Medical Laboratory Analysis and Sciences Journal (Melysa)
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The incidence of onychomycosis in farmers in Krajan Hamlet, Beji Village, Boyolangu District, Tulungagung Regency
No full textOnychomycosis is a nail disorder caused by fungal infections, both by dermatophytes, non-dermatophytes, and yeast with symptoms of damage to the nails, including the nails become thicker, cracked, uneven, not shiny, and there is a change in nail color to white, yellow, brown, to black. Onychomycosis can occur in farmers due to working directly in wet, damp and dirty places. This study aims to determine the incidence of onychomycosis in farmers in krajan Hamlet, Beji Village, Boyolangu District, Tulungagung Regency. This study is a descriptive study with Purposive Sampling sampling techniques and samples as many as 15 farmers who meet the criteria, examination of fungi using culture and microscopic methods on fungal colonies. The results showed that all respondents farmers infected with onychomycosis (100%) with 9 species of fungi found Trichophyton rubrum (21.43%), Rhizopus sp (21.43%), Penicillium sp (14.30%), Trichophyton mentagrophytes (10.71%), Trichophyton tonsurans (10.71%), Aspergillus niger (7.14%), Mucor sp (7.14%), Aspergillus flavus (3.57%), and Microsporum canis (3.57%). The presence of the fungus is due to the habit of farmers who do not wear personal protective equipment, do not regularly wash their hands and feet using soap, and pay less attention to nail hygiene. The conclusion of this study the incidence of onychomycosis in Farmers in Hamlet inKrajan Village Beji District Boyolangu Tulungagung Regency is very high as many as 15 people (100%). It is expected that farmers pay attention to nail hygiene, wash their hands and feet with soap, and wear personal protective equipment while working.Onikomikosis adalah kelainan kuku yang disebabkan oleh infeksi jamur, baik oleh dermatofita, non dermatofita, maupun ragi dengan gejala kerusakan kuku, antara lain kuku menjadi lebih tebal, pecah-pecah, tidak rata, tidak mengkilat, dan terdapat perubahan warna kuku menjadi putih, kuning, coklat, hingga hitam. Onikomikosis dapat terjadi pada petani karena bekerja langsung di tempat yang basah, lembab dan kotor. Penelitian ini bertujuan untuk mengetahui kejadian onikomikosis pada petani di Dusun krajan Desa Beji Kecamatan Boyolangu Kabupaten Tulungagung. Penelitian ini merupakan penelitian deskriptif dengan teknik Purposive Sampling dan sampel sebanyak 15 petani yang memenuhi kriteria, pemeriksaan jamur menggunakan metode kultur dan mikroskopis pada koloni jamur. Hasil penelitian menunjukkan bahwa semua petani responden terinfeksi onikomikosis (100%) dengan 9 jenis jamur ditemukan Trichophyton rubrum (21,43%), Rhizopus sp (21,43%), Penicillium sp (14,30%), Trichophyton mentagrophytes (10,71%), Trichophyton tonurans (10,71%), Aspergillus niger (7,14%), Mucor sp (7,14%), Aspergillus flavus (3,57%), dan Microsporum canis (3,57%). Adanya jamur tersebut dikarenakan kebiasaan petani yang tidak memakai alat pelindung diri, tidak rutin mencuci tangan dan kaki menggunakan sabun, serta kurang memperhatikan kebersihan kuku. Kesimpulan dari penelitian ini angka kejadian onikomikosis pada Petani di Dusun Desa Krajan Kecamatan Beji Kabupaten Boyolangu Kabupaten Tulungagung sangat tinggi yaitu sebanyak 15 orang (100%). Diharapkan petani memperhatikan kebersihan kuku, mencuci tangan dan kaki dengan sabun, dan memakai alat pelindung diri saat bekerja
GAMBARAN C-REAKTIF PROTEIN (CRP) METODE AGLUTINASI LATEX PADA SIKLUS MENSTRUASI MAHASISWI STIKES HUTAMA ABDI HUSADA TULUNGAGUNG TAHUN 2021
No full textC-Reactive Protein (CRP) is an acute phase protein that is present in normal serum even in small amounts. In certain circumstances with an inflammatory reaction or tissue damage (necrosis) caused by infectious and non-infectious diseases. One of the causes of inflammation is menstruation. Menstruation is periodic bleeding in the uterus starting about 14 days of ovulation. This study aimed to determine CRP levels in the menstrual cycle of STIKes Hutama Abdi Husada Tulungagung students in 2021. This type of research is descriptive with the number of respondents 17 female students. The samples in this study were 17 serum samples of students from the Medical Laboratory Technology Study Program of STIKes Hutama Abdi Husada Tulungagung using the Purposive Sampling technique with data analysis using percent (%) to determine whether or not there was agglutination in the serum of female students who were menstruating. The results of this study are presented in tabular form. In the qualitative examination, the agglutination was positive with a percentage of 6% and negative agglutination with a percentage of 94% in the serum samples of the STIKes Hutama Abdi Husada Tulungagung student in 2021. Based on the results of this study, it can be said that there is no increase in CRP levels in the menstrual cycle of STIKes Hutama Abdi Husada Tulungagung students in 2021
