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GYRB – POLYMERASE CHAIN REACTION AND HISTOPATHOLOGIC CHARACTERISTIC FIGURE POTENTIAL FOR DETERMINING DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS
Full text linkBackground: TB lymphadenitis is still a problem that needs serious treatment. In Indonesia, it was reported that 53% of TB cases were extrapulmonary tuberculosis, with the most cases being Lymphadenitis TB, 11.6%. In children, 43% of extrapulmonary tuberculosis cases are TB lymphadenitis. Diagnosis is quite difficult; a method of determining the diagnosis and appropriate comprehensive treatment is required in managing TB Lymphadenitis.
Materials and Methods: In this study, 15 fine needle aspiration biopsy aspirate samples were subjected to molecular examination using the gyrB–polymerase chain reaction method and histopathological observations using the smear method with hematoxylin-eosin staining. Observation of preparations using a microscope with a magnification of 200x.
Results: The histopathological characteristics of the fine needle aspiration biopsy aspirate showed positive results in 4 out of 15 samples, with epithelioid cells arranged in a characteristic granuloma structure, necrotic debris was visible, and cells joined together to form multinucleated giant cells as an inflammatory response to complex infection. In this study, 6 out of 15 (40%) were detected to be positive in the diagnosis based on molecular detection using a specific target gene gyrB - polymerase chain reaction.
Conclusion: Characteristic features on histopathological examination associated with gyrB - positive polymerase chain reaction on lymphadenitis fine needle aspiration biopsy aspirate samples can be used as a determinant diagnosis of tuberculous lymphadenitis
OBESITY AND OUTCOME AMONG PATIENTS HOSPITALIZED SARS-COV-2: A HOSPITAL-BASED STUDY IN INDONESIA: Obesity and Outcome among Patients Hospitalized SARS-CoV-2
No full textBackground: Patients with obesity who have SARS-CoV-2 are at significant risk for developing serious clinical problems that need intensive care and have a bad prognosis.
Aim: The aim of this study was to determine obesity and outcome among patients hospitalized SARS-CoV-2 at Indonesia's national referral hospital.
Methods: This study used a retrospective cohort. The research sample was SARS-CoV-2 patients who were treated by pulmonary specialists in the intensive room of Dr. M. Djamil Hospital Padang. The number of samples in this study was 106 subjects. Data analysis was performed using the Chi-square test, Kaplan-Meier, and Cox regression. P < 0.05 was significant, and the data were analyzed using the SPSS version 21.0 program.
Results: The results of this study found obesity was associated with the outcome of SARS-CoV-2 patients treated at Indonesia's national referral hospital (p<0.05, OR=3.55 (95% CI 1.44-8.71)). The mortality rate among patients hospitalized with SARS-CoV-2 at Indonesia's national referral hospital with obesity was higher than non-obese, namely 82.7% and 57.4%. The length of stay in patients with obesity was also shorter, namely 12 days compared to 19 days in non-obese.
Conclusion: There was an association between obesity with mortality of SARS-CoV-2 patients in a national referral hospital Indonesia. This study can provide input in the therapeutic management of patients with obesity so as to reduce the poor prognosis.
OVERVIEW OF COVID-19 CASES IN PREGNANCY AT THE HOSPITAL UNIVERSITAS SUMATERA UTARA, INDONESIA, WHEN THE PANDEMIC STORM HIT IN THE 2020-2022 PERIOD. : COVID-19 Cases In Pregnancy
No full textBackground: COVID-19 (Coronavirus 2019) is caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome-Coronavirus-2), an acute infectious disease primarily affecting the respiratory system. Data on COVID-19 exposure during pregnancy and issues associated with COVID-19 during pregnancy remain limited. This research aimed to determine the number of pregnant women infected by COVID-19, laboratory test findings of pregnant women related to COVID-19 infection, infant outcome from mother with or without COVID-19 infection and referential status for COVID-19 and non-COVID-19 pregnant women at the USU Hospital during the 2020–2022 period.
Materials and Methods: This research was conducted using a descriptive method with a cross-sectional study approach using a non-probability sampling technique by collecting secondary data from COVID-19 and non-COVID-19 pregnant women at the USU Hospital during the 2020-2022 period, where 112 samples were obtained.
Results: The majority of COVID-19 pregnant women and non-COVID-19 pregnant women have been identified sequentially based on Hb (11.6%-decreased vs 79.5%-normal); Ht (11.6%-decreased vs 76.8%-increased); leukocytes (11.6%-increased vs 83%-normal); thrombocytes (8.9%-normal vs 86.6%-normal); PT (9.8%-normal vs 50.9%-normal); APTT (11.6%-normal vs 87.5%-normal); D-dimer (11.6%-long vs 56.3%-long); procalcitonin (7.1%-increased vs 87.5%-normal); NLR (8%-increased vs 82.1%-normal); CRP (12.5%-increased vs 87.5%-normal) and all of the baby outcomes were non-COVID-19 and the majority of pregnant women were not referred.
