E-Journal Faculty of Medicine Universitas Padjadjaran / E-Jurnal Fakultas Kedokteran Universitas Padjadjaran
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PERBANDINGAN EFEK FENTANYL DENGAN KETAMIN TERHADAP SKOR PEMULIHAN PASCAANESTESI UMUM PADA OPERASI ODONTEKTOMI DIUKUR DENGAN QoR-40 DI RUMAH SAKIT UMUM PUSAT Dr HASAN SADIKIN BANDUNG
No full textPenggunaan fentanil pada anestesi umum memiliki pengaruh terhadap pemulihan pascaoperasi danpenurunan hemodinamik saat induksi. Metode anestesi umum menggunakan analgetik nonopioiddiharapkan meningkatkan kualitas pemulihan pascaoperasi. Pemberian ketamin dosis subanestesi memberi efek analgetik dengan efek samping minimal serta perubahan tekanan darah dan nadi lebih stabil. Tujuan penelitian ini membandingkan efek fentanil dengan ketamin terhadap kualitas pemulihan serta perubahan tekanan darah dan nadi saat induksi. Penelitian ini merupakan uji klinis tersamar ganda pada 30 pasien yang menjalani operasi odontektomi dengan anestesi umum di Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung dari Januari–Maret 2020. Pasien dibagi menjadi 2 kelompok, yaitu kelompok ketamin, diinduksi menggunakan ketamin 0,5 mg/kgBB dan kelompok fentanil, diinduksi menggunakan fentanil 1,5 mcg/kgBB. Data dianalisis dengan uji-t tidak berpasangan, Uji Mann Whitney, dan Uji Kolmogorov-smirnov dengan nilai p<0,05 dianggap bermakna. Terdapat penurunan tekanan darah dan nadi yang signifikan (p<0,05) di menit ke-1, 3, dan 5 pada grup fentanil. Penilaian QoR-40 pada kelompok ketamin memiliki angka lebih tinggi (181,07±5,32) dibanding dengan kelompok fentanil (176,60±2,59) secara bermakna (p<0,05). Simpulan, skor pemulihan pascaanestesi umum dengan ketamin lebih tinggi dibanding dengan fentanil pada operasi odontektomi yang dinilai dengan QoR-40 dan ketamin dengan dosis subanestesi saat induksi menunjukkan hemodinamik yang lebih stabil dibanding dengan induksi menggunakan fentanil. Comparison of Fentanyl and Ketamine’s Effects on Post-General Anesthesia Recovery Scores Measured by QoR-40 and Changes in Blood Pressure and Pulse in Odontectomy SurgeryThe use of fentanyl in general anesthesia has side effects that may prolong postoperative recovery and hemodynamic decline when induction. Methods of general anesthesia without fentanyl may improve the quality of postoperative recovery. Ketamine in subanesthetic doses has analgesic effects with minimal side effects and more stable blood pressure and pulse changes. This study aimed to compare fentanyl and ketamine’s effects on quality of recovery and changes in blood pressure and pulse. This was a double-blinded clinical study in 30 patients with odontectomy under general anesthesia in Dr. Hasan Sadikin General Hospital from January–March 2020. Patients were divided into two groups, a ketamine group, induced using 0.5 mg/kgBW ketamine, and a fentanyl group, induced using 1,5 mcg/kgBW fentanyl. Data were analyzed using the unpaired t-test, Mann Whitney, and Kolmogorov-Smirnov test, a p-value of <0.05 was considered significant. Values of QoR-40 in the ketamine group had a significantly (p<0.05) higher value (181.07±5.32) compared to the fentanyl group (176,60±2,59). In conclusion, the quality of post-general anesthesia recovery using ketamine is higher than fentanyl in odontectomy evaluated using QoR-40. Induction using subanesthetic doses of ketamine shows more stable hemodynamic