AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
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The Influence of Leaf Fertilizer Media on Multiplication of Barangan Banana (Musa acuminate L.) in vitro
Full text linkBarangan banana (Musa acuminata L.) is a plant that has the biggest contribution to national fruit production. The increasing demand for the banana requires the availability of seedlings, while conventional cultivation is unable to produce healthy, disease-free seedlings in a short time and large amount. In vitro culture is considered to be able to handle the constraints of the conventional seed supply. The purpose of this study was to determine the effect of foliar fertilizers on in vitro multiplication of barangan bananas. The method used was a single factor of Completely Randomized Design (CRD), the addition of leaf fertilizer consisting of five levels of media treatment, media MS (control), and 0.50 ml L-1 (p1); 0.75 ml L-1 (p2); 1.00 ml L-1 (p3); 1.25 ml L-1 (p4); 1.50 ml L-1 (p5) of leaf fertilizer media repeated five times. The data were analyzed with Analysis Variance then followed by Duncan Multiple Ranged Test of 5%. The results showed MS media still gave the best results compared to other leaf fertilizer media. Meanwhile, the leaf fertilizer media used in this study still gave good results on the initial time of buds appearance (p3 treatment), the number of shoots and the number of leaves in the p5 treatment, as well as the height of the plant in the p4 and p5 treatments. Therefore, the leaf fertilizer can be used to substitute MS mediaBarangan banana (Musa acuminata L.) is a plant that has the biggest contribution to national fruit production. The increasing demand for the banana requires the availability of seedlings, while conventional cultivation is unable to produce healthy, disease-free seedlings in a short time and large amount. In vitro culture is considered to be able to handle the constraints of the conventional seed supply. The purpose of this study was to determine the effect of foliar fertilizers on in vitro multiplication of barangan bananas. The method used was a single factor of Completely Randomized Design (CRD), the addition of leaf fertilizer consisting of five levels of media treatment, media MS (control), and 0.50 ml L-1 (p1); 0.75 ml L-1 (p2); 1.00 ml L-1 (p3); 1.25 ml L-1 (p4); 1.50 ml L-1 (p5) of leaf fertilizer media repeated five times. The data were analyzed with Analysis Variance then followed by Duncan Multiple Ranged Test of 5%. The results showed MS media still gave the best results compared to other leaf fertilizer media. Meanwhile, the leaf fertilizer media used in this study still gave good results on the initial time of buds appearance (p3 treatment), the number of shoots and the number of leaves in the p5 treatment, as well as the height of the plant in the p4 and p5 treatments. Therefore, the leaf fertilizer can be used to substitute MS medi
Isolasi dan Seleksi Cendawan Rhizosfer dan Endofit asal Tanaman Kelor sebagai Agens Penginduksi Perkecambahan pada Benih Padi: Isolasi dan Seleksi Cendawan Rhizosfer dan Endofit asal Tanaman Kelor sebagai Agens Penginduksi Perkecambahan pada Benih Padi
No full textRhizosphere and endophytic fungi are functional types of microbes capable of producing secondary metabolites that can affect plant growth directly or indirectly. This study aims to isolate and test the rhizosphere and endophytic fungi\u27 ability from Moringa (Moringa oleifera L.) against rice seeds\u27 viability and vigor. Fungus exploration was carried out on soil samples in the rhizosphere and stem and leaf tissue of healthy Moringa plants. Isolation of fungi from the rhizosphere was carried out using 10-2 and 10-3 dilution techniques, while the isolation of endophytic fungi was carried out on the leaf and stem tissue of Moringa, then cultured on potato dextrose agar (PDA) medium. The pathogenicity test of fungi and its effect on in-vitro rice seed germination using the blotter test method, namely growing 25 rice seeds on seven-day old fungal isolates. Nineteen fungal isolates were isolated and collected from the parts of the Moringa plant. Pathogenicity observations showed that there were five fungal isolates as potential pathogens, namely isolates RF2, RF5, RF6, RF8, and EDF6. A total of four fungal isolates tested consistently showed a positive effect on seed viability and vigor with a value of ≥90%, namely isolates