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No full textAuthors file for The Database of African American and Predominantly White American Literature Anthologies (DALA
Cytosolic Ribosomal Protein Haploinsufficiency affects Mitochondrial Morphology and Respiration
No full textThe interplay between ribosomal protein composition and mitochondrial function is essential for sustaining energy homeostasis. Precise stoichiometric production of ribosomal proteins is crucial to maximize protein synthesis efficiency while reducing the energy costs to the cell. However, the impact of this balance on mitochondrial ATP generation, morphology and function remains unclear. Particularly, the loss of a single copy ribosomal protein gene is observed in Mendelian disorders like Diamond Blackfan Anemia and is common in somatic tumors, yet the implications of this imbalance on mitochondrial function and energy dynamics are still unclear. In this study, we investigated the impact of haploinsufficiency for four ribosomal protein genes implicated in ribosomopathy disorders ( rps-10, rpl-5, rpl-33, rps-23 ) in Caenorhabditis elegans and corresponding reductions in human lymphoblast cells. Our findings uncover significant, albeit variably penetrant, mitochondrial morphological differences across these mutants, alongside an upregulation of glutathione transferases, and SKN-1 dependent increase in oxidative stress resistance, indicative of increased ROS production. Specifically, loss of a single copy of rps-10 in C. elegans led to decreased mitochondrial activity, characterized by lower energy levels and reduced oxygen consumption. A similar reduction in mitochondrial activity and energy levels was observed in human leukemia cells with a 50% reduction in RPS10 transcript levels. Importantly, we also observed alterations in the translation efficiency of nuclear and mitochondrial electron transport chain components in response to reductions in ribosomal protein genes' expression in both C. elegans and human cells. This suggests a conserved mechanism whereby the synthesis of components vital for mitochondrial function are adjusted in the face of compromised ribosomal machinery. Finally, mitochondrial membrane and cytosolic ribosomal components exhibited significant covariation at the RNA and translation efficiency level in lymphoblastoid cells across a diverse group of individuals, emphasizing the interplay between the protein synthesis machinery and mitochondrial energy production. By uncovering the impact of ribosomal protein haploinsufficiency on the translation efficiency of electron transport chain components, mitochondrial physiology, and the adaptive stress responses, we provide evidence for an evolutionarily conserved strategy to safeguard cellular functionality under genetic stress
Geologic Map of the Bee Cave Quadrangle, Travis County, Texas
No full textProvides a summary report, geologic map database, Open-File Geologic map and supplemental data for the Bee Cave Quadrangle, Travis County, Texas
1900 Census Data Map Shapefile
No full textThis is the shapefile of the mapped 1900 census data for Austin, Texas
Experimental measurement data to perform forward/backward decomposition of dispersive wave propagation
No full textRepository for code and data accompanying the paper "Forward/backward
decomposition for dispersive wave propagation measurements" by Corbin and
Tarazaga.
The repository contains a readmefile, data and all needed codes to recreate the results in the publications. Further details about the experimental setup can be found in : Albakri, M.I.; Malladi, V.S.; Tarazaga, P.A. Low-frequency acoustoelastic-based stress state characterization: Theory and379
experimental validation. Mechanical Systems and Signal Processing 2018, 112, 417–429. https://doi.org/10.1016/j.ymssp.2018.04.01
Leveraging eHealth to manage adolescent obesity
No full textThis is the data used for the analysis of using eHealth to manage adolescent obesit
Replication Data for: A Hybrid Transistor with Transcriptionally Controlled Computation and Plasticity
No full textOrganic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, and neuromorphic computing. Despite their potential, developing programmable and modular methods for living systems to interface with OECTs has proven challenging. Here we describe hybrid OECTs containing the model electroactive bacterium Shewanella oneidensis that enable the transduction of biological computations to electrical responses. Specifically, we fabricated planar p-type OECTs and demonstrated that channel de-doping is driven by extracellular electron transfer (EET) from S. oneidensis. Leveraging this mechanistic understanding and our ability to control EET flux via transcriptional regulation, we used plasmid-based Boolean logic gates to translate biological computation into current changes within the OECT. Finally, we demonstrated EET-driven changes to OECT synaptic plasticity. This work enables fundamental EET studies and OECT-based biosensing and biocomputing systems with genetically controllable and modular design elements