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TRADITIONAL CHINESE MEDICINE COMBINED WITH HORMONE THERAPY TO TREAT PREMATURE OVARIAN FAILURE: A META-ANALYSIS OF RANDOMIZED CONTROLLED TRIALS
Get PDFBackground: This meta-analysis aimed to provide critically estimated evidence for the advantages and disadvantages of Chinese herbal medicines used for premature ovarian failure (POF), which could provide suggestions for rational treatments. Materials and Methods: The databases searched included MEDLINE, EMBASE, CNKI, VIP, China Dissertation Database, China Important Conference Papers Database, and online clinical trial registry websites. Published and unpublished randomized controlled trials of traditional Chinese medicine (TCM) combined with hormone therapy (HT) and HT alone for POF were assessed up to December 30, 2015. Two authors extracted data and assessed trial quality independently using Cochrane systematic review methods. Meta-analysis was used to quantitatively describe serum hormone levels and Kupperman scores associated with perimenopause symptoms. Results: Seventeen randomized controlled trials involving 1352 participants were selected. Compared with HT alone, although no significant effects were observed in the levels of luteinizing hormone, therapy with TCM combined with HT compared to HT alone effectively altered serum hormone levels of follicle stimulating hormone (
OXIDATIVE STRESS IN A RAT MODEL OF COTTON SMOKE INHALATION-INDUCED PULMONARY INJURY
Get PDFBackground: Smoke inhalation injury refers to airway and lung parenchyma injury and general chemical damage caused by inhaling toxic gases and substances. The aim of this study was to explore the oxidative stress mechanism of cotton smoke inhalation-induced pulmonary injury in a rat model. Materials and Methods: Eighteen male Sprague-Dawley rats were randomly divided into control group, 6 h group, and 24 h group (six rats in each group), which duplicated previous rat cotton smoke-inhalation injury models. Rats in 6 h and 24 h groups were euthanised at 6 h and 24 h after smoke inhalation, respectively. ELISA method was used to detect indicators in the rats’ lung tissue. Quantitative iNOS mRNA and γ-GCS mRNA measurements were performed using a fluorescence PCR method. Results: The concentrations of MDA, NO, iNOS, γ-GCS, iNOS mRNA, and the relative expression of γ-GCS mRNA in the rats’ lung tissues in 6 h and 24 h groups were higher than control group (P < 0.05), and the concentration of NO and relative expressions of iNOS mRNA and γ-GCS mRNA in 24 h group were significantly higher than 6 h group (P < 0.05). The concentrations of GSH in 24 h and 6 h groups were significantly lower than control group (P < 0.05), and that in 24 h group was even significantly lower than 6 h group (P < 0.05). Conclusion: In rats with cotton smoke inhalation-induced pulmonary injury, the increased iNOS mRNA transcription can cause increase of iNOS synthesis and promotion of NO synthesis. The increased γ-GCS mRNA transcription can cause increase of γ-GCS synthesis and but decrease of GSH concentration. The activation of the antioxidant system is insufficient to combat oxidative stress damage. So the oxidant/antioxidant system is imbalanced, leading to gradual aggravation of lung injury
FATIGUE IN PATIENTS WITH SJÖGREN’S SYNDROME AND INTERVENTION OF TRADITIONAL HERBALMEDICINE
Get PDFBackground: Fatigue is the main complaint exiting in patients with primary Sjögren’s Syndrome (pSS) but rarely addressed.
Patients described it as an uncontrollable symptom of lack of energy, which has negative impacts on health related quality of life. To
date, many studies have demonstrated that cytokines, depression, sleep and endocrine disturbance interrelated with pSS-related
fatigue. However, the pathogenesis remains unclear. With a long history, Traditional Chinese Medicine (TCM) as alternative therapy
has become increasingly popular among patients with various kinds of disease, especially in pSS. Based on the unique principle of
therapy, practitioners have achieved a satisfactory effect on relieving disease-related symptoms with Chinese Herbal Medicine
(CHM).
