Dokkyo Medical University Repository / 獨協医科大学リポジトリ
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Claudin 18.2 Inhibits the Proliferation of Small-Cell Lung Cancer Cells via G0/G1 Cell-Cycle Arrest
Get PDFClaudin (CLDN) 18.2, a member of the CLDN family, contains tight junctions that regulate the physiological functions of epithelial cells, such as cell differentiation, proliferation, and polarity. CLDN18.2 is also expressed, and performs various functions, in several cancers; however, its expression and function in small-cell lung cancer (SCLC) have not been elucidated. Herein, we determined the expression of CLDN18.2 in SCLC cell lines using quantitative RT-PCR and western blotting. Moreover, we established inducible CLDN18.2 expression SCLC cells and CLDN18.2 knockout SCLC cells and showed that CLDN18.2 inhibited the proliferation of SCLC via G0/G1 cell-cycle arrest using cell-proliferation assay and cell-cycle analysis. This cell-cycle arrest was caused by downregulation of CCND1 and induction of CDKN1A. The present study evokes research interest in CLDN18.2 as a potential therapeutic target and prognostic marker in SCLC, paving the way for future research prospects.journal articl
A Case of Multisystem Inflammatory Syndrome in Children Diagnosed Based on Gastrointestinal Symptoms Despite Not Meeting the Diagnostic Criteria for Kawasaki Disease
Get PDFSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is usually mild in children. However, a condition known as "multisystem inflammatory syndrome in children" (MIS-C) has recently attracted attention. Typically, MIS-C develops 2-6 weeks after acute respiratory SARS-CoV-2 infection, and the affected patients present with gastrointestinal symptoms and cardiovascular dysfunction in addition to Kawasaki disease (KD)-like features. A six-year-old boy was admitted to our hospital with a 2-day history of fever, abdominal pain, vomiting, and tender cervical lymph node swelling. The patient was found to be negative for acute appendicitis and bacterial enteritis. Although he did not meet the diagnostic criteria for KD, the presence of abdominal symptoms and laboratory evidence of inflammation, a history of SARS-CoV-2 infection in family members living in the same household, and positive results from the new coronavirus antibody test made it possible to diagnose MIS-C. Interleukin-6 was high at admission but decreased with fever resolution after treatment. All pediatricians and pediatric surgeons should consider the possibility of MIS-C when encountering patients with gastrointestinal symptoms after SARS-CoV-2 infection or after intense contact, even in pediatric patients who do not fulfill the diagnostic criteria for KD.journal articl
Novel Variants in AARS1-related White Matter Disease: A Case Report
Get PDFWe report a case of 1-year-old Japanese girl exhibiting severe developmental delay, microcephaly, gastroesophageal reflux, and failure to thrive. Whole exome sequencing revealed likely pathogenic, novel compound heterozygous AARS1 missense variants, inherited from her parents. In cases of severe developmental delay with white matter abnormalities and feeding difficulties, AARS1 abnormalities should be listed as a differential disease.journal articl
