University of Belgrade - Faculty of Biology: Open Journal Systems
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DISTRIBUTION AND HABITAT OF CARABUS HUNGARICUS (COLEOPTERA: CARABIDAE) IN SERBIA AND RECOMMENDATION FOR MONITORING
The distribution of Carabus hungaricus in Serbia has not been summarized so far. There are only a few published records of this beetle in Serbia. Due to new findings, it was necessary to update the distribution of this species in Serbia. Recent field research revealed that the largest suitable habitat is the Deliblato Sands in the South Banat District, where this species is common. Other important areas are located near the Romanian border in the vicinity of the city of Vršac and near Mužlja (in the neighborhood of the city of Zrenjanin, Central Banat District), where the northernmost population in Serbia is located. In addition to this overview of distribution, we propose monitoring methodology for C. hungaricus. We believe that regular monitoring can bring the necessary attention to the disappearing steppe habitats and that the results can help to manage them appropriately in the future
GUIDELINES FOR SAMPLING AND PRESERVATION OF BLACK FLIES (DIPTERA: SIMULIIDAE) – AN ATTEMPT TO STANDARDIZE FIELD AND LABORATORY PROCEDURES
This paper provides guidelines for sampling and preserving black flies (Diptera, Simuliidae), aiming to standardize field and laboratory procedures. The authors detail the methods for processing black fly samples, from collection to identification, for each stage. They also propose a sampling protocol that considers various environmental factors influencing species distribution and abundance. The paper is based on the authors' personal experience and aims to contribute to a broader understanding of black fly ecology and taxonomy
SECOND RECORD OF THE ASIAN SPECIES SCOLYTOPLATYPUS TYCON BLANDFORD, 1893 (CURCULIONIDAE: SCOLYTINAE) FOR THE NORTH CAUCASUS
Species of the genus Scolytoplatypus C. F. C. Schaufuss, 1891 are distributed in the Afrotropical and Oriental regions. However, cases of species invasion into other areas are common. After fourteen years on the territory of North Caucasus (Russia: Republic of Adygea), one far-eastern species, Scolytoplatypus tycon Blandford, 1893 (Curculionidae: Scolytinae), was recorded for a second time
NEW DATA ON DISTRIBUTION OF CALIAESCHNA MICROSTIGMA (SCHNEIDER, 1845) (ODONATA: AESHNIDAE) IN SERBIA
Caliaeschna microstigma is a species distributed mainly in the eastern Mediterranean, from Croatia in the west to northern Iran and Caucasus in the east. Until this study, it was reported only from a few localities in Serbia, which are located in far south. Here we have summarized all available data and presented new data on the distribution of C. microstigma in Serbia. The Eastern Spectre was detected at seven new localities (four during field study and three from publicly available databases), extending its current range in Serbia by more than 120 km to the north
NEW DATA ON PERILLUS BIOCULATUS (HETEROPTERA: PENTATOMIDAE) IN SERBIA: DO CLIMATE CHANGE AND A NEW FOOD SOURCE CONTRIBUTE TO THE TRUE BUG EXPANSION?
Perillus bioculatus (Fabricius, 1775) originates from North America, and was introduced to Europe at the beginning of the 20th century. The first findings of the species from nature in Serbia date back to 1996, after which the bug was not registered until 2018. After detecting one individual from 2018, we assumed that Perillus bioculatus would continue to spread throughout Serbia, and recent data have confirmed our suspicions. The paper presents literature and new findings from 2021 and 2022, as well as the conditioning of the spread of the species by the expansion of the range of new prey Ophraella communa LeSage, 1986 - also a non-native insect species in Europe, which was recorded in Serbia for the first time in 2020. In almost all localities from 2022, P. bioculatus was recorded on ragweed together with O. communa
LARVICIDAL EFFECT OF SELECTED SALTS AGAINST THE DENGUE VECTOR MOSQUITO AEDES AEGYPTI (DIPTERA: CULICIDAE) IN BANGLADESH
The container breeding mosquito Aedes aegypti (Linnaeus) is the major universal vector of dengue viruses that cause dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Vector control has an important role in reducing human cases of DHF/DSS. The purpose of this work was to evaluate the larvicidal effect of salt solutions against the larvae of Ae. aegypti. Freshly collected larvae were transferred to the laboratory and reared using rainwater as the rearing medium, with yeast granules as larval food. Five salts, AgNO3, HgCl2, CdCl2, CuSO4, and CuCl2, were tested to assess the larvicidal effect on both the 1st and 3rd instar larvae of Ae. aegypti. Serial concentrations (0, 1, 3, 5, 7 and 10 ppm) of each salt were prepared using distilled water as the solvent. Silver nitrate (AgNO3) was noted as the most effective larvicide, followed by HgCl2, CdCl2, CuSO4, and CuCl2. The LC50 values of AgNO3 against 1st and 3rd instar larvae were 0.118 and 1.659 ppm, and the LC90 values were 2 and 3.347 ppm, respectively. The