Journal Widya Mandala Catholic University Surabaya / Jurnal Universitas Katolik Widya Mandala Surabaya
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Perbandingan Kadar Flavonoid Total Ekstrak Etanol 96% dan Ekstrak Etil Asetat Daun Johar (Cassia siamea L.)Secara Spektrofotometri UV-Vis
Get PDFJohar (Cassia siamea L.) is a plant that is used in traditional medicine. Phytochemical test results of johar leaves (Cassia siamea L.) using 96% ethanol and ethyl acetate solvents showed the presence of flavonoid compounds. The flavonoid content that functions as an antioxidant in johar leaves can be used as a traditional medicine. The content of extracted compounds is influenced by the solvent's polarity. This study aims to determine the comparison of total flavonoid content of 96% ethanol extract and ethyl acetate extract in johar leaves (Cassia siamea L.). This research is an experimental research conducted by extraction method using 96% ethanol and ethyl acetate solvents. Qualitative analysis with color reaction test while quantitative analysis using UV-Vis spectrophotometry at a maximum wavelength of 441.6 nm. The standard standard solution used was quercetin. The results showed the average flavonoid content in 96% ethanol extract was 25.5832 ± 0.1515 %b/b and ethyl acetate extract was 70.9186 ± 0.1567 %b/b. The data was tested by Independent Sample T-Test which showed a significance value of 0.000 (sig <0.05), meaning that there is a significant difference between 96% ethanol solvent and ethyl acetate solvent. The conclusion of this study is the determination of flavonoid levels of johar leaf extract using ethyl acetate solvent produces greater flavonoid levels compared to 96% ethanol solvent.Johar (Cassia siamea L.) adalah tanaman yang digunakan dalam pengobatan tradisional. Hasil uji fitokimia daun johar (Cassia siamea L.) menggunakan pelarut etanol 96% dan asetat etil menunjukkan adanya senyawa flavonoid. Kandungan flavonoid yang berfungsi sebagai antioksidan dalam daun johar dapat digunakan sebagai obat tradisional. Kandungan senyawa yang diekstraksi dipengaruhi oleh polaritas pelarut. Penelitian ini bertujuan untuk menentukan perbandingan kandungan flavonoid total antara ekstrak etanol 96% dan ekstrak etil asetat pada daun johar (Cassia siamea L.). Penelitian ini merupakan penelitian eksperimental yang dilakukan dengan metode ekstraksi menggunakan pelarut etanol 96% dan etil asetat. Analisis kualitatif dilakukan dengan uji reaksi warna, sedangkan analisis kuantitatif menggunakan spektrofotometri UV-Vis pada panjang gelombang maksimum 441,6 nm. Larutan standar yang digunakan adalah quercetin. Hasil menunjukkan bahwa kandungan flavonoid rata-rata pada ekstrak etanol 96% adalah 25,5832 ± 0,1515 %b/b dan ekstrak etil asetat adalah 70,9186 ± 0,1567 %b/b. Data diuji dengan Uji Independen Sampel T-Tes yang menunjukkan nilai signifikansi 0,000 (sig <0,05), artinya terdapat perbedaan yang signifikan antara pelarut etanol 96% dan pelarut etil asetat. Kesimpulan dari penelitian ini adalah penetapan kadar flavonoid ekstrak daun johar menggunakan pelarut etil asetat menghasilkan kadar flavonoid yang lebih tinggi dibandingkan dengan pelarut etanol 96%
Penetapan Kadar Flavonoid Pada Daun Matoa (Pometia pinnata) Dengan Perbandingan Metode Ekstraksi Menggunakan Metode Spektrofotometri UV-Vis
Get PDFMatoa plant (Pometia pinnata) is a typical plant from Indonesia, especially in Papua Province, which contains active compounds, one of which is flavonoids in its leaves. The benefits of flavonoids in matoa leaves (Pometia pinnata) are obtained by extraction of dry simplicia of matoa leaves (Pometia pinnata) with 96% ethanol solvent using the maceration and soxhlet extraction methods. After that, the flavonoids in matoa leaves were measured using a UV-Vis spectrophotometer at the maximum wavelength. This study aims to compare the flavonoid levels of matoa leaves extract (Pometia pinnata) with different extraction methods, namely the maceration and soxhlet extraction methods. The principle of the maceration method is to soak 250 g of simplicia powder with 750 ml of 96% ethanol solvent in a tightly closed vessel and leave for 3 days at room temperature, when the powder and solvent reach equilibrium, the extraction is stopped and remaceration is carried out 5 times. Meanwhile, for soxhletation, the extraction