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Editor\u27s Comments
Get PDFIn this issue there are several papers of note. Dr. Pillsbury and Dr. Kromm have had their papers published which were presented at the G.T.U. sessions held in conjunction with the N.C.G.E. and A.A.G. meetings. Three other papers indicate two dimensions of activities. In two there is evidence of considerable individual scholarly work and the continuation of a time honored solitary approach to study. The other is the blending of individual efforts into a common goal-the pursuit of information about a timely topic. All are worthy efforts. Finally, President Speigner has made a call to all members of Gamma Theta Upsilon. We anticipate a fine session for G.T.U. at the Atlanta meeting of N.C.G.E. November 25-27. We hope many members can attend and indicate their desires for G.T.U
Student Engagement in ELA Choice-Based Core Curriculum: A Case Study of the Dramatic Writing for Film, Television, and Theater Course
Get PDFThis paper describes a case study examining student feelings towards engagement in choice-based core ELA courses and teacher feelings towards student engagement specifically within the Dramatic Writing for Film, Television, and Theater course offered in the state of Georgia. The context of this study focuses on the impact that student agency, choice, and voice have on student engagement from their own perspective and how Invitational Education Theory connects to this impact and the course itself. The case study examines the course, its students, and teachers in two schools in a large, suburban metro Atlanta school district. Through interviews and observations, the study found that students enrolled in the course experienced higher levels of engagement when compared to previous, non-choice courses. This study is the first to examine the impact of the Dramatic Writing course since its inception
A Fixed Cell Fungal Catalyst for Production of Melanin in Submerged Fermentation
No full textMelanin is a naturally occurring pigment, that is produced by organisms widely across the biological kingdoms. Eumelanin, the most widely recognized class of melanins, is characterized by its dark brown to black visual appearance along with possession of a plethora of documented properties such as UV resistance, antioxidant activity, and bioremediation potential. These qualities have driven growing interest in its use across different including cosmetics, medicine, and electronics. While eumelanin is traditionally sourced from the common cuttlefish, Sepia officinalis, this process suffers from a limited supply of cuttlefish and raises ethical concerns. As an alternative, the submerged fermentation of microorganisms is commonly used in industry for the production of various goods including antibiotics, enzymes, and organic acids. These processes are incredibly effective and, in many cases, render other methods of production obsolete. Multiple studies have reported successful biosynthesis of eumelanin from microorganisms, underscoring the potential for environmentally friendly and scalable production in this manner. This project utilizes submerged fermentations of the white rot fungus Pleurotus ostreatus for the production of eumelanin and tests the implementation of fixed cell catalyst system to facilitate this process. Additionally, this project aims to address common problems associated with upscaling by investigating and comparing eumelanin production in benchtop shake flasks and in bioreactor controlled vessels with an increased working volume, as well as attempting to improve eumelanin yields through exploration of conditions for fungus P. ostreatus
Our Relentless Objectification of Native Americans: As Exemplified by Louise Erdrich and Cherie Dimaline
Get PDFIndigenous communities relentlessly challenge the normalized commodification of their culture and beliefs. One way they actively address these oppressions is through storytelling. Their accounts educate readers and encourage them to re-frame modern perspectives and how stereotypes affect Native communities. Their stories are not a simple myth; instead, they serve as an active participant to combat actions that treat Indigenous people as an anomaly. We should treat Native Americans as human beings who invariably struggle with present-day degradations, commodification, and continual displacement. Indigenous authors reclaim their narrative by using their historical context and storytelling skills to dissect the parallels between fact and fiction. Two excellent examples of this marriage of reality and fiction are Cherie Dimaline’s The Marrow Thieves and Louise Erdrich’s Future Home of the Living God. Analyzing the treatment of Native peoples in both life and death will help us better understand the historical influences, normalized justifications, and stigmas that surround Indigenous peoples
