JKI Open Journal Systems (Julius Kühn-Institut)

Redaktion, Die

Termine und Veranstaltungen
Journal für Kulturpflanzen 71. 2019
226
Term
ine und Veranstaltungen
Datum/Ort Thema Veranstalter/Kontakt
13. – 17.07.2019
San Jose, 
California, USA
Plant Biology 2019 
– ASPB Annual Plant Biology Meeting
https://aspb.org/meetings-events/
plant-biology-annual-meeting/ 
15. – 16.08.2019
Braunschweig
IX. UPC – Wie funktioniert Stadtgrün? 
Interdependenz vielschichtiger 
Wechselwirkungen im urbanen Grün: 
„Beziehungskisten“
https://plant-protection.net/de/upc/ 
Veranstalter: DPG, JKI, DASoL, BEUTH, LKSH 
Kontakt: Falko Feldmann 
E-Mail: : feldmann@phytomedizin.org
29. – 31.08.2019
Raitenhaslach
63. Jahrestagung 
der Arbeitsgemeinschaft Grünland und 
Futterbau der Gesellschaft für 
Pflanzenbauwissenschaften e.V.
https://www.gpw.uni-kiel.de/de/ag/
aggf/aggf2019/tagungsvorwort 
Veranstalter: Gesellschaft für 
Pflanzenbauwissenschaften
04. – 08.09.2019
Braunschweig
Symposium: Impact of Anticipating 
Research in the Past on Research 
in the Future
https://www.julius-kuehn.de/
veranstaltungen/veranstaltung/news/
symposium-impact-of-anticipating-
research-in-the-past-on-research-
in-the-future/ 
Veranstalter: JKI, CAS, GELIFES, IAE/CAS 
Kontakt: Silvia Haneklaus 
E-Mail: silvia.haneklaus@julius-kuehn.de
05. – 06.09.2019
Jena
40. Tagung 
des Arbeitskreises Phytobakteriologie
https://plant-protection.net/de/
arbeitskreise/phytobakteriologie/ 
Veranstalter: DPG 
Kontakt: Jan Nechwatal 
E-Mail: jan.nechwatal@LfL.bayern.de
10. – 12.09.2019
Berlin
Jahrestagung 2019 
– 62. Tagung der Gesellschaft für 
Pflanzenbauwissenschaften e. V. 
„Landscape matters – Agrarland-
schaften der Zukunft“
https://www.gpw.uni-kiel.de/de/
jahrestagung/jahrestagung/
jahrestagung-2019 
Veranstalter: Gesellschaft für 
Pflanzenbauwissenschaften 
Kontakt: Claas Nendel (ZALF) 
E-Mail: nendel@zalf.de
13. – 15.09.2019
Lichtenau
DLG Waldtage 
Forstpraxis live
https://www.dlg-waldtage.de/start/ 
Veranstalter: DLG,  Landesbetrieb Wald 
und Holz Nordrhein-Westfalen, Wald-
bauernverband NRW e.V., 
Briloner Stadtforst 
Kontakt: Jan Kath 
E-Mail: Waldtage@DLG.org
19. – 20.09.2019
Kassel
44. Jahrestagung 
des DPG-Arbeitskreises Phytomedizin 
in den Tropen und Subtropen
https://plant-protection.net/de/
arbeitskreise/tropen-subtropen/ 
Veranstalter: DPG 
Kontakt: Stephan Winter, Falko Feldmann 
E-Mail: stephan.winter@dsmz.de, 
falko.feldmann@julius-kuehn.de
