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No full textNot AvailablePhytic acid (PA) acts as a storehouse for the majority of the mineral phosphorous (P) in
maize; ~80% of the total P stored as phytate P is not available to monogastric animals and thereby
causes eutrophication. In addition, phytic acid chelates positively charged minerals making them
unavailable in the diet. The mutant lpa1-1 allele reduces PA more than the wild-type LPA1 allele.
Further, mutant gene opaque2 (o2) enhances lysine and tryptophan and crtRB1 enhances provitamin-A
(proA) more than wild-type O2 and CRTRB1 alleles, respectively. So far, the expression pattern of
the mutant lpa1-1 allele has not been analysed in maize genotypes rich in lysine, tryptophan and
proA. Here, we analysed the expression pattern of wild and mutant alleles of LPA1, O2 and CRTRB1
genes in inbreds with (i) mutant lpa1-1, o2 and crtRB1 alleles, (ii) wild-type LPA1 allele and mutant
o2 and crtRB1 alleles and (iii) wild-type LPA1, O2 and CRTRB1 alleles at 15, 30 and 45 days after
pollination (DAP). The average reduction of PA/total phosphorous (TP) in lpa1-1 mutant inbreds
was 29.30% over wild-type LPA1 allele. The o2 and crtRB1-based inbreds possessed ~two-fold higher
amounts of lysine and tryptophan, and four-fold higher amounts of proA compared to wild-type
alleles. The transcript levels of lpa1-1, o2 and crtRB1 genes in lpa1-1-based inbreds were significantly
lower than their wild-type versions across kernel development. The lpa1-1, o2 and crtRB1 genes
reached their highest peak at 15 DAP. The correlation of transcript levels of lpa1-1 was positive for
PA/TP (r = 0.980), whereas it was negative with inorganic phosphorous (iP) (r = 0.950). The o2 and
crtRB1 transcripts showed negative correlations with lysine (r = 0.887) and tryptophan (r = 0.893),
and proA (r = 0.940), respectively. This is the first comprehensive study on lpa1-1 expression in the
maize inbreds during different kernel development stages. The information generated here offers
great potential for comprehending the dynamics of phytic acid regulation in maize.Not Availabl
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No full textNot AvailableThe material for the study consisted of three stable CMS lines viz., CMS 103A, CMS104A, and CMS 38A
as female lines and 50 new inbred lines as testers. All three CMS lines and 50 inbred lines were crossed in a Line × Tester fashion and the resultant 150 hybrids were evaluated for fertility restoration. Among common restorers across all three CMS lines, RCR-20-8 recorded the highest gca for seed yield, oil content, and plant height, followed by RCR 20-19 for head diameter and seed yield; RCR-20-13 for volume weight and early flowering. These lines can be used as testers for developing hybrids and also for the development of new tester lines with a combination of desirable characters.Not Availabl
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No full textNot AvailableThe rice root-knot nematode, Meloidogyne graminicola, is one of the most important plant-parasitic nematodes present in various production systems in all major rice-growing areas of the world. The vermiform second-stage juvenile (J2) is the infective stage and may be found either inside the roots or in the soil. When in the soil, they penetrate the plant root in the elongation zone. J2s migrate intercellularly through the cortex to the vascular cylinder where they induce the formation of specialized feeding cells, called giant cells, start feeding, and become swollen and sedentary. In contrast to other root-knot nematodes, females deposit their eggs in a gelatinous matrix inside the roots. Hyperplasia and hypertrophy of surrounding cells cause the formation of galls or root-knots, mainly at the root tips and with a characteristic hook-like shape. M. graminicola has a relatively short life cycle on rice (2 to 4 weeks) and can cause 20–33% yield losses in susceptible cultivars.ICA
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No full text2OGDs are known to catalyze a plethora of reactions and also confer resistance to different pathogens in various crops, but not much is known about the 2OGD in Musa species. Through a comprehensive genome-wide analysis, 133 and 122 potential 2OGDs were systematically identified and categorized from the A and B genomes of banana, respectively. Real time expression of dmr6 and dlo1 genes showed positive correlation with transcriptome data upon Foc race1 and TR4 infection and examination of expression pattern of Macma4_04_g22670 (Ma04_g20880) and Macma4_02_g13590 (Ma02_g12040) genes revealed their involvement in Foc race1 and TR4 infections, respectively. Further the expression profile of 2OGDs, specifically Macma4_04_g25310 (Ma04_g23390), Macma4_08_g11980 (Ma08_g12090) and Macma4_04_g38910 (Ma04_g36640) shows that they may play a significant role as a susceptibility factor, particularly against P. eumusae and P. coffeae, implying that they can be exploited as a candidate gene for editing in developing resistant cultivars against these diseases. In summary, our findings contribute to a deeper comprehension of the evolutionary and functional aspects of 2OGDs in Musa spp. Furthermore, they highlight the substantial functions of these family constituents in the progression of diseases. These insights hold significance in the context of enhancing the genetic makeup of bananas to attain extended and more durable resistance against pathogens.Bananas are an important staple food and cash crop, but they are vulnerable to a variety of pests and diseases that substantially reduce yield and quality. Banana diseases are challenging to control and necessitate an integrated strategy, and development of resistant cultivars is one of the effective ways of managing diseases. Lasting disease resistance is the main goal in crop improvement and resistance mediated by a single resistant (R) gene mostly lack durability. However, long-term resistance can be obtained by inactivating susceptibility factors (S), which facilitate pathogen infection and proliferation. Identification and inactivation of susceptibility factors against the major pathogens like Fusarium oxysporum f. sp. cubense (Foc), Pseudocercospora eumusae and Pratylenchus coffeae in banana will be an effective way in developing banana varieties with more durable resistance. Downy mildew resistance 6 (DMR6) and DMR-like oxygenases (DLO1) are one such susceptibility factors and they belong to 2-oxoglutarate Fe(II) dependent oxygenases (2OGD) superfamily.Not Availabl
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No full textChapter 4 of Training manual “Advances in seafood processing and waste utilization ”Not AvailableNot Availabl
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No full textChapter 12 of Training manual “Advances in seafood processing and waste utilization ”Not AvailableNot Availabl
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No full textChapter 1 of Training manual “In-plant training under student ready program”Not AvailableNot Availabl
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No full textChapter 8 of Training manual “In-plant training under student ready program”Not AvailableNot Availabl
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No full textChapter 21 of Training manual “In-plant training under student ready program”Not AvailableNot Availabl