LT50 values of AgNO3 (1 ppm) against 1st and 3rd instar larvae were 0.575 and 30.42 h, and the LT90 values were 6.00 and 67.49 h, respectively. All 1st instar larvae died, failing to pupate in every salt concentration (1-10 ppm) within 7 days. Third instar larvae were also unable to pupate entirely in AgNO3 and HgCl2 solutions but a very few (5.00-36.66%) pupations were found at 7 ppm, with higher concentrations of CdCl2, CuSo4 and CuCl2 salts. In addition, CdCl2, CuSo4 and CuCl2 prevented 66.66%, 57.14% and 50% of adult emergences from pupae at 5 ppm concentration, respectively. The order of decrease of toxicity for larval mortality was AgNO3>HgCl2>CdCl2>CuSO4>CuCl2, and in preventing pupation and adult emergence it was AgNO3≥HgCl2>CdCl2>CuSO4>CuCl2. AgNO3 was found to have very good potential in the killing of Aedes aegypti larvae, prevention of pupae formation and adult emergence. Therefore, the results obtained could be considered a contribution to the search for eco-friendly larvicides of natural origin. Further studies are needed to understand the residual aquatic toxicity of this salt in the field
THE FIRST RECORD OF THE WEB-SPINNING SAWFLY CAENOLYDA RETICULATA (LINNAEUS, 1758) (HYMENOPTERA: SYMPHYTA: PAMPHILIIDAE) FROM THE BALKANS
During project field activities conducted in May 2021 in the territory of the Landscape of Outstanding Features “Dolina Pčinje” at the locality Vogance, one dead and trampled specimen of the web-spinning sawfly Caenolyda reticulata (Linnaeus, 1758) was found. The new finding is the first report of the species from Serbia, as well as from the Balkans, and significantly expands its known distribution into southeast Europe. The host plants of this species are pines (Pinus spp.), of which black pine (Pinus nigra subsp. nigra) occurs in the Pčinja River valley
PERIPHYLLUS CALIFORNIENSIS (SHINJI, 1917) AND TINOCALLIS SALTANS (NEVSKY, 1929) (HEMIPTERA: APHIDIDAE), TWO ALIEN APHID SPECIES NEW TO THE FAUNA OF SERBIA
This paper presents two alien species of aphids new to the fauna of Serbia with details on their morphology and biology, with original photographs. Periphyllus californiensis (Shinji, 1917) is an invasive species that belongs to the subfamily Chaitophorinae and was found for the first time in 2021 on Acer palmatum Thunb. Tinocallis saltans (Nevsky, 1929) belongs to the subfamily Calaphidinae and was found on Ulmus pumila L. in 2020 and 2021. Both species were registered in Belgrade. There is now a total of 393 registered species of aphids in Serbia
THE SUBLETHAL EFFECTS OF FIVE COMMERCIAL INSECTICIDES ON THE AMYLOLYTIC AND PROTEOLYTIC ACTIVITY OF THE BIOCONTROL AGENT, HABROBRACON HEBETOR SAY (HYMENOPTERA: BRACONIDAE)
Habrobracon hebetor Say is an important biological control agent that has a wide range of lepidopteran hosts, especially from the Noctuidae and Pyralidae families. Nutrition is a very important element in the efficiency of natural enemies in integrated pest management (IPM). In the presented research, the sublethal effects of five selected chemical and botanical insecticides, fenvalerate, propargite, buprofezin, dayabon and palizin, on the activity of amylase and protease enzymes in the midgut of this important ectoparasitoid wasp were studied under laboratory conditions. Changes in the activity of enzymes were investigated by LC30 treatment of the female wasps, which were 0.14, 7.01, 3.04, 9.58 and 2.53 mg a.i./mL, respectively. The obtained results showed that this parasitoid wasp has a long midgut in its digestive tract. Also, there were significant differences among the enzymatic treatments (F5,12 =14.695 and 99.278) except protein concentration (F5,12 = 0.670). Regarding the amylase enzyme, the highest and lowest activities were obtained in the control and fenvalerate treatment (0.0435±0.0023 mU/mg vs. 0.0277±0.0010 mU/mg). In addition, the highest proteolytic activity was observed in the control (4.9817±0.0268 U/mg) and the lowest in the propargite treatment (3.2231±0.0917 U/mg). By closely investigating the results, dayabon and palizin showed the lowest negative effects on the enzymes and could be applied together with this parasitoid wasp in IPM designs
AN OVERVIEW ON THE SUBFAMILY CREMASTINAE FÖRSTER, 1869 (HYMENOPTERA: ICHNEUMONIDAE) FROM TURKEY
Fauna of the subfamily Cremastinae Förster, 1869 (Ichneumonidae: Hymenoptera) on the territory of Turkey was investigated. The survey was conducted during the period 1995-2020. A total of 54 species belonging to eight genera have been listed. Among the material collected in the past 25 years, the species Cremastus brevicornis Kolarov & Beyarslan, 1999; C. kasparyani Kolarov Gürbüz & Birol, 2014; C. petiolaris Kolarov & Beyarslan; 1999; C. tristator Aubert, 1970; Nothocremastus beyarslani Kolarov, 1997 and Temelucha turcata Kolarov & Beyarslan, 1999 were listed as endemic to the investigated teritorry. Additionally, a male of Eucreamastus priebei Kolarov, 1999 is recorded for the first time from Turkey. Turkish species of the subfamily Cremastinae have been analyzed according to biogeographical and zoоgeographical features