenic activities. This prompted strategies to conserve and protect the PAF, such as land use and natural resource restrictions, with natural protected areas (NPAs) being the main strategy. This study evaluated the effectiveness of 41 NPAs in containing deforestation in the PAF by analyzing national spatial data. An Effectiveness Index (EI) was constructed by adding five standardized parameters: (1) the percentage of deforested area (DA%) and (2) its annual rate of change (DAr) between 2000 and 2022 inside each NPA, (3) the difference in DAr between NPAs and their surrounding areas, (4) their corresponding ecoregions, and (5) the entire PAF. In 2000, the DA% was 7.15% of the PAF, increasing to 10.88% in 2022. NPAs showed lower DAr than their surrounding areas and ecoregions, except for five NPAs. Of the 41 NPAs, nine were non-effective (EI≤3), 31 moderately effective (3<EI<4), and only one effective (EI≥4). Indirect-use NPAs (strict with integral protection) were slightly more effective than direct-use NPAs (where sustainable use is allowed). Among national categories, the 11 National Parks, equivalent to IUCN Category II, had the highest average EI (3.414). In general, NPAs have shown moderate effective in containing deforestation, and require risk-specific mitigation strategies, especially in NPAs with low DA% but high DAr. Finally, a quantitative and systematic assessment tool is provided, which can improve the formulation of strategies to mitigate deforestation and preserve crucial ecosystem services in PAF

    Reporte de Repositorio Institucional del 01 al 31 de Enero 2025

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    Durante el mes de enero se incorporaron 06 publicaciones técnico científicas, en el Repositorio Institucional del INIA, contando a la fecha con un total de 2495 publicaciones, divididas en comunidades y colecciones. El objetivo de este reporte es mantener actualizados los datos sobre las publicaciones técnico-científicas que vienen siendo incorporadas por el área a cargo de la administración del Repositorio Institucional del INIA

    Efeito do efluente líquido de suínos no crescimento inicial do milho forrageiro em condições de estufa

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    The use of organic effluents from livestock farming as biofertilizers for agriculture represents a viable solution for the management of swine waste. The research aimed to evaluate the effect of swine liquid effluent on the growth of hybrid corn for forage purposes. A pot experiment was designed to determine the effect of liquid effluent applications of treatments 21 days after planting. The biofertilizer treatments applied to the irrigation water in the pots were 0.00 (control), 9.45, 18.90, 28.35, 37.80 and 47.25 m3 ha-1. Applications of 37.80 and 47.25 m3 ha-1 improved stem diameter, foliage, and total fresh weight. For root fresh weight and root volume, the highest values occurred with applications of 18.90 to 47.25 m3 ha-1. The nutrient content (N, P, K) of corn treated with swine liquid effluent showed an incremental trend in foliage and roots, as well as the absorption of macronutrients due to the effect of the treatments. Liquid swine effluent showed a promising role as an organic fertilizer for the cultivation of maize for forage purposes, promoting higher biomass production and macronutrient uptake

    Influence of reproductive season and testicular volume on seminal parameters of alpacas (Vicugna pacos)

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    The male alpaca plays an important role in the reproductive process and, consequently, in genetic improvement. South American camelids (SACs) have a well-defined reproductive season, particularly in the Andean region. The objective was to evaluate the influence of reproductive and non-reproductive seasons, as well as testicular volume, on seminal parameters in alpacas. Nine healthy male alpacas, over 4 years old, were selected for this experiment. Semen samples were taken every two days using an artificial vagina incorporated into a dummy. The collected were in the reproductive (November to April) and non-reproductive (May to October) seasons, from breeders with testicular volumes that range between 63–70 cm3 and 83–94 cm3. The total number of ejaculates collected was 72 per season, with 8 samples collected from each male. Macroscopic aspects such as color, volume (ml), thread formation (cm) and foam (cm) were evaluated, in addition to microscopic aspects such as sperm motility (%), viability (%) concentration (sperm/ml) and total number of sperm. The results were analyzed with the Mann–Whitney non-parametric test, using the R 3.4.3 software, considering P < 0.05 as the significance level. A decrease in thread formation was observed in semen collected during the reproductive season compared to the non-reproductive season (P < 0.05). On the other hand, sperm motility and viability in semen collected during the reproductive period was higher (p < 0.05). Regarding testicular volume, alpacas with larger testicular volume had greater ejaculate volume, sperm concentration and total number of sperm (p < 0.05). Based on these results, it is possible to conclude that the semen of alpaca breeders is influenced by the season, and that testicular volume can predict their reproductive capacity, being a parameter to be considered when selecting the best breeding male

    Climate change and tree cover loss affect the habitat suitability of Cedrela angustifolia evaluating climate vulnerability and conservation in Andean montane forests

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    Background. Because of illegal logging, habitat fragmentation, and high value timber Andean montane forest Cedrela species (such as Cedrela angustifolia), is endangered in Central and South America. Studying the effects of climate change and tree cover loss on the distribution of C. angustifolia will help us to understand the climatic and ecological sensitivity of this species and suggest conservation and restoration strategies. Methods. Using ecological niche modeling with two algorithms (maximum entropy (MaxEnt) and Random Forest) under the ecological niche conservatism approach, we generated 16,920 models with different combinations of variables and parameters. We identified suitable areas for C. angustifolia trees under present and future climate scenarios (2040, 2070, and 2100 with SSP 3-7.0 and SSP 5-8.5), tree cover loss, and variables linked to soil and topography. Results. Our results demonstrated 10 environmental variables with high percentage contributions and permutation importance; for example, precipitation seasonality exhibited the highest contribution to the current and future distribution of Cedrela angustifolia. The potential present distribution was estimated as 13,080 km2 with tree cover loss and 16,148.5 km2 without tree cover loss. From 2040 to 2100 the species distribution will decrease (from 22.16% to 36.88% with tree cover loss variation). The results indicated that Bolivia displayed higher habitat suitability than Ecuador, Peru, and Argentina. Finally, we recommend developing conservation management strategies that consider both protected and unprotected areas as well as the impact of land-use changes to improve the persistence of C. angustifolia in the future

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    Repositorio Institucional INIA (Inst. Nacional de Innovacion Agraria)
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