Potravinarstvo Slovak Journal of Food Sciences
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Effect of aero-/anaerobiosis on decarboxylase activity of selected lactic acid bacteria
Full text linkBiogenic amines are undesirable compounds produced in foods mainly through bacterial decarboxylase activity. The aim of this study was to investigate some environmental conditions (particularly aero/anaerobiosis, sodium chloride concentration (0–2% w/w), and amount of lactose (0–1% w/w)) on the activity of tyrosine decarboxylase enzymes of selected six technological important Lactococcus lactis strains. The levels of parameters tested were chosen according to real situation in fermented dairy products technology (especially cheese-making). Tyramine was determined by the ion-exchange chromatography with post-column ninhydrine derivatization and spectrophotometric detection. Tyrosine decarboxylation occurred during the active growth phase. Under the model conditions used, oxygen availability had influence on tyramine production, anaerobiosis seemed to favour the enzyme activity because all L. lactis strains produced higher tyramine amount
Migration of phthalates from plastic tank to vegetable oil as a part of feeding mixtures used for chicken broilers fattening
Full text linkThe concentrations of phthalic acid esters (PAEs) as di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) were measured in samples of rapeseed oil, which was used as a feed. First samples were collected during the production process and second after the storage in plastic tank (21 days). The results of measurements are that there is 2.93-10.10 mg PAEs.kg-1 in the oil before storage and 22.73-61.55 mg PAEs.kg-1 after storage.
For the monitoring of distribution and accumulation of PAEs in animal tissues and organs (muscles, adipose tissue, skin and liver) broiler chicks ROSS 308 were used. The chicks were divided into 4 groups (50 chicks each). All the chicks were fed by commercial diets (complete feed, KKS) for broiler chicks (starter – BR1; grower – BR2 and finisher – BR3). The experimental diets were supplemented with vegetable oil (RO) with low (group N) or high (group V) phthalate content, or animal fat with high phthalate content (group Z). Neither the control diets (K) nor the grower (BR1) diets contained vegetable oil or animal fat. DBP and DEHP were found in all tissues of all chicks. The highest concentration of DBP of 1.28 1.00 mg.kg-1 of fresh sample (an average value from 8 chicks) was determined in the adipose tissue of V chicks. The highest concentration of DEHP of 3.27 2.87 mg.kg-1 of fresh sample (average of 8 chicks) was also determined in the V group
Quality of broiler´s production on the farm in the application of welfare
Full text linkTwo experiments were carried out in practical conditions on the farm for fattening of chickens. In the hall with deep litter, which were carried out experiments, was installations with breeding technology of Big Dutchman with automatic feeding, watering, automatically set to light and temperature regimes. During both experiments were observed recommended microclimate conditions and the length of light and ventilation in the hall. The concentration of broilers per one square meter was 27.22, respectively. 29.34 kg. Their vitality was 94.2 and 95.6%. The average body weight of chickens at the end of the experiment (day 42) were 1986.65 and 2074.29 g, with a statistically proved differences between experiments (P < 0.01). The average carcass weight of broilers in the live weight 1800.0 g were 1384.67 and 1407.0 g, while the difference was not statistically proved (P > 0.05)
Proteins of potatoes in relation to the content of cadmium in their tubers
Full text linkIn the work the influence of cadmium in soil on the range of cumulating in tubers of potatoes and in proteins of potatoes grown under model conditions of pot trial experiment and under the real conditions of locality Imeľ in Danube Lowland was surveyed. Under conditions of pot trial the increased contents in tubers positively correlated with contents of cadmium applied into soil; the highest content of Cd was assessed in variety Junior (from 0.211 mg.kg-1 FM in 1st variant to 0.715 mg.kg-1 FM in 4th variant). The influence of increased content of Cd was manifested statistically significant in the content of proteins also in the content of Cd in protein fractions (1st var. 0.026 (Asterix) – 0.045 (Agria) mg.kg-1 FM; 2nd var. 0.047 (Livera) – 0.085 (Asterix) mg.kg-1 FM; 3rd var. 0.06 (Livera) – 0.117 (Junior) mg.kg-1 FM; 4th var. 0.068 (Livera) – 0.142 (Asterix) mg.kg-1 FM). Contents of Cd in potatoes from locality Imeľ did not exceed the value 0.1 mg.kg-1 FM defined in PK SR. The average contents of proteins were in range from 1.19 % (Victoria) to 1.489 % (Adora), the average content of Cd cumulated in proteins was the highest in variety Vivaldi (1.317 μg.kg-1 FM). Positive correlation was confirmed between the content of Cd in potato tubers and in proteins only in Livera variety