RF4, EDF1, EDF2, and EDFbt3.Cendawan rhizosfer dan endofit merupakan jenis mikroba fungsional yang mampu memproduksi metabolit sekunder yang dapat memengaruhi pertumbuhan tanaman baik secara langsung atau tidak langsung. Penelitian ini bertujuan mengisolasi dan menguji kemampuan cendawan rhizosfer dan endofit asal tanaman kelor terhadap viabilitas dan vigor kecambah benih padi. Eksplorasi cendawan dilakukan terhadap sampel tanah di bagian rhizosfer serta jaringan batang dan daun tanaman kelor sehat. Isolasi cendawan dari tanah bagian rhizosfer dilakukan dengan teknik pengenceran 10-2 dan 10-3, sedangkan cendawan endofit dilakukan pada jaringan daun dan batang tanamn kelor, kemudian dibiakkan pada media medium potato dextrose agar (PDA). Uji patogenisitas cendawan dan pengaruhnya terhadap perkecambahan benih padi secara in-vitro dengan metode blotter test, yaitu dengan cara menumbuhkan benih padi sebanyak 25 butir pada isolat cendawan berumur 7 hari. Sebanyak sembilan belas isolat cendawan berhasil diisolasi dan dikoleksi dari berbagai bagian tanaman kelor. Pengamatan patogenisitas menunjukkan terdapat lima isolat cendawan yang berpotensi sebagai patogen, yaitu isolat RF2, RF5, RF6, RF8, dan EDF6. Sebanyak empat isolat cendawan yang telah diuji konsisten memberikan pengaruh positif terhadap viabilitas dan vigor benih benih dengan nilai ≥90% yaitu isolate RF4, EDF1, EDF2, dan EDFbt
Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine
No full textGenetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%)
Potensi Bakteri Asal Bambu dalam Memproduksi Asam Indol Asetat (IAA): Potensi Bakteri Asal Bambu dalam Memproduksi Asam Indol Asetat (IAA)
No full textBamboo are known having a high adaptive ability to tolerate environmental changes or stresses. Endogenous microorganisms in several parts of bamboo have been reported used as organic fertilizer and biocompost. However, bacterial potential as auxin (IAA) producer has not been widely report, especially for Torajas’ local bamboo. In this study, rhizosphere and endophytic bacteria were isolated from six different bamboo. Bamboo samples were obtained from the bamboo forest station area of North Toraja. Rhizosphere bacterial isolates were obtained from the area around the roots of bamboo plants, while endophytic bacterial isolates were obtained from roots and shoots bamboo tissue. Six rhizosphere isolates and 12 endophytic isolates were obtaind. All isolates were indentified for morphological, physiological, biochemical tests and IAA’s activities. There are 12 IAA-producing isolates, which where dominated by endophytic bacterial isolates. Based on 16S molecular identification, it was found that K12 isolates were similar to Bacillus cereus, with an IAA concentration value was 1.301 mg L-1. While K14 isolated has similiarities with Stenotrophomonas maltophilia with the abiliy to produce IAA was 2.737 mg L-1. The reconstruction of the phylogeny tree showed that K12 isolate had similiarity with Bacillus wedimannii, and K14 isolate was related to Stenotrophomonas sp.Bambu dikenal sebagai memiliki kemampuan adaptif yang tinggi dalam mentoleransi perubahan ataupun cekaman lingkungan. Mikroorganisme lokal pada beberapa bagian tanaman bambu telah banyak dilaporkan dapat dimanfaatkan sebagai pupuk organik dan biokompos, namun potensi bakteri asal tanaman bambu sebagai penghasil IAA belum banyak dilaporkan. Dalam penelitian ini dilakukan isolasi bakteri rizosfer dan endofit yang berasal dari enam jenis bambu berbeda. Sampel bambu diperoleh dari kawasan hutan bambu Stasiun Mengkendek Tana Toraja. Isolat bakteri rizosfer diperoleh dari daerah sekitar perakaran tanaman bambu, sedangkan isolat bakteri endofit diperoleh dari jaringan segar akar dan rebung bambu. Dalam penelitian ini diperoleh 6 isolat bakteri rizosfer dan 12 isolat bakteri endofit. Seluruh isolat diidentifikasi bentuk sel, tipe Gram, uji biokimia, serta diukur kemampuannya dalam memproduksi IAA. Dari 18 isolat, diperoleh 12 isolat penghasil IAA. Isolat bakteri endofit mendominasi perolehan isolat penghasil IAA. Dari hasil identifikasi 16S diketahui isolat K12 memiliki kemiripan dengan Bacillus cereus dan mampu menghasilkan IAA sebesar 1.301 mg L-1, sedangkan isolat K14 memiliki kemiripan dengan Stenotrophomonas maltophilia dengan kemampuan menghasilkan IAA sebesar 2.737 mg L-1. Hasil rekonstruksi pohon filogeni menunjukkan isolat K12 memiliki kemiripan pasangan basa dengan Bacillus weidmannii, dan isolat K14 memiliki kekerabatan dengan kelompok Stenotrophomonas sp