than fentanyl.ABSTRAK Penggunaan fentanyl pada anestesi umum memiliki efek samping yang dapat memperpanjang pemulihan pascaoperasi. Metode anestesi umum tanpa menggunakan fentanyl dapat meningkatkan kualitas pemulihan pascaoperasi. Ketamin dengan dosis subanestesi memiliki efek analgetik dan efek samping minimal. Tujuan penelitian ini membandingkan efek fentanyl dan ketamin terhadap kualitas pemulihan. Metode penelitian dengan uji klinis tersamar ganda pada 30 pasien yang menjalani operasi odontektomi dengan anestesi umum di rumah sakit umum pusat Hasan Sadikin dari Januari 2020 – Maret 2020. Pasien dibagi menjadi 2 kelompok yaitu kelompok ketamin, diinduksi dengan menggunakan ketamin 0.5 mg/kg dan kelompok fentanyl, diinduksi dengan menggunakan fentanyl 1,5 mcg/kg. Pada 24 jam pascaoperasi, dilakukan wawancara terhadap sampel untuk mengisi kuesioner QoR-40 untuk mengevaluasi kualitas pemulihan pascaoperasi. Data dianalisis dengan uji t tidak berpasangan, uji mann whitney dan uji kolmogorov-smirnov, nilai p<0.05 dianggap bermakna. Angka penilaian QoR-40 pada kelompok ketamin memiliki angka lebih tinggi (181.07±5.32) dibandingkan dengan kelompok fentanyl (176,60±2,59) secara bermakna (p<0.05). Simpulan penelitian ini kualitas pemulihan pascaanestesi umum dengan ketamin lebih tinggi dibandingkan fentanyl pada operasi odontektomi yang dinilai dengan QoR-40
Factors Affecting Nutritional Status among Children Aged 12–23 Months
Get PDFBackground: In Indonesia about 18% of children, mostly in the first two years of life, are malnourished, causing a serious impact. Many factors are thought to affect nutritional status among young children. This study was conducted to determine factors affecting the nutritional status of children aged 12–23 months in West Java, Indonesia.Methods: This cross-sectional study used secondary data obtained from a Basic Health Research (Riset Kesehatan Dasar, RISKESDAS) 2010, conducted by Badan Penelitian dan Pengembangan Kesehatan (BALITBANGKES) Ministry of Health Republic of Indonesia. All of the data among children aged 12–23 months in West Java province was obtained. Statistical relationships between predisposing factors and nutritional status i.e. weight for height were analyzed using the chi-square test.Results: In total, 730 data were obtained, of which 567 data met the inclusion criteria. Malnutrition was detected in 101 (17.8%) children. There was a significant relationship (p=0.017, OR=2.6, 95% CI 1.1–5.8) between low birth weight and nutritional status. Nevertheless, no significant relationship was found between nutritional status and history of exclusive breastfeeding (p=0.629), complimentary feeding (p=0.949), vitamin A (p=0.209), infectious diseases (p=0.266), complete immunization status (p=0.420), and mother education level (p=0.251).Conclusions: The low birth weight is the only significant factor associated with the nutritional status among children less than 2 years old; resulting in that low birth weight had a 2.6 higher chance of malnutrition in the early years. Other factors are unexpectedly not significant in this study. There is thus a need to improve the quality of programs, focusing on childbearing mothers, during antenatal control
Histopathological Effects of Omega-3 in Reducing Cartilage Destruction Progression in Mice with Knee Joint Osteoarthritis
