Scientific Journals of INIA (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
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Short communication: Broadening the diagnosis panel of reproductive pathogens associated with abortion in ruminants
Get PDFDiagnosis of abortion in cattle, sheep and goat have been mainly focused on abortive pathogens with a recognized impact in outbreaks, but the aetiologic diagnosis rates have been historically low worldwide. Thus, we analysed the presence of pathogens in abortion outbreaks, focusing on the less-common pathogens in cattle farms with control programmes for reproductive pathogens, and in ovine and caprine farms. Thirty-one cases from Galician farms submitted to our laboratory during 2013-2015 were analysed (16 bovine, 7 ovine and 8 caprine farms) by polymerase chain reaction and culture from foetal tissues (n = 52 foetuses). Diagnosis was reached in 21/31 farms: 9/16 in bovine, 6/7 in ovine and 6/8 in caprine. Campylobacter spp. were found in all three species (3/9 diagnosed cases in bovine, 2/6 in ovine and 4/6 in caprine). Furthermore, Ureaplasma diversum was detected in cattle (4/9 of diagnosed cases), Bovine Viral Diarrhoea Virus – 2 was detected in sheep (2/6) and Neospora caninum in goats (1/6). Our results prove the occurrence of abortion in response to pathogens that are traditionally considered less relevant and rarely included in the diagnosis of ruminant abortion. Therefore, differential diagnosis of abortion should consider these pathogens (at least when other causes have been ruled out), to effectively control abortion in farms
Growth and nutrients content of Atriplex canescens across a soil electric conductivity gradient
Get PDFAtriplex canescens canopy cover, biomass production, and nutrient content were evaluated after four years of livestock grazing exclusion and mechanical shrub removal, except A. canescensin an arid rangeland with a slightly saline soil and with a long history of heavy livestock grazing in the southern Chihuahuan Desert, Mexico (24° N). Twenty 3 × 3 m paddocks were established in terrains with three levels of electric conductivity (EC): <1.4 dS/m (n=7), 1.4–1.6 dS/m (n=7) and >1.6 dS/m. Atriplex canescens canopy cover was higher (49.5%; p<0.01) on paddocks with soil EC >1.6 dS/m than paddocks with soil EC <1.4 and 1.4–1.6 dS/m (32.1 and 22.9%, respectively). Above-ground biomass did not differ between paddocks with soils with EC of <1.4 and 1.4–1.6 dS/m (1309 ± 535 and 1372 ± 180 kg DM/ha), but biomass increased 2.7 times (p<0.01) when soil EC was greater than 1.6 dS/m. The soil EC had no effect on the levels of crude protein (range 13.6 to 14.3%), neutral detergent fiber (range 56.5 to 57.7%) and ash (range 14.5 to 16.4%). In vitro dry matter digestibility (IVDMD) of the foliage of A. canescens was not affected by of soil EC level (range 60.4 to 62.2%). It was concluded that in an arid rangeland with slight saline soil, the increase in salinity favors canopy cover and biomass production of A. canescens without altering nutrient content and IVDMD of this fodder shrub
Genetic variability assessment in ‘Muscat’ grapevines including ‘Muscat of Alexandria’ clones from selection programs
Get PDFGenetic variability is needed to face environmental changes and pathogen constrains. In addition, the search for intravarietal variability contributes to the avoidance of genetic erosion, preserving clones that are adapted to particular conditions. Variability is also important to diversify grapevine-derived products. In this work, we have analyzed the genetic variability of ‘Muscat germplasm’ including samples from neglected vineyards from Alicante and Valencia provinces, accessions of the germplasm collections of ‘Colección de Vides de El Encín’ (Alcalá de Henares, Madrid) and ‘La Casa de las Vides’ (Agullent, Valencia), accessions supplied by nurseries of Valencia province, and ‘Muscat of Alexandria’ clones selected using differential ampelographic characteristics in selection programs (La Marina, Alicante). Fifteen microsatellites (SSRs) were used to study intervarietal variability. The SSR fingerprinting allowed the identification of some accessions, variants, and synonymies. Amplified Fragment Length Polymorphisms (AFLPs) markers and Microsatellite-AFLPs were used to determine the variability attended in ‘Muscat of Alexandria’ accessions. A CAPs (Cleaved Amplified Polymorphic Sequences) marker, recently developed for the discrimination of ‘Muscat’ flavor genotypes using the SNP1822 G>T, was assessed and showed that all the analyzed accessions were ‘Muscat’ flavored. The variation found among the analyzed germplasm is very interesting because variants within ‘Muscat of Alexandria’, ‘Muscat Italia’, and ‘Muscat d’Istambul’ have been identified. In addition, intravarietal genetic variation was found among the analyzed accessions in ‘Muscat of Alexandria’ from selection programs
Performance, carcass characteristics, economic margin and meat quality in young Tudanca bulls fed on two levels of grass silage and concentrate