The effect of Lactobacillus plantarum and Lactobacillus acidhophilus on cow milk and soy milk fermented
No full textThis study aims to determine the effect of Lactobacillus Plantarum and Lactobacillus Acidhophilus Bacteria on Probiotic Beverage (Yogurt) from Cow\u27s Milk and Soy\u27s Milk, making yogurt is done by randomized design of groups factorially arranged using Lactobacillus plantarum. The second factor is the proportion of cow\u27s milk and soy milk served at, namely: 100% cow\u27s milk (S1), 50% cow\u27s milk and 50% soy milk (S2) and 100% soy milk (S3) with the treatment applied through, addition lactic acid and viscystas from yogurt. Analysis results obtained from the addition of lactic acid. The proportion of cow\u27s milk and soybean milk showed that Lactobacillus plantarum obtained a total of Lactic Acid of 0.45 and Lactobacillus acidhophilus. The proportion of Cow\u27s Milk and Soymilk obtained a total Lactic Acid of 0.35. And the results of the analysis obtained from the Viscosity Proportion of Cow\u27s Milk and Soybean Milk show that Lactobacillus plantarum obtained a total viscosity of 1.5 and Lactobacillus acidhophilus Proportion of Cow\u27s Milk and Soybean Milk obtained a viscosity of 2.9.Keywords: influence, lactobacillus plantarum and lactobacillus acidhophilus, cow\u27s milk and soy mil
Levels of Cadmium (Cd) and Copper (Cu) in the Blood of Rice Farmers in Pucung Kidul Village, Boyolangu District Tulungagung Regency
No full textFarmers in Pucung Kidul Village often use pesticides to eradicate pests, diseases, and weeds on plants. Pesticides contain several kinds of metals, including cadmium (Cd) and copper (Cu). High levels of cadmium in the blood can interfere with the process of erythropoiesis and heme synthesis caused by the accumulation of cadmium. While copper poisoning can cause degenerative processes in the brain and cirrhosis of the liver. This research is a descriptive study that aims to measure the levels of cadmium and copper in the blood of farmers in Pucung Kidul Village. Measurements were made using atomic absorption spectroscopy (AAS). The sample used in this study was 15 samples obtained by the purposive sampling technique. The results showed that all respondents did not use PPE and 2 respondents with a working period of > 5 years obtained cadmium levels from 15 samples, there was 1 sample with cadmium levels above the threshold, which was 0.047 mg/L. Meanwhile, the copper content of the 15 samples contained 1 sample with the copper content above the threshold, which was 1.27 mg/L. This shows that the proper use of PPE, also years of service, and age can affect blood levels of cadmium and copper. Where the longer the working period, the more exposure that occurs and causes high levels of cadmium and copper in the blood. With the results of this study, it is hoped that farmers will continue to use personal protective equipment (PPE) when using pesticides and wash their hands after using them.Petani di Desa Pucung Kidul sering menggunakan pestisida untuk memberantas hama, penyakit dan gulma pada tanaman. Pestisida mengandung beberapa macam logam, di antaranya kadmium (Cd) dan tembaga (Cu). Kadar kadmium yang tinggi dalam darah dapat mengganggu proses eritropoiesis dan sintesis heme yang diakibatkan oleh akumulasi kadmium. Sedangkan keracunan tembaga dapat menyebabkan terjadinya proses degeneratif pada otak dan sirosis pada hati. Penelitian ini merupakan penelitian deskriptif yang bertujuan untuk mengukur kadar kadmium dan tembaga dalam darah petani di Desa Pucung Kidul. Pengukuran dilakukan menggunakan spektroskopi serapan atom (SSA). Sampel yang digunakan dalam penelitian ini sebanyak 15 sampel yang diperoleh dengan teknik Purposive Sampling. Hasil penelitian menunjukkan kadar kadmium dari 15 sampel terdapat 1 sampel dengan kadar kadmium di atas ambang batas, yaitu sebesar 0,047 mg/L. Sedangkan kadar tembaga dari 15 sampel terdapat 1 sampel dengan kadar tembaga di atas ambang batas, yaitu sebesar 1,27 mg/L. Di mana dari penelitian tersebut diketahui seluruh responden tidak menggunakan alat pelindung diri (APD) dengan benar. Hal tersebut menunjukkan bahwa penggunaan APD secara tepat, juga masa kerja, dan usia dapat memengaruhi kadar kadmium dan tembaga dalam darah. Di mana semakin lama masa kerja, maka semakin banyak paparan yang terjadi dan menimbulkan tingginya kadar kadmium dan tembaga dalam darah. Dengan adanya hasil penelitian ini diharapkan para petani tetap menggunakan alat pelindung diri (APD) saat menggunakan pestisida dan mencuci tangan setelah menggunakannya
