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Gene delivery using chemical methods
No full textIn lieu of an abstract, here is the chapter\u27s 1st paragraph:
The bottleneck of gene therapy is the development of a method by which a therapeutic gene can be delivered to a cell site where gene expression can be accomplished. The process of introducing a gene into cells for the purpose of gene expression is called transfection. A number of methods have been developed for transfecting eukaryotic cells. These can be classified as either viral or nonviral. The ideal method for gene delivery should fulfill three basic requirements: 1. Protect against enzymatic degradation in the intracellular environment. 2. Transport the transgene across the plasma membrane and into the nucleus of the target cell. 3. Be devoid of any immunogenic response
Endangered Languages & Cultural Identity
No full textEye project is about finding a country or a people from a specific country that speaks a dying language and their culture is also becoming rare
Formulation and Stability of Doxorubicin-Albumin Gel: Implication for Cancer Therapy
No full textAlbumin hydrogels have emerged as promising nanoparticle-based systems for the delivery of anticancer drugs. Gel formation is achieved by modulating factors such as pH, temperature, ionic strength, and albumin concentration, which influence the ionic crosslinking behavior of albumin. Our previous work demonstrated that divalent cations significantly enhance the formation of clear, transparent albumin gels at lower temperatures compared to monovalent cations. The sol–gel transition of thermally denatured albumin is temperature-dependent, shifting from a soluble form to a structured gel network.
In this study, injectable doxorubicin (DOX)-loaded albumin hydrogels were formulated using monovalent cations (NaCl) to investigate controlled drug release and stability over 37 days. A robust, stability-indicating HPLC method was developed and validated for interday and intraday specificity to quantify doxorubicin within the gel matrix. Using this method, we successfully established the 1-month stability profile of DOX-albumin hydrogels
Alternative School District Lines in Monroe County NY
No full textPossible alternative school district lines in Monroe County to combat the historic policies of redlining in Rochester
Generating an in-vitro Model of MPS-IIIA “Sanfilippo Syndrome” via CRISPR/CAS9 and Exploring Potential Therapeutics in a PUI Setting
No full textAutosomal recessive inheritance of a defective SGSH gene coding for the enzyme sulfamidase is responsible for mucopolysaccharidosis (MPS) type IIIA, characterized by the effects of heparan sulfate accumulation in lysosomes. The syndrome presents with coarse facial features, central nervous system dysfunction, behavioral difficulties, intellectual disability, sleep disturbance, hyperactivity, loss of communication skills, and loss of mobility. Failure to develop cost effective research methods and long-term treatment options leaves the average age expectancy at just 10-20 years of age. Gene editing techniques including ZFNs, TALENs, and CRISPR/Cas9 have been explored as both models and potential therapeutic interventions for mucopolysaccharidosis types I, II and III. However, an in-vitro model of MPS IIIA, the most severe type of MPS, has yet to be developed. Therefore, we present a model test an in-vitro human cell line model of MPS IIA using a K562 lymphoblast cell line, CRISPR/Cas9 gene editing, and a heparan sulfate enzyme-linked immunosorbent assay (ELISA). We hypothesized that disruption of SGSH would reduce or eliminate function of the sulfamidase enzyme, resulting in toxic heparan sulfate accumulation in the cell that could be quantified by an ELISA. Furthermore, we hypothesized Fluoxetine, an experimental drug treatment, would reduce heparan sulfate levels in the cell based on previous evidence in an MPS-IIIA murine model. Our work demonstrated the K562 lymphoblast cell line is a cost-effective and viable cell type for quantifying heparan sulfate levels. Preliminary drug treatments also suggest Fluoxetine may be capable of reducing cellular heparan sulfate levels in-vitro. Altogether, this work points to the potential of creating a sustainable cell model of MPS-IIIA, with the possibility of recapitulating specific patient mutations via the CRISPR/Cas9 editing system in the future
Social and economic disparities and equity
No full textIn todays world many people often struggle with receiving proper health car
Ethical Considerations of Do-Not-Resuscitate (DNR) Orders in Suicidal Patients: Navigating Autonomy and Medical Duty
No full textWeighing and analyzing the ethics surrounding DNR in suicidal patients; based on course content (Biomedical Ethics Seminar
Methodological Variations in Air Displacement Plethysmography Body Composition Estimates Using 2- and 3-Compartment Models
No full textOur project looks at the differences between measured and estimated body composition measurements using a variety of different body composition measuring devices. The primary audience would be a poster presentation at a conference