Respon Pertumbuhan dan Produksi Sorgum (Sorghum bicolor [L.] Moench.) dengan Pemberian Berbagai Dosis Amelioran di Lahan Pasca Tambang Timah: Pengaruh Dosis Pupuk Kotoran Ayam dan NPK terhadap Pertumbuhan dan Hasil Tanaman Sorgum di Lahan Pasca Tambang Timah
No full textPost tin mining land is one of marginal land because it has low nutrient availability. Efforts to utilize post tin mining land in agriculture for crop cultivation such as sorghum by application various doses of chiken manure an N,P,K. The purpose of this study was to determine the response of sorghum growth and production in post tin mining land by application various doses of chicken manure and N,P,K. This research used a factorial randomized block design with 6 treatment combinations and 4 groups. The first factor was the dose of chicken manure consisting of 100%, 50%, and 25% the reference dose. The second factor was the dose of fertilizer N, P, K which consists of 100% and 50% the reference dose. The results showed that chicken manure significantly affected leaves number parameters. Fertilizer N, P, K gave very significant effect on plant height, stem diameter, wet stover weight, root length, seed yield per plant and productivity. Fertilizers N, P, K significantly affect the total parameters of dissolved solids. The reduction of the dose of chicken manure from 100% to 50% showed no significant difference on leaves number. Reduction of N, P, K fertilizer doses showed significantly different results on all parameters. The A1K1 treatment (100% chicken manure + 100% N,P,K) is the best combination of treatment for the growth and production of sorghum in post tin mining land.Lahan pasca tambang timah termasuk dalam karakteristik lahan marjinal karena memiliki tingkat kandungan hara yang rendah. Upaya pemanfaatan lahan pasca tambang timah pada pertanian yaitu budidaya tanaman seperti sorgum dengan pemberian berbagai dosis pupuk kotoran ayam dan N,P,K. Tujuan dari penelitian ini adalah mengetahui respon pertumbuhan dan produksi sorgum di lahan pasca tambang timah dengan pemberian berbagai dosis pupuk kotoran ayam dan N,P,K. Penelitian ini menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Faktor pertama adalah dosis pupuk kotoran ayam yang terdiri dari 100 %, 50 % dan 25 % dosis acuan. Faktor kedua adalah dosis pupuk N,P,K yang terdiri dari 100 % dan 50 % dosis acuan. Hasil penelitian menunjukkan pupuk kotoran ayam berpengaruh nyata terhadap parameter jumlah daun. Pupuk N,P,K berpengaruh sangat nyata terhadap parameter tinggi tanaman, diameter batang, bobot berangkasan basah, panjang akar, hasil biji per tanaman dan produktivitas. Pupuk N,P,K berpengaruh nyata terhadap parameter total padatan terlarut. Pengurangan dosis pupuk kotoran ayam dari 100 % ke 50 % menunjukkan hasil berbeda tidak nyata terhadap jumlah daun. Pengurangan dosis pupuk N,P,K menunjukkan hasil berbeda nyata terhadap semua parameter. Perlakuan A1K1 (pupuk kotoran ayam 100 % + pupuk N,P,K 100 %) merupakan kombinasi perlakuan terbaik untuk pertumbuhan dan produksi sorgum di lahan pasca tambang timah
Keanekaragaman Lalat Buah (Diptera: Tephritidae) di Pertanaman Jeruk Manis di Kabupaten Bangka Tengah, Provinsi Kepulauan Bangka Belitung: Keanekaragaman Lalat Buah (Diptera: Tephritidae) di Pertanaman Jeruk Manis di Kabupaten Bangka Tengah, Provinsi Kepulauan Bangka Belitung
No full textCentral Bangka Regency is one of central orange production in Bangka Belitung Islands Province. One of the important pests of sweet orange plants is fruit flies (Diptera: Tephritidae). This research aimed to study the diversity of fruit flies (Diptera: Tephritidae) on sweet orange plantations in Central Bangka Regency, Bangka Belitung Islands Province. The fruit flies were collected using Lynfield traps which installed in three villages producing sweet oranges in Central Bangka Regency. The types of attractants used to trap male fruit flies are methyl eugenol and cue lure. The fruit flies collected during the study were 4795 individuals, 3 genera, and 14 species. Fruit flies collected from the methyl eugenol were different compared to those collected using lure attractant trap. Most species (13 species) of fruit fly were found in Terentang village, and the rest was same (9 species). Bactrocera carambolae and B. dorsalis were the dominant fruit fly species in the three research locations.Kabupaten