was carried out with 30 g of simplicia powder and flowed with 300 ml of 96% ethanol solvent continuously, the extraction was stopped when the color of the sample immersed in the solvent changed to clear. The flavonoid content of matoa leaves (Pometia pinnata) was determined by UV-Vis spectrophotometry which measured the absorbance at a maximum wavelength of 441.6 nm and replicated 3 times. The flavonoid content obtained in the maceration and soxhlet extraction were 22,8006 ± 0,0915 % b/b and 25,5141 ± 0,0625 % b/b. The statistical test showed that there was significant difference between flavonoid content from maceration and soxhlet extraction methods.Tanaman matoa (Pometia pinnata) merupakan tanaman khas dari Indonesia khususnya Provinsi Papua yang memiliki kandungan senyawa aktif salah satunya adalah flavonoid pada daunnya. Manfaat flavonoid pada daun matoa (Pometia pinnata) diperoleh dengan cara mengekstrak simplisia kering daun matoa (Pometia pinnata) dengan pelarut etanol 96% menggunakan metode ekstraksi maserasi dan soxhlet. Setelah itu, flavonoid pada daun matoa diukur kadarnya menggunakan spektrofotometer UV-Vis pada panjang gelombang maksimum. Penelitian ini bertujuan untuk membandingkan kadar flavonoid ekstrak daun matoa (Pometia pinnata) dengan metode ekstraksi yang berbeda yaitu metode ekstraksi maserasi dan soxhlet. Prinsip metode maserasi yaitu merendam serbuk simplisia sebanyak 250 g dengan pelarut etanol 96% sebanyak 750 ml dalam bejana tertutup rapat dan dibiarkan selama 3 hari pada suhu kamar, apabila serbuk dan pelarut telah mencapai kesetimbangan maka ekstraksi dihentikan dan dilakukan maserasi ulang sebanyak 5 kali. Sedangkan untuk soxhletasi dilakukan ekstraksi dengan serbuk simplisia sebanyak 30 g dan dialiri dengan 300 ml pelarut etanol 96% secara terus menerus, ekstraksi dihentikan apabila warna sampel yang tercelup dalam pelarut berubah menjadi bening. Kadar flavonoid daun matoa (Pometia pinnata) ditetapkan dengan spektrofotometri UV-Vis yang diukur serapannya pada panjang gelombang maksimum 441,6 nm dan diulang sebanyak 3 kali. Kadar flavonoid yang diperoleh pada ekstraksi maserasi dan soxhlet masing-masing sebesar 22,8006 ± 0,0915 % b/b dan 25,5141 ± 0,0625 % b/b. Uji statistik menunjukkan bahwa terdapat perbedaan yang nyata antara kadar flavonoid hasil ekstraksi metode maserasi dan soxhlet
Pengaruh Literasi Digital terhadap Literasi Kesehatan Digital Tenaga Kesehatan di Indonesia
Get PDFThe advancement of information and communication technology requires healthcare professionals to have strong digital competencies in consuming, acting upon, and producing digital health information. In Indonesia, some social media content created by healthcare professionals has been deemed unethical or unwise, highlighting the importance of digital literacy in this sector. Although studies on digital literacy and digital health literacy exist, research specifically focusing on Indonesian healthcare professionals is still limited. This study aims to analyze the influence of digital literacy on digital health literacy among healthcare professionals. Using a survey method, an online questionnaire was distributed to 387 respondents. The findings indicate that respondents’ levels of digital literacy and digital health literacy are moderate. While respondents generally perceive themselves as proficient in technical digital skills, they report lower confidence in broader digital competencies such as sharing opinions, participating in online activities, and protecting themselves from internet-related risks. Although they demonstrate stronger operational skills, they encounter challenges in navigating digital health information, evaluating the reliability of information, safeguarding personal privacy, and adding self-generated content. These results confirm that digital literacy significantly influences digital health literacy, emphasizing the need for healthcare professionals to develop comprehensive digital skills. Strong digital capabilities are essential for engaging effectively with digital health information and establishing credibility as sources on social media. The study underscores the urgent need to enhance digital capacity, particularly in device security, information navigation, content creation, digital participation, and protection from privacy breaches and other online risks.