26. – 27.09.2019
Dresden
2019 meeting of the GPZ working group 
Cytogenetics 
„Chromosome biology, genome 
evolution and modern cytogenetics 
in the context of plant breeding“
https://tu-dresden.de/mn/biologie/
botanik/molbio/gpz2019#intro-target-box 
Veranstalter: TU Dresden
07. – 09.10.2019
Freising
XXIVth EUCARPIA Maize and Sorghum 
Conference 
Integrating quantitative and molecular 
genetics to develop new breeding 
methods in maize and sorghum
http://www.plantbreeding.wzw.tum.de/
index.php?id=84 
Kontakt: Chris-Carolin Schön 
E-Mail: plantbreeding.wzw@tum.de
Termine und Veranstaltungen
Journal für Kulturpflanzen 71. 2019
227
Term
ine und Veranstaltungen
12.10.2019
Dresden
15. Pillnitzer Apfeltag https://www.julius-kuehn.de/
veranstaltungen/veranstaltung/news/
15-pillnitzer-apfeltag/ 
Veranstalter: JKI 
Kontakt am JKI: 
Stefanie Hahn/Pressestelle und 
Andreas Peil/Institut ZO
10. – 14.11.2019
Hyderabad, India
XIX International Plant Protection 
Congress – IPPC 2019
https://www.plantprotection.org
10. – 16.11.2019
Hannover
Agritechnica – The World’s No. 1 https://www.agritechnica.com/de/
12. – 14.11.2019
Dubai
Ipm Dubai und Wop Dubai https://www.ipm-essen.de
13. – 14.11.2019
Quedlinburg
2. Treffen der Partner der DGO https://www.julius-kuehn.de/
veranstaltungen/veranstaltung/news/
2-treffen-der-partner-der-dgo/ 
Kontakt: Monika Höfer
19. – 20.11.2019
Berlin
Konferenz: „Genetic diversity 
– The key for improving drought 
stress tolerance in crops“
https://conference.geneticdiversity.
julius-kuehn.de/ 
Veranstalter: BMEL, JKI 
E-Mail: florian.bittner@julius-kuehn.de
20. – 21.11.2019
Göttingen
Wintertagung 2019 
der GPZ Arbeitsgemeinschaft 
für Kartoffelzüchtung und 
Pflanzgutproduktion (AG 13)
https://gpz-online.de/terminkalender/ 
Kontakt: Bernd Truberg 
E-Mail: truberg@norika.de
25. – 26.11.2019
Köln
20. Jahrestagung 
des DPG-Arbeitskreises Vorratsschutz
https://plant-protection.net/de/
arbeitskreise/vorratsschutz/ 
Kontakt: Cornel Adler, Jürgen Böye 
E-Mail: cornel.adler@julius-kuehn.de, 
jb-consulting@boeye.de
26. – 27.11.2019
Köln
22. Tagung 
des DPG-Arbeitskreises Wirbeltiere
https://plant-protection.net/de/
arbeitskreise/wirbeltiere/ 
Kontakt: Jens Jacob, Stefan Endepols 
E-Mail: jens.jacob@julius-kuehn.de, 
stefan.endepols@bayer.com
26. - 27.11.2019
Karlsruhe
37. Tagung des DPG-Arbeitskreises 
Nutzarthropoden und Entomopatho-
gene Nematoden
https://plant-protection.net/de/