Incorporating Extensive Reading into the Foreign Language Curriculum
Get PDFExtensive reading is an approach to language learning that encourages learners to read a large quantity of easy reading material. A substantial body of research confirms the effectiveness of this approach for developing proficiency in both first (L1) and second (L2) languages. This paper explains what extensive reading is, why it is important, and how it can be incorporated as part of a language curriculum. The paper also provides a review of research on the effectiveness of extensive reading in several different languages and recommends many extensive reading resources, including open access materials, to facilitate the implementation of extensive reading for teachers and practitioners
John Stagmeier, Violin
Get PDFJohn Stagmeier, Violin
Eric Jenkins, Pianohttps://digitalcommons.kennesaw.edu/musicprograms/2906/thumbnail.jp
Joey Garcia, Saxophone
Get PDFJoey Garcia, Saxophonehttps://digitalcommons.kennesaw.edu/musicprograms/2904/thumbnail.jp
Assessment of real time internalization of cell penetrating peptide cargos with adaptors
No full textCell penetrating peptides are a promising tool for an efficient and effective way to deliver therapeutic cargos to intracellular targets. CPPs have shown the ability to easily permeate cell membranes but are often entrapped in endosomes where they are either recycled out of the cell or digested in lysosomes. The rate of CPP internalization, effect of internalization on target cells, and method of transport across the membrane are poorly understood aspects of CPP delivery. One promising CPP adaptor is TAT-CaM, which consists of HIV transactivator of transcription peptide, the CPP, fused with human calmodulin. Cargo proteins are noncovalently and reversibly linked to TAT-CaM via binding to a calmodulin binding site engineered onto the cargo. During endocytosis and cellular trafficking cargos readily dissociate and efficiently avoid endosomal entrapment. In this study we assess the process of intracellular delivery, endosomal entrapment, and endosomal escape of fluorescent CPP cargos with a variety of adaptors, and how this effects the action of the CPP complex within the cell. We will use confocal microscopy to visualize the process of intracellular delivery of the CPP complex. Assessing this process in real time answers many valuable questions such as cellular responses to CPP internalization, internalization rate, efficiency of cargo dissociation from TAT-CaM and other cargos, rate of endosomal escape in relation to different CPPs, and potential mechanisms of intracellular transport. Answering these questions opens the door to developing more efficient and effective CPP adaptors to deliver therapeutic cargos to intracellular targets
Identification of Lipids in Bovine Brains Using Untargeted Liquid Chromatography and Mass Spectrometry
No full textLipids are a crucial molecule for maintaining healthy brain cells. Imbalances of lipid classes are strongly correlated with certain diseases of the brain, which can extend to the rest of the body. Knowing the general lipid composition of healthy mammalian brains helps to identify changes that are caused by disease and use those markers to help develop more accurate diagnostic tests or even therapeutics for the disease. In this study, the lipid composition of bovine brain was determined by utilizing untargeted Liquid Chromatography-Mass Spectrometry (LCMS). This technique allows for accurate identification of the lipid classes found in a brain sample that has been prepared using Folch extraction. The Folch extraction is performed using a mixture of methanol and chloroform mixed with a brain sample. The mixture is allowed to incubate at room temperature for an hour while being mixed by a shaker to help extract the lipids. Then, water is added to mixture and centrifuged to separate the organic phase and the aqueous phase. The organic phase is transferred to another microcentrifuge tube to evaporate the organic solvent using a vacuum centrifuge. The sample is then reconstituted in methanol: acetonitrile (90:10). Once reconstituted, lipids are separated using a reverse phase C18 column in a Vanquish Flex HPLC, with a 12-minute gradient containing water and acetonitrile. The mass spectrometer is coupled with the LC in positive and negative mode to determine the m/z of the lipids present in the sample. Initially Compound Discover software was used to identify preliminary matches which then further filtered using the LIPIDMAPS software to classify the lipids such as Sphingolipids, Glycerolipids, Fatty acyls, Glycerophospholipids, and Sterol lipids. Preliminary analysis of a bovine brain showed the most prevalent lipid classes to be fatty acyls, by a large margin, followed by Glycerophospholipids