North Carolina Agricultural and Technical State University
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Amplification-by-Polymerization in Volatile Organic Compound Sensing and CRISPR-Integrated Biosensing
No full textBreath-based diagnostics are emerging as effective tools for detecting respiratory diseases, including those caused by coronaviruses. The body produces volatile organic compounds (VOCs), which change in response to different health conditions and act as markers for specific diseases. VOCs impact on surface polymer growth introduces the idea of Amplification-by Polymerization (AbP)-assisted VOC detection. In this method, VOCs influence both DNA hybridization efficiency and polymer chain propagation, offering the detection signal readout in a detector-free fashion. Salmonella is listed by the Centers for Disease Control and Prevention (CDC) as one of the most common foodborne pathogens and it is a bacterium to trigger symptomatic infections while experiencing diarrhea, fever, etc. Genomic DNA from Salmonella as a target analyte can be used for assay development, verification, and validation in monitoring Salmonella contamination to secure food and food supply chain safety. The technology of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), a gene editing tool acting as precise molecular scissors to cut a target sequence, has incorporated with AbP to detect Salmonella for human health and food safety protection in a CRISPR-Cas12a and biosensing-based, rapid, sensitive, PCR-free fashion. Currently, single-stranded DNA (ssDNA) cleavage and relevant reduced polymer growth have demonstrated the feasibility of the novel CRISPR integrated AbP method.https://digital.library.ncat.edu/gradresearchsymposium25/1188/thumbnail.jp
Analyzing Driver Distraction During Animal Crossings on Rural Roads: Insights from Eye-Tracking and Vehicle in-the-Loop Simulation
No full textRural roads continue to be among the riskiest and least understood driving conditions. Using a vehicle-in-the-loop driving simulator and Tobii Glasses 3 eye-tracking technology, this study examined driving performance and behavior on rural roads in three different visibility scenarios: high (during the day), medium and low(during the night). A comparison of driver attention and decision-making in manual and Advanced Driver Assistance System (ADAS) modes was made possible by the inclusion of actual risks in the simulations, such as roadside distractions and animal crossings considering both animal walking and running cross the road. Important findings showed that drivers’ ability to notice hazards was seriously lacking in areas including failing to keep safe distances and ignoring animal crossings. The unpredictable and low visibility of rural areas, where cognitive load and attention demands were higher, made it difficult for ADAS systems to prevent crashes, even though they were successful in controlled circumstances. These findings highlight the necessity of more sophisticated sensors, cameras, and radars to improve ADAS functionality in rural areas. This work offers important insights into how visual attention, cognitive functions, and driving performance interact. These insights can be used to develop adaptive ADAS systems, improve road safety, and solve the particular difficulties associated with driving in rural areashttps://digital.library.ncat.edu/gradresearchsymposium25/1120/thumbnail.jp
New Farmers of America Association
No full textNFA members visit Oxford Orphanage during special State-wide Fund-Raising Project. Left to right: T. A. Hamme, Superintendent of the Orphanage; S. B. Simmons, Superintendent for Negro Schools; J. T. Locke, Teacher of Agriculture; NFA member, student; and State Supervisor of Vocational Agriculture in North Carolina, Roy H. Thomas. There are food products, a car, and two young men in the background.https://digital.library.ncat.edu/photos/1885/thumbnail.jp
New Farmers of America Association
No full textGroup of men surrounding a container, with a man stirring the materials.https://digital.library.ncat.edu/photos/1918/thumbnail.jp
New Farmers of America Association
No full textA. McMaster\u27s meat hogs.https://digital.library.ncat.edu/photos/1932/thumbnail.jp
New Farmers of America Association
No full textPeople gathered in front of wooden building.https://digital.library.ncat.edu/photos/1955/thumbnail.jp
New Farmers of America Association
No full textYoung boy standing in cornfield. Labeled \u27Chiner Dee.\u27https://digital.library.ncat.edu/photos/1961/thumbnail.jp
New Farmers of America Association
No full textYoung boys raking grass in front of buildings.https://digital.library.ncat.edu/photos/1963/thumbnail.jp
New Farmers of America Association
No full textYoung farmer Edward Whitaker in a field with a cow.https://digital.library.ncat.edu/photos/1987/thumbnail.jp
New Farmers of America Association
No full textSeven men standing on steps in front of a building. S.B. Simmons, standing back row on the right.https://digital.library.ncat.edu/photos/1988/thumbnail.jp