Get PDFThis study investigates the effect on performance, carcass and meat characteristics of increasing the forage level in the diet of fattening Tudanca young bulls using silage as the forage source as compared with a conventional ad libitum straw plus concentrate diet. Twenty two Tudanca young bulls were assigned to three different finishing diets: ad libitum grass silage plus ad libitum concentrate (GS-AC), ad libitum grass silage plus concentrate limited to a half of the intake of the ad libitum group (GS-LC), ad libitum barley straw plus ad libitum concentrate (Str-AC) and then slaughtered at around 11 months of age. GS-LC diet resulted in relation to GS-AC and Str-AC diets in lower (p≤0.05) average daily weight gain (750 vs 1,059 and 991 g/animal/day, respectively), lower (p≤0.05) carcass weight (133 vs 159 and 152 kg, respectively) and carcasses with slightly lower conformation scores. Although GS-LC diet allowed for a lower dependence on concentrate (372 vs 657 and 729 kg/animal, respectively), economic margin was similar for the two GS groups (-63.1 and -64.1 vs -91.8 €/head). The polyunsaturated/saturated fatty acid ratio was the lowest (p≤0.05) in GS-AC meat (the group showing the highest IMF levels) and the ratio n-6/n-3 was the highest (p≤0.05) in Str-AC meat. GS-LC meat showed higher collagen content and Str-AC meat presented higher cohesiveness, springiness and chewiness values. Results suggested that the substitution of straw by grass silage and the restriction of the concentrate level could be recommended for finishing young Tudanca bulls in indoors systems
Risk model of tree stand damage by winds and its evaluation based on damage caused by cyclone “Xaver”
Get PDFAim of study: To develop and evaluate the forest’s wind-risk model, dedicated for stand damage level.Area of study: Model was tested in the northeastern Poland.Material and methods: A risk model referring to the damage of forest stands by wind specifies, for every stand in a selected forest district, the risk factor within the range of 0 to 3. The higher value of the factor, the greater risk of damage, if wind occurs. The model was based on 11 features: average size of a tree stand, mean diameter breast high in the specified features’ ratio, species composition, degree of stand density, age of stand, forest site type, amount of damage caused by wind in the last 10-year period, location of forest district in the region of Poland, and three features for mountains: stand’s altitude above sea level, direction, and slope. The model used information from the State Forests’ Information System database (SILP), and since it was developed in JAVA computer language, the processing of data for one forest district lasted less than one minute.Main results: The model can create a forest district digital map, in which stands characterized by specific risk values are presented with high prediction accuracy.Research highlights: The risk model of tree stand damage by winds uses data provided by the SILP and what was proven in below study, can be used as an effective tool in a forestry practice
Influence of timber harvesting costs on the layout of cuttings and economic return in forest planning based on dynamic treatment units
Get PDFAim of study: To analyze the influence of harvesting costs on the distribution and type of cuttings when forest management planning is based on the dynamic treatment units (DTUs) approach.Area of study: A Mediterranean pine forest in Central Spain.Materials and methods: Airborne laser scanning data were used in area-based approach to predict stand attributes and delineate segments that were used as calculation units. Predicted stand attributes and existing models for diameter distribution and individual-tree growth were used to simulate alternative management schedules for each segment for a 60-year planning horizon divided into three 20-year periods. Three alternative forest planning problems were formulated. They aimed to maximize or minimize net income, or maximize timber production with a constant flow of harvested timber. Spatial goals were used in all cases to enhance the clustering of treatments.Main results: Maxizing timber production without considering harvesting costs can be costly, even close to the plan that minimized net incomes. Maximizing net incomes led to frequent use of final felling instead of thinnings, placing cuttings near forest roads and creating more compact DTUs than obtained in the plan that maximized timber production.Research highlights: Compared to previous studies on DTUs, this study integrated felling and forwarding costs, which depended on distance to road and stand attributes, in the process of creating DTUs by means of spatial optimization
Resource communication: ApkFor©, an Android Open-Source Project for research and technology transfer in forest management
Get PDFAim of the study: To introduce and describe ApkFor©, an Android Open-Source Project to generate basic mobile applications to transfer forest growth and yield models for even-aged stands.Material and methods: ApkFor© was developed in Android Studio using Java and XML languages integrating transition functions for dominant height and basal area, equations of tree and stand volume and structural models. The project was applied and validated for Pinus pinaster Ait. stands in Northeastern Portugal.Main results: ApkFor© is an Open-Source project freely available from the Source Force repository: https://sourceforge.net/projects/apkfor/, licensed under the GNU General Public License version 3.0 (GPLv3). Research highlights: This project has been designed and created to provide the code and promote its re-use and modification to develop simple growth and yield mobile applications in Android, and with it to transfer research results of forest modelling to forest managers. Moreover, an example of application of the compiled code is provided using the models of Pinus pinaster Ait. previously validated for the Northeastern Region of Portugal