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Data Management Plus: Organizing Data and Program Files for a University-Community Partnership
Get PDFPoster presented at International Association for Social Science Information Service and Technology (IASSIST) Annual Conference, Halifax, Canada. May 2024
Complexation of Diserinol Isophthalamide with Phosphorylated Biomolecules in Electrospray Ionization Mass Spectrometry
Get PDFElectrospray ionization (ESI) enables gentle transfer of biomolecules from solution to vacuum, facilitating the study of biomolecular structure under highly controlled conditions. However, biomolecules are desolvated during the ESI process, and the loss of ionic hydrogen bonds to solvent molecules can drive structural rearrangement, most prominently at solvent-exposed charge sites. Microsolvation reagents can bind to these bare charge sites in ESI mass spectrometry (ESI–MS) experiments, providing alternative intermolecular interaction partners. Previously, 18-crown-6 was shown to be an effective reagent for binding to cationic monoalkylammonium residues. More recently, diserinol isophthalamide (DIP) was reported as an analogous anionic microsolvation reagent, primarily for carboxylate residues of small model peptides. Herein, we expand upon this work to examine the complexation of DIP, 1,1’-(1,2-phenylene)bis(3-phenylurea) (PBP), and triclocarban (TCC) with molecules featuring a terminal or linking phosphate moiety. Specifically, using ESI–MS, we assess the binding of these reagents with dimethyl phosphate (DMP), cyclic adenosine monophosphate (cAMP), dibutyryl cAMP, RNA dinucleotides ApU and CpG, and angiotensin II phosphate (DRVpYIHPF). For DMP, the smallest target molecule, reagents TCC, PBP and DIP showed favorable adduction. However, for larger systems, PBP and TCC showed reduced complexation, which was attributed to steric hindrance from the terminal aromatic moieties of PBP and the limited hydrogen bonding network of TCC. Overall, of the three reagents, DIP showed the most consistent performance for anionic microsolvation of phosphate groups, facilitating future studies of gas-phase biomolecular structure and the effects of microsolvation
Gendered Solidarity and the Shifting Ground of the Black Feminine
No full textCatherine John argues that, in a series of ironies, negative stereotypes of Black female gender identity have laid the foundation for something new: a gender identity that defies containment. This talk situates the “attitude” associated with excessive Black femininity within the contexts of the work of Jamaican scholar Sylvia Wynter, African American scholar Hortense Spillers and Afro-Brazilian scholar Denise Ferreira da Silva. Ferreira da Silva’s notion that Black women “neither comply [with] nor disappear [from]” the hegemonic order will be used to explore the shifting ground between invisibility and a disruptive power that can be deployed for either creative or destructive purposes. Among other things, this presentation will ponder what gendered solidarity looks like with these rebellious new subjects.
John’s talk, which was presented recently at the Modern Language Association conference in January ’24, is in part also explored in an article forthcoming in fall 2024 in Frontiers: A Journal of Women’s Studies, entitled, “Insurgent Attitude and the Shifting Ground of the Black Feminine.” This piece has also been influenced arguments made in the chapter, “ÌYÁ but not Woman: A Grassroots Formulation of the Afrospora Feminine,” from John’s forthcoming manuscript Afroindigenization: Marasa Consciousness in the Afrospora