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Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes
Get PDFThe sorting and assembly machinery (SAM) Complex is responsible for assembling β‐barrel proteins in the mitochondrial membrane. Comprising three subunits, Sam35, Sam37, and Sam50, the SAM complex connects the inner and outer mitochondrial membranes by interacting with the mitochondrial contact site and cristae organizing system complex. Sam50, in particular, stabilizes the mitochondrial intermembrane space
bridging (MIB) complex, which is crucial for protein transport, respiratory chain complex assembly, and regulation of cristae integrity. While the role of Sam50 in mitochondrial structure and metabolism in skeletal muscle remains unclear, this study aims to investigate its impact. Serial block‐face‐scanning electron microscopy and computer‐assisted 3D renderings were employed to compare mitochondrial structure and networking in Sam50‐ deficient myotubes from mice and humans with wild‐type (WT) myotubes. Furthermore, autophagosome 3D structure was assessed in human myotubes. Mitochondrial metabolic phenotypes were assessed using Gas Chromatography‐Mass Spectrometry‐based metabolomics to explore differential changes in WT and Sam50‐deficient myotubes. The results revealed increased mitochondrial fragmentation and autophagosome formation in Sam50‐deficient myotubes compared to controls. Metabolomic analysis indicated elevated metabolism of propanoate and several amino acids, including ß‐ Alanine, phenylalanine, and tyrosine, along with increased amino acid and fatty acid metabolism in Sam50‐deficient myotubes. Furthermore, impairment of oxidative capacity was observed upon Sam50 ablation in both murine and human myotubes, as measured with the XF24 Seahorse Analyzer. Collectively, these findings support the critical role of Sam50 in establishing and maintaining mitochondrial integrity, cristae structure, and mitochondrial metabolism. By elucidating the impact of Sam50‐deficiency, this study enhances our understanding of mitochondrial function in skeletal muscle
Perceptions of School Leaders About Digital Communication in the Saudi Education System
No full textThe purpose of this study was to determine the perceptions of school leaders in Bisha about digital communication in the Saudi education system. The purpose of the current study was also to identify the attitudes of school leaders towards digital communication in terms of factors affecting the acceptance and use of digital communication in the educational process, and to identify the extent of performance and the expected benefit from the use of digital communication systems by school leaders in the Saudi educational system. This study also attempted to test the impact of some factors, such as ease of use, perceived usefulness, and behavioral intention in the actual use of digital communication technologies, and to reveal the obstacles facing school leaders in the Saudi education system while using digital communication technologies, and their suggestions for developing the use of these technologies in the educational process. One of the key findings of the study is that the presence of high levels of satisfaction among school leaders in the Saudi education system with the use of digital communication. They emphasized the importance of focusing on training courses to enhance the technical capabilities of school leaders, teachers, and students. Importantly, the study revealed that there were no statistically significant differences based on educational qualifications, leadership experience, or participation in training courses, indicating a consistent trend of positive attitudes toward digital communication among school leaders
Nutritional Requirements of Solanum lycopersicum, Vaccinium corymbosum, Citrus hystrix and Rubus idaeus
No full textPlant nutrient management has gained significant attention due to environmental concerns, the need to sustainably feed a growing population, the misuse of nonrenewable resources, and the rise of container-grown crops in soilless media. However, there is a lack of specific information available for different crops. Investigations were conducted on cherry tomato, blueberry, kaffir lime, and raspberry to explore the impact of supplied macro and micronutrient sources, rates, and their interactions on plant growth and development. The focus was on the effects of nitrogen, phosphorus, potassium, calcium, sulfur, chloride and iron. Measurements included shoot fresh and dry weight, stem diameter, shoot length, leaf area, leaf chlorophyll content (SPAD value), presence and concentration of leaf volatiles, fruit cracking, number of fruits, fruit size, nutrient solution uptake, root growth, and substrate pH and electrical conductivity. The crops exhibited varied responses depending on the nutrient treatment applications. In cherry tomatoes, the highest fresh and dry weight were obtained with a 14.28 mM N supply, 50% higher than those grown with 3.57 mM nitrogen. Blueberry plants had a poorer response to the highest nitrogen (21.43 mM), while a combination of 10.71 mM nitrogen and 1.00 - 2.00 mM phosphorus appeared optimal. For kaffir limes, the distribution, presence, and surface percentage of volatiles were influenced by the source and rates of potassium. Raspberry plants developed visual symptoms of interveinal chlorosis, reduced dry biomass, height, and number of leaves when deprived of iron. These investigations provide insights into crop-specific nutrient requirements to optimize growth and yield
Antibiogram profiles of pathogenic and commensal bacteria in goat and sheep feces on smallholder farm
Get PDFIntroduction: The increase of antimicrobial resistance (AMR) in zoonotic pathogens poses a substantial threat to both animal production and human health. Although large-scale animal farms are acknowledged as major reservoirs for AMR, there is a notable knowledge gap concerning AMR in small-scale farms. This study seeks to address this gap by collecting and analyzing 137 fecal samples from goat and sheep farms in Tennessee and Georgia.
Method: Bacteria were identified using culture-dependent methods and polymerase chain reaction (PCR), and antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer Disk Diffusion method.
Results and discussion: The prevalence of E. coli (94.9%) in goats and sheep significantly exceeded (p \u3c 0.05) that of S. aureus (81.0%), Shigella (35.0%), S. saprophyticus, and Salmonella (3.0%). Salmonella occurrence in goat feces (2.2%) was higher than in sheep (0.8%). Notably, 27% of goats and 8% of sheep tested positive for Shigella spp., while 60% of goats and 21% of sheep tested positive for S. aureus. Antibiotic resistance was observed primarily against ampicillin (79.4%), vancomycin (65.1%), and gentamycin (63.6%), significantly surpassing (p \u3c 0.05) resistance to tetracycline (41.6%) and imipenem (21.8%). The penicillin (79.4%), glycopeptide (65.1%), and aminoglycoside (63.6%) antibiotic classes displayed significantly higher (p \u3c 0.05) resistance compared to tetracyclines (45.7%) and carbapenem (21.8%). Our findings suggest that goats and sheep feces may serve as source for multidrug-resistant bacteria, raising concerns about the potential introduction of their fecal matter into soil, water, and eventually to the food chain. This highlights the need for proactive measures to address and mitigate AMR in goats and sheep within small-scale farms
1022181057a
No full texthttps://digitalscholarship.tnstate.edu/m-chamberlain-stones/1038/thumbnail.jp
0709182057b
No full texthttps://digitalscholarship.tnstate.edu/m-chamberlain-landscapes/1009/thumbnail.jp