Conclusion: Based on the data in this study, the majority of pregnant women and babies at the USU Hospital during the 2020–2022 period were non-COVID-19 positive and with non-referral status. Laboratory findings of COVID-19 in pregnancy significantly reveals abnormalities
STRUCTURE-ACTIVITY-RELATIONSHIP OF THE POLYPHENOLS INHIBITION OF α-AMYLASE AND α-GLUCOSIDASE
Full text linkBackground: Diabetes mellitus (DM) is a serious public health challenge, projected by WHO to be one of the 7 leading cause of death by 2030. Medicinal plants have been demonstrated to be useful in DM local management because of polyphenols present in these plants. For an alternative treatment approach especially with polyphenols-rich herbs, knowledge of comparative efficacy of the polyphenols will lead to enhanced therapy especially in postprandial hyperglyceamic control.
Materials and Methods:Vegetative parts of Anacardium occidentale, Abelmoschus ecsulentus and Ceiba pentandra, prominent in the local management of DM were identified, collected and subjected to alcoholic extraction. From the crude extracts were isolated agathisflavone, quercetin 3-O-glucoside, quercetin 3-O-diglycoside, mangiferin, isomangiferin and pentagalloyl glucose, belonging to flavonoid, xanthones and tannins structural classes. These polyphenols were evaluated for their potentials to inhibit both α-glucosidase and α-amylase. Physicochemical parameters of the polyphenols were evaluated and molecular docking experiments were carried out to gain insight into the observed inhibitory activity.
Results: quercetin 3-O-glucosidewas the most potent of the polyphenols against the two enzymes. Increase in the number of phenolic hydroxyl group did not increase the inhibitory activity and neither computation of the binding energies with the enzymes nor physicochemical parameters of the polyphenols could explain the observed inhibitory activity against the enzymes, across the structural classes. Thus, only the bioassay against the enzymes α-glucosidase and α-amylase correlated well with the use of the plants in treating diabetic mellitus
Conclusion: Medicinal plants rich in quercetin 3-O-glycoside may have better treatment outcomes in postprandial hyperglycaemia control
BIOMOLECULAR ACTIVITY OF CRYPTOCOCCUS DURING CRYPTOCOCCOSIS: FOCUS ON REVIEW OF MOLECULAR INTERACTION CRYPTOCOCCUS WITH HUMAN IMMUNE SYSTEM AND BLOOD-BRAIN BARRIER
Full text linkGlobal mycosis is still a problem. One of these is the cryptococcal disease. A systemic mycosis brought on by Cryptococcus is called cryptococcosis. Host immunological conditions influence infection with Cryptococcosis. When environmental spores are inhaled by the host, the spores get to the lungs, an infection is created. Alveolar macrophages and other immune cells recognize Cryptococcus in the lung. The initial line of defense against pathogens in the phagolysosome is provided by alveolar macrophages found in the lungs. When the immune system is weak, Cryptococcus uses the evasion system as a molecular interaction with the immune system and persists in the lungs without causing any symptoms such as Factor Transcription, Cell masking, N-glycan structure, Extracellular molecule, and Antioxidant system. The evasion mechanism protects and makes Cryptococcus disseminate throughout the other organs, especially CNS. If Cryptococcus escapes against the host immune system, it will disseminate to other organs, especially Cerebrospinal System by Three mechanisms. There are Trojan Horse, Paracellular, and Transcellular interactions with Blood-Brain Barrier. Disease severity is determined by the Interaction between the host’s immune system and the fungu
BACKSLIDING ON CHILDHOOD IMMUNIZATIONS DUE TO ONGOING COVID-19 PANDEMIC: A RETROSPECTIVE STUDY IN BANADIR REGION, SOMALIA
Full text linkBackground: SARS-CoV-2 has resulted in a global public health crisis. During the pandemic, considerable delay was observed making it impossible for some children to receive their due vaccines on time. Like most resource-poor countries, COVID-19 pandemic is thought to have a negative impact on Somalia’s immunization coverage.
Materials and methods: This study aimed to assess the impact of the COVID-19 pandemic on routine childhood immunization coverage in Somalia. A retrospective comparative cross-sectional approach was employed to investigate the number of under-5-year children who got their immunization from the two major mother and child hospital, (Banadir and SOS in Mogadishu, Somalia from October 2019 to December 2020. To do this, a total of 112, 060 data relating to the routine childhood immunization (measles, polio, whooping cough, hepatitis B, pneumonia, and tuberculosis) were collected from the monthly immunization report-data from the two hospitals.
Results: The results showed that all the vaccines except birth vaccines have remarkably dropped with Penta-3 (27%), Penta-2 (11%), measles (10%) and Penta-1 (8%) respectively. However, the birth vaccines (BCG and Polio 0) were not affected as observed in this study. The reduction in children immunization rate in Somalia may be a combination of many other factors, we however recognize that the COVID-19 pandemic may have contributed significantly to this outcome.
