2923 research outputs found
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Ladies in a Buckboard Wagon
No full textBlack and white photograph, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1021/thumbnail.jp
J.A. Adams Home- Corner E.S. Line and S. Vienna
No full textBlack and white photograph, Ruston Louisiana, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1028/thumbnail.jp
Interior of Mays and Holland Store- 102 W. Park
No full textBlack and white photograph, Ruston Louisiana, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1035/thumbnail.jp
Exterior of Minnie J. Tims Fashionable Milliner
No full textBlack and white photograph of Ruston, Louisiana, ca, 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1036/thumbnail.jp
Cargill Building- Cargill\u27s Store-Corner of W. Miss and North Trenton-Lincoln Parish Bank-Later F. National
No full textBlack and white photograph of Ruston, Louisiana, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1037/thumbnail.jp
Ruston Hardware-Interior View of Salesroom
No full textBlack and white photograph, Ruston, Louisiana, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1053/thumbnail.jp
Gathering for a Red Cross Drive WWI- Building was the Culture Club
No full textBlack and white photograph, Ruston, Louisiana, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1061/thumbnail.jp
Hot Air Balloon Ascension in Railroad Park- Steeple in background is original Trinity Methodist Church-301 N. Trenton
No full textBlack and white photograph of Ruston, Louisiana, ca. 1900.https://digitalcommons.latech.edu/ruston-photograph-collection/1059/thumbnail.jp
Using CRISPR-Cas9 as a Restriction Enzyme
Get PDFRestriction digests are a commonly utilized process for cleaving DNA at specific, but relatively common sites. Restriction enzymes have widespread use in DNA manipulation. CRISPR/Cas9 is a recently identified endonuclease which utilizes a customizable guide sequence to recognize and cut specific ~20 bp sites located in a DNA sequence. This preliminary research aimed to exploit the potential benefit of DNA restriction using the CRISPR/Cas9 procedure through alterations of different components involved in that system. We sought to refine existing CRISPR/Cas9 protocols and make a budget friendly, user-selectable CRISPR/Cas9 restriction digest protocol. The motivation for this research was to simplify and adapt known CRISPR protocols in hopes of using CRISPR as a targeted restriction enzyme. This project yielded negative results, however, important insights into the dilution of target sequences was achieved
A Multi-State Evaluation of the Knowledge of the Background and Dangers of Popular Fad Diets Among Cooperative Extension Agents in Family And Consumer Sciences
Get PDFCooperative extension provides communities with research-based information in several areas including family and consumer sciences (FCS) (Franz & Townson, 2008). Because FCS agents teach communities about dietary guidelines, it is important that they are aware of the latest nutrition research. This study assessed the knowledge of popular fad diets and their potential adverse effects among FCS cooperative extension agents, and its relationship among the variables of location, professional certifications held, years of extension work experience, membership in professional associations, and education level. FCS extension agents in Louisiana, Texas, Arkansas, and Mississippi were emailed an online survey, which collected demographics and assessed their knowledge about three fad diet categories (low-carbohydrate, intermittent fasting, detoxes and cleanses) and potential adverse effects. Data collection lasted for four weeks. One hundred and thirtyeight agents were analyzed. Frequency testing was used for descriptive statistics of demographic data. Respondents’ knowledge about fad diets and adverse effects was assessed with test scores from knowledge items embedded in the survey. The average total knowledge score among respondents was 70%. Univariate ANOVA was used to compare scores with education level, years of experience, membership in professional associations, certifications held, and the state in which participants were employed. A pvalue of ≤ 0.05 indicated significance. RDN, DTR, and CHES certifications had a significant association (p = 0.03) with knowledge adverse effects of fad diets. The results suggest agents who hold these credentials may have more knowledge about adverse effects of fad diets. Further research is needed to validate this association