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Targeted gene expression study of Salmonella enterica during biofilm formation on rocket leaves
Get PDFIn the present study, the ability of Salmonella Typhimurium to form biofilm community on rocket leaves and rocket extract at 10 C and 20 C was investigated. This goal was achieved with the study of expression of genes associated with biofilm formation and other functional roles. The obtained results showed that Salmonella growth was inhibited when cultured in rocket extract (liquid and solid state) and when grew directly to rocket leaves. The observed inhibition might be attributed to nutrient starvation to the specific growth media because of plant leaves's variability, cell physiology and antimicrobial compounds of rocket. In addition, gene expression study using Real-Time PCR showed that biofilm was formatted on solid media, while the entrance and adhesion of the microorganism within the plant held more strongly through the stomata of the plant leaves. Furthermore, genes associated with managing stress situations were overexpressed at 20 C. From these results, it is indicated that further studies are needed to better determine the survival and/or growth of the pathogen as “real” biofilm cells on plants. In addition, the study on development and gene expression of biofilm cells is necessary in order to eliminate the specific pathogen and reduce the food-borne diseases it causes
Effects of flavonoids dietary supplementation on milk antioxidant capacity in sheep
Get PDFHesperidin and naringin are bioflavonoids known for their antioxidant properties. They are abundant in inexpensive by-products of citrus cultivation such as citrus pulp which often are treated as waste. An experiment was conducted to examine the effects of dietary hesperidin or naringin supplementation on lactating ewes’ milk antioxidant capacity
Ability of Salmonella enterica and Staphylococcus aureus to develop biofilm community on stainless steel and colonize rocket tissue
Get PDFIn the present study, the ability of S. Typhimurium (CDC 6516-60) and S. aureus strain COL (MRSA) to both develop a biofilm community on stainless steel (SS) and colonize rocket tissue was investigated (incubation at 20°C for 144 h). In parallel, the planktonic growth of these pathogens in Brain Heart Infusion (BHI) broth, was followed
Επίδραση των φυσικών αντιοξειδωτικών Εσπεριδίνη και Ναρινγίνη στην έκφραση γονιδίων στο λιπώδη ιστό και το ήπαρ ορνιθίων κρεοπαραγωγής
Get PDFP3-21 Comparative Proteomic Analysis of Salmonella enterica serovar Enteritidis PT4 Planktonic and Sessile Cells on Stainless Steel Surface Provides New Insights in Protein Determinants Involved in the Maintenance of a Biofilm community
Get PDFIn order to better understand the cellular mechanisms sustaining a surface-associated lifestyle of S. Enteritidis in food related environments, the differential protein patterns of this bacterium cultivated as biofilm on SS versus planktonic mode were comparatively studied in the present work
A new late Miocene (Tortonian) flora from Gavdos Island in southernmost Greece evaluated in the context of vegetation and climate in the Eastern Mediterranean
Get PDFA new late Miocene (Tortonian) leaf flora has recently been recovered in the southernmost part of Europe on the island of Gavdos, Greece. So far, three conifers (Tetraclinis salicornioides, Taxodium dubium and Pinus sp.) and 27 fossil species/morphotypes of angiosperms have been recognized. Among them, some represent subtropical, partly evergreen woody elements (e.g. Daphnogene sp., Laurophyllum sp., Myrica lignitum), some others (e.g., ?Sassafras, Fagus gussonii, Ulmus, Acer angustilobum, Populus populina, Ailanthus pythii, Paliurus tiliifolius, several legumes) belong to deciduous shrubs or trees. The number of the determined angiosperms is sufficient for using statistical techniques to estimate palaeoclimate (LMA, CLAMP, CA) and to reconstruct the palaeovegetation (phytosociological approach, IPR-vegetation analysis). The Gavdos flora is based on leaf impressions and allows correlations with other Greek floras of almost the same age, namely from Crete – Vrysses (latest Tortonian to Messinian, ca. 7.5-6.0 Ma), Makrilia (late Tortonian, ca. 8.6-7.7 Ma) and Pitsidia, Messara Basin (early Tortonian, ca. 10.5 Ma), while that from Vegora, Macedonia (Messinian, ca. 7-6 Ma) may document further trends in the late Miocene
Targeted and non-targeted liquid chromatography-mass spectrometric workflows for identification of transformation products of emerging pollutants in the aquatic environment
Get PDFIdentification of transformation products (TPs) of emerging pollutants is challenging, due to the vast number of compounds, mostly unknown, the complexity of the matrices and their often low concentrations, requiring highly selective, highly sensitive techniques.Wecompile background information on biotic and abiotic formation of TPs and analytical developments over the past five years. We present a database of biotic or abiotic TPs compiled fromthose identified in recent years.We discuss mass spectrometric (MS) techniques and workflows for target, suspect and non-target screening of TPs with emphasis on liquid chromatography
coupled to MS (LC-MS). Both low- and high-resolution (HR) mass analyzers have been applied, but HR-MS is the technique of choice, due to its high confirmatory capabilities, derived fromthe high resolving power and the mass accuracy in MS and MS/MS modes, and the sophisticated software developed
Σύστημα Qu.a.sys: Αναλυτική έκθεση περιγραφής του συστήματος και των εργαλείων του
Get PDFΣκοπός του εγγράφου είναι να παρουσιαστεί αναλυτικά η λειτουργικότητα του υπό ανάπτυξη συστήματος και των εργαλείων το
Identification of unknowns in real wastewater through the application of a LC-QTOF-MS based workflow
Get PDFWastewater contains a high number of organic micropollutants and transformation products of environmental concern. Recent approaches, combining methodologies based on target and suspect screening (for suspected substances based on prior information but with no reference standard) are important for the comprehensive characterization of environmental samples. Nevertheless, samples still contain many chromatographic peaks which do not correspond to substances included in target and suspect screening lists. These substances may be potentially relevant (e.g. due to their concentration or potential effects) and thus the identification of selected non-targets is important. However, full identification of unknown compounds is often difficult and there is no guarantee of a successful outcome. The aim of this work is to show some specific examples on the identification of unknown compounds in real wastewater (collected from the WWTP of Athens). Identifications were conducted using a developed integrated workflow based on LC–QToF-MS to detect formerly unknown organic contaminants in wastewater