Conclusion: The government needed to take proactive measures to encourage parents to present their children for immunizations, including increasing community concerning the importance of these routine childhood immunizations despite the ongoing COVID-19 pandemics
REACTOGENICITY OF HETEROLOGOUS MRNA-BASED COVID-19 VACCINE BOOSTER IN YOUNG ADULTS IN INDONESIA- A SHORT COMMUNICATION.: COVID-19 vaccine booster reactogenicity in young adults
No full textBackground: Heterologous priming with the inactivated SARS-CoV-2 vaccine (CoronaVac) and boosting with mRNA-based COVID-19 vaccine (Moderna or Pfizer) is currently recommended in Indonesia. The reactogenicity data of these heterologous vaccine regimens are not entirely available, particularly in young adults.The present study, therefore, aimed to evaluate the solicited local and systemic reactions in the first seven days post-vaccination either with Moderna or Pfizer vaccine among previous recipients of two doses of CoronaVac.
Materials and Methods: An electronic-based cross-sectional study was conducted among medical students at the Pelita Harapan University, Banten, Indonesia, who received mRNA-based COVID-19 vaccine following two doses of CoronaVac. Samples were collected using a cluster sampling technique. Comparison between groups was performed by Fisher’s exact test.
Results: A total of 72 participants, with 23 (32%) of which received the Moderna vaccine and 49 (68%) received the Pfizer vaccine, were included in this study. The median age of participants was 21 (IQR 19-22) years old. The most common local and systemic events for mRNA-based COVID-19 vaccines were injection site pain, fever, headache, fatigue, myalgia, and arthralgia. Solicited local and systemic reactions were reported more frequently in Moderna recipients than Pfizer recipients. Most local and systemic reactions were graded as mild to moderate and did not lead to hospitalization.
Conclusions: The reactogenicity of the heterologous prime-boost with CoronaVac and mRNA-based COVID-19 vaccine booster among young adults is reassuring, and no unexpected concerns were identified
AN ESSENTIAL TOOL TO BE OPTIMIZED: SYNDROMIC MANAGEMENT OF VAGINAL DISCHARGE IN HAITI
Full text linkBackground: Haiti, like many low-income countries in crisis, has limited resources for etiologic diagnosis of vaginal discharge. As such, we sought to characterize variability in diagnoses of women presenting with vaginal discharge syndrome, with the goal to improve standardization of syndromic management.
Materials and Methods: Participants aged 18 years and older endorsing vaginitis, or dysuria were recruited at Jerusalem Clinic over two, one-week periods in April 2018 and July 2019. We calculated Spearman rank correlations among history, exam findings, and diagnoses based on clinical presentation, to understand presentation groupings and their management.
Results: Among 98 women, median age was 33.5 years, and most frequent symptoms were: vaginal discharge (97%), vaginal itch (73%), and/or suprapubic pain (68%). Most common physical exam findings were vaginal discharge (86%), suprapubic/lower quadrant tenderness (29%), cervical motion tenderness (24%), and cervical erythema (20%). Most symptoms and physical exam findings were weakly correlated with each other. Nearly one-third (31%) were diagnosed with normal physiologic vaginal discharge or no diagnosis, followed by Bacterial vaginosis (31%), vulvovaginal candidiasis (15%), cervicitis/PID (13%), and STI (7%). No reported symptoms strongly differentiated diagnostic categories. Diagnoses varied considerably by exam findings.
Conclusions: The weak correlations between symptoms, exam findings, and diagnoses could represent variability in assessment. In the absence of reliable and accessible laboratory testing, the importance of standardizing syndromic management becomes increasingly relevant. Results from our study support the utility of speculum examination and more standardized documentation of physical exam findings. Next steps include the development of local algorithms to promote standardization of treatment of vaginal discharge syndrome
STRYCHNOS SPINOSA LAM: COMPREHENSIVE REVIEW ON ITS MEDICINAL AND NUTRITIONAL USES.
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Background: Most of the people living in developing countries rely on local remedies from traditional medicines to take care of their healthcare needs. In recent years, the use of medicinal plants increased considerably. Consequently, studies on medicinal plants are necessary to produce more effective drugs with fewer side-effects. This work aims to compile the dispersal data on Strychnos spinosa Lam., a medicinal plant with great therapeutic potential in traditional medicine, on its ethnobotanical uses, chemical compounds, as well as its pharmacological and nutritional effects.
Material and Methods: To achieve the aforementioned aim, electronic databases such as Pubmed, Science Direct, Google scholar, Scopus and SciFinder were scanned.
Results: Literature survey revealed that S. spinosa is used in the treatment of microbial infections, malaria, trypanosomiasis, intestinal worms, tuberculosis, infertility, gastrointestinal disorders, snake bite, hypertension, and diabetes mellitus among other ailments. The plant is also used as food material. S. spinosa contains many chemical compounds, such as alkaloids, terpenes, sterols, fatty acids, flavonoids, saponin, and others. The fruits are a source of important nutrients such as carbohydrates, sugars, proteins, vitamins and fibres. Experimental studies have shown numerous pharmacological properties, including antimicrobial, antiplasmodial, anti-trypanosomal, anti-leishmanial, anti-nematicidal, anti-inflammatory, anti-oxidant, antidiabetic and other activities.
Conclusion: This review article provides comprehensive information on S. spinosa and its various extracts. Due to the promising effects of the plant extracts on various diseases and its nutritional values, there is a need for toxicity studies and clinical trials to assess the effects of its identified bioactive compounds