Analyze of formalin in kolang kaling (Arenga pinnata Merr) and black grass jelly (Mesona palustris BL)
Get PDFFormalin is a chemical toxic that is abused as a food preservative and prohibited by the Indonesian government. Thus, there is a need for continuous analysis of foodstuffs that are sold in the market. When food containing formaldehyde is consumed, it will interfere with the metabolism of organs and body systems. This study aimed to analyze the presence and absence of formalin content on Kolang Kaling (Arenga pinnata Merr) and Black Grass Jelly (Mesona palustris BL) sold in Samarinda City Traditional Market, especially Segiri market, Pagi market, and Sungai Dama market. The test of formalin content was performed qualitatively using chromatophilic acid and continued quantitatively using UV-Vis spectrophotometry at the Biochemical MIPA laboratory. The results showed that 9 samples from 18 samples were identified using formalin as a preservative. The levels of formalin obtained were different, the lowest formalin level of 0.0240 mL / L in the Cincau samples (Mesona palustris BL) obtained in the Segiri market and the highest formalin level of 0.0531 mL / L in the kolang kaling sample (Arenga pinnata Merr) obtained on the Pagi market
The protein levels changes due to the frying process in egg and tempeh
No full textFood is a basic need as a source of energy for humans. Food components needed by humans are consist of several constituent substances including carbohydrates, fats, vitamins, minerals, water, and protein. The most widely consumed protein sources by Indonesian people are eggs and tempeh. Processing eggs and tempeh in order to have a good taste is usually done by heating through the frying process. The frying process requires temperatures between 160-300oC. The high temperature when frying can cause protein damage so that it cannot be utilized by the human body. Therefore it is necessary to research changes in protein levels in eggs and tempeh due to heating through the frying process. The research method used was spectrophotometry with the complexing agent used was a biuret reagent. The result showed that there was decreasing content on both egg and tempeh due to the frying process. A decrease in protein content was 29.21 mg or 23.28% in eggs. Whereas in tempeh samples there was a decrease in protein content by 59.03 mg or 49.33%. Therefore the heating process in egg and tempeh should be done under 160o
Antibacterial activity of combined extracts of red ginger (Zingiber officinale var. Rubrum) and betel leaf (Piper betle L.) against Staphylococcus aureus and Escherichia coli
Get PDFThe use of traditional medicine can be an alternative treatment with increasing antibiotic resistance. Red ginger (Zingiber officinale var. Rubrum) and betel leaf (Piper betel L.) are plants that can be used as an antibacterial. This study aimed to determine the antibacterial activity of combined extracts of red ginger and betel leaf. This experiment was carried out by making extracts of red ginger and betel leaf with maceration and tested the antibacterial activity. The antibacterial activity test used the paper disc diffusion method. The study was conducted with five different concentrations of extract 20%, 40%, 60%, 80%, and 100%. The results showed that the combination of red ginger and betel leaf extract at a concentration of 80% had the highest inhibitory area against Staphylococcus aureus (10.6 mm) and Escherichia coli (7.5 mm). The data obtained were statistically tested using One Way Anova with a significance level of > 0.05. The results of One Way ANOVA analysis of the combination of red ginger and betel leaf extracts on Staphylococcus aureus had a significant value of 0.083, while those extracts on Escherichia coli had a significant value of 0.690. It can be concluded that the combination of red ginger and betel leaf extract at a concentration of 80% had a strong inhibitory level against Staphylococcus aureus and a moderate inhibitory level against Escherichia coli
The activity of ethanol extract and aquadest of Jati Belanda (Guazuma ulmifolia Lamk) leaves against Candida albicans