Bangka Tengah merupakan salah satu kabupaten penghasil jeruk manis di Provinsi Kepulauan Bangka Belitung. Salah satu hama penting tanaman jeruk manis adalah lalat buah (Diptera: Tephritidae). Penelitian ini bertujuan untuk mempelajari keanekaragaman Lalat buah (Diptera: Tephritidae) di pertanaman jeruk manis di Kabupaten Bangka Tengah, Bangka Belitung. Lalat buah dikoleksi dengan perangkap Lynfield trap yang dipasang di tiga desa penghasil jeruk manis di Kabupaten Bangka Tengah. Jenis atraktan yang digunakan untuk memerangkap lalat buah jantan yaitu metil eugenol dan cue lure. Lalat buah yang terkoleksi selama penelitian sebanyak 4795 individu, 3 genus, dan 14 spesies. Lalat buah yang dikoleksi dari perangkap atraktan metil eugenol teridentifikasi berbeda dengan lalat buah yang terperangkap dengan atraktan cue lure. Spesies lalat buah paling banyak ditemukan di desa Terentang sebanyak 13 spesies, dan di desa lain masing-masing ditemukan 9 spesies lalat buah. Bactrocera carambolae dan B. dorsalis merupakan spesies lalat buah dominan di tiga lokasi penelitian
Variabilitas, Korelasi, dan Analisis Kelas Sepuluh Genotipe Nilam (Pogostemon cablin Benth.): Variabilitas, Korelasi, dan Analisis Kelas Sepuluh Genotipe Nilam (Pogostemon cablin Benth.)
No full textPatchouli is a plant that produces essential oils that cannot be substituted with other essential oils. The genetic diversity of patchouli is low, because in Indonesia there are only five superior clones, and this is caused a limitation for cultivation. Therefore it is necessary to produce new superior clones filled by national standards. This study tested nine patchouli superior genotypes resulting from mutations. The main objective of this research were to obtain the results stability, wide variability, to determine the correlation of each parameter and class closeness among ten patchouli genotypes. The study used nine plant clones of patchouli from the collection and one patchouli variety released in Indonesia as a comparison, namely Lhoksemauwe. The treatments were arranged in a completely randomized block design with three replications. Based on the results of the study, there was no significant difference in almost all observed parameters except for the stem diameter parameter. The genotype used has shown the stability of the results at the Rajabasa location in South Lampung. The variability in almost all parameters showed broad criteria, except for the parameters of cell turgidity and oil yield. Significant positive correlations occurred between several observed parameters. There are four classes formed and three of it have more than 90% closeness.Tanaman nilam merupakan tanaman penghasil minyak atsiri dengan nilai ekonomi tertinggi dibandingkan tanaman penghasil minyak atsiri lainnya. Keragaman genetik tanaman nilam tergolong rendah, karena di Indonesia baru terdapat lima klon unggul, dan keterbatasan ini menyebabkan pilihan varietas untuk budidaya juga terbatas. Oleh karena itu perlu dilakukan upaya untuk menghasilkan klon unggul baru yang memenuhi standar nasional. Penelitian ini menguji sembilan genotipe unggul nilam hasil mutasi pada dataran rendah. Tujuan utama penelitian untuk mendapatkan kestabilan hasil, variabilitas yang luas, mengetahui korelasi tiap parameter, dan kedekatan kelas sepuluh genotipe nilam. Penelitian menggunakan bahan tanam sembilan klon tanaman nilam hasil koleksi dan satu varietas nilam yang telah rilis di Indonesia sebagai pembanding, yaitu Lhoksemauwe. Perlakuan disusun dalam Rancangan Kelompok Teracak Sempurna dengan tiga ulangan. Berdasarkan hasil penelitian tidak terdapat perbedaan signifikan pada hampir seluruh parameter pengamatan kecuali pada parameter diameter batang. Genotipe yang digunakan sudah menunjukkan kestabilan hasil pada lokasi Rajabasa Lampung Selatan. Variabilitas pada hampir seluruh parameter menunjukkan kriteria luas kecuali pada parameter turgiditas sel dan rendemen minyak. Korelasi positif yang signifikan terjadi antara beberapa parameter pengamatan. Kelas yang terbentuk sebanyak empat kelas dan yang memiliki kedekatan lebih dari 90% ada tiga kelas
Effect of Microwave Treatment on the Profile of Volatile Compounds and Characteristics of White Pepper (Piper nigrum L.) Essential Oil
