Theory and practice of meat processing (E-Journal) / Теория и практика переработки мяса
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The influence of heat treatment type on the fat component and heterocyclic aromatic amines formation in minced poultry meat products
Get PDFIn this study the influence of heat treatment type on the change in the fatty acid composition, indices and parameters of fat oxidation, the level of heterocyclic amines formation in the chopped poultry ready-to-eat products enriched with ω‑3 fatty acids and an antioxidant complex were studied. The composition of ingredients and recipes of ready-to-eat products are developed with consideration of the medical and biological requirements for the diets of oncological patients. These ready-to-eat products feature some antioxidant substances in their composition that can bind free radicals, and provide for a reduction in the risk of carcinogens formation during the manufacturing process. The heat treatment was run in several ways, also called as modes — cooking in a microwave oven (MW), cooking in a convection oven in the “convection” mode with preliminary short-term roasting, steam cooking. For comparison, the conventional, i. e. not enriched food sample was used as a control one. The introduction of ω‑3 fatty acids into the food formulation made it possible to change the fatty acid composition towards increasing the proportion of polyunsaturated fatty acids (PUFAs). It was found that the profile of fatty acids was influenced by both the ingredients of the product and the type of its heat treatment. The joint use of a PUFA source and a complex of antioxidants made it possible to obtain a ready-to-eat product with a high level of ω‑3; and to ensure the ratio of ω‑6 group acids: ω‑3 ratio amounts to 1–2:1. Heat treatment of enriched semi-finished products by microwave cooking and by steam cooking showed a lesser effect on the change in the content and composition of polyunsaturated fatty acids — the loss of fatty acids was 1.2% and 2.8%, respectively, while in the “convection” cooking mode with preliminary roasting this loss was equal to 3.5%. It was found that the antioxidant complex in the composition of the food product and gentle heat treatment methods cause less lipid peroxidation and the formation of carcinogenic heterocyclic aromatic amines (HAA) during the food manufacturing process
Evaluation of physical characteristics of chevon as affected by post-mortem carcass dressing and freezing preservation
Get PDFThis study was conducted to investigate the effects of post-mortem dressing methods and freezing preservation on the physical characteristics of chevon. Twenty-seven Red-Sokoto male goats between 10 and 12 months of age weighing 18–20 kg were purchased, stabilized and slaughtered. The carcasses were randomly allotted to three post-mortem dressing procedures (scalding, skinning and singeing) and meat from thigh cuts was frozen for 0, 7, 14 and 21 days. Meat samples were excised each day after thawing for physical analysis and data collected were subjected to analysis of variance (ANOVA) in a completely randomized design experiment with 3x4 factorial arrangement. The significant means were separated with the Duncan multiple range test at p<0.05. The results showed that the post-mortem dressing methods and freezing affected Red-Sokoto chevon significantly with the singeing method exerting the highest detrimental effects on physical attributes of meat with the exception of color, yield and pH, while the skinning method exerted the least detrimental effects. Also, cold, cooking and drip losses as well as thermal shortening, cold shortening and pH values increased between the 14th and 21st day, while color, yield, water holding capacity, texture and shear force values decreased across the three treatments during freezing periods. The effects were more significant in singed and scalded meat than in skinned chevon. It was recommended, therefore, that skinning method be encouraged if meat from Red-sokoto male goats is to be frozen and the period of freezing be limited to 14 days for wholesome meat
Water-holding and water-holding capacity of meat and methods of its determination
Get PDFThe considered topic is of great interest for researchers and practitioners engaged in the development of technology and the production of meat food. In the review, the authors focus on theoretical aspects of the meat capacity to bind and hold water. The characteristic of the forms and strength of the water-to-meat bonds is given. The influence of the structural elements of muscle tissue on the meat capacity to bind and hold water is shown. The different opinions of the specialists are given in regard to the terms “water-binding” (WBC) and “water-holding” (WHC) capacity of meat, and the authors of this research expressed their own opinion. Basing on the analysis of publications, a characteristic is given to forms of water-to-meat bonds strength from the point of view of technological practice: there is tightly bound water, loosely bound available water (immobilized), and loosely bound excessive water (free). The article summarizes the material on the methods of determination of water-holding (water-binding) capacity of meat. It is shown that up to date there is no unequivocal answer about the choice of WHC determination method. To define WHC, it is recommended to subtract the WBC value obtained by one of the gravimetric methods from the value of the indicator obtained by one of the methods of external pressure. This problem requires further research, discussion of issues and methods for determination of water-holding capacity.The considered topic is of great interest for researchers and practitioners engaged in the development of technology and the production of meat food. In the review, the authors focus on theoretical aspects of the meat capacity to bind and hold water. The characteristic of the