ABSTRAK Perkembangan teknologi informasi dan komunikasi menuntut tenaga kesehatan untuk memiliki kompetensi digital dan keterampilan dalam mengonsumsi, mengambil tindakan atas, serta memproduksi informasi kesehatan digital. Beberapa tahun terakhir, terdapat sejumlah konten media sosial oleh tenaga kesehatan di Indonesia yang dinilai kurang etis dan bijak. Sejumlah studi mengenai literasi digital dan literasi kesehatan digital telah dilakukan. Namun, di Indonesia masih terbatas studi mengenai literasi digital dan literasi kesehatan digital tenaga kesehatan. Riset ini ditujukan untuk menguji dan menganalisis pengaruh literasi digital terhadap literasi kesehatan digital pada tenaga kesehatan. Metode survei dilakukan melalui kuesioner daring kepada 387 tenaga kesehatan. Hasil riset menunjukkan bahwa secara umum, literasi digital dan literasi kesehatan digital responden berada pada tingkat moderat. Responden cenderung menilai diri mereka lebih memiliki keterampilan teknis digital dibandingkan kompetensi digital yang mencakup berbagi opini, berpartisipasi, dan perlindungan dari risiko penggunaan internet. Responden lebih unggul dalam keterampilan operasional, namun menghadapi tantangan dalam menavigasi, mengevaluasi reliabilitas, melindungi privasi, dan menambahkan konten yang telah diproduksi sendiri. Temuan ini menegaskan bahwa literasi digital memiliki pengaruh signifikan terhadap literasi kesehatan digital, menunjukkan bahwa kemampuan digital yang baik menjadi elemen penting bagi tenaga kesehatan untuk mendukung keterlibatan mereka memanfaatkan internet dalam konteks kesehatan serta partisipasi aktif sebagai sumber informasi di media sosial. Studi ini memperkuat urgensi peningkatan kapasitas digital terutama terkait keamanan perangkat digital, navigasi dan produksi pesan, partisipasi dalam aktivitas digital, dan perlindungan privasi serta risiko lain dari penggunaan internet
Pengaruh Kompetensi Komunikasi Hati dan Dukungan Sosial Teman Sebaya Terhadap Resiliensi Akademik Mahasiswa Tingkat Akhir
Get PDFFinal-year students often face complex academic pressures. Academic resilience is a key competency for students to be able to face pressure. Academic resilience is influenced by internal factors such as heart communication competency and external factors such as peer social support. The study aims to analyze the effect of heart communication competency (X1) and social support (X2) on the academic resilience of final-year students (Y). The study used a quantitative method. The research sample was 80 final-year students of the Faculty of Vocational Studies, UNY. Data collection through surveys to samples. Data analysis with parametric statistics in the form of linearity tests and hypothesis tests through simple correlation analysis methods, multiple correlation analysis, and multiple regression analysis. The results of the study showed that there was a positive and significant partial influence between X1 and Y of 40.2%, there was a positive and significant influence between X2 and Y of 26.5%, and there was a positive and significant influence together between heart communication competency and peer social support of 30.2% on academic resilience in students of the Faculty of Vocational Studies, UNY. The findings show that the competence of heart communication, with the dimensions of thinking, feeling, managing heart waste, sympathy, empathy, peace, and happiness, plays a dominant role in the academic resilience of final-year students. The contribution of the research provides a new understanding of the importance of heart communication competence and peer social support in supporting the academic resilience of final-year students. The results of the study support the Theory of Heart Communication, which emphasizes that thinking, feeling, managing heart waste, sympathy, and empathy for a peaceful and happy life can positively increase the academic resilience of final-year students. The study successfully tested the relevance of the Theory of Heart Communication to the academic resilience of final-year students at the Faculty of Vocational Studies, UNY, as a novelty.