Get PDFFungi are the cause of infection, the development of fungal infections in Indonesia is influenced by environmental conditions so that microbes grow quickly and can increase significantly. Candida Albicans is a fungal disease that infects humans. As a country with a tropical climate, Indonesia has potential biodiversity as a medicine, one of which is Dutch teak (Guazuma ulmifolia Lamk) which has secondary metabolites that kill microbes that can be used as natural antifungals. The purpose of this study was to determine the antifungal activity of ethanol extract and distilled water of Dutch teak leaves on the growth of Candida Albicans. The method used is maceration with ethanol and distilled water solvents. The anti-fungal activity was evaluated by the good diffusion method with SDA media. The results of the antifungal activity of ethanol extract and distilled water of Dutch teak leaves against Candida Albicans were indicated by the formation of an inhibition zone around the wells on Candida Albicans ethanol extract concentrations of 25 mg / mL to 1000 mg / mL 8.6 mm - 15.6 mm, the distilled water extract has not formed a zone of inhibition at all concentrations. The conclusion is that the ethanol extract of Dutch teak leaves can inhibit the growth of Candida Albicans. while the distilled water extract was unable to inhibit the growth of Candida Albicans.Jamur merupakan penyebab infeksi, perkembangan infeksi jamur di Indonesia dipengaruhi keadaan lingkungan sehingga mikroba tumbuh dengan cepat dan dapat meningkat secara signifikan. Candida albican merupakan penyakit jamur yang menginfeksi manusia. Sebagai negara yang beriklim tropis Indonesia memiliki keanekaragaman hayati berpotensi sebagai obat salah satunya adalah jati belanda (Guazuma ulmifolia Lamk) yang memiliki senyawa metabolit sekunder mampu membunuh mikroba yang dapat digunakan sebagain antijamur alami. Tujuan dari penelitian ini adalah untuk mengetahui Aktivitas Antifungi Ekstrak etanol dan aquades daun jati belanda terhadap pertumbuhan Candida albican. Metode yang digunakan adalah maserasi dengan pelarut etanol dan aquades. Aktivitas antifungi dievaluasi dengan metode difusi sumuran dengan media SDA. Hasil aktivitas antifungi ekstrak etanol dan aquades daun jati belanda terhadap Candida albican ditandai dengan terbentuknya zona hambat disekitar sumuran pada Candida albican ekstrak etanol konsentrasi 25 mg/mL sampai 1000 mg/mL 8,6 mm - 15,6 mm, pada ekstrak aquades tidak terbentuk zona hambat pada semua konsentrasi. Kesimpulan pada ekstrak etanol daun jati belanda mampu penghambat pertumbuhan Candida albican. sedangkan pada ekstrak aquades tidak mampu menghambat pertumbuhan Candida albican
In silico studi of Orthosiphon aristatus as antioxidant
Get PDFOrthosiphon aristatus (O. aristatus) is a herbal plant that has antioxidant activity. This study was conducted in a preliminary study of the potential active compound O. aristatus with in silico software pyrex autodock vina method, and visualization using biovia discovery studio. Results from the study in silico showed that O.aristatus extract has potential as an antioxidant compound. Result value of binding affinity with SOD receptor proteins having more negative values compared to control compounds. The interaction of O.aristatus extract is quite potential as an antioxidant, through hydrogen bonding that is equal to control, namely vitamin EOrthosiphon aristatus (O. aristatus) adalah tanaman herbal yang memiliki aktivitas antioksidan. Penelitian ini merupakan studi awal tentang senyawa aktif potensial O. aristatus dengan metode in silico pyrex autodock vina, dan visualisasi menggunakan biovia discovery studio. Hasil dari penelitian di silico menunjukkan bahwa ekstrak O.aristatus memiliki potensi sebagai senyawa antioksidan. Nilai hasil afinitas pengikatan dengan protein reseptor SOD memiliki nilai yang lebih negatif dibandingkan dengan senyawa kontrol. Interaksi ekstrak O.aristatus cukup potensial sebagai antioksidan, melalui ikatan hidrogen yang sama dengan kontrol, yaitu vitamin E
Physical, chemical, and biological analysis of drinking water quality in Tulungagung regency, East Java
Get PDFThis study aims to analyze the quality of water in Tulungagung. Identification carried out is a physical test including pH, salinity, color, and temperature. As well as chemical tests, including alkalinity test, CO2 content, Cl, and Water Hardness. The sample water collected from six locations in Tulungagung Regency which are Sendang, Rejotangan, Campurdarat, Keras, Ngunut, and Tanggunggunung. From the pH test was obtained that all the sample water qualifies as good water (pH 6.5 to 8.0). The content of CO2 water samples shows that result with the highest CO2 21 ppm and the lowest 18ppm. Whereas the Cl test obtained the highest 135.3 ppm and the lowest was 52.7 ppm This analysis data was enriched with hardness test results with the highest hardness 60.9 D while the lowest hardness water was 20.9