forms and strength of the water-to-meat bonds is given. The influence of the structural elements of muscle tissue on the meat capacity to bind and hold water is shown. The different opinions of the specialists are given in regard to the terms “water-binding” (WBC) and “water-holding” (WHC) capacity of meat, and the authors of this research expressed their own opinion. Basing on the analysis of publications, a characteristic is given to forms of water-to-meat bonds strength from the point of view of technological practice: there is tightly bound water, loosely bound available water (immobilized), and loosely bound excessive water (free). The article summarizes the material on the methods of determination of water-holding (water-binding) capacity of meat. It is shown that up to date there is no unequivocal answer about the choice of WHC determination method. To define WHC, it is recommended to subtract the WBC value obtained by one of the gravimetric methods from the value of the indicator obtained by one of the methods of external pressure. This problem requires further research, discussion of issues and methods for determination of water-holding capacity
Comparative assessment of beef characteristics from young bulls of different breeds and the influence of storage conditions on meat quality indicators
Get PDFComparative assessment results for quality indicators of meat samples obtained from Black Pied, Simmental and Aberdeen Angus young bulls and the influence of different temperature conditions on the quality of vacuum-packed beef during refrigerated storage are presented. The general chemical composition, physicochemical properties, and biological value of the samples based on the content of nonessential and essential amino acids were determined, as well as the protein quality index (PQI) and amino acid score. Analysis of the general chemical composition revealed higher moisture and protein content and the lowest fat content in Black Pied beef compared to other breeds. The amino acid composition of the protein showed a higher content of essential amino acids and the highest PQI value in Simmental beef. According to the calculation results, higher amino acid scores for lysine (149.1% and 129.1%) and tryptophane (200.0% and 240.0%) were noted in meat from Simmental and Aberdeen Angus young bulls, respectively. For the process of storing vacuum-packed meat in a cooled (at a temperature of 2.0 ± 0.5 °C) and superchilled state (minus 2.0 ± 0.5 °C) at a relative air humidity of 85%, a comparative analysis of changes in free amino acids and dynamics of hydrolytic and oxidative spoil- age of meat samples from various breeds was conducted. After 21 days of storage in a superchilled state, the content of free amino acids in Black Pied, Simmental and Aberdeen Angus beef was lower by 13.1% (P > 0.05), 24.1% (P ≤ 0.05) and 17.0% (P ≤ 0.01) compared to storage in a cooled state, respectively. For all studied samples stored in a cooled state, the acid number values were 40% to 41% (P ≤ 0.01) higher than in a superchilled state and peroxide number values were 20% to 23% (P ≤ 0.05) higher than in a superchilled state. It has been established that lowering the storage temperature of vacuum-packed beef helps to better preserve quality and ensure safety for meat from all breeds studied
Hydrolysate of ovalbumin: production and evaluation of the functional properties of peptides
Get PDFChicken eggs proteins and their derivatives, like protein hydrolysates, peptides and amino acids, possess high nutritional value and provide a wide range of biological activity. They serve as sources for development of functional ingredients that draw the attention of specialists in the food production and biomedical industries, as well as the livestock feed industry. Enzymatic hydrolysis of proteins is a popular process for obtaining bioactive peptides with multifunctional properties. The purpose of this study is to obtain a hydrolysate of ovalbumin with a high degree of hydrolysis and to determine its functional and technological parameters. The research presents a two-stage scheme of ovalbumin hydrolysis with the help of pepsin and trypsin which provide high degree of hydrolysis (82–83%). The fractional composition of the hydrolysate is determined. The fractional composition is represented by three main fractions (high, medium and low molecular weight). The summarized antioxidant activity (SAA) of the hydrolysate is considered within the dynamics of the hydrolysis process. The highest SAA value was noted after 2 hours, and it amounted to 170.23 mg/l; at the end of hydrolysis the SAA value was equal to 114.31 mg/l. When analyzing the SAA, it was found that the main contribution to the summarized antioxidant activity of the ovalbumin hydrolysate is made by peptides of the medium molecular fraction. The microfiltration process, used in the research, made it possible to separate high-molecular compounds, which led to an increase in the SAA of the hydrolysate to 189.9 mg/l. The main functional and technological parameters of the hydrolysate are determined in this research. The comprehensive study of the biological activity and functional characteristics of egg protein hydrolysates and their peptides provides a theoretical basis for expanding the range of functional ingredients obtained from food proteins and for replenishing the range of functional foods.Chicken eggs proteins and their derivatives, like protein hydrolysates, peptides and amino acids, possess high nutritional value and provide a wide range of biological activity. They serve as sources for development of functional ingredients that draw the attention of specialists in the food production and biomedical industries, as well as the livestock feed industry. Enzymatic hydrolysis of proteins is a popular process for obtaining bioactive peptides with multifunctional properties. The purpose of this study is to obtain a hydrolysate