Get PDFrimary and secondary osteoarthritis relates to inflammatory processes and inflammatory mediators and is destructive to the articular cartilage. Omega-3 is known to be an alternative treatment for rheumatoid arthritis due to the anti-inflammatory effect. This study is an experimental study with simple random sampling using 36 Wistar mice, which were divided into an intervention group and a control group, to understand the effect of omega-3 in slowing progress cartilage destruction in knee joint with osteoarthritis. This study is performed at the Clinical Pharmacology Laboratory, Faculty of Medicine, Universitas Padjadjaran, and the Anatomical Pathology Laboratory, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, in November 2018. Osteoarthritis was induced using intraarticular 1 mg monosodium iodoacetate injection to the mouse knee joint. The intervention group received oral omega-3 every day while the control group did not. Samples from the knee joint were extracted to evaluate the cartilage destruction histopathologically. Results were then analyzed using the Mann-Whitney test and a significant difference of the osteoarthritis grades was identified between the intervention group and the control group on day 7 (p=0.003), day 14 (p=0.003), and day 21 (p=0.003). In addition, a significant difference in the osteoarthritis grading changes was also found between the study group and the control group on day 7 and day 21 (p=0.004). Hence, omega-3 has the ability to slow down the histopathological cartilage destruction progress in mice with knee joint osteoarthritis.Efek Pemberian Omega-3 terhadap Perlambatan Progresivitas Destruksi Kartilago Sendi Lutut Tikus yang Mengalami Osteoartritis Secara HistopatologisOsteoartritis primer dan sekunder berhubungan dengan proses inflamasi dan mediator inflamasi dan merusak tulang rawan artikular. Omega-3 dikenal sebagai pengobatan alternatif untuk rheumatoid arthritis karena efek anti-inflamasi. Penelitian ini adalah penelitian eksperimental dengan pengambilan sampel acak sederhana menggunakan 36 tikus Wistar, yang dibagi menjadi kelompok intervensi dan kelompok kontrol, untuk memahami efek omega-3 dalam memperlambat perkembangan kerusakan tulang rawan pada sendi lutut dengan osteoarthritis. Penelitian ini dilakukan di Laboratorium Farmakologi Klinik, Fakultas Kedokteran, Universitas Padjadjaran, dan Laboratorium Patologi Anatomi, Fakultas Kedokteran Universitas Padjadjaran/Dr. Rumah Sakit Umum Hasan Sadikin Bandung, pada November 2018. Seluruh hewan uji dilakukan induksi osteoartritis dengan monosodium iodoasetat sebanyak 1 mg yang disuntikkan ke dalam sendi lutut. Kelompok perlakuan diberikan omega-3 1 kali per hari per oral, sedangkan kelompok kontrol tidak diberikan omega-3. Sampel jaringan sendi lutut diambil dan dilakukan penilaian destruksi kartilago secara histopatologis. Hasil kemudian dianalisis dengan menggunakan uji Mann-Whitney dan perbedaan yang signifikan dari nilai osteoartritis diidentifikasi antara kelompok intervensi dan kelompok kontrol pada hari 7 (p=0,003), hari 14 (p=0,003), dan hari 21 (p=0,003). Selain itu, perbedaan yang signifikan dalam perubahan penilaian osteoarthritis juga ditemukan antara kelompok studi dan kelompok kontrol pada hari ke 7 dan hari ke 21 (p=0,004). Oleh karena itu, omega-3 memiliki kemampuan untuk memperlambat progres destruksi tulang rawan histopatologis pada tikus dengan osteoartritis sendi lutut.