ABSTRAK Mahasiswa tingkat akhir kerap menghadapi tekanan akademik yang kompleks. Resiliensi akademik menjadi kompetensi kunci agar mahasiswa mampu menghadapi tekanan. Resiliensi akademik dipengaruhi faktor internal seperti kompetensi komunikasi hati dan eksternal seperti dukungan sosial teman sebaya. Penelitian bertujuan untuk menganalisis pengaruh kompetensi komunikasi hati (X1) dan dukungan sosial (X2) terhadap resiliensi akademik mahasiswa tingkat akhir (Y). Penelitian menggunakan metode kuantitaif. Sampel penelitian sebanyak 80 mahasiswa tingkat akhir Fakultas Vokasi UNY. Pengumpulan data melalui survei kepada sampel. Analisis data dengan statistik parametris berupa uji linearitas dan uji hipotesis melalui metode analisis korelasi sederhana, analisis korelasi berganda dan analisis regresi berganda. Hasil penelitian menunjukkan terdapat pengaruh positif dan signifikan secara parsial antara X1 dengan Y sebesar 40,2%, terdapat pengaruh positif dan signifikan antara X2 dengan Y sebesar 26,5%, dan terdapat pengaruh positif dan signifikan secara bersama-sama antara kompetensi komunikasi hati dan dukungan sosial teman sebaya sebesar 30,2% terhadap resiliensi akademik pada mahasiswa Fakultas Vokasi UNY. Temuan menunjukkan kompetensi komunikasi hati, dengan dimensi olah pikir, olah rasa, kelola sampah hati, simpati, empati, damai, dan bahagia berperan dominan terhadap resiliensi akademik mahasiswa tingkat akhir. Kontribusi penelitian memberikan pemahaman baru pentingnya kompetensi komunikasi hati dan dukungan sosial teman sebaya dalam mendukung resiliensi akademik mahasiswa tingkat akhir. Hasil penelitian mendukung Teori Komunikasi Hati yang menekankan bahwa olah pikir olah rasa, kelola sampah hati, simpati, empati, untuk hidup damai dan bahagia secara positif dapat meningkatkan resiliensi akademik mahasiswa tingkat akhir. Penelitian berhasil menguji relevansi Teori Komunikasi Hati terhadap resiliensi akademik mahasiswa tingakt akhir di Fakultas Vokasi UNY sebagai novelty
THE EFFECT OF ROSEMARY ESSENTIAL OIL (ROSMARINUS OFFICINALIS L.) ON IMPROVING SHORT-TERM MEMORY IN ELDERLY PEOPLE
Get PDFMemory is the human ability to receive and store information. With increasing age, the brain's work function will also experience a decline, which will have an impact on individual cognitive function. Memory decline is a common thing that occurs in people of older age. Rosemary essential oil is one of the additional therapies that are useful in improving working memory and short-term memory. This study aims to determine the effect of rosemary essential oil on short-term memory in elderly people. This research is quasi-experimental with group control, with data collection techniques, namely, random sampling. The research was measured using the digit span test as a short-term memory test involving 32 elderly respondents with an age range of 65-85 years. This research was conducted by administering rosemary essential oil to the treatment group and jojoba oil to the control group by inhalation using a mask for 7 days in the morning, with the results of the pretest and posttest comparison between the two groups then analyzed using the Wilcoxon test. The results obtained from the study were that there was a significant value in the experiment group given rosemary essential oil, namely with a p-value of (p<0.005), which means that there is an influence between rosemary essential oil and short-term memory
HOW PRO-INFLAMMATORY WESTERN DIETS AND ANTI-INFLAMMATORY DIETARY INTERVENTIONS INFLUENCE NONALCOHOLIC FATTY LIVER DISEASE (NAFLD) PROGRESSION: A NARRATIVE REVIEW OF NUTRITIONAL MECHANISMS OF ACTION
