E-Journal Center for Plant Conservation Botanic Gardens-LIPI (Indonesian Institute of Sciences / Lembaga Ilmu Pengetahuan Indonesia)
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INDUKSI POLIPLOIDI MENGGUNAKAN KOLKISIN SECARA IN VIVO PADA BIBIT ANGGREK BULAN (Phalaenopsis amabilis (L.) BLUME)
Polyploid induction on the seedlings of Phalaenopsis amabilis has been done using colchicine under in vivo condition. Polyploid were induced by dripping colchicine to the shoot tip of P. amabilis seedlings. The objective of this study was to obtain an effective concentration of colchicine to induce polyploidy in P. amabilis seedlings and to produce polyploid seedlings. Experiment was arranged in randomize completely block design with one factor, the colchicine concentration. Seedlings of P. amabilis were dripped with 0,01 ml of colchicine solutions (0, 1000, 2000, 3000, 4000, and 5000 mg L-1). Results of the experiment showed that increasing colchicine concentration from 1000 to 5000 mg L-1 did not give significant effect to the survival and the growth of the seedlings which were observed at 24 weeks after treatment (WAT). Polyploid seedlings of P. amabilis could be produced by dripping colchicine at the concentration of 1000, 3000, 4000, and 5000 mg L-1 but the most effective concentration was 5000 mg L-1. Polyploid seedlings of P. amabilis have larger size with the lower density of stomata compared with their diploid counterparts
Perkecambahan Biji Bunga Bangkai Raksasa Amorphophallus titanum (Becc.) Becc. ex Arcang dengan Inisiasi Spatha Mini
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PENGEMBANGAN KOLEKSI TUMBUHAN KEBUN RAYA DAERAH DALAM KERANGKA STRATEGI KONSERVASI TUMBUHAN DI INDONESIA
As a member of the International Association of Botanic Gardens (IABG), Indonesian Botanic Gardens (IBG) has responsibilites to conduct plant conservation efforts and research in Indonesia. In fact, four botanic gardens managed by the Indonesian Institute of Sciences were only able to conserve approximately 21.5% of the Indonesian threatened plants. Therefore, it has been important to develop local/regional botanic gardens (L/RBG) throughout Indonesia in order to conserve the plant diversity. This paper aims to review and highlight the L/RBG achievements in plants conservation and to establish management strategy of plants collection in each region, especially in achieving the Target 8 of the GSPC. At the end of 2012, IBG and L/ RBG has collected and managed 24% of the Indonesian threatened plants and succesfully cultivated 25% of the Indonesian threatened plants (including in the nursery collections). Some strategies developed for the L/RBG plants collection development include: strengthening collection management and data base; developing and enriching plants collection in each L/RBG with reference to the IUCN Red List and local flagship species; and setting the priority species for conservation policy and actions
HIGHLAND SPECIES AND TEMPERATURE REQUIREMENT FOR GERMINATION: A CASE FROM TWO ENDEMIC PAPUAN Pittosporum (PITTOSPORACEAE) SPECIES
Climate change, including warming and drying, is currently the biggest challenge for plant regeneration. We conducted two experiments on how temperature affected the germination of Pittosporum pullifolium and P. spicessens, both endemic to Central Papua highlands. P. pullifolium habitat temperature at night could reach 8°C whereas P. spicessens habitat temperature ranged from 19°C early in the morning up to 26°C at midday. The first experiment was to understand the effect of chilling on P. pullifolium germination initiation. Our study showed that P. pullifolium was dependent on cold stratification for its germination. Without cold stratification the germination was absent even though the temperature range of sowing environment is at ca. 13–26°C (Cibodas Botanic Gardens). With a cold stratification at 6–8°C (constant) for more than a month, germination of
P. pullifolium occurred, with better germination rate under a light. Subsequently we carried out extended cold stratification for a month and interestingly, the germination still occurred but now it is better under dark condition. For P. spicessens, the germination at its habitat temperature range (Wamena) and in the warmer environment (Bogor Botanic Gardens), both occurred at more than two weeks after sowing
A NEW RECORD OF THE RHEOPHYTIC FERN Osmunda angustifolia (OSMUNDACEAE) FROM SUMATRA INCLUDING A NEW CYTOLOGICAL RECORD
Osmunda angustifolia Ching ex Ching et Wang (Osmundaceae) was, formerly, known to be distributed in China (Guandong), Hainan, Hongkong, and Taiwan. Now, O. angustifolia is reported as a newly recorded species in Sumatra. A complete morphological description with photographs is presented. It is defined as a rare species in Indonesia. A cytological record is also reported for the first time for the Sumatran species. The somatic chromosome number of root tip cells of O. angustifolia is 2n = 44 (diploid)
HABITAT ALAMI TUMBUHAN PAKU KIDANG (Dicksonia blumei (Kunze) Moore) DI KAWASAN HUTAN BUKIT TAPAK PULAU BALI
Dicksonia blumei (Paku Kidang) is one of the Indonesian priority species of conservation. A natural habitat study was carried out by purposive sampling method in Tapak hill, Bedugul, Bali. Three plots of 20 x 20 m were laid for trees and 2 x 2 m for understorey plants. Results showed that Dicksonia blumei (Paku Kidang) in Tapak Hill grows on environments with physical condition as follow: altitude 1,754–1,794 m asl, land slope 7–10%, soil pH 6.4–7, soil moisture 50–75%, air temperature 20.5–22.3 °C, relative humidity 83.2–87.5% and light intensity 618–10,003 Lux. Characters of Biotic environment is explained based on Important Value Index (IVI). Five trees with highest IVI were Cyathea latebrosa (IVI 98.7), Saurauia bracteosa (IVI 51.9), Astronia spectabilis (IVI 42.7), Dicksonia blumei (IVI 39.6), and Homalanthus giganteus (IVI 35.3). The same figure for understorey were Pilea sp. (IVI 61.9), Selaginella sp. (IVI 40,6), Athyrium asperum (IVI 27.5), Pteris tripartita (IVI 18.3) and Rubus sp. IVI (15.0). In Tapak hill, the occurrence of D. blumei along with Cyathea latebrosa seems to be associated with cyathea’s trunk as a substrate for the spores of D. blumei to germinate as well as the next growth stages. At the phase gametophyte and young sporophyte, D. blumei act as an epiphyte on Cyathea’s trunk at a height of 0,5– 1,5 m from the ground. Dicksonia’s root reaches the soil, it will grow terrestrially (hemi–epiphyte)