Full text linkMicrowave treatment on white pepper was conducted to damage cell tissue to facilitate the distillation and increase the yield of essential oils. The research objective was to determine the effect of pepper varieties and microwave treatment on the profiles of volatile compounds and the characteristics of white pepper essential oils. The research was conducted with 50 grams of white pepper placed into a 15 cm diameter petri dish and put in a microwave oven at the power of 600 watts for 90 seconds then white pepper milled by hammer mill. Essential oils were obtained using the water distillation method then were tested for their characteristics and compounds using Gas chromatography-Mass Spectrometry (GC-MS). White pepper was analyzed using the water content, piperine content, and its cell tissue microstructure was analyzed using Scanning Electron Microscope (SEM). The results showed that different pepper as accession produced different profiles of volatile compounds, characteristics, and antioxidant activity (p<0.05). The number of volatile compounds of white pepper essential oils identified by GC-MS was 42, where the largest compound was β-caryophyllene (50.51%). Microwave treatment damaged the cell wall of white pepper, lowered water content (17.43%), increased piperine content (10.57%) and essential oil yield (25%), and changed the profiles of volatile compounds of essential oils of Lampung daun lebar accession. Color became bluer (b*value rises by 201.99%), increased specific gravity (1.27%) and antioxidant activity (7.4%), decreased solubility in 95% ethanol (17.95%) and acid number (20.8%) while the refractive index was not affected by pepper accessions and microwave treatment.Microwave treatment on white pepper was conducted to damage cell tissue to facilitate the distillation and increase the yield of essential oils. The research objective was to determine the effect of pepper varieties and microwave treatment on the profiles of volatile compounds and the characteristics of white pepper essential oils. The research was conducted with 50 grams of white pepper placed into a 15 cm diameter petri dish and put in a microwave oven at the power of 600 watts for 90 seconds then white pepper milled by hammer mill. Essential oils were obtained using the water distillation method then were tested for their characteristics and compounds using Gas chromatography-Mass Spectrometry (GC-MS). White pepper was analyzed using the water content, piperine content, and its cell tissue microstructure was analyzed using Scanning Electron Microscope (SEM). The results showed that different pepper as accession produced different profiles of volatile compounds, characteristics, and antioxidant activity (p<0.05). The number of volatile compounds of white pepper essential oils identified by GC-MS was 42, where the largest compound was β-caryophyllene (50.51%). Microwave treatment damaged the cell wall of white pepper, lowered water content (17.43%), increased piperine content (10.57%) and essential oil yield (25%), and changed the profiles of volatile compounds of essential oils of Lampung daun lebar accession. Color became bluer (b*value rises by 201.99%), increased specific gravity (1.27%) and antioxidant activity (7.4%), decreased solubility in 95% ethanol (17.95%) and acid number (20.8%) while the refractive index was not affected by pepper accessions and microwave treatment
Pengaruh Lama dan Suhu Pengeringan Terhadap Karakteristik Fisikokimia Tepung Beras Merah (Oryza nivara): Pengaruh Lama dan Suhu Pengeringan Terhadap Karakteristik Fisikokimia Tepung Beras Merah (Oryza nivara)
No full textRed rice is known to have advantages, i.e. contain fiber and anthocyanin that have functional properties. The potential of red rice needs to be explored more intensively through various studies. One effort to develop brown rice as food is to process it into flour. In the process of flour processing, there are several steps that must be considered to get flour with good physicochemical properties, such as drying and grinding. The purpose of this research was to learn the effect of drying time and temperature on the physicochemical characteristics of red rice flour. Randomized Block Design factorial was used in this research. The treatment factors were drying time (A) and drying temperature (B). Factor A consisted of three levels i.e. 1 hour, 2 hours, and 3 hours, and factor B also consisted of three levels i.e. 50°C, 55°C, and 60°C. The parameters observed in this study were yield, bulk density, water holding capacity, swelling power, solubility, moisture content, and amylose content of red rice flour. The