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Replication Data for: Slapin et al. (forthcoming) "Ideology, Grandstanding, and Strategic Party Disloyalty in British Parliament" American Political Science Review
No full textThese files contain all the materials necessary for complete replication of the analyses found in Slapin et al. (forthcoming) "Ideology, Grandstanding, and Strategic Party Disloyalty in British Parliament" American Political Science Review
Extracellular loops 2 and 3 of the calcitonin G protein-coupled receptor selectively modify agonist binding and efficacy
No full textSupporting information for: Del Maso et al., Extracellular loops 2 and 3 of the calcitonin G protein-coupled receptor selectively modify agonist binding and efficac
CLR / RAMP / CGRP / AM Molecular dynamics simulations (500 ns)
No full textDataset for
Woolley et al., Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs. Biochemical Pharmacology, 2017
Article abstract: Calcitonin gene-related peptide (CGRP) or adrenomedullin (AM) receptors are heteromers of the calcitonin receptor-like receptor (CLR), a class B G protein-coupled receptor, and one of three receptor activity-modifying proteins (RAMPs). How CGRP and AM activate CLR and how this process is modulated by RAMPs is unclear. We have defined how CGRP and AM induce Gs-coupling in CLR-RAMP heteromers by measuring the effect of targeted mutagenesis in the CLR transmembrane domain on cAMP production, modeling the active state conformations of CGRP and AM receptors in complex with the Gs C-terminus and conducting molecular dynamics simulations in an explicitly hydrated lipidic bilayer. The largest effects on receptor signaling were seen with H295A5.40b, I298A5.43b, L302A5.47b, N305A5.50b, L345A6.49b and E348A6.52b, F349A6.53b and H374A7.47b (class B numbering in superscript). Many of these residues are likely to form part of a group in close proximity to the peptide binding site and link to a network of hydrophilic and hydrophobic residues, which undergo rearrangements to facilitate Gs binding. Residues closer to the extracellular loops displayed more pronounced RAMP or ligand-dependent effects. Mutation of H374A to alanine increased AM potency 100-fold in the CGRP receptor. The molecular dynamics simulation showed that TM5 and TM6 pivoted around TM3. The data suggest that hydrophobic interactions are more important for CLR activation than other class B GPCRs, providing new insights into the mechanisms of activation of this class of receptor. Furthermore the data may aid in the understanding of how RAMPs modulate the signaling of other class B GPCRs
Equilibrated structures for the MD simulations
Full text linkSupporting data for Equilibrated structures for the MD simulations. For further detail see 000README.txt and par_all36_prot_lip.prm
For published article see, Bower, RL and Yule, L and Rees, TA and Deganutti, G and Hendrikse, ER and Harris, PWR and Kowalczyk, R and Ridgway, Z and Wong, AG and Swierkula, K and Raleigh, DP and Pioszak, AA and Brimble, MA and Reynolds, CA and Walker, CS and Hay, DL (2018) 'Molecular signature for receptor engagement in the metabolic peptide hormone amylin.' ACS Pharmacology & Translational Science. ISSN 2575-9108 https://pubs.acs.org/doi/10.1021/acsptsci.8b00002 and http://repository.essex.ac.uk/21918
CLR MD coordinates for Woolley et al., 2017, Mol. Endocrinol.
No full textMolecular dynamics coordinates linked to Woolley et al., (2017) Understanding the molecular functions of the second extracellular loop (ECL2) of the Calcitonin Gene-Related Peptide (CGRP) receptor using a comprehensive mutagenesis approach, Mol. Endocrinol
The Collaborative Puzzle Game corpus
No full textThe Collaborative Puzzle Game corpus is based on an experiment conducted in 2016 at the Department of Psychology of the University of Essex. Interactions between 30 pairs of participants (all native English speakers) were included in the corpus. The corpus includes 83,173 words and 10,385 speech turns
The Collaborative Puzzle Game corpus
No full textThe Collaborative Puzzle Game corpus is based on an experiment conducted in 2016 at the Department of Psychology of the University of Essex. Interactions between 30 pairs of participants (all native English speakers) were included in the corpus. The corpus includes 83,173 words and 10,385 speech turns. See also http://repository.essex.ac.uk/22298
Ostrea edulis Essex Estuaries (public ground) location and size class distribution
No full textLocation and distribution of Ostrea edulis on the publicly accessible ground Blackwater Estuary and surrounding coastal zon
GLP-1R model structures for Wootten et al., Molecular Pharmacology, submitted 2015
No full textReadme File
model structure of the full GLP-1R, generated using Modeller
model structure of the apo inactive GLP-1R, generated using PLOP
movie showing the trajectory of the apo inactive GLP-1
Going the extra green mile: When others' actions fall short of their responsibility
No full textThe present research examined whether the environmental responsibility and actions attributed to large scale organizations, such as the government, can influence people's environmental efforts. In particular, we examined whether people increase or decrease their willingness to enact energy conservation behaviors (ECB) when there is a shortfall between others' actions and their responsibility.
In Studies 1 and 2 we utilized a correlational design to examine if willingness to enact ECB were associated with perceptions of others environmental responsibilities, actions, and the discrepancies between others responsibility and actions. In Study 3, we employed an experimental design and manipulated the extent to which the government's actions were seen as falling short of their responsibility to examine the effects on willingness to enact ECB