arbeitskreise/nutzarthropoden/
Veranstalter: JKI, e-nema
Kontakt: Annette Herz, Ralf-Udo Ehlers
E-Mail: annette.herz@julius-kuehn.de; 
ehlers@e-nema.de
04.12.2019
Berlin
DLG-Kolloquium 2018 
Landwirtschaft im Klimawandel 
– Strategien für Ackerbau und 
Tierhaltung
https://www.dlg.org/de/landwirtschaft/
veranstaltungen/dlg-kolloquium/ 
E-Mail: M.Packeiser@DLG.org
09. – 10.12.2019
Fulda
35. Resistenztagung 
Vortragstagung „Fortschritte in 
der Krankheitsbekämpfung und 
Resistenzzüchtung bei landwirtschaft-
lichen Kulturpflanzen“
https://gpz-online.de/terminkalender/ 
Kontakt: Prof. Dr. Thomas Miedaner 
E-Mail: 
thomas.miedaner@uni-hohenheim.de
Datum/Ort Thema Veranstalter/Kontakt
Termine und Veranstaltungen
Journal für Kulturpflanzen 71. 2019
228
Term
ine und Veranstaltungen
27. – 28.01.2020
Braunschweig
33. Tagung 
des DPG-Arbeitskreises 
Krankheiten im Getreide und Mais
https://plant-protection.net/de/
arbeitskreise/getreidekrankheiten/ 
Veranstalter: Bayr. Landesanstalt für 
Landwirtschaft 
Kontakt: Helmut Tischner 
E-Mail: helmut.tischner@lfl.bayern.de
28. – 31.01.2020
Essen
IPM Essen https://www.ipm-essen.de
11. – 12.02.2020
Braunschweig
32. Jahrestagung 
des Arbeitskreises Raps
https://plant-protection.net/de/
arbeitskreise/raps/ 
Veranstalter: LWKN, JKI 
Kontakt: Holger Kreye, Meike Brandes 
E-Mail: 
holger.kreye@lwk-niedersachsen.de; 
meike.brandes@julius-kuehn.de
11. – 13.02.2020
Tulln, Österreich
Plant Breeding Symposium (GPBC):
„Digital Breeding“
https://gpz2020.boku.ac.at/ 
Veranstalter: GPZ 
Kontakt: Susanne Weber 
E-Mail: gpz2020@boku.ac.at
12. – 13.02.2020
Braunschweig
30. Tagung 
des DPG-Arbeitskreises Schädlinge 
in Getreide, Mais und Leguminosen
https://plant-protection.net/de/
arbeitskreise/getreideschaedlinge/ 
Veranstalter: DPG 
Kontakt: Stefan Krüssel
18. – 19.02.2020
Bernburg
30. Bernburger Winterseminar 
für Arznei- und Gewürzpflanzen
http://dpg.phytomedizin.org/de/termine
03. – 05.03.2020
Braunschweig
29. Deutsche Arbeitsbesprechung 
über Fragen der Unkrautbiologie und 
-bekämpfung (Unkrauttagung)
https://www.unkrauttagung.de/ 
Veranstalter: DPG, JKI, TU Braunschweig
E-Mail: unkrauttagung@julius-kuehn.de
03. – 05.03.2020
Braunschweig
32. Jahrestagung 
des Arbeitskreises Herbologie
https://plant-protection.net/de/
arbeitskreise/herbologie/ 
Veranstalter: 
Kontakt: Jan Petersen, Jörg Becker 
E-Mail: petersen@fh-bingen.de, 
Becker1@dow.com
Datum/Ort Thema Veranstalter/Kontakt