of ovalbumin with a high degree of hydrolysis and to determine its functional and technological parameters. The research presents a two-stage scheme of ovalbumin hydrolysis with the help of pepsin and trypsin which provide high degree of hydrolysis (82–83%). The fractional composition of the hydrolysate is determined. The fractional composition is represented by three main fractions (high, medium and low molecular weight). The summarized antioxidant activity (SAA) of the hydrolysate is considered within the dynamics of the hydrolysis process. The highest SAA value was noted after 2 hours, and it amounted to 170.23 mg/l; at the end of hydrolysis the SAA value was equal to 114.31 mg/l. When analyzing the SAA, it was found that the main contribution to the summarized antioxidant activity of the ovalbumin hydrolysate is made by peptides of the medium molecular fraction. The microfiltration process, used in the research, made it possible to separate high-molecular compounds, which led to an increase in the SAA of the hydrolysate to 189.9 mg/l. The main functional and technological parameters of the hydrolysate are determined in this research. The comprehensive study of the biological activity and functional characteristics of egg protein hydrolysates and their peptides provides a theoretical basis for expanding the range of functional ingredients obtained from food proteins and for replenishing the range of functional foods
Investigation of the chemical composition, physicochemical properties, and microstructure of meat patties with amaranth flour
Get PDFThis study aimed to investigate the effect of adding amaranth flour to meat patties on their chemical composition. Four different variations of meat patties were prepared, with amaranth flour added at concentrations of 5%, 10%, and 15% in place of beef. The control sample was prepared without any addition. The results of the study showed that the addition of amaranth flour led to a significant decrease in the moisture content of the meat patties, while the proportions of carbohydrates, fat, and ash increased. Specifically, the patties with the highest concentration of amaranth flour (15%) had the highest proportions of carbohydrates and fat with the lowest proportion of moisture. The control sample had the highest moisture content and the lowest proportion of carbohydrates, fat, and ash. The addition of amaranth flour increased the water-holding capacity of the meat patties, with the highest increase observed in the sample with 15% amaranth flour (82.21%). The overall score of sensory evaluation of the meat patties did not significantly decrease with the addition of up to 10% amaranth flour, according to the sensory evaluation. The study provides evidence that up to 10% amaranth flour can be used as a substitute for beef in meat patties, which can lead to an increase in the fat and carbohydrate content and mineral composition and improvement of the water-holding capacity of the final product
Technological approaches to the extraction and purification by ultrafiltration techniques of target protein molecules from animal tissues: a review
Get PDFEffective isolation and purification of protein is a great challenge nowadays. The key aspect is protein stability and solubility, which primarily depend on protein structure and its amino acid sequence. Manipulations with pH and ionic strength are the first at tempts to increase protein stability and solubility. Different additives that are allowed or prohibited in the food industry are applied for overcoming protein aggregation. Sugars, polyhydric alcohols and amino acids are the most attractive among them. Trehalose, glycerol, arginine, glycine and proline demonstrated outstanding properties that make them perspective for application during iso lation and purification of proteins singly or in combination with each other or othercompounds. However, the algorithm of effective isolation and purification of protein could be significantly varied depending on its structure. Effective isolation and purification of protein is a great challenge nowadays. The key aspect is protein stability and solubility, which primarily depend on protein structure and its amino acid sequence. Manipulations with pH and ionic strength are the first at tempts to increase protein stability and solubility. Different additives that are allowed or prohibited in the food industry are applied for overcoming protein aggregation. Sugars, polyhydric alcohols and amino acids are the most attractive among them. Trehalose, glycerol, arginine, glycine and proline demonstrated outstanding properties that make them perspective for application during iso lation and purification of proteins singly or in combination with each other or othercompounds. However, the algorithm of effective isolation and purification of protein could be significantly varied depending on its structure
N-Ethylmaleimide influenced the evaluation of disulfide cross-links in the oxidized myofibrillar proteins using the non-reducing SDS-PAGE
Get PDFThe present study aimed to investigate the effect of N-Ethylmaleimide (NEM) on the evaluation of disulfide formation in the oxi dized myofibrillar proteins during the sample preparation of the non-reducing SDS-PAGE procedure. For this purpose, extracted myofibrillar proteins were oxidized firstly via a Fenton oxidation reaction, and non-oxidized proteins were used as a control. Before running SDS-PAGE, in the sample preparation, these oxidized and non-oxidized proteins were prepared according to the three dif ferent sample preparation methods with or without the presence of N-Ethylmaleimide or β-mercaptoethanol. Results showed that oxidized proteins treated with NEM regardless of sample preparation methods presented attenuated bands of myosin heavy chain monomer in the non-reducing SDS-PAGE gels, suggesting that the disulfide bonds formed as a result of protein oxidation could be preserved