Materials and Methods: In this article, we succinctly reviewed the highly correlated factors to pSS-related fatigue from the
standpoint of western medicine. Then, from TCM perspective, we illustrated that theoretic mechanisms lead to fatigue in patients
with pSS.
Results: According to the theory of TCM, we concluded that CHM as complementary and alternative medicines are attractive
options to alleviate pSS-related fatigue.
Conclusion: In clinic, physicians should remember to inquire whether their patients are worn out easily. Combination of
Yin-tonifying and Qi-tonifying CHM may be the optimal options to pSS-related fatigue
METABOLIC EFFECTS OF TULBAGHIA VIOLACEA HARV. IN A DIABETIC MODEL.
Get PDFBackground: Diabetes mellitus (DM) is a cluster of metabolic diseases with chronic hyperglycemia as a defining feature, associated
with long-term organ damage and dysfunction. In this study we examined the effect of Tulbaghia violacea rhizome methanolic
extract on blood glucose and lipids in normal and streptozotocin-induced diabetic rats.
Materials and Methods: Male Wistar rats (250-300g) were injected intraperitoneally (i.p.) with streptozotocin (60mg/kg body
weight) to induce diabetes; or provided with distilled water for the control (CON) (3 ml/kg/b.w.) animals and treatment with TVL
(60mg/kg.b.w). The rats were divided into 5 groups of 6 animals per group as follows: Non-diabetic control (NDC)-received
distilled water (3ml/kg.b.w), Non-diabetic TVL (ND+TVL)-received TVL (60mh/kg b.w), Diabetic control (DC)-received distilled
water (3ml/kg.b.w), Diabetic experimental (D+TVL)-received TVL (60mg/kg.b.w), Diabetic experimental (D+M)-received
Metformin (250 mg/kg.b.w). All doses were administered daily via oral gavage.
Results: TVL-treated animals showed reduced blood glucose, increased plasma insulin, reduced plasma TG, TC, VLDL and
increased HDL. Furthermore we found decreased thiobarbituric acid reactive substances (TBARS) and increased superoxide
dismutase (SOD) activity and nitric oxide significantly. Regarding renal parameters we found increased blood urea nitrogen (BUN),
and improved renal morphology in TVL-treated animals.
Conclusion: Tulbaghia violacea has a hypoglycaemic effect which could due to its effects on multiple pathways of the
hyperglycemic process. Further work is needed to identify the mechanism of its antidiabetic effect
TRADITIONAL CHINESE MEDICINE TONGXINLUO PROTECTS MICROVASCULAR FROM ARTERIOLE SPASM INDUCED BY NOREPINEPHRINE IN RATS MESENTERY
Get PDFBackground: Arteriole spasm (AS) served as a common pathogenesis of many diseases. Tongxinluo (TXL), a traditional Chinese
formula, has been proved to benefit patients with diseases associated with AS. However, the effect of TXL on AS has not been
elucidated, especially on the hemorheology of microcirculation. The aim of this study was to assess the protective function of TXL
against AS on microcirculation in rats’ mesentery.
Materials and Methods: Arteriole spasm model was established by local administration of norepinephrine in rats’ mesentery. Rats
were randomized divided into five groups: sham group (local administration of normal saline), arteriole spasm group, TXL0.4+AS
group, TXL0.8+AS group, TXL1.6+AS group. Drugs were administered orally daily for 7 days before modeling. Microcirculation
was observed using an upright microscope and recorded to study the hemorheology changes, including spasm time, diameter
changes, and arteriole blood flow. Mast cell degranulation was identified by vital staining with topical application of 0.1% toluidine
blue. The serum samples were collected for detect of NO and ET-1.
Results: Pre-administration of TXL markedly decreased arteriole spasm time, and indicated a dose-dependent effect.
