727 research outputs found
Sort by
14C-based ΔffCO2 estimates and NOx measurements for Heidelberg (2020-2021)
No full textThese data files show Δ14CO2 and CO2 measurements, as well as 14C-based ΔffCO2 estimates of flask samples, and hourly-averaged in situ NOx concentrations from Heidelberg (HEI) for 2020 and 2021. Additionally, hourly modelled local NOx and ffCO₂ background concentrations for HEI are provided, based on the Stochastic Time-Inverted Lagrangian Transport (STILT) model and TNO emissions. This data is used to construct continous hourly ΔNOx-based ΔffCO2 estimates (Juchem et al., 2025)
Air Quality Alerts and Don't Drive Appeals: Evidence on Voluntary Pollution Mitigation Dynamics from Germany [Data and Replication Package]
No full textThis replication package provides data and Stata code to reproduce all results in "Air Quality Alerts and Don't Drive Appeals: Evidence on Voluntary Pollution Mitigation Dynamics from Germany" by Alexander Dangel and Timo Goeschl
Evaluation of Bioprinted Autologous Cartilage Grafts in an Immunocompetent Rabbit Model [data]
No full textThe current gold standard of auricular reconstruction involves a highly time-consuming manual graft assembly from autologous costal cartilage. The intervention may require multiple surgical procedures and lead to donor-site morbidity, while the outcome is highly dependent on individual surgical skills. A tissue engineering approach provides the means to produce cartilage grafts of a defined shape from autologous chondrocytes. The use of autologous cells minimizes the risk of host immune response; however, factors such as biomaterial compatibility and in vitro maturation of the tissue-engineered (TE) cartilage may influence the engraftment and shape-stability of TE implants. Here we tested the biocompatibility of bioprinted cartilage constructs containing autologous auricular chondrocytes in a rabbit model. The TE cartilage was produced by embedding autologous auricular chondrocytes into hyaluronan transglutaminase (HATG)-based bioink, previously shown to support chondrogenesis in human auricular chondrocytes in vitro and in immunocompromised xenotransplantation models in vivo. While the shape of the transplants was overall retained, drastic softening and loss of cartilage markers, such as sulfated glycosaminoglycans and collagen type II were observed. Furthermore, fibrous encapsulation and partial degradation of the transplanted constructs were indicative of a strong host immune response to the autologous TE cartilage. The current study thus illustrates the crucial importance of immunocompetent autologous animal models for the evaluation of TE cartilage function and compatibility
Insights for the Hydrogen-Mediated Deoxydehydration (DODH) with Cp*ReO3 and Alkene Extrusion of Related Re(V)-Diolates [data]
No full textExperimental Data to the manuscript Insights for the Hydrogen-Mediated Deoxydehydration (DODH) with Cp*ReO3 and Alkene Extrusion of Related Re(V)-Diolates:
To transform highly oxygenated molecules such as polyols from biomass into value-added chemicals, the deoxygenation via the deoxydehydration (DODH) is a promising approach. We report the catalytic DODH of substrates from renewable resources using Cp*ReO3 (1 mol%, TON up to 32) as a catalyst and hydrogen as a reductant. Additionally, the synthesis of Re(V)-diolates is described for tartrates, glycerol and erythritol derivatives. These Re(V)-diolates were obtained in 35 – 53% yield and as mixtures of diastereomers. Their characterization was accomplished using 2D NMR, MS, IR and X-ray diffraction. A NOESY study revealed an intermolecular exchange of glycerol moieties for the diolate complex. Further, alkene extrusion experiments allowed us to establish a reactivity order for a variety of substituents. The olefin extrusion was the fastest for tartrate units and the slowest for glycerol and erythritol diolates
Triggered contraction of self-assembled micron-scale DNA nanotube rings [Research Data]
