Atom Indonesia (E-Journal)
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    The Effect of Gamma-Irradiated Nitrate-Reducing Bacteria in Decreasing the In Vitro Production of Methane by Buffalo Rumen Liquid

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    Ruminant livestock undeniably contributes to the increase of greenhouse gases by emitting methane. One strategy used to reduce methane emission is by applying nitrate-reducing bacteria (NRB). Utilizing active NRB has a side effect of acidosis in rumen liquid in an uncontrolled condition. Alternatively, NRB can be applied in an inactive form. The aim of this study was to examine the use of gamma irradiation on NRB to be used for decreasing methane production by buffalo rumen liquid. The examination was performed in vitro and involved comparison to other treatments, which were active NRB, autoclaved NRB, and without NRB. The result showed that the NRB in either active or the inactive conditionaffected fermentation of the rumen microorganisms. There were differences in pH values, digestibility of organic matters, ammonia, total and partial volatile fatty acids, and methane production between all treatments after 24 and 48 h of incubation. All treatments showed significant differences for each parameter, except for the gas production (p ≤ 0.05). The irradiated NRB produced slightly more methane, i.e. 3.0 and 10.4 ml/200 mg after 24 and 48 h incubation, respectively, compared to the active NRB, autoclaved NRB, and no-NRB-addition treatments, i.e., 2.0 and 8.0; 7.8 and 11.7; 8.0 and 12.4 ml/200 mg, respectively. In conclusion, the irradiated NRB has a potency as a supplement feed for buffaloes to reduce methane production without the risk of acidosis in the rumen liquid

    Expression of γ-H2AX, 53BP1 and Micronuclei as Genome Damage Biomarker of Population in Keang and Salumati Village, Mamuju West Sulawesi Province

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    The residents living in high background radiation area have risk to be exposed by ionizing radiation that also potentially cause their DNA damage. The aim of this study was to determine the expression of γ-H2AX, 53BP1 foci and micronuclei in the residentswho live in high background radiation area of Salumati village, Mamuju, West Sulawesi, Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of γ-H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas micronuclei was detected after being cultured and giemsa stainedaccording to standard procedures. Results showed that both γ-H2AX and 53BP1foci from high background area was lower than that of control area (0.37±0.24vs 0.19±0.11 (p=0.03) for γ-H2AX and 0.61±0.30vs 0.31±0.12 for 53BP1 (p=0.01)). The mean of micronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0.02 (0.003-0.02). There was statistical significant in corellation between both γ-H2AX, 53BP1 foci with micronuclei index in exposed area (p=0.02, p=0,04 respectively).In conclusion,there was a positive correlation between γ-H2AX and 53BP1 foci to micronuclei and this might be a clue of the occurrence of genome repairing mechanism caused by natural radiation at low dose chronical exposure in thestudied area

    Current Status and Recent Achievements of the Sterile Insect Technique Program Against Dengue Vector, Aedes aegypti, in Indonesia

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    Given the facts that Indonesia has suitable environment condition to survival, reproduction and development of the principal mosquito vector for dengue virus, Aedes aegypti, the transmission of dengue virus does routinely occur every year. The sterile insect technique (SIT) as one of the techniques in area-wide integrated pest management (AW-IPM) has been developed to control Aedes aegypti population since 2004. The first 5 years of the program (2004-2009) was focused on mass rearing facility establishment. The lab-work study for the entomological data and field trials were performed in 2010-2014. In 2014-2019, the program was addressed to enhance the mass rearing facility to increase sterile male production and strengthening the networking throughout the involvement in the regional and interregional program coordinated by International Atomic Energy Agency (IAEA). The main future challenge of the SIT program against Aedes aegypti is developing the capacity building and strong commitment of all stakeholders i.e. the Ministry of Health, the universities, the research institutes and the public. In the future work plan, the pilot project design to proof the concept of the SIT is strongly recommended to obtain a firm, unbiased and strong results as the consideration for the decision maker to create the Aedes control program in Indonesia

    A Bayesian Network Approach to Estimating Software Reliability of RSG-GAS Reactor Protection System

