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    2875 research outputs found

    Replication Data for: In Situ Optical Quantification of Extracellular Electron Transfer from Shewanella oneidensis using Plasmonic Metal Oxide Nanocrystals

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    Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on a variety of inorganic substrates, including metal oxides. For this reason, engineering EET processes has garnered significant interest for applications ranging from bioelectronics to materials synthesis. These applications require a strong understanding of electron flux from EET-relevant microbes. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes, which require long incubation times, electrode colonization, and convolute contributions to EET from planktonic cells. To address this, we developed a platform for quantifying time-resolved EET flux from cell suspensions using aqueous dispersions of plasmonic tin-doped indium oxide nanocrystals. Tracking the change in optical extinction during electron transfer and fitting the optical response to a free electron model enabled quantification of current generation and electron transfer rate constants from planktonic Shewanella oneidensis cultures. Using this method, we differentiated between starved and actively respiring S. oneidensis, and between cells of varying genotype using an EET knockout strain. In addition, we quantified current production ranging from 0.12 – 0.68 fA • cell-1 from S. oneidensis cells engineered to differentially express a key EET gene using an inducible genetic circuit. Overall, our results validate the utility of colloidally stable plasmonic metal oxide nanocrystals as quantitative biosensors in native biological environments and contribute to a fundamental understanding of planktonic S. oneidensis electrophysiology using simple in situ spectroscopy

    Composition of the Sub-Cratonic Mantle of the Guiana Shield Inferred from Diamond-Hosted Inclusions - Cathodoluminescence imagery, FTIR, and Raman Spectroscopic data

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    Cathodoluminescence imagery, FTIR, and Raman Spectroscopic dat

    TELEMAC-2D Raw Data Inputs

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    This dataset shares the raw input data used for the TELEMAC-2D model to simulate the San Antonio Bay water circulation

    Silk Stocking District

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    High resolution photogrammetric RAW JPGs of the Silk Stocking District located in Galveston, TX. Aerial imagery captured at 45 m altitude (GSD 1.24 cm) using DJI-RTK-UAS. Used to create 3D models with +/- 2 cm horizontal and +/- 5 cm vertical accuracies to ultimately derive first floor elevations (FFEs). GCPs provided in excel file

    Experiment 2

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    Data for experiment

    Ch. 1 - Maximum Economically Recoverable Storage (MERS)

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    Model code associated with the first chapter of Justin C. Thompson's Ph.D. dissertation regarding Maximum Economically Recoverable Storage (MERS). Originally authored in MATLAB. Two versions are provided for modeling either a confined aquifer or an unconfined aquifer

    Infographic use in cities

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    Coded data from visual content analysis of infographics found on municipal websites

    Data for Figure 1 in "The Devil's in the Details - or is He?" Perspectives on Terrorism 15, no. 2 (April 2021)

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    Data for Figure 1 in Benjamin V. Allison, "The Devil's in the Details - or is He? The Ethics and Politics of Terrorism Data," Perspectives on Terrorism 15, no. 2 (April 2021). This data includes individual cases that were miscoded in the dataset for the Center for Strategic and International Studies (CSIS) brief, "The Escalating Terrorism Problem in the United States" (June 2020). It provides details on the date and location of incidents, the original (i.e. CSIS) ideology attributed to incidents, the ideology as it should have been coded, an explanation as to why the original coding was wrong, and sources

    PST4PPD Home Visiting Postpartum Depression Intervention For Latina Mothers (Rebozos Main Outcome)

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    This is the data for the PST4PPD Home Visiting Postpartum Depression Intervention project. The intervention, PST4PPD (Problem-Solving Tools for Postpartum Depression), was delivered by 15 bilingual CHWs who were employed at five faith-based community organizations in four cities (Waukegan, IL; Phoenix, AZ; Houston, TX; Homestead, FL). The 74 (77%) participants who completed the intervention were included in the dataset

    Campeche Cove

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    High resolution photogrammetric RAW JPGs of the residential community Campeche Cove located in Galveston, TX. Aerial imagery captured at 100 m altitude (GSD 2.74 cm)using DJI-RTK-UAS. Used to create 3D models with +/- 2 cm horizontal and +/- 5 cm vertical accuracies to ultimately derive first floor elevations (FFEs). GCPs provided in excel file

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