results showed that the drying times and temperatures significantly reduced yield, bulk density, and moisture content, but significantly increased water holding capacity, swelling power, solubility, and amylose content of red rice flour. The moisture content of red rice flour was in accordance with the standards (SNI 3549; 2009).Beras merah diketahui memiliki kelebihan, yaitu mengandung serat dan antosianin yang bersifat fungsional. Potensi beras merah perlu digali lebih intensif melalui berbagai penelitian. Salah satu upaya pengembangan beras merah sebagai bahan pangan adalah dengan cara mengolahnya menjadi tepung. Terdapat beberapa tahapan yang harus diperhatikan untuk mendapatkan tepung dengan sifat fisikokimia yang baik, seperti pengeringan dan penggilingan. Penelitian ini bertujuan untuk mempelajari pengaruh lama dan suhu pengeringan terhadap karakteristik fisikokimia tepung beras merah. Penelitian ini menggunakan Rancangan Acak Kelompok faktorial. Faktor penelitian yaitu lama pengeringan (A) dan suhu pengeringan (B). Faktor A terdiri dari tiga taraf yaitu 1 jam, 2 jam, dan 3 jam, dan faktor B juga terdiri dari tiga taraf yaitu 50°C, 55°C, dan 60°C. Parameter yang diamati adalah rendemen, densitas kamba, kapasitas penyerapan air, swelling power, kelarutan, kadar air, dan kadar amilosa tepung beras merah. Hasil penelitian menunjukkan bahwa lama dan suhu pengeringan secara signifikan menurunkan rendemen, densitas kamba, dan kadar air, tetapi secara signifikan meningkatkan kapasitas penyerapan air, swelling power, kelarutan, dan kadar amilosa tepung beras merah. Kadar air tepung beras merah yang dihasilkan telah sesuai dengan standar SNI (3549; 2009)
Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.): Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)
No full textTorbangun (Plectranthus amboinicus) is a medicinal plant belonging to the Lamiaceae family. In Batak society, the torbangun leaves are consumed to increase breast milk production. The phytochemical variability can be improved by gamma-ray irradiation for plant breeding programs. In this work, a non-targeted metabolomics method was followed to evaluate the compounds profile of irradiated mutant and wild-type on leaves of P. amboinicus. GC-MS analyses evaluated the mutant putative (derived from gamma-ray irradiation) and wild-type plantlets. The resulting of GC-MS data were analyzed with chemometric analysis using hierarchical cluster analysis (HCA). The chemical diversity was showed in mutant and wild-type P. amboinicus. The major metabolite in plantlet wild-type were hydroxymethylfurfural (35.15%), brevifolin (7.69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13.13%), stigmasterol (6.51%) and ferruginol (8.63%). However, the compound of DDMP (19.57%), neophytadiene (8.47%), linolenic acid (9.10%), ferruginol (7.61%), stigmasterol (14.14%) and gamma-sitosterol (7.08%) were identified in mutant plantlet. HCA showed diversity between wild-type and mutant plantlets, which highest content of DDMP and stigmasterol and lowest of hydroxymethylfurfural of mutant plantlet.Torbangun (Plectranthus amboinicus) merupakan tanaman obat dari keluarga Lamiaceae. Pada masyarakat Batak, daun torbangun digunakan untuk meningkatkan produksi air susu. Keragaman kandungan fitokimia dapat ditingkatkan salah satunya dengan teknik iradiasi sinar gamma untuk program pemuliaan tanaman. Pada penelitian ini, metode metabolomik yang tidak tertarget digunakan untuk mengevaluasi profil senyawa pada bagian daun tanaman mutan dari iradiasi sinar gamma dan kontrol. Kandungan senyawa planlet mutan dan kontrol dianalisis dengan GC-MS. Data GC-MS dianalisis dengan kemometrik dengan menggunakan hierarchical cluster analysis (HCA). Keragaman kandungan kimia ditunjukkan oleh planlet mutan dan kontrol daun torbangun. Hasil analisa planlet kontrol torbangun menunjukkan terdapat lima senyawa utama diantaranya hydroxymethylfurfurole (35,15%), brevifolin (7,69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13,13%), stigmasterol (6,51%) dan ferruginol (8.63%). Hasil analisa mutan torbangun memiliki kandungan senyawa DDMP (19,57%), neophytadiene (8,47%), linolenic acid (9,10%), ferruginol (7,61%), stigmasterol (14,14%) dan gamma.-sitosterol (7,08%). HCA menunjukkan tiga komponen senyawa yang berbeda antara planlet kontrol dan mutan yaitu kenaikan kandungan senyawa DDMP dan stigmasterol, serta penurunan kandungan hydroxymethylfurfurole pada mutan torbangun