No full textContractile rings formed from cytoskeletal filaments mediate the division of cells. Ring formation is induced by specific crosslinkers, while contraction is typically associated with motor protein activity. Here, we engineer DNA nanotubes as mimics of cytoskeletal filaments and a synthetic crosslinker based on a peptide-functionalized starPEG construct. The crosslinker induces bundling of ten to hundred individual DNA nanotubes. Importantly, the DNA nanotube bundles curve into closed micron-scale rings in a one-pot self-assembly process yielding several thousand rings per microliter. Coarse-grained molecular dynamics simulations reproduce detailed architectural properties of the DNA rings as observed by electron microscopy. Furthermore, theory and simulations predict DNA ring contraction – without motor proteins – upon increasing attraction or decreasing bending rigidity of the DNA nanotubes, yielding mechanistic insights into the parameter space relevant for efficient nanotube sliding. We experimentally realize a variation of these parameters by addition of molecular crowders or temperature increase, respectively. In agreement between simulation and experiment, we obtain ring contraction to less than half of the initial ring diameter. DNA-based contractile rings could be a future element of an artificial division machinery in synthetic cells or of contractile muscle-like materials
Two-Photon Microprinting of 3D Emissive Structures Using Tetraazaperylene-Derived Fluorophores [data]
No full textThe application of a new class of fluorophores based on a twisted tetraazaperylene core in the fabrication of emissive organic 3D microstructures has been investigated. The synthesis of a series of tetraazaperyleneamines and their corresponding octaazaperopyrenedioxides (OAPPDOs) with different N-benzyl substituents is reported and their formulation in a photoresist for two-photon laser printing is tested. While the direct incorporation of synthesized tetraazaperylene derivatives in a commercially available resist proved to be insufficient due to insolubility, a homogeneous formulation of the fluorophores using pentaerythritol triacrylate (PETA) as a crosslinker and phenyl-bis-(2,4,6-trimethylbenzoyl)-phosphine oxide (BAPO) as a photoinitator could be achieved. In particular, no significant inhibition of the photopolymerization and therefore, of the printing process has been detected in the presence of the fluorophore OAPPDO derivates in the optimized formulations. Various complex 3D microstructures were successfully printed and characterized using confocal fluorescence microscopy. Importantly, it is shown that tetraazaperylene fluorophores functionalized with 3,4,5-trimethoxybenzyl N-substituents were successfully embedded and distributed homogeneously in the 3D microstructures
Transcriptional pathways of terminal differentiation in high- and low-density blood granulocytes in sepsis [research data]
No full textBackground - Trauma and infection induce emergency granulopoiesis. Counts of immature granulocytes and transcriptional pathways of terminal granulocytic differentiation in blood are elevated in sepsis but correlate with disease severity. This limits their performance as sepsis biomarkers in critically ill patients. We hypothesized that activation of these pathways in sepsis is attributable to immature low-density (LD) rather than mature high-density (HD) granulocytes.
Methods - We included patients with sepsis and systemic inflammatory response syndrome (SIRS) of comparable disease severity, and additionally septic shock, on intensive or intermediate care unit admission. Blood granulocyte isolation by CD15 MicroBeads was followed by density-gradient centrifugation. Flow cytometry was used to determine counts of developmental stages in blood and their relative abundancies in total, HD, and LD granulocytes. Five degranulation markers were quantified in plasma by multiplex immunoassays. A set of 135 genes mapping granulocyte differentiation was assayed by QuantiGeneTM Plex. CEACAM4, PLAC8, and CD63 were analyzed by qRT-PCR. Nonparametric statistical tests were applied.
Results - Precursor counts appeared higher in sepsis than SIRS but did not correlate with disease severity for early immature and mature granulocytes. Precursor subpopulations were enriched at least ten-fold in LD over HD granulocytes without sepsis-SIRS differences. Degranulation markers in blood were comparable in sepsis and SIRS. Higher expression of early developmental genes in sepsis than SIRS was more pronounced in LD and less in HD than total granulocytes. Only the cell membrane proteins CXCR2 and CEACAM4 were more highly expressed in SIRS than sepsis. By qRT-PCR, the azurophilic granule genes CD63 and PLAC8 showed higher sepsis than SIRS levels in LD granulocytes and PLAC8 also in total granulocytes where its discriminatory performance resembled C-reactive protein (CRP).