Pattern of Indirect Immunofluorescence Assay Antinuclear Antibody in Pediatric Lupus Nephritis
Get PDFObjective: To determine the association between Anti-Nuclear Antibody (ANA) pattern in pediatric Systemic Lupus Erythematosus (SLE) patients and proteinuria as a sign of renal involvement in SLE.Methods: This was a cross-sectional study, using data from medical records involving 89 newly diagnosed with SLE (aged ≤ 18 years) in Department of Child Health Dr. Hasan Sadikin General Hospital, Bandung, from January 1st 2018 to June 30th 2019. Data of ANA pattern and proteinuria were collected from medical record. ANA pattern was examined by Immunofluorescence Assay (IFA) method. Chi-square was used to analyze the association between ANA pattern and proteinuria as a sign of renal involvement in pediatric SLE patients.Results: There were 89 patients, consisting of 7 male (7.9%) and 82 female (92.%) with median age of 14 (IQR=12-16). There were only 44 pattern of ANA as follow: homogenous 56.8%, speckled 36.4%, and nucleolar 6.8%. However, there is no significant association between homogenous pattern with proteinuria events (p=0.831).Conclusions: Homogenous pattern was the most frequent ANA pattern in children with SLE, and the pattern has no association with proteinuria events
Correlation Between HbA1c and Albuminuria in Type 2 Diabetes Mellitus
Get PDFIn patients with type 2 diabetes mellitus (Type 2 DM), glycemic control plays an essential role in reducing macro- and microvascular complications. The earliest marker for diabetic nephropathy is the presence of albuminuria. Meanwhile, HbA1c has been recognized as a marker of glycemic control in the blood. This study aimed to identify the correlation between HbA1c and albuminuria in type 2 DM. This was a cross-sectional study involving 100 patients with type 2 DM aged 40–70 years visiting Dr. Soetomo Hospital, Surabaya, Indonesia. Secondary data from medical records of type 2 DM patients undergoing HbA1c and albuminuria examinations from January to December 2015 were used. The mean age of subjects was 55.4 ±8.7 years, with the majority (55%) being of female gender. The mean HbA1c concentration was 9.8±2.4%. Most subjects (83%) had an HbA1c level of ≥7 %. Albuminuria was found in the majority of the subjects (78%), where 33% of them had positive 1 albuminuria. The Spearman correlation test showed a positive strong significant correlation between HbA1c and albuminuria, with r=0.865 and p<0.001. Thus, it can be concluded that there is a significant correlation between HbA1c and albuminuria in type 2 DM.Korelasi HbA1c dan Albuminuria pada Diabetes Melitus Tipe 2 Pada penderita diabetes melitus tipe 2 (DM tipe 2), kontrol glikemik memegang peranan penting untuk menurunkan komplikasi makrovaskular dan mikrovaskular. Petanda awal terjadinya nefropati diabetik adalah adanya albuminuria. HBA1c merupakan petanda untuk mengetahui kontrol glikemik didalam darah. Tujuan penelitian ini untuk mengetahui korelasi antara HbA1c dengan albuminuria pada DM tipe 2. Penelitian ini menggunakan desain potong lintang, mengikutsertakan 100 penderita DM tipe 2 berusia 40–70 tahun di Rumah Sakit Dr. Soetomo Surabaya. Data yang digunakan adalah data sekunder dari rekam medis penderita DM tipe 2 yang melakukan pemeriksaan HbA1c dan albuminuria pada bulan Januari 2015 sampai dengan Desember 2015. Rerata usia subjek adalah 55,47±8,7 tahun dengan subjek terbanyak berjenis kelamin perempuan (55%). Rerata kadar HbA1c berkisar 9,8±2,4 %. Sebagian besar subjek mempunyai kadar HbA1c ≥7 % yaitu 83%. Albuminuria dijumpai pada sebagian besar subjek yaitu 78% dan sebagian besar subjek mempunyai albuminuria positif 1, yaitu 33 %. Hasil uji korelasi Spearman menunjukkan terdapat korelasi positif kuat yang bermakna antara HbA1c dengan albuminuria dengan nilai r=0,865 dan p<0,001. Dengan demikian, dapat disimpulkan terdapat korelasi yang bermakna antara HbA1c dan albuminuria pada DM tipe 2
Hubungan Profil Lipid (Kadar Trigliserida, HDL, LDL, Total Kolesterol) dengan Jenis Infeksi Bakteri pada Pasien Sepsis