German

Termine und Veranstaltungen

Jahresinhaltsübersicht 2018

Schulz, Hartwig

Blank, Catharina

Böttcher, Christoph

Fürstenau, Benjamin

Krähmer, Andrea

Meiners, Torsten

Riewe, David

English

2nd International Plant Spectroscopy Conference (IPSC - 2019)

Devaux, Marie-Françoise

Corcel, Mathias

Guillon, Fabienne

Saulnier, Luc

Barron, Cécile

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
33 
02-03: Autofluorescence multispectral image analysis at the macroscopic scale for 
tracking wheat grain tissues: a novel approach for a more specific identification of 
wheat grain dietary fibre 
Marie-Françoise Devaux1, Mathias Corcel1, Fabienne Guillon1, Luc Saulnier1, Cécile Barron2 
1UR 1268 BIA, INRA, Rue de la Géraudière, 44 316 Nantes, France Country 
2UMR IATE, INRA, Montpellier Supagro, Université Montpellier, CIRAD, Montpellier, France 
E-mail: fabienne.guillon@inra.fr 
Wheat grain contains about 12-14% of fibres mainly located in the outer layers. The 
composition and the structure of wheat dietary fibres, as well as the nature and amount of 
co-passengers, vary according to the tissue where they are originated from. The aleurone 
layer is rich in low substituted arabinoxylans esterified to ferulic acid whereas outer pericarp 
contains highly substituted arabinoxylans but also cellulose and lignin. Consequently wheat 
dietary fibres properties showed a high variability according to their tissue of origin within 
the grain, which deeply impact their nutritional effects. If the identification of tissues in 
wheat grain is commonly performed, it remains challenging for food ingredient such as mill 
streams (flour, bran etc).  
Equipements are now available to acquire multispectral fluorescence images at the 
macroscopic scale using filters with specific excitation/emission wavelengths. These 
fluorescence macroscopes allow obtaining images of a representative number of particles 
together with a spatial resolution of less than 3 µm. In such images, the intensities measured 
for each pixel, though they are not spectra, can be assembled to form spectral profiles. To 
identify the tissular origin from this information, we propose to develop a prediction model 
on particles using calibration data coming from the observation of tissue sections. This 
approach is based on several assumptions. The first one is that the multispectral 
autofluorescence of plant tissues is specific and the second is that it is possible to measure 
fluorescence intensities in a reproducible way. The objective of the present work was to 
check the fluorescence macroscope as an efficient device for measuring and comparing 
fluorescence intensities. 
The variability of fluorescence profiles was studied by selecting pixels in cross-sections or in 
particles mounted in air or in water. The statistical variations were studied by principal 
component analysis and variance analysis. The first effect, mainly described by principal 
component 1, was to differentiate aleurone layer from pericarp tissue. The second effect, 
mainly described by component 2, was a difference between the two mounting media. The 
differences between sections or powders were not correlated to the other factors and were 
considered as not significant. Our results show that profiles extracted from multispectral 
images of cross-sections or particles are similar and allow the identification of wheat grain 
tissues. If implemented, the prediction from cross-section could be less tedious than other 
methods requiring dissection and lead to the identification of more tissues. We have 
demonstrated the proof of concept of tracking wheat dietary fibre origin by predicting 
tissues on images of particles. This method could help to better qualify flours and various 
milling fractions as well as to control whole grain products.  


Autofluorescence multispectral image analysis at the macroscopic scale for tracking wheat grain tissues: a novel approach for a more specific identification of wheat grain dietary fibre

Zude-Sasse, Manuela

Regen, Christian

East, Andrew

Mo, Li

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
85    
P-007: Measuring absorption and reduced scattering coefficients of fresh fruit by 
means of laser-induced backscattering imaging 
Manuela Zude-Sasse1, Christian Regen1, Andrew East2, Li Mo2 
1Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB) 14469 Potsdam, Germany 
2Massey AgriTech Partnership, Massey University, Palmerston North, New Zealand 
E-mail: mzude@atb-potsdam.de 
 
Decoupling optical properties of fruit and vegetables appears challenging, but necessary to 
get better insight of the relationship between light and produce attributes. In this study, nine 
solid phantoms were applied capturing the ranges of absorption (µa) from 0.1 to 1.5 cm-1 and 
reduced scattering (µs’) from 2.1 to 22.1 cm-1 found earlier in fresh fruit. Phantoms were 
analysed non-destructively using laser-induced backscattering imaging (LLBI) at 660 nm. 
Data analysis of LLBI was carried out on the diffuse reflectance, attenuation profiles obtained 
by means of Farrell’s diffusion theory either calculating µa [cm-1] and µs’ [cm-1] in one fitting 
step or fitting only one optical variable and providing the other one from a destructive 
analysis. The nondestructive approach was approved when calculating one unknown 
coefficient non-destructively, while no ability of the method was found to analysis both, µa 
and µs’, non-destructively. Measuring uncertainty decreased when µs’ was fixed, while µa was 
non-destructively calculated. The approach was tested on fresh fruit. Results indicated that 
the optical properties of fruit changed in correspondence to chlorophyll and water contents. 
A batch-wise calibration step of µs’ and online analysis of µa may be considered in future 
developments for more robust fruit sorting results, when considering real fruit developed 
from carpels showing high variability of µs’ such as stone fruit and berries. 
 