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    Reliability represents one of the most important attributes of software quality. Assessing the reliability of software embedded in the safety of highlycritical systems is essential. Unfortunately, there are many factors influencing software reliability that cannot be measured directly. Furthermore, the existing models and approaches for assessing software reliability have assumptions and limitations which are not directly acceptable for all systems, such as reactor protection systems. This paper presents the result of a study which aims to conduct quantitative assessment of the software reliability at the reactor protection system (RPS) of RSG-GAS based on software development life cycle. A Bayesian network (BN) is applied in this research and used to predict the software defect in the operation which represents the software reliability. The availability of operation failure data, characteristics of the RPS components and their operation features, prior knowledge on the software development and system reliability, as well as relevant finding from references were considered in the assessment and the construction of nodes on causal network model. The structure of causal model consists of eight nodes including design quality, problem complexity, and defect inserted in the software. The calculation result using Agenarisk software revealed that software defect in the operation of RPS follows binomial statistic distribution with the mean of 1.393. This number indicated the high software maturity level and high capability of the organization. The improvement of software defect concentration range on the posterior distribution compared with the prior’s is also identified. The result achieved is valuable for furtherreliability estimation by introducingnew evidence and experience data, and by setting up an appropriate plan in order to enhance software reliability in the RPS

    Preface Atom Indonesia Vol 45 No 3

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    Acknowledgement Atom Indonesia Vol 45 No 3

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    Cover Atom Indonesia Vol 45 No 3

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    Acknowledgement Atom Indonesia Vol 45 No 2

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    Investigation on the Performance of a Wickless-Heat Pipe Using Graphene Nanofluid for Passive Cooling System

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    To enhance the thermal safety in case of station blackout, a wickless-heat pipe is proposed as an alternative passive cooling system technology to remove decay heat generation in the nuclear spent fuel storage pool. The objectives of this research are to investigate the heat transfer phenomena in vertical straight wickless-heat pipe using Graphene nanofluid working fluid and to study the effect of Graphene nanofluid on the vertical straight wickless-heat pipe thermal performance. The investigation was conducted in 6 meters height and 0.1016 m inside diameter of vertical straight wickless-heat pipe. In this research, the Graphene nanofluid with 1 % of weight concentration was used as working fluid. The effect of working fluid filling ratio, evaporator heat load, and coolant volumetric flow rate on the water jacket were studied. The results showed that the heat transfer phenomena, which were indicated by an overshoot, zigzag, and stable state, were observed. Based on thermal resistance obtained, it was shown that the vertical straight wickless-heat pipe charged with the Graphene nanofluid has a lower thermal resistance compared to one with demineralized water. The thermal resistance of vertical straight wickless-heat pipe using Graphene nanofluid and demineralized water were 0.015 °C/W and 0.016 °C/W, respectively. While the best thermal performance was achieved at a filing ratio of 80 %, higher heat load, and higher coolant volumetric flow rate. It can be concluded that Graphene nanofluid could enhance the thermal performance of vertical straight wickless-heat pipe

    The Absolute Standardization Methods of 32P for Calibrate Nuclear Medicine Instruments in Indonesia

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    The absolute standardization of 32P radioactive sources employedto calibrate nuclear medicine instruments has been conductedat PTKMR-BATAN. We deemed this activity to be necessarysince 32P used in the nuclear medicine fields has a short half-life, and in order to obtain a result of quality measurement, it requires a special treatment. Moreover, in Indonesia, the use of nuclear medicine techniques has developed rapidly. We prepared all the radioactive sources witha gravimetric method by using a KERN ABT 220-5DMsemi-micro type scale, traceable to the International Unit System. We conductedthe 32P standardization by employing a 4πβ(PS)-γ coincidence method with 60Co as a tracer;meanwhile, we conducted the impurity measurementby employing a beta spectrometer system. The result of 32P absolute measurement was 380.05 Bq/mg with a 0.68-percentrange uncertainty, witha k=2coverage factor. This value was used to calibrate a “Capintec CRC-7BT”dose calibrator that was a secondary standard instrument in PTKMR-BATAN. The results showed us that the calibration factor of the “Capintec CRC-7BT” dose calibrator was 1.12 witha 4.7-percent uncertainty

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