Marine Research in Indonesia (MRI - E-Journal)
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    ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA)

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    Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum

    UTILIZATION OF COASTAL ZONE IN INDONESIA. PRESENT STATUS OF OYSTER, SEAWEED AND COCKLE CULTURE

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    Maricullure in coastal zone, such as the bays, inlets, or lagoons in coral reef is becoming more important in the world fishery activities. In Indonesia, some culture experiments are being conducted, such as for oyster (Crassostrea tuculata BORN) in Banten Bay, and seaweed (Eucheuma spinosum) in Samaringa Islands (Central Sulawesi) and Talang islands (Riau Archipelago), while culture of cockle (Anadara granose LIN.) based on a simple method have been conducted by local fishermen from Ketapang (West Jawa) since several years ago. Some preliminary results of culture experiments are given

    ON THE FACTORS AFFECTING THE PRODUCTIVITY OF THE SOUTHERN MAKASSAR STRAIT

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    An investigation of the upwelling in the Southern Makassar Strait was conducted by LON (Lembaga Oseanologi Nasional) as part of its research program in ecology and oceanography of the Indonesian waters. It consists of observation on one monitoring station and two oceanographical cruises in the region. The result of the monitoring station indicates clearly the occurrence of upwelling and its effect on the hydrology of the region. During the southeast monsoon the upwelled water causes a decrease in temperature and dissolved oxygen and an increase in salinity, density and nitrate-nitrogen, particularly seen at the lower boundary of the homogeneous layer. During the northwest monsoon the upwelled water is replaced by the Jawa Sea water with high temperature, low oxygen, low salinity, low density, very high phosphate, and high silicate. The observation on the oceanographical cruises reveals the precence of two other types of surface waters in the region. They are the East Kalimantan Coastal Water and the South Sulawesi Coastal Water, each is formed by mixing process of sea water and land drainage along the coast of the two islands respectively. Originally they are characterized by low salinity, low oxygen, high temperature, high phosphate, low nitrate and high silicate. Their salinity and dissolved oxygen increase due to mixing with the upwelled water and the high activity of phytoplankton respectively

    THE RELATIONSHIP BETWEEN CONDITION FACTOR AND MEAT YIELD OF WILD OYSTER CRASSOSTREA CUCULLATA BORN

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    From April 1982 to October 1982, 393 specimens of oyster were collected near mangrove roots from Piru, Waisala, Wailale, Pelita Jaya (Seram Island) Ihamahu (Saparua Island), and Manipa Island for sondition factor analysis. The regression of meat yield (Y) on whole meat weight (X1) and condition factor (X2) could be expressed by the following equation: Y = 0.062 X1 + 0.127 X2 — 10.975. The analysis of correlation indicated that the meat yield of oyster depends on the condition factor. Hydrologieal parameters which affected the condition factor are described

    ULTRASTRUCTURE OF THE ENDOSYMBIOTIC DINOFLAGELLATE Symbiodinium microadriaticum LIVING IN THE SEA ANEMONE Anemonia viridis

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    The zooxanthella, Symbiodinum microadriaticum, an endosymbiotic dinoflagellate shows variation in its ultrastructure within its population in the sea anemone, Anemonia viridis. Such variation included the number of thylakoid, the structure of inclusions and the structure of amphiesma. The string-like structure was also found in the nucleoplasm. Some zooxanthellae have a branching or double pyrenoid with two or three stalks. Under certain condition, which are not clearly understood, two or three zooxanthellae are enclosed within one very thick membrane

    SEDIMENT TRANSPORT IN THE BASIN OF PULAU BAAI PORT, BENGKULU

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    The 2D horizontal model has been simulated to investigate a sedimentation and erosion pattern in the basin of Pulau Baai port — Bengkulu Sumatera. The model was verified with observed data from the Indonesian Research Center for Marine Geology (Arifin et al., 2001). The simulation results of the hydrodynamic model shows a good agreement with the observed data. In -regard to the elevation of sea level, the difference between simulation and the observed data is about 4.6 cm, giving averaged error of about 2.93 %, and the difference in velocity is 6.8 cm/s or in the order of error of about 20.1 %. The results of the model on sediment transport qualitatively show comparable values to the observed ones, although in some parts of the area, the model is still to be adjusted. Simulation results show that the strong current occurs in the harbor channel during the time of spring flood tide with a speed of about 95.4 cm/s, while the ebb current is 54.1 cm/s. The results of the model on sediment transport show that the erosion area occurs in the northeastern part of the basin and sedimentation takes place in the southwestern to southeastern of the channel. The channel port is generally eroded, and sedimentation occurs in the mouth and the western part of offshoreward of the channel, and erosion is identified in the eastern part of the offshoreward of the channel

    THE FISHES OF SEAGRASS COMMUNITY ON THE WEST SIDE OF BURUNG ISLAND (PARI ISLANDS, SERIBU ISLANDS) AND THEIR VARIATIONS IN ABUNDANCE

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    Monthly collections of fishes in a seagrass community on the west of Burung Island, Pari Islands, were made from May 1975 to February 1976. Seventy-eight species of fishes were collected. Number of specimens, size range, and modal size are given for each month for each species. Number of species and specimens were found highest in July. Relative and total abundances of the more numerous species are summarized. Apogon margaritophorus was the predominant species in the fish community of the area. Notes on the spawning of Siganus canaliculatus are presented, since this species attracts the attention of many mariculturists. The separation of these fishes into four groups is also discussed

    CHEMICAL COMPOSITIONS OF TWO BROWN SEAWEED SPECIES FROM KARIMUN JAWA, INDONESIA

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    Seaweeds are potentials to be developed as alternative source in foods and pharmaceuticals. In this study, chemical compositions of two brown seaweeds species from Karimun Java Indonesia Padina australis and Turbinaria conoides were investigated. Proximate results showed that both seaweeds contain high amount of carbohydrates and ashes. Mineral contents of P. australis and T. conoides follow the orders of Na>Mg>Fe>K>Ca>Zn>Cu and Na>Mg>K>Fe>Zn>Ca>Cu, respectively. Almost all essential amino acids (including histidine, isoleucine, leucine, phenylalanine, threonine, valine and lysine) were present in both seaweeds. Amino acid profiles showed that both seaweed contain polyunsaturated fatty acids (PUFA) with T. conoides contain higher amount of EPA (8.58 ± 0.22 g/100 g of total fatty acids) and DHA (6.05 ± 0.21 g/100 g of total fatty acids). The findings of this study have provided evidence that brown seaweeds were nutritious and potential to be utilized for producing functional ingredients in food. Further, P. australis and T. conoides can be used as candidate to be used in food industries to increase shelf-life of food items for human consumption, and use to deter deleterious free radical-induced life-threatening diseases

    REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra)

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    The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development

    THE FIRST RECORD OF Holothuria (Semperothuria) Roseomaculata KERR, 2013 (Echinodermata: Holothuroidea) IN INDONESIA

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    This paper documents the first record of Holothuria (Semperothuria) roseomaculata Kerr, 2013 (Echinodermata: Holothuroidea) from Indonesia. A freshly salted specimen was collected from fishers in Sumbawa, West Nusa Tenggara, Indonesia. This species was previously recorded from Yap and Chuuk States in the Federated States of Micronesia, Papua New Guinea, Palau, Okinawa-Japan, Sabah, Borneo-Malaysia, Solomon Islands, Vanuatu and New Caledonia. Reference material has been deposited in the Reference Collection of the Research Center for Oceanography, Indonesian Institute of Sciences in Jakarta, Indonesia.

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