Get PDFUnhealthy diets, such as Western-type diets, are major contributors to metainflammation underlying the progression of nonalcoholic fatty liver disease (NAFLD). This narrative review analyzed 10 studies (2014–2024) from Google Scholar and PubMed to explore dietary roles in NAFLD progression. Western-type, hypercaloric, high-fructose, and HFHS diets worsen NAFLD by increasing inflammatory biomarkers and reducing oxidative stress. Conversely, dietary interventions, including the DASH diet, freshwater fish–based diets, and the Mediterranean diet, attenuate metainflammation and slow NAFLD progression. The significance of this topic lies in the global health burden of NAFLD and the increasing recognition of metainflammation as a key driver of its progression. While several pharmacological candidates have shown promise, dietary strategies remain essential in clinical practice due to their accessibility, safety, and preventive potential. This review underscores the clinical relevance of targeting metainflammation through specific dietary interventions and offers insights for both therapeutic guidance and public health policy
CAN AUTISM BE DETECTED EARLY IN LIFE?
Get PDFAutism Spectrum Disorder (ASD) is a neurodevelopmental condition characterized by challenges in social communication and repetitive behaviors, typically emerging in early childhood. Early detection of ASD is critical, as timely intervention can significantly improve long-term outcomes in affected individuals. This review explores the possibility of detecting autism early in life, focusing on early behavioral signs, such as reduced eye contact and delayed social communication, which may be observable within the first year of life. The efficacy of the Modified Checklist for Autism in Toddlers Revised/Follow (M-CHAT-R/F), Autism Diagnostic Observation Schedule in Autism Screening (ADOS), and the availability of emerging technologies like eye-tracking and neuroimaging that may offer more precise early detection methods is discussed. Furthermore, the role of genetic and environmental factors in the onset of ASD is examined, highlighting how early identification of at-risk populations could be facilitated through genetic screening. Finally, this review underscores the importance of early intervention programs, which have been shown to enhance developmental trajectories when initiated before three years of age. Ongoing research into technological advancements and predictive models promises to refine early detection methods, enabling better access to screening and care
THE IMMUNOMODULATORY ROLE OF INULIN AND GLUCOMANNAN IN OBESITY-RELATED SYSTEMIC INFLAMMATION: CD3CD4 ANALYSIS IN HFHS-FED C57BL/6 MICE
Get PDFBackground: Obesity is a global health burden and one of the risk factor for non- communicable disease such as cardiovascular disease and type 2 diabetes mellitus. Western diet characterized by consumption of high-fat high-sucrose (HFHS) leads to gut microbiota dysbiosis and metabolic endotoxemia, which can cause chronic inflammation. This condition can affect the immune systems, including an increase of CD3+CD4+ splenocyte populations. The consumption of dietary fibers such as inulin and glucomannan, which has prebiotic function, is considered to play a role as antiinflammatory and antiobesity agents.
Objective: This research aims to investigate the effects of inulin, glucomannan, and combination of inulin-glucomannan on the CD3+CD4+ splenocyte populations in HFHS diet- induced obese mice.