Pre-administration of TXL also indicates dose-dependent effect to maintain arteriole diameter in the presence of AS. Pretreatment
with TXL significantly inhibited mast cell degranulation induced by AS, TXL also exert great beneficial protection on arteriole
blood flow against AS. The serum level of NO and ET-1 had no obvious changes among each groups.
Conclusion: AS caused microvascular dysfunction in rats’ mesentery. TXL had a beneficial effect against AS in the perspective of
protecting microvascular function, indicated that TXL fight against AS through regulation on microcirculation
THE BIOLOGICAL ACTIVITIES OF HYPERICUM PERFORATUM L.
Get PDFBackground: Mastitis reduces milk yield and alters milk composition. Antibiotics are widely used in the treatment of the disease. However, this widespread use of antibiotics causes both antibiotic residues in milks and antibiotic resistance developed in bacteria. Today’s researches are focused on discovering and using new antibiotics against bacteria.
Objective: The aim of this work was to investigate the antibacterial effects of Hypericum perforatum L. extracts against mastitis pathogens, and its other biological activities.
Material and Methods: The extracts were tested by disc diffusion assay. The MIC was evaluated on plant extracts as antibacterial activity. The non-enzymatic antioxidant activity was determined using DPPH as a free radical.
Results: The extract showed maximum inhibition zone against two bacteria (Coagulase-negative staphylococci- 33 and 37; CNS 33 and 37), and the zone was 17 mm. A bacterium (CNS – 22) showed the lowest sensitivity to 812,5 µg /mL concentration. In addition, the extract was tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical for antioxidant activity. As a result, the extract displayed a strong antioxidant activity (trolox equivalent: 0,83 mM).
Conclusion: The extract of Hypericum perforatum have antibacterial, antioxidant and antimutagenic potentials
IMAGING OF BRAIN FUNCTION BASED ON THE ANALYSIS OF FUNCTIONAL CONNECTIVITY - IMAGING ANALYSIS OF BRAIN FUNCTION BY FMRI AFTER ACUPUNCTURE
Get PDFObjective: This Study observed the relevant brain areas activated by acupuncture at the Taichong acupoint (LR3) and analyzed the
functional connectivity among brain areas using resting state functional magnetic resonance imaging (fMRI) to explore the acupoint
specificity of the Taichong acupoint.
Methods: A total of 45 healthy subjects were randomly divided into the Taichong (LR3) group, sham acupuncture group and sham
acupoint group. Subjects received resting state fMRI before acupuncture, after true (sham) acupuncture in each group. Analysis of
changes in connectivity among the brain areas was performed using the brain functional connectivity method.
Results: The right cerebrum temporal lobe was selected as the seed point to analyze the functional connectivity. It had a functional
connectivity with right cerebrum superior frontal gyrus, limbic lobe cingulate gyrus and left cerebrum inferior temporal gyrus (BA
37), inferior parietal lobule compared by before vs. after acupuncture at LR3, and right cerebrum sub-lobar insula and left cerebrum
middle frontal gyrus, medial frontal gyrus compared by true vs. sham acupuncture at LR3, and right cerebrum occipital lobe cuneus,
occipital lobe sub-gyral, parietal lobe precuneus and left cerebellum anterior lobe culmen by acupuncture at LR3 vs. sham acupoint.
Conclusion: Acupuncture at LR3 mainly specifically activated the brain functional network that participates in visual function,
associative function, and emotion cognition, which are similar to the features on LR3 in tradition Chinese medicine. These brain
areas constituted a neural network structure with specific functions that had specific reference values for the interpretation of the
acupoint specificity of the Taichong acupoint
THE THERAPEUTIC EVALUATION AND MECHANISM ON TREATING BRONCHIAL HYPER-RESPONSIVENESS COUGH BY ZIYINQINGRE PRESCRIPTION
Get PDFObjective: Discussing the effects of Ziyinqingre prescription on the level of airway resistance (Rrs), airway response threshold
(Dmin), airway conductance (sGrs) and the level of inflammatory cytokines interleukin-4 (IL-4) and interferon-γ (IFN-γ) of the
bronchial hyper-responsiveness (BHR) cough patients.