Get PDFGangguan profil lipid berhubungan dengan peningkatan kejadian gagal organ dan kematian pada pasiensepsis. Gangguan profil lipid mungkin berbeda antarjenis infeksi bakteri (gram positif vs gram negatif),perbedaan ini mungkin jadi disebabkan oleh perbedaan klirens endotoksin yang berbeda dan mekanisme imunologis host yang berkaitan dengan metabolism lipid. Tujuan, menganalisis perbedaan profil kadar lipid (HDL, LDL, Trigliserida, total kolesterol) dengan jenis infeksi bakteri pada pasien sepsis di ruang perawatan intensif RSUD Dr. Soetomo yang dilaksanakan pada bulan April–Juli 2020. Penelitian ini merupakan penelitian analitik observasional pada pasien dewasa yang memenuhi kritera sepsis-3. Jenis infeksi bakteri diklasifikasikan menjadi infeksi bakteri gram negatif, infeksi bakteri gram positif, infeksi campuran, dan tidak tumbuh kuman. Penelitian ini menganalisis perbedaan profil kadar lipid antarjenis infeksi bakteri menggunakan analisis normalitas data dan analisis varian data. Hasil dari 38 pasien sepsis dewasa pada penelitian ini didapatkan 13 pasien infeksi bakteri gram negatif, 13 pasien infeksi gram positif, 5 pasien infeksi campuran gram positif dan negatif, dan tidak didapatkan pertumbuhan kuman pada 7 pasien. Kadar HDL lebih rendah pada infeksi bakteri gram negatif dan infeksi bakteri campuran gram positif-gram negatif (p<0,05). Kadar trigliserida lebih tinggi pada infeksi bakteri gram negatif dan infeksi bakteri campuran gram positif - gram negatif (p<0,05). Tidak terdapat perbedaan bermakna kadar LDL dan total kolesterol antarjenis infeksi bakteri. Simpulan, kadar HDL plasma lebiH rendah dan kadar trigliserida lebih tinggi pada pasien sepsis infeksi bakteri gram negatif dan bakteri campuran gram positif–gram negatif disbanding dengan pasien sepsis infeksi bakteri gram positif dan tidak didapatkan pertumbuhan kuman. Relationship of Lipid Profile (Levels of Triglyceride, HDL, LDL, Total Cholesterol) with Types of Bacterial Infection in Sepsis PatientsImpaired lipid profiles are associated with an increased incidence of organ failure and mortality in septic patients. Disorders of the lipid profile may differ between bacterial infection types (gram-positive vs gram-negative). Differences could be due to dissimilarities in different endotoxin clearance and host immunological mechanisms related to lipid metabolism. The study analyzed differences in lipid profiles (HDL, LDL, triglycerides, total cholesterol) with the bacterial infection type in septic patients in the intensive care room of Dr. Soetomo Hospital in April–July 2020. This study was an observational analytic study of 38 adult patients who met the criteria for sepsis-3. Bacterial infection types were classified into gram-negative bacterial infections, gram-positive bacterial infections, mixed infections and culture negative. This study analyzed differences in lipid profiles between bacterial infection types, and used data normality analysis and data variant analysis. Results showed that 13 patients had gram-negative bacterial infection, 13 patients were with gram-positive infection, five patients were with mixed gram-positive gram-negative infections, and seven patients had no germ growth. Plasma HDL levels were lower in gram-negative bacterial infections and mixed gram-positive gram-negative bacterial infections (p<0.05). Plasma triglyceride levels were higher in gram-negative bacterial infections and mixed gram-positive gram-negative bacterial infections (p<0.05). There was no significant difference in LDL levels and total plasma cholesterol between bacterial infections types. In conclusion, plasma HDL levels are significantly lower, and triglyceride levels were significantly higher in septic patients with gram-negative and mixed gram-positive bacterial infections than in sepsis patients with gram-positive bacterial infections and culture negative
Management of Juvenile Nasopharyngeal Angiofibroma in a Referral Hospital in West Java, Indonesia
