Rubatosis Publications (E-Journals)
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Method development and method validation of guaifenesin and dextromethorphan by RP-HPLC
No full textA new, simple, precise, accurate and reproducible RP-HPLC method for Simultaneous estimation of bulk and pharmaceutical formulations. Separation of Guaifenesin and Dextromethorphan was successfully achieve THERMO, C18, 250X4.6mm, 5µm or equivalent in an isocratic mode utilizing 0.1M KH2PO4: Methanol (60:40) at a flow rate of 1.0ml/min and eluate was monitored at 280nm, with a retention time of 3.259 and 4.164 minutes for Guaifenesin and Dextromethorphan respectively. The method was validated and there response was found to be linear in the drug concentration range of 50µg/ml to150 µg/ml for Guaifenesin and 50µg/ml to150 µg/ml for Dextromethorphan. The values of the correlation coefficient were found to 0.999 for Guaifenesin and 1for Dextromethorphan. respectively. The LOD and LOQ for Guaifenesin were found to be 0.597 and 1.991 respectively. The LOD and LOQ for Dextromethorphan were found to be 0.1072 and 0.3572 respectively. This method was found to be good percentage recovery for were found to be 99 and 100 respectively indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample so, the method specifically determines the analyte in the sample without interference from excipients of tablet dosage forms. The method was extensively validated according to ICH guidelines for Linearity, Accuracy, Precision, Specificity and Robustness
Current factors affecting the menstrual cycle
No full textA regular menstrual cycle is an important indicator of a healthy reproductive system. Menstrual problems affect not only women but also families, social & national economy as well. The menstrual cycle is divided into two 14-day phases. In normal healthy women, menarche occurs between the ages 10 and 16 years, with an average rhythm of 28 days, inclusive of 4–6 days of bleeding, with some common minor variations like Amenorrhoea, Oligomenorrhoea, Polymenorrhoea, Menorrhagia, Hypomenorrhoea, etc., these menstrual disorders frequently affect the quality of life of young adults women, some of the lifestyle factors like obesity, intake of junk food, stress, lack of physical activity, skipping breakfast are associated with irregular menstruation. The purpose of this study is to identify menstrual irregularities and assess the impact of lifestyle factors and on the menstrual patterns in reproductive-age females