16144 research outputs found
Sort by
The Anchor, Volume 132.23: April 17, 2019
Get PDFThe Anchor began in 1887 and was first issued weekly in 1914. Covering national and campus news alike, Hope College’s student-run newspaper has grown over the years to encompass over two-dozen editors, reporters, and staff. For much of The Anchor\u27s history, the latest issue was distributed across campus each Wednesday throughout the academic school year (with few exceptions). In recent years The Anchor moved to monthly print issues and a more frequently updated website. The Anchor is now published in print twice per academic semester. Occasionally, the volume and/or issue numbering is irregular
Use of Site-Directed Mutagenesis to Probe the Cystine Binding Site within System xc-
Get PDFThe Chase lab studies System xc-, which is a heterodimeric transporter that functions as a Na+-independent antiporter exchanging extracellular cystine for intracellular glutamate. The transporter functions as an obligate heterodimer and is comprised of two proteins, xCT, which functions specifically in amino acid exchange, and 4F2HC, which appears to play a role in transporter stability. System xc- belongs to the SLC7 family of transporters, which is further subdivided into the cationic amino acid transporters (CAT) and the L-type amino acid transporters (LAT), the latter of which is the subfamily which includes System xc-. In recent years, there have been advances in our understanding of the structure of the SLC7 transporters as crystal structures of bacterial homologs of CAT and LAT transporters have been published. As a result, we can now begin to ask important questions about the mechanism by which System xc- exchanges cystine and glutamate across the membrane. Previous studies in the Chase lab have demonstrated that System xc- is a Cl--dependent transporter that transports cystine and glutamate in their anionic form. This substrate specificity is novel among the SLC7 family of transporters, therefore, the goal of this study is to identify the amino acids within xCT that are important in Cl--binding and substrate binding and exchange. Previous studies have suggested that Cys 327 is important for transport activity and this residue is also conserved in the bacterial homolog. Therefore, we selected amino acids near cysteine 327 and within the apparent binding pocket for mutagenesis. Primers were designed to create the point mutations at S326A, T195A, F322A, Y149A, S322A, V141S and M331A, and we intend to test these mutants for transport activity. We hypothesize that mutants which lead to diminished transport activity or changes in substrate affinity are likely to be important in Cl--binding, substrate binding and/or translocation
Stage Management for Cry It Out
Get PDFOver the course of seven weeks, the Hope College Theatre Department produced the play Cry It Out by Molly Smith Metzler. The stage management team led each rehearsal and created rehearsal schedules and reports. This information was then synthesized and communicated with the design and production teams, then later reviewed and discussed in production meetings. As the play moved from standard rehearsals into technical rehearsals, the stage manager oversaw all the backstage crews and technicians. In performance, the stage manager called every lighting and sound cue, while keeping an open line of communication with the Assistant Stage Managers, Light Board Operator, Sound Board Operator, and House Manager. Through the work of the stage manager, the cast, director, and production team is able to easily communicate with one another throughout the rehearsal process, and, in turn, the performances of the production run smoothly
Keep \u27Em Fighting: Chris-Craft\u27s Contribution to WWII
Get PDFOn June 6, 1944, Allied forces clamored over the sides of large troop ships into 4126 landing craft, many of those LCVPs (Landing Craft, Vehicle, Personnel), and prepared to storm several beaches of northern France in Operation Overlord, better known as D-Day. Preparation for this day started in the early 1942 as companies throughout the United States converted their factories from peacetime to wartime production. In Michigan, all three Chris-Craft Corporation plants quickly converted from pleasure boat building to building boats for the war effort full time. Together, the three plants would become part of the America’s arsenal of democracy from 1941-1945 producing more than 12,000 landing craft for D-Day and other invasions
Hope College Abstracts: 18th Annual Celebration of Undergraduate Research and Creative Activity
Get PDFThe 18th Annual Celebration of Undergraduate Research and Creative Activity was held on April 12, 2019 in the Richard and Helen DeVos Fieldhouse at Hope College and featured student-faculty collaborative research projects. This program is a record reflective of those projects between the 2018-2019 academic year
Analysis of Surgical Personnel Traffic in the Operating Room
Get PDFOperating rooms (ORs) are one of the most aseptic environments in the hospital, but factors such as traffic caused by surgical personnel are associated with surgical site infections. This study proposes to evaluate the mean number of door swings per case among those trafficking the OR and reasons for entry and exit. This study will apply Florence Nightingale’s Environmental Theory, as this theory focuses on factors within the environment that can affect the patient’s outcome which is the main objective of this study. This observational study comes from log data (n= 71) of monitored scheduled surgical cases including the time, which door was used, direction of entry/exit, the personnel responsible, the reason for entry or exit of the OR, and number of doors swings total for each case at a non-profit Midwest hospital. Data analysis using descriptive statistics was conducted with SPSS. Limitations include the awareness of surgical personnel (Hawthorne effect), contradictions between data documentation, and technical limitations within the collection tools. The analysis of the data indicated a large percentage of OR traffic due to the role of the circulating RN (20%), surgical techs (17%), and OR assistants (10%). The greatest percentage of OR traffic was due to unknown reasons (33%). These results indicate the need to expand reasons for entry and exit in the observation tool. Further studies may reveal behaviors within the OR nurse population that may need to be altered in addition to ways nurses can encourage other surgical personnel to monitor their trafficking behaviors
News from Hope College, Volume 50.3: Spring, 2019
Get PDFhttps://digitalcommons.hope.edu/news_from_hope_college/1250/thumbnail.jp
Let it Rest: Sleep and Health as Positive Correlates of Forgiveness of Others and Self-forgiveness
Get PDFObjective: The present study examined forgiveness of others, self-forgiveness, sleep, and health in a nationally representative sample of United States adults. It was hypothesised that sleep would mediate the associations of forgiveness of others and self-forgiveness with health.
Design: A nationally representative survey of 1,423 United States adults.
Main Outcome Measures: Measures included forgiveness of others, self-forgiveness, sleep quantity, sleep quality, psychological distress, life satisfaction, and self-rated physical health.
Results: Forgiveness of others (β = .20, p \u3c .001) and self-forgiveness (β = .11, p \u3c .01) were associated with sleep and forgiveness of others (β = .24, p \u3c .001) and self-forgiveness (β = .27, p \u3c .001) were associated with health. Sleep was associated with health (β = .45, p \u3c .001) and also acted as a mediator of the associations of forgiveness of others (β = .09, p \u3c .01) and self-forgiveness (β = .05, p \u3c .01) with health.
Conclusions: Forgiveness of others and self-forgiveness may attenuate emotions such as anger, regret, and rumination and provide a buffer between one’s own and others’ offenses occurring during the day and offer a restful mental state that supports sound sleep which, in turn, is associated with better health
β-decay Half-lives of Neutron-rich Nuclides in the A = 100 – 110 Mass Region
Get PDFβ-decay half-lives of neutron-rich nuclides in the A = 100–110 mass region have been measured using an implantation station installed inside of the Summing NaI(Tl) (SuN) detector at the National Superconducting Cyclotron Laboratory. Accurate half-lives for these nuclides are important for nuclear astrophysics, nuclear structure, and nuclear technology. The half-lives from the present work are compared with previous measurements, showing overall good agreement