  


Measuring absorption and reduced scattering coefficients of fresh fruit by means of laser-induced backscattering imaging

Kicherer, Anna

Herzog, Katja

Töpfer, Reinhard

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
86    
P-008: Sensor application in grapevine breeding 
Anna Kicherer, Katja Herzog, Reinhard Töpfer 
Institute for Grapevine Breeding Geilweilerhof, Julius Kühn-Institut, Siebeldingen, Germany 
 
Due to its perennial nature and size, the acquisition of phenotypic data in grapevine research 
is for the most part restricted to the field. Techniques to assess phenology and morphology 
traits are mostly based on visual scoring. Some traits like biotic and abiotic stress and 
especially quality traits are evaluated by invasive measurements. The new arising sensor 
technologies make non-destructive evaluations of phenotypic traits available for grapevine 
research by using different sensors and sensor platforms, from greenhouse and lab 
application to the field application. Varying outdoor light conditions and background used 
to be the biggest environmental impact and challenge for field phenotyping of grapevines. 
Facing these problems the presented Phenoliner is a new type of ground based, robust field 
phenotyping platform. Following the concept of a movable tunnel, the vehicle is based on a 
grape harvester. It is equipped with different sensor systems within the tunnel (multi-camera 
system, hyperspectral cameras) and above (RTK-GPS, orientation and speed sensors). 
Through an artificial light source in the tunnel it is independent from external light 
conditions. In combination with the artificial background the Phenoliner allows standardised 
acquisition of high-quality, geo-referenced sensor data. The multi-camera system is used for 
the automated acquisition of colored 3D data of multiple vine rows for the automated 
calculation of yield parameters (number of grape bunches and berries, berry size) to be used 
for yield prediction. The hyperspectral cameras are used to detect spectral data in a broad 
range of spectral bands covering a spectrum from 400 nm to 2,500 nm to evaluate e.g the 
health status. 
The Phenoliner can be used for a high-throughput, automatic and non-invasive acquisition 
of phenotypic data directly in the field. It allows a fast, robust and precise screening of 
grapevines for several traits. The given platform can be extended through further sensors at 
any given time. The hyperspectral system is also used for lab screening applications. Further 
phenotyping tools, e.g. based on 3D point clouds, can be used to describe cluster 
architecture or other traits thus improving breeding efficiency. 
 
  


Sensor application in grapevine breeding

Krieger, Jorita

Gerighausen, Heike

Lilienthal, Holger

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
88    
P-010: Penotyping of winter oilseed rape with sensor systems under field conditions 
Jorita Krieger, Heike Gerighausen, Holger Lilienthal 
Julius Kühn-Institut (JKI), Institute for crop and soil science, Brunswick, Germany 
E-mail: jorita.krieger@julius-kuehn.de 
 
Plant breeding, especially conventional as well as marker assisted selection of suitable 
genotypes requires labor and time consuming screening of large amounts of plant material 
in field trials. In order to speed up the phenotyping process and to increase the number of 
genotypes being tested, new sensor systems have to be introduced. 
Innovative non-destructive phenotyping methods to capture factors of yield formation in 
oilseed rape are used in the interdisciplinary project RapiD. 
Within the scope of this project, field trials with winter oilseed rape were conducted at the 
research station in Brunswick. High phenotypic variability was achieved by plant material 
that differed in architecture of the crop stand. Therefore nine (season 2017/18) and 
16 (season 2018/19) different genotypes, respectively, were tested under 120 and 170 kg ha-1 
of mineral nitrogen. During season, plant traits, like field emergence, plant development, 
beginning of flowering, plant high, amount of flowers and pods, leaf area index and biomass 
were measured regularly the conventional way. In parallel different spectral sensors (RGB, 
multispectral, hyperspectral and laserscanning) have been tested and calibrated to 
destructive field sampling. In a next step sensors will be used on unmanned aerial vehicles 
(UAV) in order to speed up the phenotyping process. 
The authors like to acknowledge the funding by the Federal Ministry of Food and Agriculture 
(FKZ: 22021615). 
 