Method: 84 subjects diagnosed as BHR were randomly divided into 42 Chinese Traditional medicine group and 42 control group.
The Chinese Traditional Medicine group received Ziyinqingre prescription twice a day and the control group received 10mg
Montelukast Sodium tablets once a day for two weeks. Observe the clinical symptoms improvement and the changes of the level of
the Rrs, Dmin, sGrs and IL-4, IFN-γ.
Results: After receiving the medicine, the symptoms of the Chinese medicine group were obviously alleviated, the outcome was
more satisfied than that of the control group. Compared with the control group, the level of Dmin increased and sGrs level decreased
more obviously (
EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
Get PDFBackground: Escherichia coli is the agent responsible for a range of clinical diseases. With emerging antimicrobial
resistance, other treatment options including solar/photo-therapy are becoming increasingly common. Visible Range
Radiation Therapy/Colour Therapy is an emerging technique in the field of energy/vibrational medicine that uses
visible spectrum of Electromagnetic Radiations to cure different diseases. In this study, our goal was to understand the
effect of Visible Range Electromagnetic Radiations on E. coli (in vitro) and therefore find out the most appropriate
visible range radiation for the treatment of diseases caused by E. coli.
Materials and Methods: A total of 6 non-repetitive E. coli isolates were obtained from urine samples obtained from
hospitalized patients with UTI. Single colony of E. coli was inoculated in 3 ml of Lysogeny Broth (LB) and 40 μl of
this E. coli suspension was poured into each of the plastic tubes which were then irradiated with six different
wavelengths in the visible region (Table. 1) after 18 hours with one acting as a control. The Optical Densities of these
irradiated samples were then measured. Furthermore, scanning electron microscopy (TEFCAN ZEGA3) was carried
out.
Results: The analysis of the microscopic and SEM images of irradiated E. coli samples with six different visible range
radiations is representative of The fact that E. coli responded differently to every applied radiation in the visible region
and the most profound inhibitory effects were that of 538nm Visible Range Radiation (Green) which proved to be
bactericidal and 590nm Visible Range Radiation (yellow) which was bacteriostatic. The enhanced growth of E. coli
with varying degrees was clearly observed in 610nm (orange), 644nm (red), 464nm (Purple) and 453nm (blue).
Conclusion: It can be concluded that 538nm (Green) and 590nm (Yellow) can effectively be used for treating E. coli
borne diseases
ESTABLISHMENT AND EVALUATION OF ORTHOTOPIC HEPATOCELLULAR CARCINOMA AND DRUG-INDUCED HEPATOCELLULAR CARCINOMA IN MICE WITH SPLEEN-DEFICIENCY SYNDROME IN TRADITIONAL CHINESE MEDICINE
Get PDFBackground: Spleen-deficiency syndrome (SDS) in Traditional Chinese Medicine (TCM) played pivotal roles on the
development of hepatocellular carcinoma (HCC). This study was performed to establish and evaluate HCC model in
mice with SDS in TCM.
Material and Methods: A total of 90 C57BL/6 mice were randomized in six groups (n=15 for each group): A, Control
group; B, SDS group; C, orthotopic HCC (OHCC) group; D, OHCC based on SDS (SDS-OHCC) group; E, Druginduced
HCC (DHCC) group; F, DHCC based on SDS (SDS-DHCC) group. The SDS model were established by
subcutaneous injection of reserpine, followed by the OHCC or DHCC model establishment. The SDS scores, tumor
formation rate and survival time were recorded and calculated, as well as the histochemical stain was performed.
Results: The SDS scores of mice in Group B, D, F were 17.57±4.86 (