North Carolina Agricultural and Technical State University
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New Farmers of America Association
No full textClass of boys in pruning.https://digital.library.ncat.edu/photos/1956/thumbnail.jp
New Farmers of America Association
No full textPosters of livestock on a wall beneath dried plants.https://digital.library.ncat.edu/photos/1958/thumbnail.jp
New Farmers of America Association
No full textMan standing in a field of crops.https://digital.library.ncat.edu/photos/1978/thumbnail.jp
New Farmers of America Association
No full textA man, woman, and child standing in front of a house.https://digital.library.ncat.edu/photos/1981/thumbnail.jp
New Farmers of America Association
No full textNFA emblem and piano behind a young man standing next to a chair.https://digital.library.ncat.edu/photos/1993/thumbnail.jp
New Farmers of America Association
No full textTwo women standing in front of cars.https://digital.library.ncat.edu/photos/1998/thumbnail.jp
New Farmers of America Association
No full textMacon County Rural School Garden Exhibit. Section of Rural School Exhibit at Tuskegee Institute. Rosenwald School Houses sign in background.https://digital.library.ncat.edu/photos/2015/thumbnail.jp
New Farmers of America Association
No full textFour young men gathered around an older man.https://digital.library.ncat.edu/photos/2017/thumbnail.jp
Differential Expression of MMTV Gene Sequences in Tumors of European and African Ancestry
No full textThe Triple Negative Breast Cancer (TNBC) phenotype constitutes a group of the most heterogeneous tumors. These tumors are clinically aggressive with poor survival rates, lack targeted therapy, tend to be resistant to chemotherapy, and have a high risk of relapse. They account for 15-25% of the breast cancer cases in tumors of African ancestry, resulting in a 40% higher mortality rate. Mouse Mammary Tumor Virus (MMTV) is a retrovirus known to induce mammary tumors in mice by integrating its genetic material into the host genome. In some studies, MMTV-like sequences have been detected in human breast cancer tissues, including TNBC. The differential expression of MMTV genes across diverse ethnic groups could provide insight into the pathogenesis and health disparities associated with TNBC. Utilizing the Galaxy platform for genomic analysis, we could visualize and quantify gene expression in the two groups. We found the differential expression of MMTV (gag, env and sag) genes in TNBC of EA and AA. Expression of the MMTV genes was observed in hotspots. The main gag gene hotspot was predominant in 80% of tumors of African ancestry, as compared to 60% of tumors of European ancestry. The primary env gene was predominant in 60% of the tumors of African ancestry, as compared to 20% of the tumors of European. The primary sag gene hotspot was predominant in 60% of African ancestry tumors, as compared to 20% of European ancestry tumors. It is possible that differentially expressed MMTV genes play a role in pathogenesis and health disparities associated with TNBC.https://digital.library.ncat.edu/honorscollegesymposium25/1032/thumbnail.jp
Development of CRISPR-Integrated Amplification-by-Polymerization for Detection of Salmonella
No full textSalmonella is listed by the Centers for Disease Control and Prevention (CDC) as one of the most common foodborne pathogens and it is a bacterium to trigger symptomatic infections with experiencing diarrhea, fever, and stomach cramps. Genomic DNA from Salmonella as a target analyte can be used for assay development, verification, and validation in monitoring Salmonella contamination to secure food and food supply chain safety. The technology of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a gene editing tool acting as precise molecular scissors to cut a target sequence. He et al. developed an “Amplification-by-Polymerization (AbP)” in biosensing technique based on effective mass growth upon biomolecular recognition using reversible-deactivation radical polymerization reactions and successfully demonstrated direct visualization of DNA, protein, and other biomarkers via AbP processes. This interdisciplinary collaborative research is aimed at developing a novel CRISPR-integrated Amplification-by-Polymerization method to detect Salmonella for human health and food safety protection in a CRISPR-Cas12a and biosensing-based, rapid, sensitive, label-free, PCR-free, and detector-free fashion.https://digital.library.ncat.edu/honorscollegesymposium25/1017/thumbnail.jp