  


Penotyping of winter oilseed rape with sensor systems under field conditions

Brangule, Agnese

Tretjakovs, Pẽteris

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
104    
P-019: Herbal characterization and discrimination perspectives using Fourier 
transform infrared photoacoustic spectroscopy (FTIR PAS) and diffuse reflectance 
infrared spectroscopy (DRIFT) 
Agnese Brangule, Pẽteris Tretjakovs 
Riga Stradiņš University, Department of Human Physiology and Biochemistry, Riga, Latvia 
E-mail: agnese.brangule@rsu.lv 
 
This study demonstrates the significant potential of cantilever-enhanced Fourier transform 
infrared photoacoustic spectroscopy (FTIR PAS) principles and diffuse reflective infrared 
spectroscopy (DRIFT) with a diamond stick. The improved sensitivity and reproducibility of 
both methods present an absolute tool in the study of herbals and plants.  
 
For many centuries, herbal medicine (HMs) has been used worldwide in traditional health 
care [1]. FTIR methods have been widely used since the 1960s [2] and can be used for both 
qualitative and quantitative analysis [3]. In the field of HMs, the FTIR fingerprint spectra have 
been used since early 1987 and are used less frequently than chromatography methods (CM) 
[4,5,6]. Until now, the introduction of FTIR methods was limited by the complexity of spectra 
and its interpretation. This problem can be resolved by using chemometrics and machine 
learning. The major advantages of FTIR methods are the following: it is sensitive and non-
destructive or only slightly damages the sample; it requires minimal sample preparation at 
most; small sample quantities are necessary for measuring [2,3]. One of the important 
features of FTIR is the possibility to simultaneously determine different components in the 
same sample from a single instrumental measurement [7]. In previous studies, the FTIR 
spectroscopy’s sampling methods transmission and ATR was most widely used as DRIFT and 
cantilever-enhanced photoacoustic spectroscopy (FTIR PAS). FTIR PAS is based on the 
photoacoustic effect. If the substance is irradiated with a pulsating light, the substance emits 
acoustic waves that have the same frequency as the pulsating light. 
In this study, we evaluated dried herbals and green, and black tee (Figure 1). PAS and DRIFT 
spectra were taken at 450–4000 cm-1, at a resolution of 4 cm-1, and an average made from 
10 scans. For PAS, the homogenized samples were placed in the PAS cell filled with helium 
gas (flow 0.5 l/min), but for DRIFT homogenized samples were placed on the diamond stick. 
 
Figure 1. FTIR PAS and FTIR DRIFT spectra of green tea and chamomile 
2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
105    
Comparison between spectra recorded by PAS and DRIFT showed high sensitivity and good 
resolution. The results obtained provide information about the spectral behavior of 
homogenized herbal and tee powder can be a useful for establishing identification and 
discrimination criteria. It has been demonstrated that PAS and DRIFT can be useful 
experimental tool for the characterization and discrimination of herbals. 
References 
[1] WANG, P., et al., 2015: J. Pharm. Anal., 5, 277–284. 
[2] SMITH, B.C., 2011: CRC Press, 20‒65. 
[3] STUART, B.H., 2005: Infrared spectroscopy: Fundamentals and Applications. In: Analytical Techniques 
in the Sciences. 
[4] RIOLO, D., et al., 2018: J. Pharm. Biomed. Anal., 5, 329-334. 
[5] YUAN, J.R., et al., 1987: J. of Shandong University of Traditional Chinese Medicine, 11, 58–63. 
[6] SCOPUS keywords: Herbal and Medicine and Analysis; No. of papers: 13 511; Refinement: FTIR (126 
papers; only in last 10 years 12-18 papers per year) – [retrieved 16/09/2018] 
[7] BANSAL, A., et al., 2014: J. Pharm. Anal., 4, 223–233. 
 