by NEM during sample preparation. Meanwhile, a possible mechanism for the effect of NEM was proposed.The present study aimed to investigate the effect of N-Ethylmaleimide (NEM) on the evaluation of disulfide formation in the oxi dized myofibrillar proteins during the sample preparation of the non-reducing SDS-PAGE procedure. For this purpose, extracted myofibrillar proteins were oxidized firstly via a Fenton oxidation reaction, and non-oxidized proteins were used as a control. Before running SDS-PAGE, in the sample preparation, these oxidized and non-oxidized proteins were prepared according to the three dif ferent sample preparation methods with or without the presence of N-Ethylmaleimide or β-mercaptoethanol. Results showed that oxidized proteins treated with NEM regardless of sample preparation methods presented attenuated bands of myosin heavy chain monomer in the non-reducing SDS-PAGE gels, suggesting that the disulfide bonds formed as a result of protein oxidation could be preserved by NEM during sample preparation. Meanwhile, a possible mechanism for the effect of NEM was proposed
Dioxins and dioxin-like compounds in meat and meat products
Get PDFDioxin and dioxin-like compounds are persistent organic pollutants that received considerable attention in recent years due to their high potential toxicity, wide distribution and extreme stability. Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) mainly occur in the environment as a result of several human activities including combustion, incineration and many other industrial activities, whereas polychlorinated biphenyls (PCBs) congeners were intentionally manufactured and widely used in various fields. Since dioxin and dioxin-like compounds are found in various environmental compartments (air, water, soil, sludge, sediment, food, feed, blood, animal and human tissues), humans could be exposed to them via inhalation, dermal contact or food ingestion. However, 90% of human exposure to dioxin is through food ingestion particularly foods from animals and foods that are rich in fat. In contrast, only low levels have been found in food items of plant origin. Exposure to dioxin compounds is associated with various adverse health problems. However, their toxicity varies dramatically according to the type of dioxin, species of exposed organism, as well as exposure frequency and duration. Dioxins are mainly determined by instrumental chromatographic methods such as GC-HRMS and GC–MS/MS. Many efforts have been made to remove, reduce and prevent these hazardous substances from the environment. However, the best method for reducing human exposure to dioxins and dioxin-like compounds is controlling and minimizing their production. In this article, structures, sources, exposure, toxicity and analysis methods of dioxin and dioxin-like compounds in meat and other foods were reviewed.Dioxin and dioxin-like compounds are persistent organic pollutants that received considerable attention in recent years due to their high potential toxicity, wide distribution and extreme stability. Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) mainly occur in the environment as a result of several human activities including combustion, incineration and many other industrial activities, whereas polychlorinated biphenyls (PCBs) congeners were intentionally manufactured and widely used in various fields. Since dioxin and dioxin-like compounds are found in various environmental compartments (air, water, soil, sludge, sediment, food, feed, blood, animal and human tissues), humans could be exposed to them via inhalation, dermal contact or food ingestion. However, 90% of human exposure to dioxin is through food ingestion particularly foods from animals and foods that are rich in fat. In contrast, only low levels have been found in food items of plant origin. Exposure to dioxin compounds is associated with various adverse health problems. However, their toxicity varies dramatically according to the type of dioxin, species of exposed organism, as well as exposure frequency and duration. Dioxins are mainly determined by instrumental chromatographic methods such as GC-HRMS and GC–MS/MS. Many efforts have been made to remove, reduce and prevent these hazardous substances from the environment. However, the best method for reducing human exposure to dioxins and dioxin-like compounds is controlling and minimizing their production. In this article, structures, sources, exposure, toxicity and analysis methods of dioxin and dioxin-like compounds in meat and other foods were reviewed
Trophological approach in the development of nutrition theories
Get PDFThis article presents a trophological approach to the development of nutrition. The evolution of human views on nutrition from antiquity to the present time, including issues of perfect nutrition, is considered. The main concepts of nutrition theories are formulated. The systematized reasoning is useful for new rationale for solutions of challenges related to optimization of nutrition. Within the scope of the development of nutrition theories, the problem of perfect food plays a special role. The article presents a modern vision of perfect nutrition. The designing of trophological nutrition algorithm is based on scientific approaches that both to justify the correct consumption of food products, and also define the most reasonable way of their production, storage, distribution and disposal of food waste. The implementation of the trophological approach in theories of nutrition is also reflected in the concept of the state policy of the Russian Federation in the field of healthy nutrition among the population. This topic is also relevant in light of the sharp increase of food allergies among the various groups of population, because people who suffer from food allergies must follow a special diet to prevent the risk of allergic reactions, which can be managed using a nutritional approach