Soka University Repository / 創価大学機関リポジトリ
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A preliminary study on the Guan-yin yi-shu : Focusing on characteristics of the citations
Get PDF『観音義疏』に引用される中国で成立した他の注釈書、仏教文献をとりあげ、智顗（天台家）独自の観音品解釈や、他の注釈書からの影響を明らかにすることを目的として論じた。『観音義疏』に出る引用について、『妙法蓮華経文句』（以下『法華文句』）釈観音品、『法華経義記』（以下『法華義記』）、『法華義疏』と比較した結果、『観音義疏』が『法華義疏』、もしくは『法華義疏』が参照した他の著作を参照し、編集した可能性が考えられる。ただし、『観音義疏』の著者が智顗ではない第三者であるとしても、ほぼ全てが吉蔵を参照した内容なのではなく、天台家独自の解釈も表れていると筆者は考える。また、『観音義疏』の内容が智顗の初期の解釈で、後に『法華文句』の解釈へと変遷していったと考えられる箇所も明らかにした。departmental bulletin pape
Evaluation Tendency of Experienced Child Care Providers on the Appropriateness of Manner of Speaking by Current Child Care Providers and Students to 3-Year-Old Children —Focusing on Reflection, Evaluation, Interpretation, and Meaning Making—
Get PDFThe purpose of this study was to clarify the evaluation tendency of experienced caregivers on the appropriateness of manner of speaking by current caregivers and students, who were interacting with 3 -year-old children with "desirable attitude" and "attitude needs consideration".
We analyzed evaluations by a former public kindergarten director who had worked for 36 years and a child care provider who had worked at a private kindergarten for 15 years and had no experience in a supervisory position. In both evaluations, a similar trend was found for the "attitude needs consideration". However, there was a discrepancy between the two in terms of the “desirable attitude”. This result suggests that reflection, evaluation criteria, interpretation, and meaning making based on childcare experience are more important in capturing "manner of speaking" for "attitude needs consideration”.departmental bulletin pape
Global Citizenship Education: Teaching Strategies for a Gendered Perspective
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Studies on the iron oxidation/mineralization of ferritin
Get PDF創価大学博士（理学）Ferritin keeps cells from being damaged by reactive oxygen species produced by Fe2+ and storages the oxidized iron in its cavity as iron cores. The iron core contains a various amount of inorganic phosphate (Pi). In bacteria, the amount of phosphate is comparable to that of iron. In contrast, ferritins from mammals contain only a tenth phosphate. It is not clear how the phosphate works in the iron oxidation and mineralization process. In this research, I investigated the effect of the phosphate on the structural feature of iron cores formed in the cavity of Escherichia coli ferritin (EcFtnA) and on its function. First, the structure of iron cores was characterized using analytical ultracentrifugation, transmission electron microscopy and small-angle X-ray scattering. These experiments revealed that the iron core was a cluster of small particles of which diameter were 2-4 nm in the absence of phosphate whereas it had a hollow spherical structure of which the inner and outer diameters were 6 and 8 nm, respectively. The effects of phosphate on the oxidation/mineralization kinetics were also investigated spectrophotometrically. The oxidation reaction showed at least two kinetic phases when iron atoms beyond 72 Fe/protein shell were added. The initial phase corresponds to the binding of three irons to a ferroxidase center and subsequent oxidation, whereas the second one reflects the turnover, that is, the movement of oxidized iron from the ferroxidase center to the cavity. By comparing the kinetic curves with and without phosphates, it was revealed that the phosphate accelerated only the second phase, suggesting that the phosphate increases the turnover rate of the iron oxidation and/or mineralization process by EcFtnA.
Neuroferritinopathy is a rare disease caused by mutations to human ferritin light chain gene (ftl1). While 9 of ten mutations reported to date produce ferritin mutants extended from 4 to 16 amino acid residues in the C-terminal region, A96T is a point mutant associated with neuroferritinopathy. The functional information of A96T has not been obtained in contrast to frameshift mutants. The iron incorporation activity of A96T mutant was investigated by native-PAGE using Prussian blue staining and change in absorbance intensity at 310nm. The result revealed that the iron incorporation activity of A96T is similar to that of human ferritin light chain wild type, and suggested that the pathogenic mechanism caused by A96T is different from the model proposed for frameshift mutants.doctoral thesi