Methods: This research uses a true experimental with a post-test only control group design. A total of 20 male C57BL/6 aged 6 weeks mice were randomized into five groups consist of standard diet (AQ), HFHS diet (KN), HFHS diet with inulin (IO), HFHS diet with glucomannan (GO), HFHS diet with combination of inulin-glucomannan (IG) for 56 days. Body weight was measured weekly, and CD3+CD4+ splenocyte populations was measured on the final day of intervention using the FACS method.
Results: This research proves HFHS diet increased both body weight and CD3+CD4+ splenocyte populations. All fiber treatments significantly prevented weight gain. Glucomannan significantly reduced the CD3+CD4+ splenocyte populations in obese mouse model. However, the combination of inulin and glucomannan did not exhibit a synergistic effect in reducing the CD3+CD4+ splenocyte populations.
Conclusion: Inulin, glucomannan, and the combination of inulin-glucomannan prevented weight gain and reduced the CD3+CD4+ splenocyte populations in the HFHS diet-induced obese mice
EFFECT OF EMPLOYEE ENGAGEMENT ON MARKETING STRATEGY IN SERVICE FIRMS IN OGUN STATE NIGERIA
Get PDFThis paper examined the effect of employee engagement on marketing strategy amongst service firms in Ogun State, Nigeria. The targeted population for the study consists of marketing managers, HR heads, and senior managers. Building on Khan's (1990) theory of employee engagement, three dimensions - employee empowerment [Emp_E], employee advocacy [Emp_A], and employee knowledge [Emp_K] - were used to profile employee engagement. Cochran's (1997) finite sample size determination was adopted. Using a simple random technique, electronic closed-ended questionnaires were administered to solicit responses from 333 respondents. The line of best fit model was analyzed with standardized multiple regression analysis (SMRA). Findings revealed that the predictor constructs (Emp_E, Emp_A, and Emp_K) explained 72.9% of the outcome construct (Mkt_S) such that adjusted R2 = .729, F(3, 301) = 273.901, p < 0.001. However, an independent analysis of the predictor constructs revealed that only Emp_E (β1= .613, p <0.001) and Emp_K (β3= .432, p <0.001) have a significant positive relationship with Mkt_S. Evidence shows that Emp_A (β2 = 0.089, p = 0.079) has a positive relationship, but it does not make a statistically significant contribution to the marketing strategy. This insignificant contribution may be due to existing literature which argues that Emp_A is a form of organizational citizenship behaviour which is not directly called on or explicitly rewarded by organizations. Overall, since the regression line is statistically significant, this study submits that there is a significant positive relationship between employee engagement and marketing strategy. This study contributes in two ways. First, it expands Kahn’s theory of employee engagement. Second, it informs marketing managers / HR heads on the importance of designing an inclusive employee growth-oriented program that will facilitate the successful implementation of the marketing strategy. The study recommends that service firms need a strong understanding of key drivers or underlying mechanisms that can facilitate advocacy behaviours in employees. This study used a cross-sectional data approach, which may have biased the results. Hence, the identification of future areas of research has been highlighted
A Critical Discourse Analysis of Women Leadership within Feminism in the "Cruella" Movie
No full textThe research discusses women's leadership within feminism through the three-dimensional framework of Fairclough's Critical Discourse Analysis (CDA). This study uses a qualitative descriptive research method that analyzes utterances in dialogues or monologues, and movie scenes related to women leadership. From the analysis by using the textual practice, it is concluded that the two characters of female leaders, Cruella and Baroness both have leadership traits that are reflected in their dialogue. The dialogue is categorized into types of sentences, namely descriptive, interrogative, imperative, and exclamative sentences, along with the use of pronouns and modality. The discursive practice influences the production process so it affects the text production and characterization of the characters in their positions as leaders. In addition, sociocultural practice shows how women leadership in the movie is influenced by real social phenomena related to women in the work field at that time and shows how women take masculine behavior to suit the work environment, like confidence, ambition, and competitiveness. Furthermore, by highlighting the assertive leadership traits of these characters, this movie provides a nuance of opposing the patriarchal system, thus showing how women are able to become leaders and succeed in breaking down societal norms