  


Herbal characterization and discrimination perspectives using Fourier transform infrared photoacoustic spectroscopy (FTIR PAS) and diffuse reflectance infrared spectroscopy (DRIFT)

Kowalczyk, Mariusz

Krzyżanowska-Kowalczyk, Justyna

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
111    
P-024: Untargeted multiplatform metabolomics assay for the analysis of plant-
herbivore interactions in broad bean 
Mariusz Kowalczyk, Justyna Krzyżanowska-Kowalczyk 
Institute of Soil Science and Plant Cultivation, State Research Institute, Puławy, Poland 
E-mail: mkowalczyk@iung.pulawy.pl 
 
Orthogonal separation techniques are frequently employed in metabolomics to extend the 
range of analyzed metabolites. Such approaches are often multi-instrumental because 
different separation techniques sometimes require different interfaces with the mass 
spectrometer and not all of them are universally available. In untargeted metabolomics, the 
use of various instruments and different acquisition modes can introduce undesired 
variability in the obtained datasets. Also, some of the published strategies for multiplatform 
metabolomics are based on individual extractions of metabolites for each separation 
technique, frequently with different sets of internal standards and quality control samples. As 
a result, multiplatform methods are perceived as difficult and costly, both regarding the 
financial expenses and labor. However, for applications of untargeted metabolomics in plant 
sciences, they are usually necessary because of the vast numbers and structural 
heterogeneity of investigated compounds. Another problem often encountered in plant 
metabolomics is a broad diversity of observed metabolite concentrations, sometimes 
spanning a few orders of magnitude, which can generate non-quantitative results due to 
either saturated or below the detection limit signals. 
To address some of these concerns, we developed a method of metabolome analysis based 
on a single extraction and, at the same time, initial fractionation of metabolites from the 
30 mg of powdered plant material. Obtained fractions were then analyzed using a 
combination of separation techniques: RP-UHPLC, HILIC-UHPLC, GC, and CE. The same 
detector, high-resolution QTOF mass spectrometer, was used in each case, although with 
two different ionization modes. LC of polar and semi-polar compounds, as well as CE 
separations, were interfaced using ESI while APCI was used for GC analyses. Additionally, CE 
separations were carried out using three different capillaries and buffer systems. Overall, 
nearly 600 metabolites were annotated and subsequently used as a dataset to investigate 
the plant-herbivore interactions in broad bean seeds. 
 
This research is supported by the NCN grant 2014/15/B/NZ9/04302. 
 
  


Untargeted multiplatform metabolomics assay for the analysis of plant-herbivore interactions in broad bean

Lyu, Hui

Xu, Shu

Chen, Yu

Paetz, Christian

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 
121 
P-033: Secondary metabolites in seed development of Musella lasiocarpa 
Hui Lyu1, Shu Xu2, Yu Chen2, Christian Paetz1 
1Max-Planck-Institute for Chemical Ecology, Jena, Germany 
2 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China  
E-mail: hlyu@ice.mpg.de, chenyu@cnbg.net, shuxu@cnbg.net, cpaetz@ice.mpg.de 
Musella lasiocarpa, a member of the Musaceae (Fig.1), is an endangered endemic banana 
species in Southwestern China [1]. The plant has no importance as food source but it is well-
known as an ornamental plant. We were interested in the built-up of secondary metabolites 
in the seeds during their development to complement the knowledge on developmental 
biology of Musa species [2]. Seeds of different developmental stages were sampled and 
analyzed for their metabolic profiles by high performance liquid chromatography coupled 
with high-resolution electrospray ionization mass spectrometry (HPLC-HRESIMS) and 
fluorescence detection (FLD). The identity of metabolites was elucidated by means of nuclear 
magnetic resonance spectroscopy (NMR) which eventually enabled us to construct a 
timetable of emerging metabolites formed during seed development.  
Figure 1: Musella lasiocarpa (Musaceae) 
References 
[1] JIE, T., 2008: Studies on the Reproductive Biology of Musella lasiocarpa. 
[2] GRAVEN, P., et al., 1996: Structure and Macromolecular Composition of the Seed Coat of the 
Musaceae. Ann. Bot-London, 77, 105-122. 


Secondary metabolites in seed development of Musella lasiocarpa

Julius Kühn-Institut

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