11603 research outputs found
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Gifts at work: spark wonder campaign
Get PDFSupport from alumni and donors allows the Linfield community to shin
The effect of low concentrations of copper on mitochondria and activity in yeast cells
Get PDFMitochondria are essential organelles in both yeast and human cells due to their role in metabolism, ATP production via the oxidative phosphorylation (OXPHOS) pathway, and other regulatory cellular processes. Using yeast as a model organism to study mitochondrial function in a chronological lifespan assay allows experiments to be conducted over a shorter timeframe and allows for connections to be made to human cells. Our aim is to investigate how exogenous copper in the mitochondria of yeast affects the production of reactive oxygen species (ROS), protein expression, and enzyme activity during yeast lifespan. Small amounts excess copper added to growth media (0.25 mM copper sulfate in restricted nutrient media) extend yeast chronological lifespan, but yeast lifespan is reduced when added copper levels are increased to 2.0 mM copper sulfate or higher. These results indicate that low levels of exogenous copper in the media is beneficial for yeast in these restricted media conditions. To extend these findings, we assessed how added copper changes mitochondria within these cells over the course of the yeast lifespan (14 days growth). Using MitoTracker Green and fluorescence detection we showed an increase in mitochondria in copper treated cells. This is consistent with previous studies showing mitochondria in mammal and yeast cells contain a labile copper pool located in the matrix, which is used in the metalation of the copper containing enzyme of the OXPHOS pathway Complex IV, Cytochrome C Oxidase (CcO), and superoxide dismutase (Sod1p). Our most recent work focuses on assessing CcO protein complex expression during yeast lifespan, specifically looking at cytochrome c oxidase subunits using western blotting; and assessing Sod1p activity using in-gel activity assays. The results of this research allow us to better understand the role of copper in mitochondrial activity across the lifespan of yeast
Analyzing Crossdressing in \u27Adventures of Huckleberry Finn\u27 from a Queer Perspective
No full textLiterature criticism essay
The Increasing Price of Automobiles Through Time
No full textThe automobile has become an integral part of the United States over the last 100 years. Not only is the car a tool that increases the likelihood employment and job retention, but it can also be used as symbol of status and socioeconomic standing. However, with increased safety, efficiency and quality standards, the automobile is quickly becoming a much more expensive item than it was before. This analysis works to find the key factors which affect new vehicle sales in the U.S. and how the automobile market has changed because of those factors
Realized Publicity Design for Eco-Tales
No full textA project focusing on the publicity design for the Linfield Theatre production of Eco-Tales. This poster and presentation highlights the collaborative scholarly and creative processes of theatre students, theatre faculty, and theatre professionals
Does a Drosophila Melanogaster Mutant Exhibit a General Defect in MicroRNA-Mediated Gene Silencing?
No full textMature microRNAs (miRNA) are ~22 nucleotide long single-stranded ribonucleic acids encoded by the genome and essential for gene silencing. Silencing occurs when miRNAs are processed and subsequently associate with the miRNA-induced silencing complex (miRISC). miRISC binds via complementary base pairing to target mRNAs, and target mRNAs are silenced by either mRNA degradation, translational block, or both. microRNA-based therapeutics are being developed and deployed to treat diseases caused by altered gene expression.
We aim to characterize the molecular mechanisms of microRNA-mediated gene silencing. We have Drosophila melanogaster mutant fly lines with disrupted gene silencing as visualized by a GFP-based fluorescent reporter of silencing. We are now curious as to whether the observed change in fluorescence is the result of a disruption to microRNA-mediated gene silencing in general, or if the observed change in fluorescence reflects a disruption of a subset of microRNAs or microRNA targets. We are building fly lines and appropriate controls that will enable us to test for changes in microRNA-mediated gene silencing using a different and vetted reporter of gene silencing that is active in developing neurons. Recent efforts have introduced the nerfin-GFP sensor into our mutant fly line and a control line, setting the stage for comparative analysis of gene silencing
Apple v Google
No full textWe are investigating the stock price of Apple and what variables cause it to vary from year to year. As we look at Apple, we are also looking at consumer preferences and comparing it to the next highest technology competitor, Google. We chose to see whether Apple stock lagged, U.S. household income, investor sentiment, earnings per share, bond options, interest rates, and Steve Jobs or Sundar Pichai as CEO influences stock prices. With these variables, we expect to learn what influences Apple’s and Google’s stock price and how consumer preferences affect stock price. We would be able to look at the changes in the independent variables and understand how that affects the stock prices
This IS my Linfield
Get PDFAs I watched construction crews this summer begin the process of turning Mac Hall into something new and wonderful (see page 8), I was reminded of my own history with that building. Nearly 50 years ago, Linfield was in serious financial trouble. Faculty members were asked to help clean the residence halls in preparation for fall semester, since the cleaning services staff had been severely reduced as part of budget cuts
Changed by the pandemic
Get PDFA resurgent interest in public health and a sharper focus on the mental health of those delivering care are among the lasting transformations resulting from a worldwide crisis