Scientific Journals of INIA (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
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A quantitative multivariate methodology for unsupervised class identification in pistachio (Pistacia vera L.) plant leaves size
Full text linkAim of study: Genetic diversity of pistachio, can be evaluated by using different descriptors, as adopted in international certification systems. Mainly the descriptors are morphological traits as leaf, which represents an important organ for its sensibility to growth conditions during the expansion phase. This study adopted a rapid and quantitative non-hierarchic clustering classification (k-means), to extract size classes basing on the contemporary combination of different morphological traits (i.e., leaf stalk length, terminal leaf length, terminal leaf width and terminal leaf ratio) of a varietal collection composed by 21 pistachio cultivars.Area of study: Worldwide.Material and methods: The unsupervised non-hierarchic clustering technique was adopted to the entire samples of pistachio leaves from k=2 to k=15 for both four morphological variables (i.e., leaf stalk length, terminal leaf length, terminal leaf width and terminal leaf ratio) and three morphological variables (i.e., terminal leaf length, terminal leaf width and terminal leaf ratio).Main results: A classification model only on the three morphological variables (for results of statistical analysis in which the groups resulted to be more separated and different for all the variables), with k= 5 (five groups), was constructed using a non-linear artificial neural network approach. The percentages of bad prediction in both training and testing resulted equal to 0%. The “terminal leaf length” returned the higher impact (44.89%).Research highlights: The contemporary combination of different morphological leaf traits, allowed to create an automatic classification of size classes of great importance for cultivar identification and comparison
Economic valuation of rangelands in the north of Mexico: A study for its conservation
Full text linkAim of study: To estimate grazing fee and the economic value of the communal rangelands of two groups of ranchers.Area of study: Ejido Tanque de Arenas (ETA) and ejido Tanque de Dolores (ETD) in Catorce, San Luis Potosí, Mexico.Material and methods: The contingent valuation method with referendum format was used to propose a grazing fee that would allow conservation and continuity in the use of the common resource. The field information was collected by interviews and was processed in the PASW Statistics 22 software NLOGIT Version 5.0.Main results: Perception of rangeland deterioration, age, size of the herd, and level of income are characteristics of ranchers that influence the willingness to pay. From the coefficient of rangeland of the region (24.2 ha/AU) a monthly grazing fee of USD 7.18 per animal was calculated.Research highlights: Estimation and payment of grazing fees are an alternative to avoid the deterioration of communal rangelands, without incurring land parcelization
Phenolic profile of grapevine cv. Tempranillo skins is affected by timing and severity of early defoliation
Full text linkAim of study: To investigate the effects of three early leaf removal treatments on the phenolic compounds of cv. ‘Tempranillo’ (Vitis vinifera L.) grape skins.Area of study: The experiment was conducted in a vineyard located in Requena, Valencia (South-eastern Spain) over two consecutive seasons.Materials and methods: Four treatments were investigated over two seasons in drip-irrigated vines: Control (C), non-defoliated and three defoliation treatment, applied at different phenological stages and intensities where all leaves from the first 6 nodes were eliminated just before flowering (ED) and at fruitset (LD). The fourth defoliation treatment was performed at the same time of ED but only the leaves facing east of the eight first nodes were removed (EED). At harvest, thirty-eight phenolic compounds were quantified by HPLC in the grape skins, including anthocyanins, flavanols, flavonols, hydroxycinnamic acids and their tartaric derivatives.Main results: A general increase of the skin phenolic compounds concentration was found in response to the defoliation treatments. The largest and more significant effects were observed for LD in 2009 with relative increases with respect to the un-defoliated vines of 14.8, 86.0, 119.0, and 75.9% for anthocyanins, flavanols, flavonols and hydroxycinnamates, respectively. On the other hand, EED did not clearly modify any polyphenolic compound. In addition, the response of phenolic families analyzed to defoliation treatments was different. Malvidine derivatives were not altered by any of the treatments, while the contents of quercetin and kaempferol derivatives and ferulic and coumaric acids, increased in both years when LD was applied.Research highlights: The defoliation effects on specific phenolic substances were dependent on timing, severity, and the season. Skin phenolic compounds increase in response to defoliation treatments and flavonols and hydroxycinnamates were the most affected families
The impact of alternative feeding strategies on total factor productivity growth of pig farming: Empirical evidence from EU countries
Full text linkAim of study: To investigate the impact of adopting new feeding precision technology on pig production.Area of study: Four EU countries (Germany, France, Poland and Spain) during the period 2010–2015.Material and methods: The Färe-Primont index was used to estimate total factor productivity change and its components, technological change and efficiency change.Main results: German, French and Spanish farms experienced total factor productivity (TFP) progress, while Polish farms did not for both feeding strategies. Our empirical findings suggest a high impact on the productivity of ad libitum feeding technique compared to the restricted one for all countries.Research highlights: Precision feeding strategies provide another avenue to more sustainable livestock production and further evidence that implementing individual ad libitum feeding systems for pigs could enhance farm’s productivity