Get PDFBackground: Juvenile nasopharyngeal angiofibroma (JNA) is a fibrovascular tumor originating from the supero-posterior area of the sphenopalatine foramen. This tumor is histologically benign, but is clinically malignant because JNA has potentially destructive spread extending to the skull base and the cranium. The aim of the study was to evaluate the management of JNA in Dr. Hasan Sadikin General Hospital Bandung, a referral hospital in West Java.Methods: This was a descriptive retrospective study using medical record data of JNA patients during the period of 2011-2018. The stage of JNA was designated, based on the FISCH classification.Results: There were112 JNA cases, consisting of 99% male and 1% female. Most of them (97%) were aged 10-20 years old (median16 years old). The chief complaints were nasal obstruction (46%) and recurrent epistaxis (36%). Most of the JNA stage was stage II (43%). Major feeding vessel of JNA was from an internal maxillary artery (IMAX) and ascending pharyngeal artery (28%). Preoperative management was equal for ligation and embolization. The complication of embolization was local pain (75%). The most surgical technique performed was the transpalatal approach (41.36%). Blood loss in a different stage in JNA surgery with embolization was less than surgery with ligation. There were patients (10%)with residual disease after 6 months follow-up.Conclusions: JNA is essentially a disease of the adolescent males. The management of JNA patients is surgery, mostly using transpalatal technique. Preoperative embolization of JNA is a safe procedure, minimizes intraoperative blood loss, decrease the incidence of residual or recurrence, and shortening the duration of the surgery.
Bleeding Patterns among Severe Hemophilia A and B Patients in West Java
Get PDFBackground: The clinical manifestations of hemophilia A (HA) and hemophilia B (HB)are quite similar; however, the bleeding characteristics of these two hemophilia types have been reported to be different. This study aimed to explore the bleeding patterns among patients with severe HA and severe HB.Methods: A cross-sectional study was conducted among patients with severe HA and HB registered at the West Java Indonesian Hemophilia Society. The inclusion criteria were patients with severe hemophilia diagnosed for at least one year. The bleeding patterns included bleeding episodes and bleeding types. The Mann-Whitney test was used to compare bleeding episodes and a chi-square test for bleeding types.Results: In total, 158 severe HA patients and 21 severe HB patients were included with a median bleeding frequency per patient per year for HA and HB was 24 (range 0–48) and 24 (range 5–48), respectively. The bleeding types in HA and HB were ecchymosis (69% vs. 66.7%), hematoma (62.7% vs. 61.9%), hemarthrosis (99.4% vs. 100%), epistaxis (46.8% vs. 38.1%), gum bleeding (87.3% vs. 95.2%), intracranial hemorrhage (15.2% vs. 9.5%), multiple hematomas (36.7% vs. 47.6%), hemarthrosis-hematoma (61.4% vs.61.9%), and hemarthrosis-ecchymosis (69% vs. 61.9%). However, there was no significant difference in all types of bleeding between HA and HB.Conclusions: There is no difference in the pattern of hemorrhage between severe HA and severe HB in West Java. However, the bleeding phenotypes in hemophilia has considerable implications in the therapeutic process. Further research is needed to optimize the treatment regimens
Lipid Level of Street Food at Cibeusi Elementary School in Jatinangor Subdistrict, West Java, Indonesia
