Scientific Journals of INIA (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
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Comparison of insecticidal efficacy of four natural substances against granary weevil (Sitophilus granarius [L.]) adults: does the combined use of the substances improve their efficacy?
Laboratory tests were carried out to evaluate the insecticidal efficacy of different natural inert dusts (diatomaceous earth, wood ash, quartz sand) and the leaf powder of Azadirachta indica A. Juss. against granary weevil (Sitophilus granarius [L.]) adults. The efficacy of the substances was tested individually and in combination with each other. The substances were applied at different concentrations, and bioassays were carried out at four different temperatures (20, 25, 30 and 35°C) and two different relative humidity (RH) levels (55% and 75%). The adult mortality was recorded after the 7th, 14th and 21st days of exposure. The progeny production of individuals exposed to different combinations was also assessed. Wood ash proved to be the most efficient inert dust in our research. We detected 100% mortality in the treatment exposed to a higher concentration (5 w%) of wood ash at 35°C and 55% RH after 7 days of exposure. A lower RH level had also a negative impact on the progeny production. We can conclude that wood ash can be efficient in controlling granary weevil adults as a single substance or in combination with other substances. Further surveys should focus on the impact of the wood ash dose rates. Due to the high percentage of area covered with forest in some European countries, the main ingredient is present locally, but additional surveys are needed to help improve the practical use of wood ash
Short communication: Morphological description and molecular detection of Pestalotiopsis sp. on hazelnut in Serbia
In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia. Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch’s postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-α, and TUB genomic regions. Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut
Damages to soil and tree species by cable-skidding in Caspian forests of Iran
Aim of study: The main aims of this study were to determine of damage level to residual stand and soil disturbance from mechanized selection logging. Area of study: Mixed beech stands in Caspian forests, northern Iran.Material and methods: Point-transect and systematic plot sampling were used for assessing damages to soil and trees, respectively.Main results: 89% of forest soil area was undisturbed or shallow disturbed, and 5.2% was deep disturbed. Soil bulk density of top 10 cm in the winching corridors, ruts and skid trails were increased 10.7%, 20.6% and 32.1% respectively than controlled area. Frequency of damages to regeneration and trees were 12% and 11.2%. The frequency of damages to regeneration was increased with increasing of their heights, but frequency of damages to trees was decreased with increasing of their diameter. The most type of damages was bole wounds in sizes of 100 to 200 cm2 within 1 m from the ground level, and deep wounds. The frequency of damages was different in tree species (p = 0.001). The mean size of bole wounds was 174 cm2, and the mean height of bole wounds was 70 cm from ground level. The intensity of wounds on trees bole were decreased with increasing of their heights from ground level (p = 0.02), while their sizes were increased (p = 0.001).Research highlights: Winching of logs was the main cause of damages to soil and residual stand. The detailed planning strategy will reduce damage to level which is acceptable and predictable
Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity
Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks).Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces).Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution) and lumen fraction (F, the total sapwood area available for water transport) were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate.Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species
Asset-building payments for ecosystem services: assessing landowner perceptions of reforestation incentives in Lebanon
Aim of study: Incentivising landowners to supply ecosystem services remains challenging, especially when this requires long-term investments such as reforestation. We investigated how landowners perceive, and would respond to, distinct types of incentives for planting diverse native trees on private lands in Lebanon. Our aim was to understand landowners’ attitudes towards hypothetical Payments for Ecosystem Services (PES) contracts options; their likely participation; and the potential additionality they would provide. Area of study: Highland villages situated within eight of Lebanon’s 20 Important Plant Areas Materials and methods: Mixed-methods surveys were conducted with 34 landowners to determine past, present and future land-use strategies. Study participants were presented with three differently structured reforestation contract options (or schemes). The three schemes (results-based loan, action-based grant, and results-based payments) differed in their expected risks and benefits to landowners. Qualitative debriefing questions followed each of the schemes presented. Main results: Although the results-based loan did deter uptake relative to the lower risk action-based grant, results-based payments did not significantly increase uptake or planting area, suggesting asymmetric attitudes to risk. Qualitative probing revealed economic, social (e.g. trust) and institutional factors (e.g. legal implications of planting forest trees on private land) that limited willingness to participate in the results-based contract option. Research highlights: This study demonstrates the importance of combining qualitative and quantitative methods to better understand landowner perceptions of incentives and risks, particularly in challenging socio-political contexts
Decline in holm oak coppices (Quercus ilex L. subsp. ballota (Desf.) Samp.): biometric and physiological interpretations
Aim of the study: To analyse the decline in aged holm oak coppice forests as regards above-ground and below-ground fractions and physiological features.Area of study: Centre of the Iberian Peninsula (Guadalajara province).Material and methods: 26 pairs of holm oak stools with different vigour but with similar site and structural characteristics within each pair were selected. Morphological (basal area, number of stools, maximum height) and physiological traits (leaf water potential, stomatal conductance) of the standing stools were assessed. Their aerial and underground parts were extracted and different size fractions of both their above and below-ground biomass were quantified. Linear mixed models were built to test the effect of ’Stool vigour’ on the mean behaviour of the measured variables. Additionally, for the aerial part, linear regressions between the weights of the different size fractions and the basal area at breast height were performed using ‘Stool vigour’ as a fixed factor.Main results: For the same site, root depth, and number and diameter of shoots than good vigour stools, poor vigour stools displayed: lower predawn water potential, greater leaf mass per unit of area; lower total leaf area; lower above-ground biomass (in total as well as per fractions); lower fine roots biomass; lower proportion of leaf biomass and a greater proportion of biomass of both all roots and those with diameter 2-7 cm.Research highlights: The above-ground physiological and morphological characteristics of declined stools are interpreted as poorer adaptation to site conditions. Root system architecture was found to be relevant to explain this behaviour
Natural regeneration in Iberian pines: A review of dynamic processes and proposals for management
Aim of study: Designing adequate silvicultural systems for natural regeneration of a forest species requires sound knowledge of the underlying ecological subprocesses: flowering and fruiting, seed dispersal and predation, seed germination, seedling emergence and seedling survival. The main objective of the present work is to carry out a review on the current knowledge about the different subprocesses governing the regeneration process for the main Iberian Pinus species, in order to propose scientifically based management schedules.Area of study: The review focuses on the five main native Pinus species within their most representative areas in the Iberian Peninsula: Pinus nigra in Cuenca mountains, Pinus sylvestris in Sierra de Guadarrama, Pinus pinaster and Pinus pinea in the Northern Plateau and Pinus halepensis in CataloniaMaterial and methods: Firstly, currently available information on spatiotemporal dynamics and influential factors is introduced for each subprocess and species. Secondly, current regeneration strategies are characterized and the main bottlenecks are identified. Finally, alternative silvicultural practices proposed on the light of the previous information are presented.Main results: Different climate-mediated bottlenecks have been identified to limit natural regeneration of the Iberian pine species, with seed predation and initial seedling survival among the most influential. New approaches focusing on more gradual regeneration fellings, extended rotation periods, prevent big gaps and program fellings on mast years are presented.Research highlights: Natural regeneration of the studied species exhibit an intermittent temporal pattern, which should be aggravated under drier scenarios. More flexible management schedules should fulfil these limitations.