New validated Eucalyptus SSR markers located in candidate genes involved in growth and plant development
Full text linkAim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species.Area of study: Eucalyptus species from different Australian origins planted in Argentina.Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii.Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest.Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.Keywords: CG-SSR; cross-transferability; EST; eucalypts; microsatellite
Evaluation of physical and chemical soil properties under different management types in the south-western Colombian Andes
Full text linkAim of study: Evaluating the variability of physical and chemical soil properties under different soil uses in an experimental farmland of the southwestern Colombian Andes.Area of study: This research was conducted at the Botana Experimental Farm in the mountainous area of Nariño, at the south-western Colombia.Material and methods: nine soil variables were measured under six soil uses which included traditional agriculture, agroforestry systems, and a 67-year-old secondary forest that was used as a reference for comparisons with other soil uses. Data was analyzed through Principal Component Analysis and Duncan’s tests.Main results: organic carbon, cation exchange capacity, clay contents and base saturation were the variables with higher variability among soil uses. The secondary forest and an agroforestry system with alley-cropped wax laurel showed the best soil conditions, whilst pastures and monoculture potato crop plots showed the least desirable conditions for all variables.Research highlights: We found that soils under alley-crop with wax laurel presented the characteristics most similar to the secondary forest. Conversely, soils under alley-crop with alder resembled the soils under intensive management (pasture and potato monocrop); which is related to the inadequate management of this agroforestry systems, provoking that the woody component does not accomplish its goal when implemented.Keywords: soil; agroforestry systems; Andes; forest.Abbreviations used: ACAL, Alley cropping of Alder Trees, ACWL; Alley cropping of Wax Laurel; AFS, agroforestry systems; AWC, available water capacity; BD, Bulk Density; BS, Base saturation; CEC, Cation Exchange Capacity; CLA, Clay; CI, Confidence Intervals, INF, Infiltration; ANOVA, Analysis of Variance; OC, Soil Organic Carbon; PAST, Pasture; PCA, Principal Component Analysis; PC, Principal Component; POCR, Potato Crop; POR, Soil Porosity; SCAL, Scattered Alder Trees; SEFO, Secondary Forest; SU, soil uses; AU, Animal unit
Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes
Full text linkAim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates.Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia).Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world.Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RT-PCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain.Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia
Oral and contact toxicity of the extract obtained with hexane from Achyrocline satureioides on larvae and adult honey bees
Full text linkAim of study: To evaluate the antimicrobial activity of the hexanic extract (HE) of Achyrocline satureioides on Paenibacillus larvae - a gram-positive spore-forming bacillus that affects the larval stage and causes American Foulbrood (AFB) - and its oral and contact toxicity on larvae and adult honey bees.Area of study: A. satureioides plants were collected in Santa Monica (32° 05’ 29” S, 64° 36’ 54” W, Córdoba. Argentina). The larvae and adults of Apis mellifera were obtained from the experimental apiary of the University of Córdoba, Spain.Material and methods: P. larvae 9 was previously isolated and identified in the Laboratory of General Microbiology (Dept. of Microbiology, National University of Río Cuarto, Argentina). The HE was obtained by liquid-liquid extraction. The minimum inhibitory concentration (MIC) of HE was determined by a microdilution method. This concentration and 2 ½ MIC were used for in vitro toxicity tests. Oral toxicity was tested on larvae, feeding them with both concentrations of the HE, while on adult bees the HE was spread to determine contact toxicity.Main results: The HE showed antimicrobial activity, the MIC obtained was 0.4 μg/mL. The HE presented very low toxicity at the MIC and 2 ½ MIC, with survival percentages to be around 95% for adult bees and larvae.Research highlights: The results show that this extract could be used for the development of an alternative product for a safe and effective treatment of AFB
Electrocution and containment methods to reduce the activity of red palm weevil (Rhynchophorus ferrugineus, Ol.)
Full text linkAim of study: To evaluate the susceptibility of the Rhynchophorus ferrugineus to electric discharges, to eventually use this application in synergy with other methods as part of an integrated control strategy.Area of study: Worldwide.Material and methods: Three different electric voltage intensities were applied to R. ferrugineus to investigate the insect’s susceptibility in both adult and larval stages. The transmission and diffusion of electricity within a portion of the palm tree was tested. In addition, the suitability of containment materials for handling and transportation of plant residues was tested.Main results: The results of adult test showed that the total number of eggs laid per mating couple and the number of larvae born were about 1.5 times higher in the control compared to the electrified samples. The duration of the electric discharge on larvae had a small impact on the mortality rate, while the electric voltage showed a strong impact on mortality (87% for 10 V and 99% for 15 V). The presence of a significant electric current was observed in a viable portion of stem (distance from the source of electricity 10 cm) providing a direct current with 250 V tension. Among the materials tested for the transportation of palm residues, the aluminium net sheet showed the best results in the containment of both larval and adult individuals.Research highlights: Electrocution has proven to be a system which can help in the control of red palm weevil reducing the use of synthetic chemicals