Get PDFBackground: School-aged children have quite high energy consumption demand because they are growing and physically active. In Indonesia, school-aged children frequently consume their additional foods/snacks from street food vendors around their schools. This study aimed to reveal the lipid level of street food in Elementary Schools, in Jatinangor Subdistrict, West Java, Indonesia.Methods: This study was an observational descriptive study. Elementary Schools in Jatinangor Subdistrict were selected using a random sampling method. Four frequent street foods were fried meatball (in Indonesian: bakso goreng (basreng)), batagor (bakso tahu goreng), crepes, and egg roll were then taken, and lipid level was measured twice using Soxhlet extraction method.Results: Fried meatball contained 22.10% (±0.23%) or 2.78 gram lipid, batagor 30.091% (±4.14%) or 12.49 gram lipid, crepes 7.14% (± 0.12%) or 0.66 gram lipid, and egg roll 13.66% (±1.63%) or 1.28 gram lipid.Conclusions: Only the batagor fulfilled the lipid demand in one snack time. It is recommended to minimize the intake of batagor as a between-meal snack for a balanced nutrient intake control
Comparison of Antibacterial Efficacy between 96% Ethanolic Extracts from Abrus precatorius L. and Piper betle L. Leaves against Escherichia coli
Get PDFEscherichia coli (E.coli) is a frequently found infectious pathogen commonly transmitted through water. In Indonesia, the level of this pathogen exceeds the accepted standard. Several studies have shown the presence of antibiotic-resistant E.coli, making studies on alternative treatments for E.coli necessary. Abrus precatorius L. and Piper betle L. leaves are among herbs that have herbal antibacterial properties. This study observed and compared the antibacterial effects of Abrus precatorius L. and Piper betle L. leaves against E.coli. This was an in vitro experimental study performed at the Laboratory of the Department of Microbiology, Faculty of Medicine and Health Science, Atma Jaya Catholic University, from August to November 2019. Abrus precatorius L. and Piper betle L. leaves were extracted by maceration in 96% Ethanol, and further processed into concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. Disc-diffusion on Mueller-Hinton Agar was used to identify the inhibition zones of the extracts against E.coli ATCC 25922. Ciprofloxacin disc and 96% ethanol impregnated-discs were used as positive and negative controls, respectively. Independent t-test results showed a significant difference between Abrus precatorius L. and Piper betle L. leaves effects against E.coli with p=0.044 and p=0.045 (p<0.05), respectively. In conclusion, Abrus precatorius L. and Piper betle L. leaves have antibacterial effects against E.coli ATCC 25922, albeit less sensitivity than Ciprofloxacin, with Piper betle L. presents a greater effect than Abrus precatorius L.Perbandingan Efektivitas Antibakteri Ekstrak Etanol 96% Daun Abrus precatorius L. dan Daun Piper betle L. Terhadap Escherichia coliEscherichia coli (E.coli) adalah patogen infeksius yang sering ditemukan dan ditularkan melalui air. Di Indonesia, tingkat patogen ini melebihi standar yang diterima. Beberapa penelitian telah menunjukkan ada E.coli yang kebal antibiotik, membuat penelitian tentang pengobatan alternatif untuk E.coli diperlukan. Daun Abrus precatorius L. dan Piper betle L. berpotensi sebagai pengobatan herbal antibakteri. Penelitian ini bertujuan melihat dan membandingkan efek antimikroba kedua daun tersebut terhadap E.coli. Penelitian eksperimental in vitro ini dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Ilmu Kesehatan Atma Jaya mulai dari Agustus sampai November 2019. Daun Abrus precatorius L. dan Piper betle L. dimaserasi menggunakan etanol 96%, lalu dibentuk konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, dan 100%. Difusi cakram pada agar Mueller-Hinton dilakukan untuk menguji zona hambat kedua ekstrak terhadap bakteri E.coli ATCC 25922. Cakram ciprofloksasin merupakan kontrol positif, dan kontrol negatif adalah cakram yang direndam etanol 96%. Hasil uji independent t-test didapatkan perbedaan bermakna antara efektivitas ekstrak kedua daun terhadap E.coli, yaitu daun Abrus precatorius L. p=0.044 dan daun Piper betle L. p=0.045 (p<0.05). Simpulan, daun Abrus precatorius L. dan Piper betle L. memiliki efek antibakteri terhadap E.coli ATCC 25922 walaupun tidak lebih sensitif dari Ciprofloksasin dengan Piper betle L. memiliki efek yang lebih besar dibanding dengan Abrus precatorius L