Conclusions - Transcriptional programs of early terminal granulocytic differentiation distinguish sepsis from SIRS due to both higher counts of immature granulocytes and elevated activity of early developmental genes in sepsis. The sustained activity of PLAC8 in mature granulocytes likely accounts for its selection in the whole blood SeptiCyteTM LAB test. Total granulocyte PLAC8 rivals CRP as sepsis biomarker. However, infection-specific transcriptional pathways, that differentiate sepsis from sterile stress-induced granulocytosis more reliably than CRP, remain to be identified
ALL POCUS TB study
No full textStudy Protocol and Statistical Analysis Plan for ALL POCUS TB Study
---
ALL POCUS TB is a prospective, cross-sectional multicentre cohort study in which the accuracy and the diagnostic yield of Tuberculosis-focused point-of-care ultrasound (POCUS) and image interpretation with artificial intelligence (AI) will be assessed in inpatients and outpatients with presumed TB disease in German and Indian sites. Dedicated POCUS protocols for the diagnosis of HIV-associated extra-pulmonary tuberculosis (EPTB) in high incidence settings have been proposed, but reliable accuracy data for both patients with and without HIV are thus far limited. Another arising ultrasound field with great potential is lung ultrasound (LUS) for the diagnosis and the assessment of disease extent for pulmonary TB. Our study ultrasound protocol will cover both abdominal and thoracic locations in both HIV(+) and HIV(-) patients. In addition, we will follow up patients with confirmed TB under anti-mycobacterial therapy to document the natural evolution of TB-associated sonographic findings and assess their value for monitoring of therapy success. With this study we want to fill this gap and to guide implementation efforts by better characterizing the sensitivity and specificity of sonographic findings for TB diagnosis against a comprehensive reference standard. POCUS has the potential to increase TB case finding and avoid over-diagnosis.
A study on the accuracy of point-of-care ultrasound for the diagnosis of thoracic and abdominal TB. We investigate the accuracy of both single tuberculosis associated findings as well as composite findings such as combinations of pathological changes. We are enrolling patients with presumed TB regardless of HIV-status and will perform subgroup analysis for patients with/without TB and patients with/without diabetes mellitus as possible risk factors. The aim is the description of possible combinations for a point-of-care protocol, which can be used as a non-sputum based triage test for TB. In addition, we evaluate patients longitudinally with ultrasound to determine the value for therapy monitoring for pulmonary and extrapulmonary findings
Out-of-pocket Expenses and Time Spent on Clinic Visits Among HIV Pre-exposure Prophylaxis Users and Other Clinic Attendees in Eswatini
No full textUser costs constitute a barrier to the uptake of HIV pre-exposure prophylaxis (PrEP), but their magnitude appears rarely assessed. In this prospective observational study, we assessed self-reported out-of-pocket expenses (OOPE) and time spent on clinic visits during a PrEP demonstration project in Eswatini. At six public primary care clinics, 240 PrEP users and other clinic attendees were interviewed after a clinic visit. Among the 79.2% of clinic attendees reporting any medical OOPE (e.g., expenses for consultations or drugs) and/or non-medical OOPE (e.g., expenses for transport, food, or phone use), the median total OOPE was 7.54 (IQR 5.42-11) when valuing time spent on a clinic visit with Eswatini's per-capita gross domestic product. Our findings can guide measures to reduce the user costs of PrEP in Eswatini and other contexts in which oral PrEP is provided through health care facilities
Die Münchner Residenz als kommentierte 3D-Rekonstruktion [Data]
No full textDie textbasierten Forschungsdaten zu der Dissertation: Die Münchner Residenz als kommentierte 3D-Rekonstruktion bestehen aus Transkriptionen, die zu umfangreich für eine gedruckte Veröffentlichung waren.
Es handelt sich dabei um die Transkription der Archivalien: BHStA, FS 287a, 1491/92; BHStA, FS 287a, 1494/95: Ausgaben des Münchner herzoglichen Rentmaisters Matthäus Prätzel mit Anmerkungen und dem Versuch einer Zuordnung zur Neuveste und zum Alten Hof in München.
Eine Transkription der Archivalie: BHStA, Fasz. 22, Nr. 52: Inventar der Neuveste von 1513 und 1518 mit dem Versuch einer tabellarischen Zusammenführung.
Die umfangreichste Transkription beinhaltet sämtliche Einträge der Archivalien: BHStA, HBAR, HR II, Fasz 1 u 12: Hofbauamtsrechnungen des Münchner Hofbauamts: 1580, 1581, 1587, 1588, 1589, 1590, 1591, 1592, 1593, 1594, 1600, 1611, 1612, 1613, 1614.
Aufgrund des Rechnungscharakters dieser Quellen wurde die Transkription direkt in einem Tabellenkalkulationsprogramm vorgenommen und in zwei Tabellen unterteilt. Eine Tabelle für die Wochenbeschreibungen und eine weitere Tabelle für die Handwerkerbeschreibung.</p